Analysis of the growth of mutants on different carbon sources

The phenotyping which we have undertaken consists of detecting variations in growth characteristics on semisolid media between different mutants from our collection. This medium is a so-called "minimal" medium which has been supplemented with one or more metabolites which have been identified as intermediates in a given biosynthetic pathway. High throughput phenotyping of the mutant collection on 24 carbon sources is currently in progress.

On the left : Spotting of 1152 A. baylyi mutants per plate.  Each plate of gelose contains a particular source of carbon. On the right : One of the plate of gelose after 24h of growth.

This analysis will allow classification of mutants which present a phenotype which differs from wild type in the same metabolic context (i.e. metabolic pathways). An example concerning genes implicated in the glucose assimilation pathway is illustrated in the figure below.

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