BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 1b474d35fd319c6d56334c7730b88dda.SwissProt.fasta
7 sequences; 2,357 total letters
Query= ACIAD3600
Length=215
Score E
Sequences producing significant alignments: (Bits) Value
P13512 Metal cation efflux system protein CzcD [Cupriavidus metal... 63.5 1e-15
P94178 Cation efflux system protein CzcD [Alcaligenes sp. (strain... 63.2 1e-15
Q9ZT63 Metal tolerance protein 1 [Arabidopsis thaliana] 42.4 2e-08
O45923 Zinc transporter ttm-1 [Caenorhabditis elegans] 40.0 1e-07
P55237 Uncharacterized protein in glgB 5'region [Bacillus caldoly... 33.5 2e-06
Q688R1 Metal tolerance protein 1 [Oryza sativa subsp. japonica] 35.8 3e-06
P20107 Vacuolar zinc transporter ZRC1 [Saccharomyces cerevisiae (... 34.7 6e-06
>P13512 Metal cation efflux system protein CzcD [Cupriavidus metallidurans
(strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)]
Length=316
Score = 63.5 bits (153), Expect = 1e-15
Identities = 54/213 (25%), Positives = 96/213 (45%), Gaps = 28/213 (13%)
Query 5 GCCHHHEPPKHQTSKFKIALWIALLLNLGMFFI-EVFGGAHIGSTSLWADALDFLGDSIN 63
G H H+ P KIAL + G F I EV GG S +L +DA L D++
Sbjct 2 GAGHSHDHPGGNERSLKIALALT-----GTFLIAEVVGGVMTKSLALISDAAHMLTDTVA 56
Query 64 YIISIFALGMSL----------YWRATVALFKGLTMGLFALIVLGKVLWSYFYGI----- 108
I++ A+ ++ Y+R F+ L AL++ G ++ +
Sbjct 57 LAIALAAIAIAKRPADKKRTFGYYR-----FEILAAAFNALLLFGVAIYILYEAYLRLKS 111
Query 109 PPDAITMG--IIGLMGLIANVISALVLFAFRDGDANMKSVWLCSRNDAIGNLAIMLAALG 166
PP + G ++ ++GLI N+IS +L + + N+K +L +D +G++ ++ A+
Sbjct 112 PPQIESTGMFVVAVLGLIINLISMRMLSSGQSSSLNVKGAYLEVWSDLLGSVGVIAGAII 171
Query 167 VFGTHSIWPDLVVAFIMAFLGLSAAWTITQQSL 199
+ T W D +A ++ L W + + SL
Sbjct 172 IRFTGWAWVDSAIAVLIGLWVLPRTWILLKSSL 204
>P94178 Cation efflux system protein CzcD [Alcaligenes sp. (strain
CT14)]
Length=316
Score = 63.2 bits (152), Expect = 1e-15
Identities = 54/213 (25%), Positives = 96/213 (45%), Gaps = 28/213 (13%)
Query 5 GCCHHHEPPKHQTSKFKIALWIALLLNLGMFFI-EVFGGAHIGSTSLWADALDFLGDSIN 63
G H H+ P KIAL + G F I EV GG S +L +DA L D++
Sbjct 2 GAGHSHDHPGGNERSLKIALALT-----GTFLIAEVVGGVMTKSLALISDAAHMLTDTVA 56
Query 64 YIISIFALGMSL----------YWRATVALFKGLTMGLFALIVLGKVLWSYFYGI----- 108
I++ A+ ++ Y+R F+ L AL++ G ++ +
Sbjct 57 LAIALAAIAIAKRPADKKRTFGYYR-----FEILAAAFNALLLFGVAIYILYEAYLRLKS 111
