BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 2dc6a1e42ec24e3f031aa893e9f9b317.SwissProt.fasta
11 sequences; 5,798 total letters
Query= ACIAD3432
Length=752
Score E
Sequences producing significant alignments: (Bits) Value
Q60297 Type I restriction enzyme MjaIX methylase subunit [Methano... 112 5e-29
Q57168 Type I restriction enzyme HindI methylase subunit [Haemoph... 103 9e-27
Q57596 Type I restriction enzyme MjaVII methylase subunit [Methan... 100 4e-25
P75436 Type I restriction enzyme MpnII methylase subunit [Mycopla... 95.1 1e-23
Q47163 Type I restriction enzyme EcoprrI methylase subunit [Esche... 93.6 3e-23
P10484 Type I restriction enzyme EcoR124I/EcoR124II methylase sub... 91.7 1e-22
Q58617 Type I restriction enzyme MjaVIII methylase subunit [Metha... 90.9 3e-22
P40813 Type I restriction enzyme StySJI methylase subunit [Salmon... 70.1 1e-15
P08957 Type I restriction enzyme EcoKI methylase subunit [Escheri... 70.1 1e-15
P07989 Type I restriction enzyme StySPI methylase subunit [Salmon... 65.5 3e-14
Q89Z59 Type I restriction enzyme BthVORF4518P methylase subunit [... 60.1 1e-12
>Q60297 Type I restriction enzyme MjaIX methylase subunit [Methanocaldococcus
jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1
/ JCM 10045 / NBRC 100440)]
Length=558
Score = 112 bits (279), Expect = 5e-29
Identities = 120/503 (24%), Positives = 216/503 (43%), Gaps = 73/503 (15%)
Query 24 VWSIANIIRGPYRPPQYRRVMLPLIVLGRFDAILAPYADEMKASYEKAVATLQDKTPNVF 83
+W +A+ +R QY+ V+L LI L E++ + L + P
Sbjct 52 LWKVADKLRKKMEVHQYKYVVLGLIFLRALTCRFYERRKEIEEELSNPNSELYTEDPE-- 109
Query 84 LQKQLSQIAD-----------KDRKQNLY--NISGFNLKKLLDDPDQFTANLTKYIDGFS 130
L+K + + D K+ + + + N+ N+ +++D + KY D
Sbjct 110 LRKMILEDEDFYLSEGVLYLPKETRWDYFVENVMSPNIGEIIDTAIEILEE--KYPD--- 164
Query 131 PKAKDIFAKFEFAKEIEKLDDANRLYKVFQEFRNGLGESGLSLAPSSVSNLQMGYLFEEL 190
+ KD+ K ++ D + + K S +S G ++E
Sbjct 165 -RLKDVIPKIYAQSPLDNHDYSYLINKF----------SEISFGKEHRVKDVFGRIYEYF 213
Query 191 VRKFNEQANEEAGDHFTPREVIELMVNLIFEEDQDELVKAGVHRSIYDPTAGTGGM-LSE 249
+ KF E + G +TPR + +L+V+++ VK G SI+DP G+GG +S
Sbjct 214 LGKFTEVEGKLGGKFYTPRSLTKLIVDVLD-------VKGG---SIFDPACGSGGFFVSA 263
Query 250 SEKFLKKYNDKISLDMYGQEYNPESYAICCSDLLIKDEPAENIVYGDTLGVKNAKEKDGY 309
EK ++ D L +YGQ+ +P +Y + +L+I+ G + D
Sbjct 264 LEKLEREGIDINELSIYGQDSDPMAYRLTKMNLIIR-------------GAEGDIRIDDS 310
Query 310 VPRDGHADKDFHYMFSNPPFG-VEWKNQKDFIDEEEKQGFSGRFGAGLPRINDGSLLFAQ 368
D D F Y+ +NPPF EW + I ++ + G +P + + ++
Sbjct 311 YHDDKFMDMTFDYVVANPPFNDSEWDANR--IKPDDPRLRIGNKKVPVPPNGNANYMWIL 368
