BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: ac2e046f8e6e03ee3af449964bd01c3d.SwissProt.fasta
8 sequences; 3,589 total letters
Query= ACIAD3431
Length=449
Score E
Sequences producing significant alignments: (Bits) Value
P07990 Type I restriction enzyme StySPI specificity subunit [Salm... 92.8 8e-24
Q57594 Type I restriction enzyme MjaVII specificity subunit [Meth... 92.0 1e-23
Q58615 Type I restriction enzyme MjaVIII specificity subunit [Met... 79.3 2e-19
P06990 Type I restriction enzyme EcoBI specificity subunit [Esche... 78.2 5e-19
P05719 Type I restriction enzyme EcoKI specificity subunit [Esche... 77.4 9e-19
Q58926 Putative type I restriction enzyme MjaX specificity subuni... 77.0 1e-18
P06187 Type I restriction enzyme StySJI specificity subunit [Salm... 60.5 2e-13
P06991 Type I restriction enzyme EcoDI specificity subunit [Esche... 49.3 8e-10
>P07990 Type I restriction enzyme StySPI specificity subunit [Salmonella
potsdam]
Length=463
Score = 92.8 bits (229), Expect = 8e-24
Identities = 102/402 (25%), Positives = 180/402 (45%), Gaps = 34/402 (8%)
Query 53 QPRYIRITDINDSGTLREDTFKSLEIEK---AQEYLLNDLDILLARSGAT---VGKSYLH 106
Q Y+ I N+ + DT + + K + ++ DI++A S + VGKS
Sbjct 37 QDDYLPIIRANNIQNGKFDTTDLVFVPKNLVKESQKISPEDIVIAMSSGSKSVVGKS--- 93
Query 107 KKDKVNVACYAGYLIRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYN 166
++ C G A ++ P +I F +S Y + I S++ A I N+ ++
Sbjct 94 AHQRLPFECSFGAFCGALRPEKFISPNYIAHFTKSSFYRNKISSLSAGANINNIKPASFD 153
Query 167 DLALSIPSLAEQKIIADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDV 226
+ + IPSLAEQKIIA+ LD LAQVD+ A+ E + + L R A+++ AV+ L +
Sbjct 154 LINIPIPSLAEQKIIAEKLDTLLAQVDSTKARLEQIPQILKRFRQAVLAAAVSGTLTTAL 213
Query 227 EMKESDVVLLGNIPNTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGN- 285
S L+G W+ L L+ + A + ++ +R+ D D+
Sbjct 214 RNSHS---LIG-----WHSTNLGALIVDSCNGLAKRQGLNGNEIT--ILRLADFKDAQRI 263
Query 286 LKDETFKSLESEKAQEYLLDDLDILLAR---SGATVGKSYLYKAESVGIACYAGYLIRAR 342
+ +E L+S++ +Y L++ DIL+ R S G+ YK+ I + + IR R
Sbjct 264 IGNERKIKLDSKEENKYSLENDDILVIRVNGSADLAGRFIEYKSNG-DIEGFCDHFIRLR 322
Query 343 LDQENYNPEFVNYFLQSKQYWDWI-SSINIQATIQNVSAEKYNDLTLAIPSLEEQ----- 396
LD F+ Y + ++ +S++ A ++ L+ +P L+EQ
Sbjct 323 LDSNKIMSRFLTYIANEGEGRFYLRNSLSTSAGQNTINQTSIKGLSFLLPPLKEQAEIVR 382
Query 397 --KQLIEYLKNEDEKFNRAISKGKKLVH--LLNEYRSTLITQ 434
+QL Y +++ N A+++ L L +R L Q
Sbjct 383 RVEQLFAYADTIEKQVNNALTRVNSLTQSILAKAFRGELTAQ 424
Score = 50.8 bits (120), Expect = 3e-10
Identities = 34/136 (25%), Positives = 68/136 (50%), Gaps = 6/136 (4%)
Query 308 DILLARSGAT---VGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSKQYWD 364
DI++A S + VGKS + + C G A ++ +P ++ +F +S Y +
