NaS datasets

Acinetobacter baylyi ADP1 (Sequences from Genoscope)

Reads aligned with reference	Number of reads	Number of reads > 10 Kb	Cumulative size(Mbp)	Average size(bp)	N50 size(bp)	Maximum size(bp)	Number of aligned reads	Mean identity percent	Maximum alignment size	Number of error-free reads
MinION reads aligned using LAST	89 011	14 947	381.4	4 284.5	13 376	137 043	29 954 (33.65%)	70.0883%	84 914	0 (0%)
NaS reads (fast mode) aligned using bwa mem	24 063	8 349	212.7(59X)	8 839.6	12 389	85 162	24 063 (100%)	99.9891%	85 162	22 864 (95.01%)
NaS reads (sensitive mode) aligned using bwa mem	28 492	11 252	271.6(75X)	9530	13 256	91 255	28 492 (100%)	99.9897%	91 255	27 049 (94.93%)

We generated A. baylyi NaS reads using 1D and 2D reads of 6 MinIon runs and 50X of 250bp Illumina reads.

Escherichia coli K-12 substr. MG1655 (Sequences from Loman Lab)

Reads aligned with reference	Number of reads	Number of reads > 10 Kb	Cumulative size(Mbp)	Average size(bp)	N50 size(bp)	Maximum size(bp)	Number of aligned reads	Mean identity percent	Maximum alignment size	Number of error-free reads
MinION reads aligned using LAST	22 270	4 406	133.6	5 999.4	9 356	47 133	22 170 (99.55%)	79.4559%	47 133	0 (0%)
NaS reads (fast mode) aligned using bwa mem	21 818	7 233	172.9(37X)	7 925.5	10 489	48 463	21 818 (100%)	99.9884%	48 463	20 153 (92.36%)
NaS reads (sensitive mode) aligned using bwa mem	22 144	8 146	183.9(40X)	8 307	10 931	48 638	22 144 (100%)	99,9904%	48 638	20 452 (92,35%)

We generated E. coli NaS reads using 2D reads of MinIon runs and 50X of 300bp Illumina reads.

Saccharomyces cerevisae W303 (Sequences from Schatz Lab)

Reads aligned with reference	Number of reads	Number of reads > 10 Kb	Cumulative size(Mbp)	Average size(bp)	N50 size(bp)	Maximum size(bp)	Number of aligned reads	Mean identity percent	Maximum alignment size	Number of error-free reads
MinION reads aligned using LAST	267 768	34 300	1 465.6	5 473.3	7 937	146 992	68 215 (25.47%)	55.4937%	49 077	0 (0%)
NaS reads (fast mode) aligned using bwa mem	71 793	6 141	426.3(34X)	5 938.3	7 085	45 745	71 614 (99.75%)	99.9246%	39 954	56 991 (79.58%)
NaS reads (sensitive mode) aligned using bwa mem	85 432	13 270	578.4(47X)	6 770	8 427	46 159	85 289 (99.83%)	99.9427%	46 159	66 330 (77.77%)

We generated S. cerevisae NaS reads using 1D (only template) and 2D reads of MinIon runs and 50X of 250bp Illumina reads.