Nanopore cDNA (PCS108_LR)
| Sample |
brain C1 |
brain C2 |
brain C2 |
liver C1 |
| Number of reads |
1,267,830 |
5,834,882 |
3,003,844 |
3,043,572 |
| Cumulative size (Gb) |
1.30 |
7.03 |
3.28 |
3.30 |
| Average Size (bp) |
1,028.94 |
1,204.54 |
1,091.85 |
1,083.85 |
| N50 (bp) |
1,283 |
1,749 |
1,591 |
1,264 |
| Number of reads (>1Kb) |
522,422 |
2,869,633 |
1,339,489 |
1,218,569 |
| Link to ENA |
ERX2695238 |
ERX3387950 |
ERX3387952 |
ERX2695243 |
| BAM file (transcriptome Ensembl 94) |
bam |
bam |
bam |
bam |
| BAM file (genome) |
bam |
bam |
bam |
bam |
| BAM file (Lexogen SIRVs spike-in only) |
bam |
bam |
bam |
ND |
1. Reads were aligned on the Ensembl 94 transcriptome using minimap2 and the following commande line :
minimap2 -t 12 -ax map-ont Mus_musculus.GRCm38.E94.cdna.all.fa Reads.fa
and alignments were filtered using the following python script :
filter_out_reads_with_less_than_80QC.py
2. Alignment were transfered from transcriptome-coords to genome-coords using the following python script :
ali_transcriptome2genome_v3.py