Sbjct 77 DIVIAMSSGSKSVVGKS---AHQRLPFECSFGAFCGALRPEKFISPNYIAHFTKSSFYRN 133
Query 365 WISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKLVHLL 424
ISS++ A I N+ ++ + + IPSL EQK + E L + + ++ +++ +L
Sbjct 134 KISSLSAGANINNIKPASFDLINIPIPSLAEQKIIAEKLDTLLAQVDSTKARLEQIPQIL 193
Query 425 NEYRSTLITQVVTGKI 440
+R ++ V+G +
Sbjct 194 KRFRQAVLAAAVSGTL 209
Score = 17.3 bits (33), Expect = 8.4
Identities = 9/24 (38%), Positives = 10/24 (42%), Gaps = 0/24 (0%)
Query 16 QWLGEIPSHWEVKRMKFLLSEKLK 39
QW E P K L EK+K
Sbjct 424 QWRAENPDLISGKNSAAALLEKIK 447
>Q57594 Type I restriction enzyme MjaVII specificity subunit [Methanocaldococcus
jannaschii (strain ATCC 43067 / DSM 2661
/ JAL-1 / JCM 10045 / NBRC 100440)]
Length=425
Score = 92.0 bits (227), Expect = 1e-23
Identities = 102/440 (23%), Positives = 198/440 (45%), Gaps = 42/440 (10%)
Query 18 LGEIPSHWEVKRMKFLLSEKLKYGANESAESED---KDQPRYIRITDINDSGTLREDT-F 73
+GEIP WE+ +K + +K+K G E+ +++I DI +S +T
Sbjct 13 IGEIPEDWEIVELKDVC-KKIKAGGTPKTSVEEYYKNGTIPFVKIEDITNSNKYLTNTKI 71
Query 74 KSLE--IEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYLIRARFNKENYD 131
K E + + +++ +L A G+ +G++ ++K + G + + + +
Sbjct 72 KITEEGLNNSNAWIVPKNSVLFAMYGS-IGETAINKIEVATNQAILGIIPKDNILESEF- 129
Query 132 PQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKIIADFLDKRLAQ 191
+ ++K Y+S + +Q T +N++A+ + +P L EQK IA L K
Sbjct 130 --LYYILAKNKNYYS---KLGMQTTQKNLNAQIVKSFKIPLPPLEEQKQIAKILTK---- 180
Query 192 VDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIPNTWNIKRLKFL 251
+D I E + KL + L+ +TKG+ K+S++ G IP W + +K +
Sbjct 181 IDEGIEIIEKSINKLERIKKGLMHKLLTKGIGHS-RFKKSEI---GEIPEDWEVFEIKDI 236
Query 252 LSEKLKYGANESAE-SEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQEYLLDDLDIL 310
++K G S + SE EN IT +D S L ++ + K + L+ ++
Sbjct 237 F--EVKTGTTPSTKKSEYWENGEINWITPLDLS-RLNEKIYIGSSERKVTKIALEKCNLN 293
Query 311 LARSG-------ATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSKQYW 363
L G A VG + ES G + + ++ N EF Y+L+ K+
Sbjct 294 LIPKGSIIISTRAPVGYVAVLTVESTFNQGCKGLFQK---NNDSVNTEFYAYYLKFKK-- 348
Query 364 DWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKLVHL 423
+ + +++ +T + +S + + +P LEEQKQ+ + L + D+ K +KL +
Sbjct 349 NLLENLSGGSTFKELSKSMLENFKIPLPPLEEQKQIAKILSSVDKSIELKKQKKEKLQRM 408
Query 424 LNEYRSTLITQVVTGKIDVQ 443
+ ++ ++TGK+ V+
Sbjct 409 ----KKKIMELLLTGKVRVK 424
Score = 52.8 bits (125), Expect = 6e-11
Identities = 51/217 (24%), Positives = 99/217 (46%), Gaps = 18/217 (8%)
Query 236 LGNIPNTWNIKRLKFLLSEKLKYGANESAESEDKENPR--YIRITDIDDSGNLKDETFKS 293
+G IP W I LK + + G +++ E +N +++I DI +S T
Sbjct 13 IGEIPEDWEIVELKDVCKKIKAGGTPKTSVEEYYKNGTIPFVKIEDITNSNKYLTNTKIK 72
Query 294 LESE---KAQEYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNP 350
