Two strains of Acinetobacter baumannii are being sequenced in Genoscope : a nosocomial strain which is antibiotic multiresistant (VEB) and a community, multisensitive strain (SDF). The genome size for each strain is estimated at 4 Mb. Sequence coverage for each genome is 12X. Three libraries are being sequenced : 30 000 clones with 3 kb inserts in a pNAV vector (high copy number shuttle vector between E. coli and Acinetobacter), 11 500 clones with 10 kb inserts in a pCNS vector (low copy number) and nearly 4000 clones with 20-25 kb inserts in a miniBAC vector (pBBC). A manual finishing stage will be performed at Genoscope.