BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 11561f07322244c662a7c3a46e59d6e1.SwissProt.fasta
7 sequences; 1,978 total letters
Query= Z1394
Length=286
Score E
Sequences producing significant alignments: (Bits) Value
P19932 Hydrogenase-1 operon protein HyaF [Escherichia coli (strai... 577 0.0
P31911 Hydrogenase expression/formation protein HoxQ [Cupriavidus... 124 8e-38
P28150 Hydrogenase expression/formation protein HupH [Rhizobium l... 114 7e-34
P48340 Hydrogenase expression/formation protein HupH [Bradyrhizob... 112 3e-33
Q03007 Hydrogenase expression/formation protein HupH [Rhodobacter... 110 2e-32
Q43957 Hydrogenase expression/formation protein HupQ [Azotobacter... 109 6e-32
P40594 Hydrogenase expression/formation protein HoxQ [Azotobacter... 108 2e-31
>P19932 Hydrogenase-1 operon protein HyaF [Escherichia coli (strain
K12)]
Length=285
Score = 577 bits (1487), Expect = 0.0
Identities = 283/285 (99%), Positives = 284/285 (99%), Gaps = 0/285 (0%)
Query 1 MSETFFHLLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQL 60
MSETFFHLLGP TQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQL
Sbjct 1 MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQL 60
Query 61 SQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRV 120
S+RQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRV
Sbjct 61 SERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRV 120
Query 121 RRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPPIDGLMNGLPLAHELLAHVRN 180
RRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPPIDGLMNGLPLAHELLAHVRN
Sbjct 121 RRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPPIDGLMNGLPLAHELLAHVRN 180
Query 181 PDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT 240
PDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
Sbjct 181 PDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT 240
Query 241 LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEAAPT 285
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEAAPT
Sbjct 241 LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEAAPT 285
>P31911 Hydrogenase expression/formation protein HoxQ [Cupriavidus
necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB
10442 / H16 / Stanier 337)]
Length=282
Score = 124 bits (312), Expect = 8e-38
Identities = 88/274 (32%), Positives = 136/274 (50%), Gaps = 6/274 (2%)
Query 8 LLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQLSQRQPPL 67
LLGP +Q D++ +P V P + EQ A Q ++L + L + P
Sbjct 10 LLGPGSQAEDETLDYIHMPQGMSVYTPPVLPEPEQIACLTQAQSVLRAILDALDRADPKQ 69
Query 68 GEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRVRRRRGEK 127
++L L+ ++R + +LGEGEVS RI+ + + IQEA+F G+WRV +
Sbjct 70 PNP-RIELWALDEENRQLLKQVLGEGEVSARIE--GEYQVYIQEAVFAGVWRVVSTCPDA 126
Query 128 LLEDKLEAGCAPLALWQAATQNLLPTDSLLPPPIDGLMNGLPLAHELLAHVRN--PDAQP 185
D +E G AP L AA + DG+MN + E+ + + P
Sbjct 127 TT-DYIEVGAAPEVLRGAARYGANQWSLDVGRVPDGVMNAPSILAEIDDQLLSWHPGKPV 185
Query 186 HSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDTLKGPL 245
H +NLT LP+S D FL G ++ I + GYG I++T + + W + ++ +
Sbjct 186 HVVNLTLLPMSPQDITFLDETLGAASVTILSRGYGNCRISSTAVPYCWRVVYFNSQDTTI 245
Query 246 LESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWL 279
L + EI +PEV AAPEDL DS +RL+EV +W+
Sbjct 246 LNTVEITDLPEVACAAPEDLRDSHERLTEVLKWV 279
>P28150 Hydrogenase expression/formation protein HupH [Rhizobium