+ E + +++ +L A G+ +G++ + K E G + + + +
Sbjct 73 ITEEGLNNSNAWIVPKNSVLFAMYGS-IGETAINKIEVATNQAILGIIPKDNILES---- 127
Query 351 EFVNYFL-QSKQYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEK 409
EF+ Y L ++K Y+ S + +Q T +N++A+ + +P LEEQKQ+ + L DE
Sbjct 128 EFLYYILAKNKNYY---SKLGMQTTQKNLNAQIVKSFKIPLPPLEEQKQIAKILTKIDE- 183
Query 410 FNRAISKGKKLVHLLNEYRSTLITQVVTGKIDVQNLK 446
I +K ++ L + L+ +++T I K
Sbjct 184 ---GIEIIEKSINKLERIKKGLMHKLLTKGIGHSRFK 217
>Q58615 Type I restriction enzyme MjaVIII specificity subunit
[Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661
/ JAL-1 / JCM 10045 / NBRC 100440)]
Length=425
Score = 79.3 bits (194), Expect = 2e-19
Identities = 98/437 (22%), Positives = 188/437 (43%), Gaps = 46/437 (11%)
Query 21 IPSHWEVKRMKFLLSEKLKY--GANESAESEDKDQPRYIRITDINDSGTLREDTFKSLEI 78
+P WEV R + + +KY G + +++ + Y ++ + LR+
Sbjct 21 VPEDWEVVR----IGDFIKYIKGKKPAVMVDEELEGYYPYLS----TEYLRDGIASKFVK 72
Query 79 EKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYLIRARFNKENYDPQFINLF 138
+E ++N+ DILL G+ G+ +L KK ++ + ++N + LF
Sbjct 73 ITNKEIIVNENDILLLWDGSNAGEIFLGKKGILSSTM-------VKLEQKNKIMDDLYLF 125
Query 139 LQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKIIADFLDKRLAQVDALIAK 198
K S+++S I +V + + ++ + +P L EQK IA K L+ D LI
Sbjct 126 YSLKLKESFLKSQTKGTGIPHVDKKIFENIKIPLPPLEEQKQIA----KILSDFDNLIGT 181
Query 199 QETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIPNTWNIKRLKFLLSEKLKY 258
+E L + + ++ TKG+ K+S++ G IP W + +LK ++ ++
Sbjct 182 INKQIEVLNKAKKGMMKKLFTKGVFEHKSFKKSEI---GEIPEDWEVVKLKEVVD--IQS 236
Query 259 GANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQEY---LLDDLDILLARSG 315
G + + ++I ++ G + ET L + +Y +L DI+LA +
Sbjct 237 GKYFKYSEFCENGVKCLKIDNVG-FGKIFWETVSFLPEDYLNKYPQLVLKSGDIVLALNR 295
Query 316 ATVGKSYLYKAESVGI-------ACYAGYLIRARLDQENYNPEFVNYFLQSKQYWDWISS 368
+G +GI A + R E + +F+ Y L S+ + +S
Sbjct 296 PIIGGKI-----KIGILKDIDEPAILYQRVGRFIFKSEKIDKQFLFYLLMSEYFKKELSK 350
Query 369 INIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKLVHLLNEYR 428
+ I + ++ + +P LEEQK + E LK+ D I +K + + +
Sbjct 351 LLIGTDQPYIRTPVLLNIKIPLPHLEEQKAMAERLKSID----NLIEIKRKEKEQIEKAK 406
Query 429 STLITQVVTGKIDVQNL 445
++ ++TGKI V+NL
Sbjct 407 KKIMNLLLTGKIRVKNL 423
Score = 38.9 bits (89), Expect = 1e-06
Identities = 50/216 (23%), Positives = 96/216 (44%), Gaps = 18/216 (8%)
Query 7 YESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRITDINDSG 66
++S+K S + GEIP WEV ++K ++ ++ G ++ + ++I ++ G
Sbjct 208 HKSFKKSEI---GEIPEDWEVVKLKEVVD--IQSGKYFKYSEFCENGVKCLKIDNVG-FG 261
Query 67 TLREDTFKSLE---IEKAQEYLLNDLDILLARSGATVGKSYL--HKKDKVNVACYAGYLI 121
+ +T L + K + +L DI+LA + +G KD A +
Sbjct 262 KIFWETVSFLPEDYLNKYPQLVLKSGDIVLALNRPIIGGKIKIGILKDIDEPAILYQRVG 321