Query 369 HMISKMKASPENGGEGSRIAVVFNGSPLFTGDAGSGESNIRRWIIENDWLEAIIALPDQM 428
H I + NG G V+ NG+ AG+ E IR+ IIEND + I+A P ++
Sbjct 369 HFIYH---TAPNGKAGF---VMANGAL----SAGNVEGEIRKAIIENDLVYGIVACPPKL 418
Query 429 FYNTGIYTYIWIISNKKSEQRKGKVQLIDGTAHYQKMAKSLGNKRHELSKAHIAELTKFY 488
FYN + +W I +K + KGKV I+ + + K + +++ L++ HI ++ +
Sbjct 419 FYNVSLPVSLWFIRKEKPDYMKGKVLFINA----KNLYKQISRRQNILTEEHIKKIVDKF 474
Query 489 SKFEDQDTSALIQSKTGEAKICS 511
FE + I ++ G AK+ +
Sbjct 475 RMFESGEDEDKI-NELGFAKVAT 496
Score = 18.9 bits (37), Expect = 8.0
Identities = 7/20 (35%), Positives = 13/20 (65%), Gaps = 0/20 (0%)
Query 108 LKKLLDDPDQFTANLTKYID 127
LKKLLD+ ++ + + +D
Sbjct 535 LKKLLDEEEKLRNKVKEILD 554
>Q57168 Type I restriction enzyme HindI methylase subunit [Haemophilus
influenzae (strain ATCC 51907 / DSM 11121 / KW20 /
Rd)]
Length=443
Score = 103 bits (258), Expect = 9e-27
Identities = 76/277 (27%), Positives = 133/277 (48%), Gaps = 42/277 (15%)
Query 183 MGYLFEELVRKFNEQANEEAGDHFTPREVIELMVNLIFEEDQDELVKAGVHRSIYDPTAG 242
+G+++E + +F + + +G +FTP+ ++ L+V ++ +YDP G
Sbjct 96 LGHVYEYFLSRFAQAEGKRSGQYFTPKSIVSLIVEML----------EPYSGRVYDPAMG 145
Query 243 TGGMLSESEKFLKKYNDKIS-LDMYGQEYNPESYAICCSDLLIKDEPAENIVYGDTLGVK 301
+GG ++E+F+ + I+ + +YGQE+NP ++ + ++ I+ I Y G
Sbjct 146 SGGFFVQTERFITAHQGNINNVSIYGQEFNPTTWKLAAMNMAIR-----GIDYD--FGKY 198
Query 302 NAKEKDGYVPRDGHADKDFHYMFSNPPFG-VEWKNQKDFIDEEEKQGFSGRFGAGLPRIN 360
NA D + + H DK ++ +NP F EW N+ D R+ G P
Sbjct 199 NA---DSFT-QPQHIDKKMDFIMANPHFNDKEWWNESLADDP--------RWAYGTPPKG 246
Query 361 DGSLLFAQHMISKMKASPENGGEGSRIAVVFNGSPLFTGDAGSGESNIRRWIIENDWLEA 420
+ + + QHMI + + G ++ NGS + E IR+ II D +E
Sbjct 247 NANFAWLQHMIYHLSPN------GKIALLLANGS---MSSQTNNEGEIRKAIINADLVEC 297
Query 421 IIALPDQMFYNTGIYTYIWIISNKKSEQRKGKVQLID 457
++ALP Q+F NT I IW ++ K +RKG+V ID
Sbjct 298 MVALPGQLFTNTKIPACIWFLNRNK--KRKGEVLFID 332
Score = 21.6 bits (44), Expect = 1.1
Identities = 11/22 (50%), Positives = 15/22 (68%), Gaps = 0/22 (0%)
Query 728 LEEIKAEIVQLEQEIMQMLGGL 749
L+E A+ +LE EI + LGGL
Sbjct 419 LKEQFAKSAELEAEIKKNLGGL 440
>Q57596 Type I restriction enzyme MjaVII methylase subunit [Methanocaldococcus
jannaschii (strain ATCC 43067 / DSM 2661 /
JAL-1 / JCM 10045 / NBRC 100440)]
Length=577
Score = 100 bits (248), Expect = 4e-25
Identities = 99/380 (26%), Positives = 162/380 (43%), Gaps = 71/380 (19%)
Query 141 EFAKEIEKLDDANRLYKVFQEFRNGLGESGLSLAPSSVSNLQMGYLFEELVRKFNEQANE 200
E A + K+ + N K + LG G N ++ EELVR +NE
Sbjct 172 ELANALNKIANLNDNLKDLSKLVEVLGLIGFI-------NEDNMHILEELVRVYNEMDFS 224
