BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 354e87f878cb9fee5c1987c75f665d4d.SwissProt.fasta
12 sequences; 2,496 total letters
Query= Z1993
Length=232
Score E
Sequences producing significant alignments: (Bits) Value
P39163 Glutathione-specific gamma-glutamylcyclotransferase [Esche... 469 1e-174
Q84QC1 Gamma-glutamylcyclotransferase 2-3 [Arabidopsis thaliana] 89.7 9e-26
B3STU3 Glutathione-specific gamma-glutamylcyclotransferase 1 [Rat... 89.0 3e-25
Q8R3J5 Glutathione-specific gamma-glutamylcyclotransferase 1 [Mus... 88.6 4e-25
Q9BUX1 Glutathione-specific gamma-glutamylcyclotransferase 1 [Hom... 84.3 1e-23
Q641Z5 Putative glutathione-specific gamma-glutamylcyclotransfera... 83.2 2e-23
Q9CQG1 Putative glutathione-specific gamma-glutamylcyclotransfera... 82.8 2e-23
Q5PPV4 Putative glutathione-specific gamma-glutamylcyclotransfera... 77.8 2e-21
Q84MC1 Gamma-glutamylcyclotransferase 2-2 [Arabidopsis thaliana] 78.6 2e-21
Q8GY54 Gamma-glutamylcyclotransferase 2-1 [Arabidopsis thaliana] 78.2 3e-21
Q8WUX2 Glutathione-specific gamma-glutamylcyclotransferase 2 [Hom... 74.7 3e-20
P32656 Glutathione-specific gamma-glutamylcyclotransferase [Sacch... 48.1 2e-10
>P39163 Glutathione-specific gamma-glutamylcyclotransferase [Escherichia
coli (strain K12)]
Length=231
Score = 469 bits (1207), Expect = 1e-174
Identities = 228/231 (99%), Positives = 230/231 (99%), Gaps = 0/231 (0%)
Query 1 VITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDERPVWIFGYGSLMWNPA 60
+ITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE PVWIFGYGSLMWNPA
Sbjct 1 MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDEGPVWIFGYGSLMWNPA 60
Query 61 LEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGGRTTGVAYRLPEETLEQEL 120
LEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGGRTTGVAYRLPEETLEQEL
Sbjct 61 LEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGGRTTGVAYRLPEETLEQEL 120
Query 121 TLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHPEYESDTRAQVIAPLIAAASG 180
TLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHPEYESDTRAQVIAPLIAAASG
Sbjct 121 TLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHPEYESDTRAQVIAPLIAAASG 180
Query 181 PLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLAENYPDGVLRPGFA 231
PLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLAEN+PDGVLRPGFA
Sbjct 181 PLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLAENFPDGVLRPGFA 231
>Q84QC1 Gamma-glutamylcyclotransferase 2-3 [Arabidopsis thaliana]
Length=199
Score = 89.7 bits (221), Expect = 9e-26
Identities = 57/182 (31%), Positives = 90/182 (49%), Gaps = 8/182 (4%)
Query 47 VWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGGRTT- 105
+W+FGYGSL+W F ES G + G+ R F T RGT PGR + L+
Sbjct 3 MWVFGYGSLIWKTGFPFDESLPGFIKGYRRVFHQGSTDHRGTPDFPGRTVTLEAAHEEVC 62
Query 106 -GVAYRLPEETLEQELTL---LWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDP---R 158
GVAY++ +E +++ L + +++ YL + + + +V+I P
Sbjct 63 CGVAYKITKEEDKRDALLHLEVREKQYDQKEYLDFFTDSNASEPAVAGVMVYIASPDKKS 122