Query 122 RARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKII 181
R F E D QF+ L S+ + + + I + ++ + +P L EQK +
Sbjct 322 RFIFKSEKIDKQFLFYLLMSEYFKKELSKLLIGTDQPYIRTPVLLNIKIPLPHLEEQKAM 381
Query 182 ADFLDKRLAQVDALI---AKQETLLEKLAEQRVALI 214
A+ RL +D LI K++ +EK ++ + L+
Sbjct 382 AE----RLKSIDNLIEIKRKEKEQIEKAKKKIMNLL 413
>P06990 Type I restriction enzyme EcoBI specificity subunit [Escherichia
coli]
Length=474
Score = 78.2 bits (191), Expect = 5e-19
Identities = 97/402 (24%), Positives = 181/402 (45%), Gaps = 35/402 (9%)
Query 51 KDQPRYIRITDINDSGTLREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDK 110
KD IRI D+ T T+ S +I + Y + D+++ G + +
Sbjct 51 KDGVPLIRIRDVLKGNT---STYYSGQIPEG--YWVYPEDLIVGMDGDFNATIWCSEPAL 105
Query 111 VNVACYAGYLIRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLAL 170
+N + + ++ Y+ +F L Y S I + T++++S+ D L
Sbjct 106 LNQR-----VCKIEVQEDKYNKRFFYHALPG--YLSAINANTSSVTVKHLSSRTLQDTLL 158
Query 171 SIPSLAEQKIIADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKE 230
+P LAEQKIIA+ LD LAQVD+ A+ E + + L R A+++ AVT L + +
Sbjct 159 PLPPLAEQKIIAEKLDTLLAQVDSTKARLEQIPQILKRFRQAVLAAAVTGRLTKEDKDFI 218
Query 231 SDVVLLGN----IPNTWNIKRLKFLLSEK--LKYGANESAESEDKENPRYIRITDIDDSG 284
+ V L N IP W+ L +++ + L YG + + + K+ IR+ DI+D G
Sbjct 219 TKKVELDNYKILIPEDWSETILNNIINTQRPLCYGVVQPGD-DIKDGIELIRVCDIND-G 276
Query 285 NLKDETFKSLESEKAQEYLLDDL---DILLARSGATVGKSYLYKAESVGIACYAGYLIRA 341
+ + + E +Y + DIL+ GA +G+ + + E + + A + R
Sbjct 277 EVDLNHLRKISKEIDLQYKRSKVRKNDILVTIVGA-IGRIGIVR-EDINVN-IARAVARI 333
Query 342 RLDQENYNPEFVNYFLQSKQYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQ----- 396
+ + P F++ +L S W+ + + + ++ + + + +PS+EEQ
Sbjct 334 SPEYKIIVPMFLHIWLSSPVMQTWLVQSSKEVARKTLNLKDLKNAFVPLPSIEEQHEIVR 393
Query 397 --KQLIEYLKNEDEKFNRAISKGKKLVH--LLNEYRSTLITQ 434
+QL Y + +++ N A+++ L L +R L Q
Sbjct 394 RVEQLFAYADSIEKQVNNALARVNNLTQSILAKAFRGELTAQ 435
Score = 40.0 bits (92), Expect = 7e-07
Identities = 46/222 (21%), Positives = 101/222 (45%), Gaps = 16/222 (7%)
Query 21 IPSHWEVKRMKFLLSEK--LKYGANESAESEDKDQPRYIRITDINDSGTLREDTFKSLEI 78
IP W + +++ + L YG + + + KD IR+ DIND G + + + +
Sbjct 231 IPEDWSETILNNIINTQRPLCYGVVQPGD-DIKDGIELIRVCDIND-GEVDLNHLRKISK 288
Query 79 EKAQEYLLNDL---DILLARSGATVGKSYLHKKD-KVNVACYAGYLIRARFNKEN--YDP 132
E +Y + + DIL+ GA +G+ + ++D VN+A AR + E P
Sbjct 289 EIDLQYKRSKVRKNDILVTIVGA-IGRIGIVREDINVNIARAV-----ARISPEYKIIVP 342
Query 133 QFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKIIADFLDKRLAQV 192
F++++L S +W+ + + + ++ + + + +PS+ EQ I +++ A
Sbjct 343 MFLHIWLSSPVMQTWLVQSSKEVARKTLNLKDLKNAFVPLPSIEEQHEIVRRVEQLFAYA 402
Query 193 DALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVV 234
