BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 56cbdfd8648a485bac25920651d84db7.SwissProt.fasta
4 sequences; 1,682 total letters
Query= Z0746
Length=407
Score E
Sequences producing significant alignments: (Bits) Value
P77216 Uncharacterized protein YbdN [Escherichia coli (strain K12)] 875 0.0
Q58383 Uncharacterized protein MJ0973 [Methanocaldococcus jannasc... 40.0 3e-07
Q5P2I9 tRNA(Ile)-lysidine synthase [Aromatoleum aromaticum (strai... 37.4 2e-06
Q9HXZ3 tRNA(Ile)-lysidine synthase [Pseudomonas aeruginosa (strai... 36.2 4e-06
>P77216 Uncharacterized protein YbdN [Escherichia coli (strain
K12)]
Length=406
Score = 875 bits (2261), Expect = 0.0
Identities = 405/406 (99%), Positives = 406/406 (100%), Gaps = 0/406 (0%)
Query 1 MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLMLHLTAELARQMGKKICV 60
MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLMLHLTAELARQMGKKICV
Sbjct 1 MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLMLHLTAELARQMGKKICV 60
Query 61 LFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNSLSQYQPEWQCWEPDVEWV 120
LFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNSLSQYQPEWQCWEPDVEWV
Sbjct 61 LFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNSLSQYQPEWQCWEPDVEWV 120
Query 121 RQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPAAMMIGIRADESYNRFVAIAS 180
RQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPAAMMIGIRADESYNRFVAIAS
Sbjct 121 RQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPAAMMIGIRADESYNRFVAIAS 180
Query 181 LNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTWYANHQSLCNPLYNLMYQAGVPL 240
LNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTWYANHQSLCNPLYNLMYQAGVPL
Sbjct 181 LNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTWYANHQSLCNPLYNLMYQAGVPL 240
Query 241 RHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVSGVKSGGIYAGHDNHFYGHRKILKP 300
RHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVSGVKSGGIYAGHDNHFYGHRKILKP
Sbjct 241 RHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVSGVKSGGIYAGHDNHFYGHRKILKP 300
Query 301 EHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHWYQKKGIEVPQTQQGDIGAKDIPSWRR 360
EHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHWYQKKGIEVPQTQQGDIGAKDIPSWRR
Sbjct 301 EHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHWYQKKGIEVPQTQQGDIGAKDIPSWRR 360
Query 361 ICKVLLNNDYWCRALSFSPTKAKNYQRYNERIKGKRQEWGILCNND 406
ICKVLLNNDYWCRALSFSPTK+KNYQRYNERIKGKRQEWGILCNND
Sbjct 361 ICKVLLNNDYWCRALSFSPTKSKNYQRYNERIKGKRQEWGILCNND 406
>Q58383 Uncharacterized protein MJ0973 [Methanocaldococcus jannaschii
(strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 /
NBRC 100440)]
Length=411
Score = 40.0 bits (92), Expect = 3e-07
Identities = 44/199 (22%), Positives = 77/199 (39%), Gaps = 45/199 (23%)
Query 33 SFSGGKDSGLMLHLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYW 92
SFSGGKDS + + LA ++ + V+FID +F TI++V+ + Y
Sbjct 184 SFSGGKDSSV----STLLANKVIDDLEVIFIDTGLEFKDTIDFVKKFAKKYD-------- 231
Query 93 VALPLTTQNSLSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAE 152
+ L + + +Y + D W C +P E
Sbjct 232 LNLVVLKGKNFWEYLEKEGIPTKDYRWCNS-----------VCKLEP----------LKE 270
Query 153 WFSQKRPAAMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKV 212
+ + + + G R ES+ R + K F +++ I+PI DW+
Sbjct 271 YLKKYKRVYTIDGSRRYESFTR-EKLTYERKSGFIENQI-----------NIFPILDWRG 318
Query 213 ADIWTWYANHQSLCNPLYN 231
D+W+W + + N LY+
Sbjct 319 TDVWSWIYLNDVIYNELYD 337
>Q5P2I9 tRNA(Ile)-lysidine synthase [Aromatoleum aromaticum (strain
EbN1)]
Length=423
Score = 37.4 bits (85), Expect = 2e-06
Identities = 16/35 (46%), Positives = 22/35 (63%), Gaps = 0/35 (0%)
Query 29 RVCVSFSGGKDSGLMLHLTAELARQMGKKICVLFI 63
R+CV+FSGG DS +LHL A L + G +C +
Sbjct 12 RLCVAFSGGADSTTLLHLLARLRPRFGFDLCAAHV 46
Score = 19.2 bits (38), Expect = 0.88
Identities = 7/18 (39%), Positives = 10/18 (56%), Gaps = 2/18 (11%)
Query 259 LYHVIEP--DRWAAMCAR 274
++H + P D W CAR
Sbjct 46 VHHALSPNADAWLDFCAR 63
>Q9HXZ3 tRNA(Ile)-lysidine synthase [Pseudomonas aeruginosa (strain
ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG
12228 / 1C / PRS 101 / PAO1)]
Length=442
Score = 36.2 bits (82), Expect = 4e-06
Identities = 22/50 (44%), Positives = 29/50 (58%), Gaps = 11/50 (22%)
Query 6 IPLPLNILEAA---RERITWTLNTLPRVCVSFSGGKDSGLMLHLTAELAR 52
+PL +L+A RE W CV+FSGG DS ++LHL A+LAR
Sbjct 4 VPLESRLLQALAPWREAGGW--------CVAFSGGLDSTVLLHLLAQLAR 45
Score = 19.6 bits (39), Expect = 0.67
Identities = 10/28 (36%), Positives = 15/28 (54%), Gaps = 7/28 (25%)
Query 218 WYANHQSLCNPLYNLMYQAGVPLRHMRI 245
W A+ Q++C L G+PLR R+
Sbjct 68 WPAHCQAVCRSL-------GIPLRVERV 88
Score = 16.9 bits (32), Expect = 4.6
Identities = 7/11 (64%), Positives = 8/11 (73%), Gaps = 0/11 (0%)
Query 9 PLNILEAARER 19
PL L AAR+R
Sbjct 262 PLRRLSAARQR 272
Lambda K H a alpha
0.324 0.138 0.472 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 555588
Database: 56cbdfd8648a485bac25920651d84db7.SwissProt.fasta
Posted date: Jun 1, 2024 1:09 PM
Number of letters in database: 1,682
Number of sequences in database: 4
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