Query 159 HPEYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA 218
+ Y + IA I A GP G N YLF+LE+ L +LG +D + DL V+ +L+
Sbjct 123 NNNYLGPAPLEDIAKQIVKAKGPSGPNRDYLFNLEEALAQLGFKDKHVTDLANQVRHILS 182
Query 219 EN 220
E+
Sbjct 183 ES 184
>B3STU3 Glutathione-specific gamma-glutamylcyclotransferase 1
[Rattus norvegicus]
Length=222
Score = 89.0 bits (219), Expect = 3e-25
Identities = 63/186 (34%), Positives = 95/186 (51%), Gaps = 8/186 (4%)
Query 43 DERPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEG- 101
D + +WIFGYGSL+W P +++S G + G+ R F T RG+ PGR++ L E
Sbjct 29 DPQALWIFGYGSLVWKPDFAYSDSRVGFVRGYSRRFWQGDTFHRGSDKMPGRVVTLLEDH 88
Query 102 -GRTTGVAYRLPEETLEQELTLLWKREMITGCY---LPTWCQLDLDDGRTVNAIVFIMDP 157
G T GVAY++ E + + L L RE + G Y T+ D D + + A+ ++ P
Sbjct 89 EGCTWGVAYQVRGEQVSEALKYLNVREAVLGGYDTKEVTFYPQDTPD-QPLTALAYVATP 147
Query 158 RHPEYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLG--MQDDGLNDLLVSVKK 215
++P Y +VIA I A G G N +YL L + G QD+ L ++ +V
Sbjct 148 QNPGYLGPAPEEVIATQILACRGFSGHNLEYLLRLADFMQLCGPQAQDEHLEAIVDAVGS 207
Query 216 LLAENY 221
LL +Y
Sbjct 208 LLPCSY 213
>Q8R3J5 Glutathione-specific gamma-glutamylcyclotransferase 1
[Mus musculus]
Length=223
Score = 88.6 bits (218), Expect = 4e-25
Identities = 63/186 (34%), Positives = 95/186 (51%), Gaps = 8/186 (4%)
Query 43 DERPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEG- 101
D + +WIFGYGSL+W P +++S G + G+ R F T RG+ PGR++ L E
Sbjct 30 DPQALWIFGYGSLVWKPDFAYSDSRVGFVRGYSRRFWQGDTFHRGSDKMPGRVVTLLEDR 89
Query 102 -GRTTGVAYRLPEETLEQELTLLWKREMITGCY---LPTWCQLDLDDGRTVNAIVFIMDP 157
G T GVAY++ E + + L L RE + G Y T+ D D + + A+ ++ P
Sbjct 90 EGCTWGVAYQVRGEQVNEALKYLNVREAVLGGYDTKEVTFYPQDTPD-QPLTALAYVATP 148
Query 158 RHPEYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLG--MQDDGLNDLLVSVKK 215
++P Y +VIA I A G G N +YL L + G QD+ L ++ +V
Sbjct 149 QNPGYLGPAPEEVIATQILACRGFSGHNLEYLLRLADFMQLCGPQAQDEHLEAIVDAVGT 208
Query 216 LLAENY 221
LL +Y
Sbjct 209 LLPCSY 214
>Q9BUX1 Glutathione-specific gamma-glutamylcyclotransferase 1
[Homo sapiens]
Length=222
Score = 84.3 bits (207), Expect = 1e-23
Identities = 60/182 (33%), Positives = 92/182 (51%), Gaps = 8/182 (4%)
Query 43 DERPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEG- 101
D + +WIFGYGSL+W P +++S G + G+ R F T RG+ PGR++ L E
Sbjct 29 DPQALWIFGYGSLVWRPDFAYSDSRVGFVRGYSRRFWQGDTFHRGSDKMPGRVVTLLEDH 88
Query 102 -GRTTGVAYRLPEETLEQELTLLWKREMITGCY---LPTWCQLDLDDGRTVNAIVFIMDP 157
G T GVAY++ E + + L L RE + G Y T+ D D + + A+ ++ P
Sbjct 89 EGCTWGVAYQVQGEQVSKALKYLNVREAVLGGYDTKEVTFYPQDAPD-QPLKALAYVATP 147
Query 158 RHPEYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLG--MQDDGLNDLLVSVKK 215
++P Y + IA I A G G N +YL L + G QD+ L ++ +V
Sbjct 148 QNPGYLGPAPEEAIATQILACRGFSGHNLEYLLRLADFMQLCGPQAQDEHLAAIVDAVGT 207
Query 216 LL 217
+L
Sbjct 208 ML 209
>Q641Z5 Putative glutathione-specific gamma-glutamylcyclotransferase
2 [Rattus norvegicus]
Length=178
Score = 83.2 bits (204), Expect = 2e-23