Query 201 EA-----------------------GDHFTPREVIELMVNLIFEEDQDELVKAGVHRSIY 237
E G+ +TP EV+ L+VNL+ E E++
Sbjct 225 EIDYDAIGDAYQWILSYFAPQKCKEGEVYTPVEVVRLIVNLLDIEKDSEVL--------- 275
Query 238 DPTAGTGGMLSESEKFLK-KYNDKISLDMYGQEYNPESYAICCSDLLIKDEPAEN--IVY 294
DP G+G ML ES +++K Y +I L YGQE N + +L++ +E I
Sbjct 276 DPACGSGTMLIESYRYVKDNYGGEIYL--YGQERNEIMAILAKLNLILHGVDSEEYEIYI 333
Query 295 GDTLGVKNAKEKDGYVPRDGHADKDFHYMFSNPPFGVEWKNQKDFIDEEEKQGFSGRF-G 353
GD+L KN K G V Y +NPP+ ++ N+ + + + F
Sbjct 334 GDSL--KNPKIWRGEVD----------YTIANPPWNLDGYNEDVLKENPDVRRIYNTFVR 381
Query 354 AGLPRINDGSLLFAQHMISKMKASPENGGEGSRIAVVFNGSPLFTGDAGSGESNIRRWII 413
G P + Q M+ + ++ +V + LF G E IR+ I+
Sbjct 382 GGYPPKQSADWAWVQLMLYFAR---------KKVGIVLDSGALFRGGK---EKKIRKEIV 429
Query 414 ENDWLEAIIALPDQMFYNTGIYTYIWIISNKKSEQRKGKVQLIDGTAHYQKMAKSLGNKR 473
E D +EAII LP+++FYN + I++ K E+RKGK+ I+ + ++K + +
Sbjct 430 EKDLIEAIILLPEKLFYNVTAPGIVMILNKNKPEERKGKILFINASLEFEKHPEV--RRL 487
Query 474 HELSKAHIAELTKFYSKFED 493
+ L + +I ++ Y +ED
Sbjct 488 NRLGEENIDKIVDVYENWED 507
Score = 20.8 bits (42), Expect = 2.0
Identities = 10/32 (31%), Positives = 16/32 (50%), Gaps = 0/32 (0%)
Query 101 YNISGFNLKKLLDDPDQFTANLTKYIDGFSPK 132
+N+ G+N L ++PD T G+ PK
Sbjct 356 WNLDGYNEDVLKENPDVRRIYNTFVRGGYPPK 387
Score = 19.2 bits (38), Expect = 6.1
Identities = 12/47 (26%), Positives = 23/47 (49%), Gaps = 1/47 (2%)
Query 704 YSKTKVGYEIPINRHFYIYEPPRPLEEIKAEIVQLEQEIMQMLGGLS 750
YSKT+ Y + + +Y+ + EIV+ + IM++ L+
Sbjct 133 YSKTQA-YLMANRSYLTLYDEDEGKLYVWHEIVKSRETIMELANALN 178
>P75436 Type I restriction enzyme MpnII methylase subunit [Mycoplasma
pneumoniae (strain ATCC 29342 / M129 / Subtype 1)]
Length=543
Score = 95.1 bits (235), Expect = 1e-23
Identities = 86/352 (24%), Positives = 152/352 (43%), Gaps = 49/352 (14%)
Query 184 GYLFEELVRKFNEQANEEAGDHFTPREVIELMVNLIFEEDQDELVKAGVHRSIYDPTAGT 243
G +E L+ + + A + G+ FTP+++ EL+ + + K +YD G+
Sbjct 188 GDAYEFLISMYAQNAGKSGGEFFTPQDISELLARIA-------IGKKDTVDDVYDMACGS 240
Query 244 GGMLSESEKFLKKYNDKISL-DMYGQEYNPESYAICCSDLLIK--DEPAENIVYGDTLGV 300
G +L + K L K +K SL YGQE N +Y +C ++++ D NI+ DTL
Sbjct 241 GSLLLQVIKVLGK--EKTSLVSYYGQEINHTTYNLCRMNMILHNIDYANFNIINADTLTT 298
Query 301 KNAKEKDGYVPRDGHADKDFHYMFSNPPFGVEWKNQKDFIDEEEKQGFSGRF--GAGLPR 358
K ++ YV + F + SNPP+ + W D++ RF L
Sbjct 299 KEWEKH--YVNCSN--ENGFEVVVSNPPYSISWAG-----DKKSNLVSDVRFKDAGTLAP 349
Query 359 INDGSLLFAQHMISKMKASPENGGEGSRIAVVFNGSPLFTGDAGSGESNIRRWIIENDWL 418
+ L F H + + G EG+ V F G G E IR+++++ +++