leguminosarum bv. viciae]
Length=282
Score = 114 bits (285), Expect = 7e-34
Identities = 84/272 (31%), Positives = 133/272 (49%), Gaps = 15/272 (6%)
Query 15 PNDDSFSMNPLPITCQ-------VNDEPSMAALEQCAHSPQVIALLNELQHQLS-QRQPP 66
P + +M +PI + +N + +A E + LL EL L+ Q+
Sbjct 9 PEGEGAAMTVMPIGAEPPLRARKLNFLATSSAEEMIRRCRRTAMLLPELADALAVQKAEQ 68
Query 67 LGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRVRRRRGE 126
G + D+ + DDR I LGEGEV+ A+ +++QEA+ G+WR+R +
Sbjct 69 PGRLF--DITDFPDDDRELITQTLGEGEVAGVAALANGVTAQMQEAVMAGVWRIRFTDVD 126
Query 127 -KLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPPIDGLMNGLPLAHELLAHV-RNPDAQ 184
+L+ D +E P A+ QA + LP P G MN +P+ E+ + R D
Sbjct 127 GRLIADYIEVASIPAAVRQACSG--LPASISHGPAPAGAMNVMPVLTEIGDRIARYRDGD 184
Query 185 P-HSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDTLKG 243
P H I + P+S D FL G G +Q+ + GYG + ATG R+VW ++ + +
Sbjct 185 PAHVITFSLFPMSPEDMAFLQDALGAGPVQLTSRGYGTCRVVATGARNVWSVQFYNAMDT 244
Query 244 PLLESYEICPIPEVVLAAPEDLVDSAQRLSEV 275
+L++ E+C IP V +AA ED DS RL ++
Sbjct 245 IILDTLEVCNIPSVAIAAEEDFRDSEARLRDI 276
>P48340 Hydrogenase expression/formation protein HupH [Bradyrhizobium
diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM
13628 / NBRC 14792 / USDA 110)]
Length=287
Score = 112 bits (281), Expect = 3e-33
Identities = 78/244 (32%), Positives = 125/244 (51%), Gaps = 6/244 (2%)
Query 36 SMAALEQCAHSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEV 95
++ + E P +ALL+ + ++ ++ L L NLN + I +LGEGEV
Sbjct 44 TLDSAELARSCPNAVALLSRIADAIAGQKADAPSQL-FRLANLNDLESKLIADVLGEGEV 102
Query 96 SVRIQQADDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDS 155
+ + D S ++IQE++ G+WRVR + LE G P + +AA +L D
Sbjct 103 AGVVALPDGSLAQIQESVLAGIWRVRLETDAN--HEYLEIGAVPEIVKRAAA-DLTSADF 159
Query 156 LLPPPIDGLMNGLPLAHELL--AHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQ 213
+ +G MN LP+ E+ A P + IN T LP+S D FL G IQ
Sbjct 160 EIGQVPEGAMNVLPVLAEIRERALAWRPGIRSQIINFTLLPMSPVDMSFLQDTIRNGPIQ 219
Query 214 IRTIGYGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLS 273
+ + GYG + +TG+R+VW ++ + + +L++ E+ +P V LAA ED DSA+RL
Sbjct 220 LVSRGYGTCRVLSTGIRNVWSVQFFNAMDTIILDTLEVGGVPTVALAADEDFEDSAERLK 279
Query 274 EVCQ 277
E+ +
Sbjct 280 EIIE 283
Score = 16.2 bits (30), Expect = 6.2
Identities = 4/13 (31%), Positives = 9/13 (69%), Gaps = 0/13 (0%)
Query 21 SMNPLPITCQVND 33
+MN LP+ ++ +
Sbjct 168 AMNVLPVLAEIRE 180
>Q03007 Hydrogenase expression/formation protein HupH [Rhodobacter
capsulatus]
Length=275
Score = 110 bits (274), Expect = 2e-32
Identities = 88/281 (31%), Positives = 132/281 (47%), Gaps = 22/281 (8%)
Query 6 FHL----LGPRTQPN-DDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQL 60
FHL GP +QP D+ LP + + A +P + LL+E+
Sbjct 5 FHLPPVGFGPGSQPEGDEELGYMQLPSGMRTYSAHLPEVEDSSAVTP-ALKLLDEIA--A 61
Query 61 SQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRV 120
G + + DL L+A +R I +G+GEV+++I+ +QE++F G+W V
Sbjct 62 GAEACARGGMASFDLAGLDAQNRALIAETMGQGEVAMKIRGIP--ALMVQESVFAGVWSV 119
Query 121 RRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPPIDGLMNGLPLAHELL----A 176
D++E G P A A + + LPP DG++N L EL A
Sbjct 120 AGAG-----VDRIEVGAVPAAALSRAFEPFRKGQTALPPLSDGVVNAPALIAELFDKSAA 174
Query 177 HVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLR 236
+V INLT LP +E D L + G G + I + GYG I AT HVW ++
Sbjct 175 YV---GGAADVINLTLLPHTEEDLTLLDYMLGEGAVTILSRGYGNCRITATATPHVWRVQ 231