Identities = 60/177 (34%), Positives = 87/177 (49%), Gaps = 8/177 (5%)
Query 47 VWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKE--GGRT 104
+W+FGYGSL+W + + G + + R F T RG +PGR++ L E GG
Sbjct 1 MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPGGSV 60
Query 105 TGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRT--VNAIVFIMDPRHPEY 162
GVAY+LP E+ T L RE G Y T D T + +++I +P Y
Sbjct 61 WGVAYKLPVGKEEEVKTYLDFRE--KGGYRTTTVIFYPKDSTTKPFSVLLYIGTCDNPNY 118
Query 163 ESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLAE 219
+ IA I A+GP G N +YLF L + KL +D ++ L S++KL+ E
Sbjct 119 LGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSIRKLVPED--ADEHLFSLEKLVKE 173
>Q9CQG1 Putative glutathione-specific gamma-glutamylcyclotransferase
2 [Mus musculus]
Length=178
Score = 82.8 bits (203), Expect = 2e-23
Identities = 60/177 (34%), Positives = 87/177 (49%), Gaps = 8/177 (5%)
Query 47 VWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKE--GGRT 104
+W+FGYGSL+W + + G + + R F T RG +PGR++ L E GG
Sbjct 1 MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPGGSV 60
Query 105 TGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRT--VNAIVFIMDPRHPEY 162
GVAY+LP E+ T L RE G Y T D T + +++I +P Y
Sbjct 61 WGVAYKLPVGKEEEVKTYLDFRE--KGGYRTTTVIFYPKDSTTKPFSVLLYIGTCDNPNY 118
Query 163 ESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLAE 219
+ IA I A+GP G N +YLF L + KL +D ++ L S++KL+ E
Sbjct 119 LGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSVRKLVPED--ADEHLFSLEKLVKE 173
>Q5PPV4 Putative glutathione-specific gamma-glutamylcyclotransferase
2 [Xenopus laevis]
Length=184
Score = 77.8 bits (190), Expect = 2e-21
Identities = 59/177 (33%), Positives = 85/177 (48%), Gaps = 8/177 (5%)
Query 47 VWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEG--GRT 104
+W+FGYGSL+W + E G ++ + R F T RG +PGR++ L E G
Sbjct 1 MWVFGYGSLIWKVDFPYVEKLVGYIMCYSRRFWQGSTDHRGVPGKPGRVVTLVEDPEGCV 60
Query 105 TGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDG--RTVNAIVFIMDPRHPEY 162
GVAYRLPE E+ L RE G Y + D + N +++I +P Y
Sbjct 61 WGVAYRLPEGKEEEVKAYLDFRE--KGGYRTSTVVFYPKDPSIQPFNVLLYIGTCDNPNY 118
Query 163 ESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLAE 219
+ IA I A GP G N +YLF L L L +D ++ L S++KL+ +
Sbjct 119 LGPAPLEDIAEQILNAVGPSGRNTEYLFELANSLRNLVPED--ADEHLFSLEKLVRQ 173
>Q84MC1 Gamma-glutamylcyclotransferase 2-2 [Arabidopsis thaliana]
Length=227
Score = 78.6 bits (192), Expect = 2e-21
Identities = 59/193 (31%), Positives = 84/193 (44%), Gaps = 29/193 (15%)
Query 47 VWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGGRT-- 104
+W+FGYGSL+WNP + E G + G+ R F L RGT P R L++
Sbjct 3 MWVFGYGSLVWNPGFHYDEKVLGFIKGYKRVFDLACIDHRGTPEHPARTCTLEKAEEAIC 62
Query 105 --TGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDL---------DD---GRTVNA 150
T R E + L +RE C+ DL DD
Sbjct 63 WGTAFCVRGGPEKERLAMEYLERRE----------CEYDLKTSVDFYKEDDPLKPAVTGV 112
Query 151 IVFIMDP---RHPEYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLN 207
IVF P + Y + +A IA A+GP G N YLF LE+ + +G ++D +