Sbjct 350 NSKADLAFVLHALYVL------GQEGTAAIVCFPGILYREGK----EQTIRKYLVDQNFV 399
Query 419 EAIIALPDQMFYNTGIYTYIWIISNKKSEQRKGKVQLIDGTAHY--------------QK 464
+A+I LP +F T I T I ++ KK+ +K + IDG+ + +K
Sbjct 400 DAVIQLPSNLFSTTSIATSILVL--KKNRDKKDPIFFIDGSNEFVREKKNNRLSPKNIEK 457
Query 465 MAKSLGNKRHELSKAHIAELTKFYSKFEDQDTSALIQSKTGEAKICSKIFNN 516
+ +K+ E + A E K D + S+ + ++ K+ N+
Sbjct 458 IVDCFNSKKEEANFAKSVERDKIRESNYDLTVGKYVNSEAEKEELDIKVLNH 509
Score = 20.0 bits (40), Expect = 3.5
Identities = 27/121 (22%), Positives = 47/121 (39%), Gaps = 24/121 (20%)
Query 84 LQKQLSQIADKDRKQNLYNISGFNLKK--LLDDPDQFTA-NLTKYIDGFSPKAKDIFAKF 140
++K++ +IADK R I G++ K L+ +F + NL KY + + F+
Sbjct 12 VEKKIWEIADKLRG----TIDGWDFKSYVLIGLFYRFLSENLCKYFNDSERRNNPDFSYE 67
Query 141 EFAKEIEKLDDANRLYKVFQEFRNGLGESGLSLAPSSVSNLQMGYLFEELVRKFNEQANE 200
+ E +D + G + PS LF+ +V+ E N
Sbjct 68 NLTDDYEAIDALKDA---------AIASKGFFIKPSQ--------LFQNVVKSIRENKNN 110
Query 201 E 201
E
Sbjct 111 E 111
>Q47163 Type I restriction enzyme EcoprrI methylase subunit [Escherichia
coli]
Length=520
Score = 93.6 bits (231), Expect = 3e-23
Identities = 114/501 (23%), Positives = 215/501 (43%), Gaps = 83/501 (17%)
Query 16 QHGKIVGLVWSIANIIRGPYRPPQYRRVMLPLIVLGRFDAILAPYADEMKASYEKAV--A 73
Q ++ +W IAN +RG +++ +L A+ + E +SY +A +
Sbjct 8 QRAELHRQIWQIANDVRGSVDGWDFKQYVL--------GALFYRFISENFSSYIEAGDDS 59
Query 74 TLQDKTPNVFLQKQLSQIADKDRKQNLYNISGF-NLKKLLDDPDQFTANLTKYIDGFSPK 132
K + + + A K + +Y F N+ + D+ A+L
Sbjct 60 ICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDRLNADLNSIFVAIESS 119
Query 133 A---------KDIFAKFEFAKEIEKLDDANRLYKVFQEFRNGL-----GESGLSLAPSSV 178
A K +FA F+ +NRL ++ L G GL L +
Sbjct 120 AYGYPSEADIKGLFADFDTT--------SNRLGNTVKDKNARLAAVLKGVEGLKLGDFNE 171
Query 179 SNLQM-GYLFEELVRKFNEQANEEAGDHFTPREVIELMVNLIFEEDQDELVKAGVHRSIY 237
+ + G +E L+ + A + G+ FTP+ V +L+ L Q + K IY
Sbjct 172 HQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMH-GQTHVNK------IY 224
Query 238 DPTAGTGGMLSESEKFLKKYNDKISLD-MYGQEYNPESYAICCSDLLIKDEPAE--NIVY 294
DP AG+G +L +++K +++D I + +GQE N +Y + ++ + + + +I
Sbjct 225 DPAAGSGSLLLQAKK---QFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFDIKL 281
Query 295 GDTLGVKNAKEKDGYVPRDGHADKDFHYMFSNPPFGVEWKNQKD--FIDEEEKQGFSGRF 352
G+TL + ++ +K F + SNPP+ V+W D I++E
Sbjct 282 GNTLTEPHFRD-----------EKPFDAIVSNPPYSVKWIGSDDPTLINDER----FAPA 326
Query 353 GAGLPRINDGSLLFAQHMISKMKASPENGGEGSRIAVVFNGSPLFTGDAGSGESNIRRWI 412
G P+ + F H ++ + A R A+V + G A E IR+++
Sbjct 327 GVLAPK-SKADFAFVLHALNYLSAK-------GRAAIVCFPGIFYRGGA---EQKIRQYL 375
