BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 496abc7fa76d5f65db9947beca56c571.SwissProt.fasta
3 sequences; 1,154 total letters
Query= Z3175
Length=353
Score E
Sequences producing significant alignments: (Bits) Value
P33015 Thiosulfate transporter TsuA [Escherichia coli (strain K12)] 697 0.0
P31064 Probable transporter YedE [Escherichia coli (strain K12)] 86.3 1e-22
Q06400 Probable transporter YedE [Salmonella typhimurium (strain ... 84.7 3e-22
>P33015 Thiosulfate transporter TsuA [Escherichia coli (strain
K12)]
Length=352
Score = 697 bits (1799), Expect = 0.0
Identities = 345/352 (98%), Positives = 351/352 (99%), Gaps = 0/352 (0%)
Query 1 MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAISVQSVGVFALI 60
MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYI KNNRMFYALLIAISVQSVGVFALI
Sbjct 1 MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIVKNNRMFYALLIAISVQSVGVFALI 60
Query 61 QAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTYMVMS 120
QAGLLTYEAGAFPWLGTVIGGY+FGLGIVLAGGCATGTWYRAGEGLIGSWIALFTYMVMS
Sbjct 61 QAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTYMVMS 120
Query 121 AVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVLLVITLWVVMKELKKPKLKVA 180
AVMRSPHASGLNQTLQHY+TEHNSIAETFNLSVWPLVAVLLVITLWVVMKELKKPKLKVA
Sbjct 121 AVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVLLVITLWVVMKELKKPKLKVA 180
Query 181 TLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGITSPTANILQFL 240
TLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGITSPTANILQFL
Sbjct 181 TLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGITSPTANILQFL 240
Query 241 VAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRAADAQTTVRSGLGGVLMGFGASIAG 300
VAGD+KYINWGVFLVLGIFVG+FIAAKASREFRVRAADAQTT+RSGLGGVLMGFGASIAG
Sbjct 241 VAGDMKYINWGVFLVLGIFVGSFIAAKASREFRVRAADAQTTLRSGLGGVLMGFGASIAG 300
Query 301 GCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASWLVYVRPQRKARLATAAAN 352
GCSIGNGLVMTAMMTWQGW+GLVFMILGVWTASWLVYVRPQRKARLATAAAN
Sbjct 301 GCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN 352
>P31064 Probable transporter YedE [Escherichia coli (strain K12)]
Length=401
Score = 86.3 bits (212), Expect = 1e-22
Identities = 43/102 (42%), Positives = 61/102 (60%), Gaps = 2/102 (2%)
Query 9 LICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAISVQSVGVFALIQAGLLTYE 68
++ G G +++R + C T FRDM+I M A++I ++V ++G+F+ +Q G+
Sbjct 225 MLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYVQLGVEPKI 284
Query 69 AGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSW 110
A P VIGG LFG GIVLAGGC TG YRA EG + W
Sbjct 285 MWAGP--NAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYW 324
Score = 42.4 bits (98), Expect = 3e-08
Identities = 20/78 (26%), Positives = 38/78 (49%), Gaps = 1/78 (1%)
Query 254 LVLGIFVGAFIAAKASREFRVRAADAQTTVRSGL-GGVLMGFGASIAGGCSIGNGLVMTA 312
++LG+F G F AA + ++R ++ + + GG++ GFGA +A GC++
Sbjct 83 MILGMFGGCFAAALWANNVKLRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIP 142
Query 313 MMTWQGWVGLVFMILGVW 330
+ W + +G W
Sbjct 143 QFSLHAWFFAIATAIGSW 160
Score = 35.8 bits (81), Expect = 3e-06
Identities = 61/258 (24%), Positives = 89/258 (34%), Gaps = 58/258 (22%)
Query 78 VIGGYLFGLGIVLAGGCA-----TG-------TWYRAGEGLIGSWI-ALFTYMVMSAVMR 124
+IGG + G G LA GC TG W+ A IGSW A FT + + R
Sbjct 116 IIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSWFGARFTLL---PIFR 172
Query 125 SPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVLLVITLWVVMKELKKPKLKVATLPP 184
P T + + F L + +L W ++ + +PKL +A L
Sbjct 173 IPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLG----WALLTAMNQPKLGLAMLFG 228
