BLASTP 2.12.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.


Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.



Database: f84ba6deedbdb255d6eae2f668a31c14.SwissProt.fasta
           5 sequences; 1,833 total letters



Query= Z2279

Length=382
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

P76108 Bifunctional polyhydroxybutyrate synthase / ABC transporte...  790     0.0  
Q9I0P5 Putrescine/agmatine-binding protein [Pseudomonas aeruginos...  92.4    1e-24
Q9I6J0 Spermidine-binding periplasmic protein SpuE [Pseudomonas a...  90.5    6e-24
P31133 Putrescine-binding periplasmic protein PotF [Escherichia c...  81.3    9e-21
P44731 Spermidine/putrescine-binding periplasmic protein 2 [Haemo...  63.5    6e-15


>P76108 Bifunctional polyhydroxybutyrate synthase / ABC transporter 
periplasmic binding protein [Escherichia coli (strain 
K12)]
Length=381

 Score = 790 bits (2040),  Expect = 0.0
 Identities = 377/381 (99%), Positives = 377/381 (99%), Gaps = 0/381 (0%)

Query  1    MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGYIERGQTDKQYDWVTQF  60
            MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGYIERGQTDKQYDWVTQF
Sbjct  1    MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGYIERGQTDKQYDWVTQF  60

Query  61   EKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLIMGKRVQPINTALIPNWKT  120
            EKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLIMGKRVQPINTALIPNWKT
Sbjct  61   EKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLIMGKRVQPINTALIPNWKT  120

Query  121  LDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPPDSWQVVFVEQNLQDGKSNKG  180
            LDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPPDSWQVVFVEQNL DGKSNKG
Sbjct  121  LDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPPDSWQVVFVEQNLPDGKSNKG  180

Query  181  RVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQYQAVLKVLRDQHSLIHRYWHDTT  240
            RVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQYQAVLKVLR QHSLIHRYWHDTT
Sbjct  181  RVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQYQAVLKVLRAQHSLIHRYWHDTT  240

Query  241  VQMSDFKNEGVVASSAWPYQTNALKAEGQPVATVFPKEGVTGWADTTMLHSEAKHPVCAY  300
            VQMSDFKNEGVVASSAWPYQ NALKAEGQPVATVFPKEGVTGWADTTMLHSEAKHPVCAY
Sbjct  241  VQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFPKEGVTGWADTTMLHSEAKHPVCAY  300

Query  301  KWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSLLGEKGCETNGFNYFDKIAFWKTPIAE  360
            KWMNWSLTPKVQGDVAAWFGSLPVVPEGCKAS LLGEKGCETNGFNYFDKIAFWKTPIAE
Sbjct  301  KWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPLLGEKGCETNGFNYFDKIAFWKTPIAE  360

Query  361  GGKFVPYSRWTQDYIAIMGGR  381
            GGKFVPYSRWTQDYIAIMGGR
Sbjct  361  GGKFVPYSRWTQDYIAIMGGR  381


>Q9I0P5 Putrescine/agmatine-binding protein [Pseudomonas aeruginosa 
(strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 
/ LMG 12228 / 1C / PRS 101 / PAO1)]
Length=367

 Score = 92.4 bits (228),  Expect = 1e-24
 Identities = 90/355 (25%), Positives = 149/355 (42%), Gaps = 36/355 (10%)

Query  10   LCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGYIERGQTDKQYDWVTQFEKETGCAVN  69
            +CAL+++I+T   A    +    +  + +  W  +I   +T K       F+KETG  V 
Sbjct  4    VCALALSILTTIGATAADSAWAAQTSVHLYNWYDFIAP-ETPKA------FQKETGTRVV  56

Query  70   VKT--AATSDEMVSLMTKGGYDLVTASGDASLRLIMGKRVQPINTALIPNWKTLDPRVVK  127
            + T  +A + +   ++ + GYD+V  + +    LI    +Q ++   +P+WK LD  ++ 
Sbjct  57   LDTFDSAETAQGKLMVGRSGYDVVVITSNILPGLIKAGVLQELDRDRLPHWKNLDADILG  116