Sbjct 113 IVFTSTPDKVSNKYYLGPAPLEDMARQIATANGPCGNNRDYLFLLEKAMHDIGHEEDYVI 172
Query 208 DLLVSVKKLLAEN 220
+L V+K+LAE+
Sbjct 173 ELANEVRKVLAES 185
>Q8GY54 Gamma-glutamylcyclotransferase 2-1 [Arabidopsis thaliana]
Length=216
Score = 78.2 bits (191), Expect = 3e-21
Identities = 56/180 (31%), Positives = 83/180 (46%), Gaps = 9/180 (5%)
Query 47 VWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKE--GGRT 104
+W+FGYGSL+WNP +F E G + + R F L RGT P R L++ G
Sbjct 3 LWVFGYGSLIWNPGFDFDEKLIGYIKDYKRVFDLACIDHRGTPEHPARTCTLEQSTGAIC 62
Query 105 TGVAYRL---PE-ETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDP--- 157
G AY + PE E L E + E + + + + D IVF P
Sbjct 63 WGAAYCVRGGPEKEKLAMEYLERRECEYDSKTLVEFYTENDTSTPIVTGVIVFTSTPDKV 122
Query 158 RHPEYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLL 217
+ Y + +A IA ASGP G N +YLF LE+ + + +++ + +L V+K L
Sbjct 123 SNKYYLGPAPLEEMARQIATASGPCGNNREYLFKLEKAMFDIEHEEEYVIELANEVRKQL 182
>Q8WUX2 Glutathione-specific gamma-glutamylcyclotransferase 2
[Homo sapiens]
Length=184
Score = 74.7 bits (182), Expect = 3e-20
Identities = 56/177 (32%), Positives = 85/177 (48%), Gaps = 8/177 (5%)
Query 47 VWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKE--GGRT 104
+W+FGYGSL+W + + G + + R F T RG +PGR++ L E G
Sbjct 1 MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPAGCV 60
Query 105 TGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRT--VNAIVFIMDPRHPEY 162
GVAYRLP E+ L RE G Y T D T + +++I +P+Y
Sbjct 61 WGVAYRLPVGKEEEVKAYLDFRE--KGGYRTTTVIFYPKDPTTKPFSVLLYIGTCDNPDY 118
Query 163 ESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLAE 219
+ IA I A+GP G N +YLF L + L ++ ++ L +++KL+ E
Sbjct 119 LGPAPLEDIAEQIFNAAGPSGRNTEYLFELANSIRNLVPEE--ADEHLFALEKLVKE 173
>P32656 Glutathione-specific gamma-glutamylcyclotransferase [Saccharomyces
cerevisiae (strain ATCC 204508 / S288c)]
Length=232
Score = 48.1 bits (113), Expect = 2e-10
Identities = 53/227 (23%), Positives = 82/227 (36%), Gaps = 54/227 (24%)
Query 43 DERPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLAL---- 98
D +W+ GYGSL++ P +T + G+ R F T RGT PGR+ L
Sbjct 4 DNSGIWVLGYGSLIYKPPSHYTHRIPAIIHGFARRFWQSSTDHRGTPANPGRVATLIPYE 63
Query 99 -----------------------KEGGRTTGVAYRLPEETLEQELTLLWKREM------I 129
+ T GV Y +P E ++ L RE
Sbjct 64 DIIRQTAFLKNVNLYSESAPIQDPDDLVTIGVVYYIPPEHAQEVREYLNVREQNGYTLHE 123
Query 130 TGCYLPTWCQLDLDDGRTVNAI--------------VFIMDPRHPEYESDTRAQVIAPLI 175
+L T + + + G + + V+I + + A +I
Sbjct 124 VEVHLETNREHEAELGEALEQLPRHNKSGKRVLLTSVYIGTIDNEAFVGPETVDETAKVI 183
Query 176 AAASGPLGTNAQYLFSLEQELIKLGMQ-------DDGLNDLLVSVKK 215
A + GP G+N +YL LEQ L ++ + D L LL +V K
Sbjct 184 AVSHGPSGSNYEYLAKLEQALAQMPIMKERGRITDHYLTALLETVNK 230
Lambda K H a alpha
0.324 0.139 0.439 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 395760
Database: 354e87f878cb9fee5c1987c75f665d4d.SwissProt.fasta
Posted date: May 31, 2024 8:37 AM
Number of letters in database: 2,496
Number of sequences in database: 12
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