D++ + L ++ ++++ A L + D++
Sbjct 403 DSIEKQVNNALARVNNLTQSILAKAFRGELTAQWRAENPDLI 444
>P05719 Type I restriction enzyme EcoKI specificity subunit [Escherichia
coli (strain K12)]
Length=464
Score = 77.4 bits (189), Expect = 9e-19
Identities = 107/442 (24%), Positives = 190/442 (43%), Gaps = 46/442 (10%)
Query 19 GEIPSHWEVKRMKFL--LSEKLKYGANESAESEDKDQPRYIRITDINDSGTLREDTFKSL 76
G++P W + + + L + Y ++ D IR +I + + DT +
Sbjct 4 GKLPEGWVIAPVSTVTTLIRGVTYKKEQAINYLKDDYLPLIRANNIQNG---KFDTTDLV 60
Query 77 EIEK---AQEYLLNDLDILLARSGAT---VGKS-YLHKKDKVNVACYAGYLIRARFNKEN 129
+ K + ++ DI++A S + VGKS + H + + + G L +
Sbjct 61 FVPKNLVKESQKISPEDIVIAMSSGSKSVVGKSAHQHLPFECSFGAFCGVLRPEKLIFSG 120
Query 130 YDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKIIADFLDKRL 189
FI F +S Y + I S++ A I N+ ++ + + IP LAEQKIIA+ LD L
Sbjct 121 ----FIAHFTKSSLYRNKISSLSAGANINNIKPASFDLINIPIPPLAEQKIIAEKLDTLL 176
Query 190 AQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIPNTWNIKRLK 249
AQVD+ A+ E + + L R A++ AV L E P K+L
Sbjct 177 AQVDSTKARFEQIPQILKRFRQAVLGGAVNGKLTEKWRNFE---------PQHSVFKKLN 227
Query 250 F--LLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQ--EYLLD 305
F +L+E L+ G + +P +RI+ + +G++ + LE +++ + L
Sbjct 228 FESILTE-LRNGLSSKPNESGVGHP-ILRISSV-RAGHVDQNDIRFLECSESELNRHKLQ 284
Query 306 DLDILLARSGAT---VGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSKQY 362
D D+L R + VG L K Y LIRARL ++ PE++ F S
Sbjct 285 DGDLLFTRYNGSLEFVGVCGLLKKLQHQNLLYPDKLIRARLTKDAL-PEYIEIFFSSPSA 343
Query 363 WD-WISSINIQATIQNVSAEKYNDLTLAIPSLEEQ-------KQLIEYLKNEDEKFNRAI 414
+ ++ + + + +S + + +P ++EQ +QL Y +++ N A+
Sbjct 344 RNAMMNCVKTTSGQKGISGKDIKSQVVLLPPVKEQAEIVRRVEQLFAYADTIEKQVNNAL 403
Query 415 SKGKKLVH--LLNEYRSTLITQ 434
++ L L +R L Q
Sbjct 404 ARVNNLTQSILAKAFRGELTAQ 425
Score = 44.7 bits (104), Expect = 2e-08
Identities = 33/136 (24%), Positives = 64/136 (47%), Gaps = 6/136 (4%)
Query 308 DILLARSGAT---VGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSKQYWD 364
DI++A S + VGKS + + C G ++ F+ +F +S Y +
Sbjct 77 DIVIAMSSGSKSVVGKS---AHQHLPFECSFGAFCGVLRPEKLIFSGFIAHFTKSSLYRN 133
Query 365 WISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKLVHLL 424
ISS++ A I N+ ++ + + IP L EQK + E L + + ++ +++ +L
Sbjct 134 KISSLSAGANINNIKPASFDLINIPIPPLAEQKIIAEKLDTLLAQVDSTKARFEQIPQIL 193
Query 425 NEYRSTLITQVVTGKI 440
+R ++ V GK+
Sbjct 194 KRFRQAVLGGAVNGKL 209
>Q58926 Putative type I restriction enzyme MjaX specificity subunit
[Methanocaldococcus jannaschii (strain ATCC 43067 / DSM
2661 / JAL-1 / JCM 10045 / NBRC 100440)]
Length=425
Score = 77.0 bits (188), Expect = 1e-18
Identities = 106/435 (24%), Positives = 193/435 (44%), Gaps = 37/435 (9%)