Query 185 RRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGITSPTANILQFLVAGD 244
G+ L E+ F +A + IT T ++
Sbjct 229 VGFGL---LIERAQICFTSAFR-------------------DMWITGRTHMAKAIIIGMA 266
Query 245 VKYINWGVFLVLGIFVGAFIAAKASREFRVRAADAQTTVRSGLGGVLMGFGASIAGGCSI 304
V I ++ LG+ E ++ A + GG+L GFG +AGGC
Sbjct 267 VSAIGIFSYVQLGV------------EPKIMWAGPNAVI----GGLLFGFGIVLAGGCET 310
Query 305 GNGLVMTAMMTWQGWVGL 322
G WVGL
Sbjct 311 GWMYRAVEGQVHYWWVGL 328
Score = 17.3 bits (33), Expect = 2.0
Identities = 12/37 (32%), Positives = 17/37 (46%), Gaps = 3/37 (8%)
Query 7 SGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFY 43
+ +I G L GF + C TG MY A ++ Y
Sbjct 290 NAVIGGLLFGFGIVLAGGCETGW---MYRAVEGQVHY 323
Score = 15.4 bits (28), Expect = 8.2
Identities = 20/68 (29%), Positives = 29/68 (43%), Gaps = 10/68 (15%)
Query 57 FALIQAGLLTYEAGAFPWLG-TVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIALFT 115
F ++ LLT A P LG ++ G FGL I A C T + WI T
Sbjct 205 FGMLGWALLT--AMNQPKLGLAMLFGVGFGLLIERAQICFTSAFR-------DMWITGRT 255
Query 116 YMVMSAVM 123
+M + ++
Sbjct 256 HMAKAIII 263
>Q06400 Probable transporter YedE [Salmonella typhimurium (strain
LT2 / SGSC1412 / ATCC 700720)]
Length=401
Score = 84.7 bits (208), Expect = 3e-22
Identities = 42/104 (40%), Positives = 62/104 (60%), Gaps = 6/104 (6%)
Query 9 LICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAISVQSVGVFALIQAGLLTYE 68
++ G G +++R + C T FRD++I+ M A++ ++V ++G+F+ +Q G+
Sbjct 225 MLFGVGFGLLIERAQICFTSAFRDLWISGRAHMAKAIIFGMAVSAIGIFSYVQLGV---- 280
Query 69 AGAFPWLG--TVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSW 110
A W G VIGG LFG GIVLAGGC TG YRA EG + W
Sbjct 281 APKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYW 324
Score = 40.8 bits (94), Expect = 8e-08
Identities = 21/78 (27%), Positives = 39/78 (50%), Gaps = 1/78 (1%)
Query 254 LVLGIFVGAFIAAKASREFRVRAADAQTT-VRSGLGGVLMGFGASIAGGCSIGNGLVMTA 312
++LG+F G F AA + ++R ++ V++ GG++ GFGA +A GC++
Sbjct 83 MILGMFGGCFAAALWANNVKLRMPRSRIRIVQAVAGGIIAGFGARLAMGCNLAAFFTGIP 142
Query 313 MMTWQGWVGLVFMILGVW 330
+ W + +G W
Sbjct 143 QFSLHAWFFALATAIGSW 160
Score = 36.2 bits (82), Expect = 2e-06
Identities = 58/258 (22%), Positives = 89/258 (34%), Gaps = 58/258 (22%)
Query 78 VIGGYLFGLGIVLAGGCA-----TG-------TWYRAGEGLIGSWI-ALFTYMVMSAVMR 124
V GG + G G LA GC TG W+ A IGSW A FT + + R
Sbjct 116 VAGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFALATAIGSWFGARFTLL---PIFR 172
Query 125 SPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVLLVITLWVVMKELKKPKLKVATLPP 184
P T + + F L + + ++ W ++ + +PKL +A L
Sbjct 173 IPVKMQKVSAASPLTQKPDQARRRFRLGMLVFIGMIG----WALLTAMHQPKLGLAML-- 226
Query 185 RRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGITSPTANILQFLVAGD 244
G+ L +R T+ L ++ A +FG+ +++ I ++ G
Sbjct 227 --FGVGFGLLIERAQICFTSAFRDL--WISGRAHMAKAIIFGMAVSA--IGIFSYVQLGV 280
Query 245 VKYINWGVFLVLGIFVGAFIAAKASREFRVRAADAQTTVRSGLGGVLMGFGASIAGGCSI 304
I W + +GG+L GFG +AGGC
Sbjct 281 APKIMWA------------------------------GPNAVIGGLLFGFGIVLAGGCET 310
Query 305 GNGLVMTAMMTWQGWVGL 322
G WVGL
Sbjct 311 GWMYRAVEGQVHYWWVGL 328
Score = 17.3 bits (33), Expect = 2.0
Identities = 12/37 (32%), Positives = 17/37 (46%), Gaps = 3/37 (8%)
Query 7 SGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFY 43
+ +I G L GF + C TG MY A ++ Y
Sbjct 290 NAVIGGLLFGFGIVLAGGCETGW---MYRAVEGQVHY 323
Lambda K H a alpha
0.330 0.142 0.454 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 329588
Database: 496abc7fa76d5f65db9947beca56c571.SwissProt.fasta
Posted date: May 3, 2024 12:21 AM
Number of letters in database: 1,154
Number of sequences in database: 3
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