Query 413 IENDWLEAIIALPDQMFYNTGIYTYIWIISNKKSEQRKGKVQLIDGTAHYQKMAKSLGNK 472
++N+++E +I+L +F+ T I I ++S K++ KVQ ID + ++K +
Sbjct 376 VDNNYVETVISLAPNLFFGTTIAVNILVLSKHKTDT---KVQFIDASELFKKETNN---- 428
Query 473 RHELSKAHIAELTKFYSKFED 493
+ L+ AHI ++ + ++ ED
Sbjct 429 -NILTDAHIEQIMQVFASKED 448
>P10484 Type I restriction enzyme EcoR124I/EcoR124II methylase
subunit [Escherichia coli]
Length=520
Score = 91.7 bits (226), Expect = 1e-22
Identities = 113/500 (23%), Positives = 210/500 (42%), Gaps = 81/500 (16%)
Query 16 QHGKIVGLVWSIANIIRGPYRPPQYRRVMLPLIVLGRFDAILAPYADEMKASYEKAV--A 73
Q ++ +W IAN +RG +++ +L A+ + E +SY +A +
Sbjct 8 QRAELHRQIWQIANDVRGSVDGWDFKQYVL--------GALFYRFISENFSSYIEAGDDS 59
Query 74 TLQDKTPNVFLQKQLSQIADKDRKQNLYNISGF-NLKKLLDDPDQFTANLTKYIDGFSPK 132
K + + + A K + +Y F N+ + D+ A+L
Sbjct 60 ICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDRLNADLNSIFVAIESS 119
Query 133 A---------KDIFAKFEFAKEIEKLDDANRLYKVFQEFRNGL-----GESGLSLAPSSV 178
A K +FA F+ +NRL ++ L G GL L +
Sbjct 120 AYGYPSEADIKGLFADFDTT--------SNRLGNTVKDKNARLAAVLKGVEGLKLGDFNE 171
Query 179 SNLQM-GYLFEELVRKFNEQANEEAGDHFTPREVIELMVNLIFEEDQDELVKAGVHRSIY 237
+ + G +E L+ + A + G+ FTP+ V +L+ L Q + K IY
Sbjct 172 HQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMH-GQTHVNK------IY 224
Query 238 DPTAGTGGMLSESEKFLKKYNDKISLDMYGQEYNPESYAICCSDLLIKDEPAE--NIVYG 295
DP AG+G +L +++K N I +GQE N +Y + ++ + + + +I G
Sbjct 225 DPAAGSGSLLLQAKKQFD--NHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFDIKLG 282
Query 296 DTLGVKNAKEKDGYVPRDGHADKDFHYMFSNPPFGVEWKNQKD--FIDEEEKQGFSGRFG 353
+TL + ++ +K F + SNPP+ V+W D I++E G
Sbjct 283 NTLTEPHFRD-----------EKPFDAIVSNPPYSVKWIGSDDPTLINDER----FAPAG 327
Query 354 AGLPRINDGSLLFAQHMISKMKASPENGGEGSRIAVVFNGSPLFTGDAGSGESNIRRWII 413
P+ + F H ++ + A R A+V + G A E IR++++
Sbjct 328 VLAPK-SKADFAFVLHALNYLSAK-------GRAAIVCFPGIFYRGGA---EQKIRQYLV 376
Query 414 ENDWLEAIIALPDQMFYNTGIYTYIWIISNKKSEQRKGKVQLIDGTAHYQKMAKSLGNKR 473
+N+++E +I+L +F+ T I I ++S K++ VQ ID + ++K +
Sbjct 377 DNNYVETVISLAPNLFFGTTIAVNILVLSKHKTDT---NVQFIDASELFKKETNN----- 428
Query 474 HELSKAHIAELTKFYSKFED 493
+ L+ AHI ++ + ++ ED
Sbjct 429 NILTDAHIEQIMQVFASKED 448
>Q58617 Type I restriction enzyme MjaVIII methylase subunit [Methanocaldococcus
jannaschii (strain ATCC 43067 / DSM 2661 /
JAL-1 / JCM 10045 / NBRC 100440)]
Length=578
Score = 90.9 bits (224), Expect = 3e-22
Identities = 110/491 (22%), Positives = 212/491 (43%), Gaps = 56/491 (11%)
Query 7 MTNEVNQHSQHGKIVGLVWSIANIIRGPYRPPQYRRVMLPLIVLGRFDAILAPYADEMKA 66
