Genoscope is undertaking the sequencing of 200 fosmids from a metagenomic library created from total DNA from a bacterial fraction associated with healthy human ileal mucosa.
r each fosmid, a plasmid library (3 kb inserts) will be created in pcdna2.1 and 1,500 clones from each library will be sequenced at both ends, for an expected sequencing volume of 600,000 reads.
All the fosmids will be assembled in phase 2, and after analysis, certain interesting fosmids will undergo a finishing stage.