Query 237 CTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQ 277
++ +L++ E+ +PEV LAA EDL DSA R+ EV +
Sbjct 232 FYNSTDALILDTIEVTTMPEVALAAREDLEDSAGRIREVLE 272
>Q43957 Hydrogenase expression/formation protein HupQ [Azotobacter
chroococcum mcd 1]
Length=284
Score = 109 bits (272), Expect = 6e-32
Identities = 83/273 (30%), Positives = 131/273 (48%), Gaps = 13/273 (5%)
Query 10 GPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQLSQRQPPLGE 69
GP + +D P+P + PS+ EQ P +ALL LQ L+ + +GE
Sbjct 13 GPGSHGEEDRLDYLPMPREMHTFERPSLPEPEQLDSHPIALALLERLQEALTAYR--IGE 70
Query 70 VLAVDLLNLNAD-DRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRVRRRRG-EK 127
V L+ D + +LGEGEV++++ IQE + G+W V+ + G +
Sbjct 71 QSRVIGLDRQPKADLKLLQQILGEGEVAIQV--GGQRPVRIQETVLAGVWWVQLQVGLGE 128
Query 128 LLEDKLEAGCAP-LALWQAATQNLLPTDSLLPPPIDGLMNGLPLAHELLA----HVRNPD 182
++ LE P L +A + P P D L+N P+ ELL H +
Sbjct 129 VVGQWLEVADIPALVRRRAFAEARWPRLGATLP--DDLLNAGPVLVELLEAAKQHAEHAS 186
Query 183 AQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDTLK 242
A PH INL+ LP S D+ FL+ G G + + + GYG I +T +W ++ ++
Sbjct 187 ATPHVINLSLLPFSAEDQRFLAEQLGEGPVTVLSRGYGNCRIASTATPGIWRVQYFNSTD 246
Query 243 GPLLESYEICPIPEVVLAAPEDLVDSAQRLSEV 275
+L++ E+ IP+ AA ED+ DSA+RL E+
Sbjct 247 RLILDTLEVTAIPQAACAAQEDIDDSAERLREI 279
Score = 20.0 bits (40), Expect = 0.37
Identities = 33/142 (23%), Positives = 52/142 (37%), Gaps = 32/142 (23%)
Query 147 TQNLLP-------------TDSLLPP-----------PIDGLMNGLPLAHELLAHVRNP- 181
TQ++LP D L P P ++ P+A LL ++
Sbjct 4 TQSILPPGFGPGSHGEEDRLDYLPMPREMHTFERPSLPEPEQLDSHPIALALLERLQEAL 63
Query 182 -----DAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLR 236
Q I L + P +AD L ++ G G + I+ G I T L VW ++
Sbjct 64 TAYRIGEQSRVIGLDRQP--KADLKLLQQILGEGEVAIQVGGQRPVRIQETVLAGVWWVQ 121
Query 237 CTDTLKGPLLESYEICPIPEVV 258
L + + E+ IP +V
Sbjct 122 LQVGLGEVVGQWLEVADIPALV 143
>P40594 Hydrogenase expression/formation protein HoxQ [Azotobacter
vinelandii]
Length=283
Score = 108 bits (269), Expect = 2e-31
Identities = 82/273 (30%), Positives = 135/273 (49%), Gaps = 14/273 (5%)
Query 10 GPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQLSQRQPPLGE 69
GP + ++ +P + + P++ Q + P +ALL LQ L + +GE
Sbjct 13 GPGSHGEEERPDCPSMPRAMRRFERPALPEPGQLSGHPIALALLERLQEALGAYR--IGE 70
Query 70 VLAVDLLNLNAD-DRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRVRRRRGE-K 127
V L+ D + +LGEGEV++++ + IQE + G+W V+ + G +
Sbjct 71 QSRVIGLDRQPKADLKLLQQILGEGEVAIQV--GGQRPARIQETVLAGVWWVQLQIGRGE 128
Query 128 LLEDKLEAGCAP-LALWQAATQNLLPTDSLLPPPIDGLMNGLPLAHELL----AHVRNPD 182
++ LE P L +A + P LP DGL+N P+ ELL H
Sbjct 129 VVGQWLEVADVPALVRRRAFAETRWPQLGELP---DGLLNAGPVLVELLDAAKRHAERAL 185
Query 183 AQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDTLK 242
A PH++NL+ LP S DR FL+ G G++ + + GYG I +T +W ++ ++
Sbjct 186 ATPHAVNLSLLPFSPEDRRFLAERLGEGSVTLLSRGYGNCRIASTATPGIWCVQYFNSSD 245
Query 243 GPLLESYEICPIPEVVLAAPEDLVDSAQRLSEV 275
+L++ E+ IP+V AA ED+ DSA+RL E+
Sbjct 246 RLILDTLEVTGIPQVACAAQEDIDDSAERLREI 278
Lambda K H a alpha
0.321 0.137 0.422 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 421135
Database: 11561f07322244c662a7c3a46e59d6e1.SwissProt.fasta
Posted date: May 20, 2024 7:00 PM
Number of letters in database: 1,978
Number of sequences in database: 7
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