+T E + K++GL+ A++IR Y+ ++L L D L +E+K
Sbjct 74 LTTEKIEKVSKDKLIGLLKQGADLIRTQV---DYKVLLLFLFFKAISDKYLLK-VEELKK 129
Query 67 SYEKAVATLQDKTPNVFLQKQLSQIADKDRKQNLYNISGFNLKKLLDDPDQFTANLTKYI 126
+E L ++ V +++ ++ D + K+ + ++ ++P+ F L K +
Sbjct 130 EFED----LDEEDIYVLANEEILELYDVEGKKL------YVWHEVANNPEDFINALNKIV 179
Query 127 DGFSPKAKDIFAKFEFAKEIEKLDDANRLYKVFQEFRNGLGESGLSLAPSSVSNLQMGYL 186
+ K + + ++ NR + Q N + S A + +G
Sbjct 180 EMNKEKLSGLDELIKRTGLPTLFENENR--HIVQHLINLFSRADFSEASYDI----LGDA 233
Query 187 FEELVRKFNEQANEEAGDHFTPREVIELMVNLIFEEDQDELVKAGVHRSIYDPTAGTGGM 246
+E + F +E G+ +TP EV +L+ +L+ +D DE+ I DP G+G M
Sbjct 234 YEWTLNYFAPTKAKE-GEVYTPIEVSKLIAHLVEPKD-DEV--------ILDPACGSGSM 283
Query 247 LSESEKFLKKYNDKISLDMYGQEYNPESYAICCSDLLIKDEPAEN--IVYGDTLGVKNAK 304
L E +F ++ + GQE N + + + ++ ++ + GD+L +
Sbjct 284 LIEQYRFA---GSNPNIVLVGQERNDVTAVLAKLNFILHGINLKDAKVFIGDSLLNPKFE 340
Query 305 EKDGYVPRDGHADKDFHYMFSNPPFGVEWKNQKDFIDEEEKQGFSGRFGAGLPRINDGSL 364
V G ADK + +NPP+ + ++ E+ + + G P N
Sbjct 341 SFIXEVKGTGKADK----VVANPPWNQDGYDENTLKVNEK---YKDIYMYGFPNKNSADW 393
Query 365 LFAQHMISKMKASPENGGEGSRIAVVFNGSPLFTGDAGSGESNIRRWIIENDWLEAIIAL 424
+ Q + N + +V + LF G E IR+ +++D +EA++ L
Sbjct 394 AWVQLI---------NYYTEKKAGIVLDSGALFRGGK---EKTIRKRFVDDDLIEAVVLL 441
Query 425 PDQMFYNTGIYTYIWIISNKKSEQRKGKVQLIDGTAHYQKMAKSLGNKRHELSKAHIAEL 484
P+++FYN I I++ K E+RKGK+ I+ + Y K + K ++LS +I ++
Sbjct 442 PEKLFYNCPAPGIILILNKNKPEERKGKILFINASNEYIKHPEV--KKLNKLSDENIEKI 499
Query 485 TKFYSKFEDQD 495
K Y +F+D D
Sbjct 500 AKAYKEFKDVD 510
Score = 23.1 bits (48), Expect = 0.40
Identities = 20/81 (25%), Positives = 38/81 (47%), Gaps = 4/81 (5%)
Query 669 DKEPDLSKLLPLVKAHCEAYLKAEVLPHVADAWIDYSKTKVGYEIPINRHFYIYEPPRPL 728
DK + K+ PL E K +++ + D +T+V Y++ + F+ + L
Sbjct 63 DKRKKIYKIKPLTTEKIEKVSKDKLIGLLKQG-ADLIRTQVDYKVLLLFLFFKAISDKYL 121
Query 729 ---EEIKAEIVQLEQEIMQML 746
EE+K E L++E + +L
Sbjct 122 LKVEELKKEFEDLDEEDIYVL 142
>P40813 Type I restriction enzyme StySJI methylase subunit [Salmonella
typhimurium (strain LT2 / SGSC1412 / ATCC 700720)]
Length=529
Score = 70.1 bits (170), Expect = 1e-15
Identities = 70/273 (26%), Positives = 116/273 (42%), Gaps = 64/273 (23%)
Query 184 GYLFEELVRKFNEQANEEAGDHFTPREVIELMVNLIFEEDQDELVKAGVHRSIYDPTAGT 243
G ++E L++K + AG +FTPR +I+ +++L+ K + DP AGT
Sbjct 128 GDMYEGLLQKNANETKSGAGQYFTPRPLIKTIIHLL---------KPQPREVVQDPAAGT 178
Query 244 GGMLSESEKFLK-KYNDKISLD-----------MYGQEYNPESYAICCSDLLIKDEPAEN 291
G L E+++++K + ND LD G E P + + + L+ D N