Query 18 LGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRITDINDS--GTLREDTFKS 75
+GEIP WEV+ +K +L E ++ G + +++DK RI I+DS + +
Sbjct 18 IGEIPEDWEVRELKDIL-EVIRNGLT-AKQNKDKIGYPITRIETISDSKIDITKLGYVED 75
Query 76 LEIEKAQEYLLNDLDILLA--RSGATVGKSYLHKKDKVNVACYAGYLIRARFNKENYDPQ 133
++ E +Y L DIL + S +GK ++ + K + L+ R NK +P
Sbjct 76 IKQEDIAKYRLIIGDILFSHINSEEHIGKVAIY-EGKPEFLLHGMNLLLLRPNKNKIEPY 134
Query 134 FINLFLQSKAYWSWIESVNIQATIQ-NVSAEKYNDLALSIPSLAEQKIIADFLDKRLAQV 192
++ L+ + + + +A Q +++ + L + +P L EQK IA K L+
Sbjct 135 YLLYLLRHFKQKNIFKYIAKRAVNQSSINQTQLKHLKIPLPPLEEQKQIA----KILSDF 190
Query 193 DALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIPNTWNIKRLKFLL 252
D LI +E L + + ++ TKG+ K+S++ G IP W + L
Sbjct 191 DNLIGTINKQIEVLNKAKKGMMKKLFTKGVFEHKSFKKSEI---GEIPEDWEVVELGNEK 247
Query 253 SEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQEYLLDDLDILLA 312
K+ G + + S +KE + + GN+ L + K + ++DD DIL++
Sbjct 248 YFKIIMGQSPPSSSYNKEGEGVPFLQGKAEFGNIYPNPV--LYTNKPLK-VVDDEDILIS 304
Query 313 RSGATVGKSYLYKAESVGIA----CYAGYLIRARLDQENYNPEFVNYFLQSKQYWDWISS 368
A VG V IA C L + ++E + FV Y+L + I
Sbjct 305 -VRAPVG--------DVNIAPFKLCIGRGLAGIKSNKEKVDNFFVFYYLSYIK--PKIEY 353
Query 369 INIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKLVHLLNEYR 428
+ A + ++ + + + +P LEEQK + + LK D+ I +K + + +
Sbjct 354 LGGGAVFKAITKKDLESIKIPLPPLEEQKAIAKRLKAIDD----LIEIKRKEKEQIEKAK 409
Query 429 STLITQVVTGKIDVQ 443
++ ++TGKI V+
Sbjct 410 KKIMNLLLTGKIRVK 424
>P06187 Type I restriction enzyme StySJI specificity subunit [Salmonella
typhimurium (strain LT2 / SGSC1412 / ATCC 700720)]
Length=469
Score = 60.5 bits (145), Expect = 2e-13
Identities = 72/295 (24%), Positives = 129/295 (44%), Gaps = 23/295 (8%)
Query 158 QNVSAEKYNDLALSIPSLAEQKIIADFLDKRLAQVDALIAKQETLLEKLAEQR----VAL 213
+ V+ E + + +P LAEQK+IA+ LD LAQVD+ A+ E + + L R VA
Sbjct 141 KRVTKEFLENYGVPVPPLAEQKVIAEKLDTLLAQVDSTKARLEQIPQILKRFRQSVIVAA 200
Query 214 ISHAVTKGLNPDVEMKESDVVLLGNIPNTWNIKRLKFLLSEK--LKYGANESAESEDKEN 271
++ +TK L+ + K +++ + +IP+ W I + K + ES +E+
Sbjct 201 VNGQLTKELHKKNKFKLTELNI--SIPSLWKISEIGQFADVKGGKRLPKGESLIAENTGF 258
Query 272 PRYIRITDIDDSGNLKDETFKSLE---SEKAQEYLLDDLDILLARSGATVGKSYLYKAES 328
P YIR + +G + E LE + Y + D+ + GA +G + +
Sbjct 259 P-YIRAGQL-KNGTVLPEGQLYLEEYIQKSISRYTVSSGDLYITIVGACIGDAGIIPDVY 316
Query 329 VGIACYAGYLIRARLDQENYNPEFVNYFLQSKQYWDWISSINIQATIQNVSAEKYNDLTL 388
L++ +N F++ +L+S D I+S ++ + L L
Sbjct 317 NNANLTENAAKICNLNENIFN-RFLSLWLRSSYLQDIINSEIKSGAQGKLALARIKSLPL 375
Query 389 AIPSLEEQ-------KQLIEYLKNEDEKFNRAISKGKKLVH--LLNEYRSTLITQ 434
+P L+EQ +QL Y +++ N A+++ L L +R L Q
Sbjct 376 ILPPLQEQHEIVRRVEQLFAYADTIEKQVNNALTRVNSLTQSILAKAFRGELTAQ 430
Score = 31.2 bits (69), Expect = 4e-04