Query  128  GDWFNVGGKVYGTPYQWGPNLLMYNTKTF------PTPPDSWQVVFVEQNLQDGKSNKGR  181
                N  G  Y  PY WG   + Y+            P DSW +VF E+N+   + ++  
Sbjct  117  KLQANDPGNRYAVPYLWGTTGIAYDVDKVRKLLGPDAPVDSWDLVFKEENI--SRLSQCG  174

Query  182  VQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQYQAVLKVLRDQHSLIHRYWHDTTV  241
            V   D    +   AL        LG+    Q   E YQ   ++L      I RY+  + V
Sbjct  175  VATLDSSTELVSIAL------NYLGLPHNSQ-NPEDYQKAQELLLKVRPYI-RYFDSSRV  226

Query  242  QMSDFKNEGVVASSAW------PYQTNALKAEGQPVATVFPKEGVTGWADTTMLHSEAKH  295
              +D  N  V     W          N     G+ +A   P+EG   WA+  +L  +A H
Sbjct  227  D-TDLSNGNVCVVVGWQGTAYMAQVNNEQAGNGRHIAYSIPREGSLVWAENMVLLKDAPH  285

Query  296  PVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSLLGEKGCETNGFNYFDK  350
            P   Y  +++ L P+V    + + G     P G +A+  L E+    N   Y  K
Sbjct  286  PQQGYALIDYLLRPEVIARTSNYVG----YPNGNQAALPLVERKLRENPAVYLSK  336


>Q9I6J0 Spermidine-binding periplasmic protein SpuE [Pseudomonas 
aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / 
JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)]
Length=365

 Score = 90.5 bits (223),  Expect = 6e-24
 Identities = 85/322 (26%), Positives = 133/322 (41%), Gaps = 37/322 (11%)

Query  20   AHAAEPPTNLDKPEGRLDIIAWPGYIERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEM  79
            A A   P   +K    L I  W  YI           +  F KE+G  V+     +++ +
Sbjct  15   ATAIAGPVQAEKKS--LHIYNWTDYIAPTT-------LKDFTKESGIDVSYDVFDSNETL  65

Query  80   VSLMTKG--GYDLVTASGDASLRLIMGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKV  137
               +  G  GYD+V  S +   + I     Q ++ + +PNWK LDP ++K    +  G  
Sbjct  66   EGKLVSGHSGYDIVVPSNNFLGKQIQAGAFQKLDKSKLPNWKNLDPALLKQLEVSDPGNQ  125

Query  138  YGTPYQWGPNLLMYNTKTF-----PTPPDSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIA  192
            Y  PY WG N + YN           P DSW ++F  +N++  K  K  V   D    + 
Sbjct  126  YAVPYLWGTNGIGYNVAKVKEVLGDQPIDSWAILFEPENMK--KLAKCGVAFMDSGDEML  183

Query  193  DAALFVKATQPQLGISDPYQLTEEQYQAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVV  252
             AAL      P       Y+  EE    VL  +R   S  H     ++  +SD  N  + 
Sbjct  184  PAALNYLGLDPNTHDPKDYKKAEE----VLTKVRPYVSYFH-----SSKYISDLANGNIC  234

Query  253  ASSAWPYQTNALKA--------EGQPVATVFPKEGVTGWADTTMLHSEAKHPVCAYKWMN  304
               A+ Y  +  +A        +G  +  V PKEG   W D   + ++AK    AY +++
Sbjct  235  V--AFGYSGDVFQAAARAEEAGKGIDIQYVIPKEGANLWFDLMAIPADAKAADNAYAFID  292

Query  305  WSLTPKVQGDVAAWFGSLPVVP  326
            + L P+V   V+ + G    +P
Sbjct  293  YLLRPEVIAKVSDYVGYANAIP  314


>P31133 Putrescine-binding periplasmic protein PotF [Escherichia 
coli (strain K12)]
Length=370

 Score = 81.3 bits (199),  Expect = 9e-21
 Identities = 77/295 (26%), Positives = 122/295 (41%), Gaps = 31/295 (11%)

