LETTERS
A First-Generation Whole Genome-Radiation Hybrid Map Spanning the Mouse Genome

¹ Genetics Department, University of Cambridge, Downing Site, Cambridge CB2 3EH, UK; ² SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Harlow, Essex CM19 5AW, UK; ³ Millenium Pharmaceuticals, Cambridge, Massachusetts 02139; ⁴ Research Genetics, Inc., Huntsville, Alabama 35801

We have assembled a first-generation anchor map of the mouse genome using a panel of 94 whole-genome-radiation hybrids (WG-RHs) and 271 sequence-tagged sites (STSs). This is the first genome-wide RH anchor map of a model organism. All of the STSs have been previously localized on the genetic map and are located 8.8 Mb apart on average. This mouse WG-RH panel, known as T31, has an average retention frequency of 27.6% and an estimated potential resolution of 145 kb, making it a powerful resource for efficient large-scale expressed sequence tag mapping.

[All of the mapping data for the maps presented here have been deposited at the Research Genetics, Inc., web site and can be freely accessed and downloaded at http://www.resgen.com/.]