Query 109 PPDAITMG--IIGLMGLIANVISALVLFAFRDGDANMKSVWLCSRNDAIGNLAIMLAALG 166
PP + G ++ ++GLI N+IS +L + + N+K +L +D +G++ ++ A+
Sbjct 112 PPQIESTGMFVVAVLGLIINLISMRMLSSGQSSSLNVKGAYLEVWSDLLGSVGVIAGAII 171
Query 167 VFGTHSIWPDLVVAFIMAFLGLSAAWTITQQSL 199
+ T W D +A ++ L W + + SL
Sbjct 172 IRFTGWAWVDSAIAVLIGLWVLPRTWFLLKSSL 204
>Q9ZT63 Metal tolerance protein 1 [Arabidopsis thaliana]
Length=398
Score = 42.4 bits (98), Expect = 2e-08
Identities = 37/137 (27%), Positives = 59/137 (43%), Gaps = 23/137 (17%)
Query 15 HQTSKFKIALWIALLLNLGMFFIEVFGGAHIGSTSLWADALDFLGDSINYIISIFALGMS 74
H+ S L IA++L L +EV GG S ++ DA L D + IS+F+L
Sbjct 49 HERSASMRKLCIAVVLCLVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSL--- 105
Query 75 LYWRATVALFKGLTMGLFALIVLG-----KVLWSYFYGIPPDAI-------------TMG 116
W A T G F + +LG +++W + +AI M
Sbjct 106 --WAAGWEATPRQTYGFFRIEILGALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMF 163
Query 117 IIGLMGLIANVISALVL 133
++ GL+ N+I A++L
Sbjct 164 LVAAFGLVVNIIMAVLL 180
Score = 17.7 bits (34), Expect = 1.8
Identities = 8/18 (44%), Positives = 11/18 (61%), Gaps = 0/18 (0%)
Query 95 IVLGKVLWSYFYGIPPDA 112
I +GKVL + I P+A
Sbjct 353 ITVGKVLLACHVNIRPEA 370
>O45923 Zinc transporter ttm-1 [Caenorhabditis elegans]
Length=410
Score = 40.0 bits (92), Expect = 1e-07
Identities = 48/223 (22%), Positives = 85/223 (38%), Gaps = 41/223 (18%)
Query 8 HHHEPPKHQTSKFKIALWIALLLNLGMFFIEVFGGAHIGSTSLWADALDFLGDSINYIIS 67
H H+ + + + LW L+ E GG S ++ DA L D +++IIS
Sbjct 89 HSHDEKYQKGRRAEKVLWAVAALSAVFIAAEFVGGFWAQSLAIMTDAGHMLSDLLSFIIS 148
Query 68 IFALGMSLYWRATVALFKGLTMGLFALIVLGK-----VLWSYFYGI-------------P 109
IFA+ A + K L+ G VLG +LW +
Sbjct 149 IFAIRC-----ARLPASKRLSFGYERAEVLGALTSVIILWVLTTVLVVVAIQRIVNNEHE 203
Query 110 PDAITMGIIGLMGLIANVISALVLFAFRDG------------------DANMKSVWLCSR 151
DA M I +G++ N++ LVL G + N+++ +
Sbjct 204 VDADVMLITAGVGVLFNIVMGLVLHFGTGGHGHTHGGHSSHGHAHDGKNVNVRAALIHVI 263
Query 152 NDAIGNLAIMLAALGVFGTHSIWPDLVVAFIMAFLGLSAAWTI 194
D + ++ +++AAL + T D + F+ + + L T+
Sbjct 264 GDLVQSIGVLIAALIIRFTGWTLADPICTFLFSIIVLFTTVTV 306
>P55237 Uncharacterized protein in glgB 5'region [Bacillus caldolyticus]
Length=57
Score = 33.5 bits (75), Expect = 2e-06
Identities = 16/46 (35%), Positives = 25/46 (54%), Gaps = 0/46 (0%)
Query 9 HHEPPKHQTSKFKIALWIALLLNLGMFFIEVFGGAHIGSTSLWADA 54
HH P ++ K ALW+ LLL + +E+ GG S +L +D+
