Nanopore RNA (RNA001_LR)

Sample brain C1 brain C2 brain C2 liver C1
Number of reads 571,088 364,041 210,654 418,102
Cumulative size (Gb) 0.43 0.38 0.20 0.34
Average Size (bp) 758.05 1,032.10 957.17 823.30
N50 (bp) 1,357 1,492 1,417 1,153
Number of reads (>1Kb) 154,735 141,970 73,232 117,047
Link to ENA ERX2695236 ERX3387949 ERX3387951 ERX2695240
BAM file (transcriptome Ensembl 94) bam bam bam bam
BAM file (genome) bam bam bam bam
BAM file (Lexogen SIRVs spike-in only) bam bam bam ND
1. Reads were aligned on the Ensembl 94 transcriptome using minimap2 and the following commande line :
minimap2 -t 12 -uf -ax map-ont Mus_musculus.GRCm38.E94.cdna.all.fa Reads.fa
and alignments were filtered using the following python script : filter_out_reads_with_less_than_80QC.py
2. Alignment were transfered from transcriptome-coords to genome-coords using the following python script : ali_transcriptome2genome_v3.py