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What is SignalP ?

SignalP predicts the presence and location of signal peptide cleavage sites in amino acid sequences from different organisms: Gram-positive prokaryotes, Gram-negative prokaryotes, and eukaryotes. The method incorporates a prediction of cleavage sites and a signal peptide/non-signal peptide prediction based on a combination of several artificial neural networks and hidden Markov models.


Improved prediction of signal peptides: SignalP 3.0. Jannick Dyrløv Bendtsen, Henrik Nielsen, Gunnar von Heijne and Søren Brunak. J. Mol. Biol., 340:783-795, 2004.

How to read SignalP results

  • The first column indicates the type of bacteria (Gram positive or Gram negative).
  • The second column gives the estimated probability (number between 0 and 1) that the sequence contains a signal peptide.
  • The last 2 columns indicate the positions between which the cleavage is supposed to occur.

Tips: A signal peptide has a average size of 30 aa.