Sbjct 9 HHLPHVKTQNESKKALWVTLLLTMFFTAVEIIGGLISNSLALLSDS 54
>Q688R1 Metal tolerance protein 1 [Oryza sativa subsp. japonica]
Length=418
Score = 35.8 bits (81), Expect = 3e-06
Identities = 33/128 (26%), Positives = 54/128 (42%), Gaps = 23/128 (18%)
Query 24 LWIALLLNLGMFFIEVFGGAHIGSTSLWADALDFLGDSINYIISIFALGMSLYWRATVAL 83
L IA++L + +EV GG S ++ DA L D + IS+F+L W A
Sbjct 58 LIIAVILCIIFMAVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSL-----WAAGWEA 112
Query 84 FKGLTMGLFALIVLG-----KVLWSYFYGIPPDAIT-------------MGIIGLMGLIA 125
+ G F + +LG +++W + +AI M + GL
Sbjct 113 TPQQSYGFFRIEILGALVSIQLIWLLAGILVYEAIVRLINESGEVQGSLMFAVSAFGLFV 172
Query 126 NVISALVL 133
N+I A++L
Sbjct 173 NIIMAVLL 180
Score = 18.5 bits (36), Expect = 1.0
Identities = 5/8 (63%), Positives = 6/8 (75%), Gaps = 0/8 (0%)
Query 8 HHHEPPKH 15
HHHE +H
Sbjct 210 HHHEDQEH 217
Score = 16.9 bits (32), Expect = 3.1
Identities = 6/10 (60%), Positives = 6/10 (60%), Gaps = 0/10 (0%)
Query 2 QMAGCCHHHE 11
Q G HHHE
Sbjct 215 QEHGHVHHHE 224
>P20107 Vacuolar zinc transporter ZRC1 [Saccharomyces cerevisiae
(strain ATCC 204508 / S288c)]
Length=442
Score = 34.7 bits (78), Expect = 6e-06
Identities = 19/62 (31%), Positives = 36/62 (58%), Gaps = 3/62 (5%)
Query 142 NMKSVWLCSRNDAIGNLAIMLAALGVFGTHSIW---PDLVVAFIMAFLGLSAAWTITQQS 198
NM V+L DA+GN+ ++ AAL ++ T W D +V+ I+ + S+A +++++
Sbjct 231 NMHGVFLHVLGDALGNIGVIAAALFIWKTEYSWRYYSDPIVSLIITIIIFSSALPLSRRA 290
Query 199 LR 200
R
Sbjct 291 SR 292
Score = 28.1 bits (61), Expect = 9e-04
Identities = 34/133 (26%), Positives = 56/133 (42%), Gaps = 33/133 (25%)
Query 28 LLLNLGMFFIEVFGGAHIGSTSLWADALDFLGDSINYIISIFALGMSLYWRATVALFKG- 86
L L+ F +E+ G S +L AD+ L D I+ ++++ W VA +G
Sbjct 13 LTLDTVFFLLEITIGYMSHSLALIADSFHMLNDIISLLVAL--------WAVDVAKNRGP 64
Query 87 ---LTMGLFALIVLGKVLWSYF-----YGIPPDAITMGI-------------IGLMGLIA 125
T G +LG ++ + F + I +A+ I +G+ GLI+
Sbjct 65 DAKYTYGWKRAEILGALINAVFLIALCFSIMIEALQRLIEPQEIQNPRLVLYVGVAGLIS 124
Query 126 NVISALVLFAFRD 138
NV+ LF F D
Sbjct 125 NVVG---LFLFHD 134
Lambda K H a alpha
0.331 0.143 0.464 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 370107
Database: 1b474d35fd319c6d56334c7730b88dda.SwissProt.fasta
Posted date: May 9, 2024 5:21 PM
Number of letters in database: 2,357
Number of sequences in database: 7
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