Query  36   LDIIAWPGYIERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKG--GYDLVTA  93
            L I  W  YI         D V  FEKETG  V      +++ +   +  G  G+DLV  
Sbjct  32   LHIYNWSDYIAP-------DTVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVP  84

Query  94   SGDASLRLIMGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNT  153
            S     R +     QP++ + +P WK LDP ++K    +     +  PY W    + YN 
Sbjct  85   SASFLERQLTAGVFQPLDKSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNV  144

Query  154  KTFP------TPPDSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGI  207
                       P DSW ++   +NL+  KS    V   D P  +    L      P    
Sbjct  145  DKVKAVLGENAPVDSWDLILKPENLEKLKS--CGVSFLDAPEEVFATVLNYLGKDPNSTK  202

Query  208  SDPYQLTEEQYQAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVV-----ASSAWPYQTN  262
            +D Y  T      +LK+  +      RY+H ++  ++D  N  +      A   W     
Sbjct  203  ADDY--TGPATDLLLKLRPN-----IRYFH-SSQYINDLANGDICVAIGWAGDVWQASNR  254

Query  263  ALKAE-GQPVATVFPKEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVA  316
            A +A+ G  V+   PKEG   + D   + ++AK+   AY+++N+ L P V   ++
Sbjct  255  AKEAKNGVNVSFSIPKEGAMAFFDVFAMPADAKNKDEAYQFLNYLLRPDVVAHIS  309


>P44731 Spermidine/putrescine-binding periplasmic protein 2 [Haemophilus 
influenzae (strain ATCC 51907 / DSM 11121 / KW20 
/ Rd)]
Length=350

 Score = 63.5 bits (153),  Expect = 6e-15
 Identities = 76/318 (24%), Positives = 126/318 (40%), Gaps = 44/318 (14%)

Query  3    KTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGYIERGQTDKQYDWVTQFEK  62
            K F   SL  L ++     AA    N      +L +  W  Y+         D V QF K
Sbjct  2    KKFFAHSLKNLFLSTTALFAASAFAN-----NKLYVYNWTDYVPS-------DLVAQFSK  49

Query  63   ETGCAVNVKTAATSDEMVSLM-----TKGGYDLVTASGDASLRLIMGKRVQPINTALIPN  117
            ETG  V   T  +++EM + +     T  GYDLV  S     ++I  K +QPI+ + + N
Sbjct  50   ETGIEVIYSTFESNEEMYAKLKLTQNTGSGYDLVFPSSYYVNKMIKEKMLQPIDQSKLTN  109

Query  118  WKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNT-----KTFPTPPDSWQVVFVEQNL  172
               + P+ +    F+   K Y  PY +G   +  N      KT  +  D W+  F     
Sbjct  110  IHQI-PKHLLNKEFDPENK-YSLPYVYGLTGIEVNADEIDPKTITSWADLWKPEF-----  162

Query  173  QDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQYQAVLKVLRDQHSLI  232
                  KG+V        +   AL +    P     +  +   E+ + +L  +   +S  
Sbjct  163  ------KGKVLMTSDAREVFHVALLLDGKSPNTTNEEDIKTAYERLEKLLPNVATFNS--  214

Query  233  HRYWHDTTVQMSDFKNEGVVASSAWPYQTNALKAEGQPVATVFPKEGVTGWADTTMLHSE  292
                   + ++   + E V     W       + E Q +  V+PKEG   W D   + + 
Sbjct  215  ------DSPEVPYVQGE-VAIGMIWNGSAYLAQKENQSLQFVYPKEGAIFWMDNYAIPTT  267

Query  293  AKHPVCAYKWMNWSLTPK  310
            AK+   A+K++++ L P+
Sbjct  268  AKNVEGAHKFIDFLLRPE  285



Lambda      K        H        a         alpha
   0.318    0.134    0.429    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 555148


  Database: f84ba6deedbdb255d6eae2f668a31c14.SwissProt.fasta
    Posted date:  May 31, 2024  11:37 AM
  Number of letters in database: 1,833
  Number of sequences in database:  5



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40