Sbjct 179 AGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELVPGTRRLALMNCLLHDIEG-N 237
Query 292 IVYGDTLGVKNAKEKDG-YVPRDGHADKDFHYMFSNPPFGVEWKNQKDFIDEEEKQGFSG 350
+ +G + + N DG +P+ H + +NPPFG
Sbjct 238 LDHGGAIRLGNTLGSDGENLPK-------AHIVATNPPFG-------------------S 271
Query 351 RFGAGLPR-----INDGSLLFAQHMISKMKASPENGGEGSRIAVVFNGSPLFTGDAGSGE 405
G + R ++ L F QH+I + G R AVV + LF G G
Sbjct 272 AAGTNITRTFVHPTSNKQLCFMQHIIETLH-------PGGRAAVVVPDNVLFEGGKG--- 321
Query 406 SNIRRWIIENDWLEAIIALPDQMFYNTGIYTYI 438
++IRR +++ L I+ LP +FY G+ T +
Sbjct 322 TDIRRDLMDKCHLHTILRLPTGIFYAQGVKTNV 354
Score = 21.6 bits (44), Expect = 1.2
Identities = 11/39 (28%), Positives = 21/39 (54%), Gaps = 5/39 (13%)
Query 499 LIQSKTGEAKICSKIFNNQDFGYLKLTVERPLRLNFTIS 537
L+Q + E K+ +F+N + T+E+P +L +S
Sbjct 75 LVQLGSDEKKLVQAVFHN-----VSTTIEQPKQLTELVS 108
Score = 19.6 bits (39), Expect = 4.6
Identities = 9/47 (19%), Positives = 18/47 (38%), Gaps = 0/47 (0%)
Query 464 KMAKSLGNKRHELSKAHIAELTKFYSKFEDQDTSALIQSKTGEAKIC 510
++ +LG+ L KAHI + + + T ++C
Sbjct 245 RLGNTLGSDGENLPKAHIVATNPPFGSAAGTNITRTFVHPTSNKQLC 291
>P08957 Type I restriction enzyme EcoKI methylase subunit [Escherichia
coli (strain K12)]
Length=529
Score = 70.1 bits (170), Expect = 1e-15
Identities = 70/273 (26%), Positives = 116/273 (42%), Gaps = 64/273 (23%)
Query 184 GYLFEELVRKFNEQANEEAGDHFTPREVIELMVNLIFEEDQDELVKAGVHRSIYDPTAGT 243
G ++E L++K + AG +FTPR +I+ +++L+ K + DP AGT
Sbjct 128 GDMYEGLLQKNANETKSGAGQYFTPRPLIKTIIHLL---------KPQPREVVQDPAAGT 178
Query 244 GGMLSESEKFLK-KYNDKISLD-----------MYGQEYNPESYAICCSDLLIKDEPAEN 291
G L E+++++K + ND LD G E P + + + L+ D N
Sbjct 179 AGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELVPGTRRLALMNCLLHDIEG-N 237
Query 292 IVYGDTLGVKNAKEKDG-YVPRDGHADKDFHYMFSNPPFGVEWKNQKDFIDEEEKQGFSG 350
+ +G + + N DG +P+ H + +NPPFG
Sbjct 238 LDHGGAIRLGNTLGSDGENLPK-------AHIVATNPPFG-------------------S 271
Query 351 RFGAGLPR-----INDGSLLFAQHMISKMKASPENGGEGSRIAVVFNGSPLFTGDAGSGE 405
G + R ++ L F QH+I + G R AVV + LF G G
Sbjct 272 AAGTNITRTFVHPTSNKQLCFMQHIIETLH-------PGGRAAVVVPDNVLFEGGKG--- 321
Query 406 SNIRRWIIENDWLEAIIALPDQMFYNTGIYTYI 438
++IRR +++ L I+ LP +FY G+ T +
Sbjct 322 TDIRRDLMDKCHLHTILRLPTGIFYAQGVKTNV 354
Score = 19.6 bits (39), Expect = 4.6
Identities = 9/47 (19%), Positives = 18/47 (38%), Gaps = 0/47 (0%)
Query 464 KMAKSLGNKRHELSKAHIAELTKFYSKFEDQDTSALIQSKTGEAKIC 510
++ +LG+ L KAHI + + + T ++C
Sbjct 245 RLGNTLGSDGENLPKAHIVATNPPFGSAAGTNITRTFVHPTSNKQLC 291
>P07989 Type I restriction enzyme StySPI methylase subunit [Salmonella
potsdam]
Length=529
Score = 65.5 bits (158), Expect = 3e-14
Identities = 70/273 (26%), Positives = 116/273 (42%), Gaps = 64/273 (23%)
Query 184 GYLFEELVRKFNEQANEEAGDHFTPREVIELMVNLIFEEDQDELVKAGVHRSIYDPTAGT 243
G ++E L++K + AG +FTPR +I+ +++L+ K + DP AGT
Sbjct 128 GDMYEGLLQKNANETKSGAGQYFTPRPLIKTIIHLL---------KPQPREVVQDPAAGT 178
Query 244 GGMLSESEKFLK-KYNDKISLD-----------MYGQEYNPESYAICCSDLLIKDEPAEN 291
G L E+++++K + ND LD G E P + + + L+ D N
Sbjct 179 AGFLIEADRYVKSQTNDLDDLDGDAQDFQIKKAFVGLELVPGTRRLALMNCLLHDIEG-N 237
Query 292 IVYGDTLGVKNAKEKDG-YVPRDGHADKDFHYMFSNPPFGVEWKNQKDFIDEEEKQGFSG 350
+ +G + + N DG +P+ AD + +NPPFG
Sbjct 238 LDHGGAIRLGNTLGSDGENLPQ---AD----IVATNPPFG-------------------S 271
Query 351 RFGAGLPR-----INDGSLLFAQHMISKMKASPENGGEGSRIAVVFNGSPLFTGDAGSGE 405
G + R ++ L F QH+I + G R A V + LF G G
Sbjct 272 AAGTNITRTFVHPTSNKQLCFMQHIIETLP-------PGGRAAAVVPDNVLFEGGKG--- 321
Query 406 SNIRRWIIENDWLEAIIALPDQMFYNTGIYTYI 438
++IRR +++ L I+ LP +FY G+ T +
Sbjct 322 TDIRRDLMDKCHLHTILRLPTGIFYAQGVKTNV 354
>Q89Z59 Type I restriction enzyme BthVORF4518P methylase subunit
[Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079
/ JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)]
Length=472
Score = 60.1 bits (144), Expect = 1e-12
Identities = 69/273 (25%), Positives = 114/273 (42%), Gaps = 63/273 (23%)
Query 184 GYLFEELVRKFNEQANEEAGDHFTPREVIELMVNLIFEEDQDELVKAGVHRSIYDPTAGT 243
G ++E ++ K + AG +FTPR +I+ MV+ I + + ++ DP GT
Sbjct 131 GAIYESILEKNGQDKKSGAGQYFTPRPLIQAMVDCINPQ---------MGETVCDPACGT 181
Query 244 GGMLSESEKFLKKYN-DKISLD------MYGQEYNPESYAICCSDLLIKDEPAEN--IVY 294
GG L + ++K + K D ++G + P + +L + + IV
Sbjct 182 GGFLLTAYDYMKGQSASKEKRDFLRDKALHGVDNTPLVVTLASMNLYLHGIGTDRSPIVC 241
Query 295 GDTLGVKNAKEKDGYVPRDGHADKDFHYMFSNPPFGVEWK-----NQKDFIDEEEKQGFS 349
D+L KE V + +NPPFG N+ DF E +
Sbjct 242 EDSL----EKEPSTLV----------DVILANPPFGTRPAGSVDINRPDFYVETK----- 282
Query 350 GRFGAGLPRINDGSLLFAQHMISKMKASPENGGEGSRIAVVFNGSPLFTGDAGSGESNIR 409
+ L F QHM+ +K G R AVV + LF +AG+GE+ IR
Sbjct 283 -----------NNQLNFLQHMMLMLKT-------GGRAAVVLPDNVLF--EAGAGET-IR 321
Query 410 RWIIENDWLEAIIALPDQMFYNTGIYTYIWIIS 442
+ ++++ L I+ LP +FY G+ + S
Sbjct 322 KRLLQDFNLHTILRLPTGIFYAQGVKANVLFFS 354
Lambda K H a alpha
0.317 0.136 0.393 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 3632392
Database: 2dc6a1e42ec24e3f031aa893e9f9b317.SwissProt.fasta
Posted date: May 9, 2024 11:04 AM
Number of letters in database: 5,798
Number of sequences in database: 11
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