Identities = 44/201 (22%), Positives = 82/201 (41%), Gaps = 6/201 (3%)
Query 21 IPSHWEVKRMKFLLSEK--LKYGANESAESEDKDQPRYIRITDINDSGTLREDTFKSLE- 77
IPS W++ + K + ES +E+ P YIR + + L E E
Sbjct 224 IPSLWKISEIGQFADVKGGKRLPKGESLIAENTGFP-YIRAGQLKNGTVLPEGQLYLEEY 282
Query 78 IEKA-QEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYLIRARFNKENYDPQFIN 136
I+K+ Y ++ D+ + GA +G + + D N A + EN +F++
Sbjct 283 IQKSISRYTVSSGDLYITIVGACIGDAGI-IPDVYNNANLTENAAKICNLNENIFNRFLS 341
Query 137 LFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKIIADFLDKRLAQVDALI 196
L+L+S I S ++ + L L +P L EQ I +++ A D +
Sbjct 342 LWLRSSYLQDIINSEIKSGAQGKLALARIKSLPLILPPLQEQHEIVRRVEQLFAYADTIE 401
Query 197 AKQETLLEKLAEQRVALISHA 217
+ L ++ ++++ A
Sbjct 402 KQVNNALTRVNSLTQSILAKA 422
>P06991 Type I restriction enzyme EcoDI specificity subunit [Escherichia
coli]
Length=444
Score = 49.3 bits (116), Expect = 8e-10
Identities = 66/323 (20%), Positives = 131/323 (41%), Gaps = 73/323 (23%)
Query 155 ATIQNVSAEKYNDLALSIPSLAEQKIIADFLDKRLAQVDALIAKQETLLEKLAEQRVALI 214
+TI + D+++++PS +EQ +IA+ LD LAQV++ A+ E + + L R A++
Sbjct 113 STIPYIRKGDITDISVALPSPSEQTLIAEKLDTLLAQVESTKARLEQIPQILKRFRQAVL 172
Query 215 SHAVTKGLNPDVEMKESDVVL---------------LGNIPNTWNIKRLKFL--LSEKLK 257
+ A+ L + + ++ L +IPN W+ R + ++ KLK
Sbjct 173 TFAMNGELTKEWRSQNNNPAFFPAEKNSLKQFRNKELPSIPNNWSWMRFDQVADIASKLK 232
Query 258 YGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQEYLLDDLDILLARSGAT 317
+ P I + +++ T K+ + +L+D G T
Sbjct 233 ---------SPLDYPNTIHLA----PNHIESWTGKA----SGYQTILED--------GVT 267
Query 318 VGKSYLYKAE-----------SVGIACYAG------YLIRARLDQENYNPEFVNYFLQSK 360
K Y + V IA + G Y I +++D F+ ++ +
Sbjct 268 SAKHEFYTGQIIYSKIRPYLCKVTIATFDGMCSADMYPINSKID-----THFLFRWMLTN 322
Query 361 QYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQ-------KQLIEYLKNEDEKFNRA 413
+ DW S+ + + ++ + +++ + P L EQ +QL Y +++ N A
Sbjct 323 TFTDWASNAESRTVLPKINQKDLSEIPVPTPPLPEQHEIVRRVEQLFAYADTIEKQVNNA 382
Query 414 ISKGKKLVH--LLNEYRSTLITQ 434
+++ L L +R L Q
Sbjct 383 LARVNNLTQSILAKAFRGELTAQ 405
Score = 26.9 bits (58), Expect = 0.008
Identities = 30/139 (22%), Positives = 59/139 (42%), Gaps = 10/139 (7%)
Query 302 YLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSKQ 361
+ D +LLA +G+ K Y K A Y+I L EF+ + L+
Sbjct 52 WAFDTSAVLLAGNGSFSIKKYTGKFN----AYQRTYVIEPIL----IKTEFLYWLLRGN- 102
Query 362 YWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKLV 421
I+ +TI + D+++A+PS EQ + E L + ++ +++
Sbjct 103 -IKKITENGRGSTIPYIRKGDITDISVALPSPSEQTLIAEKLDTLLAQVESTKARLEQIP 161
Query 422 HLLNEYRSTLITQVVTGKI 440
+L +R ++T + G++
Sbjct 162 QILKRFRQAVLTFAMNGEL 180
Lambda K H a alpha
0.315 0.132 0.372 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 1291626
Database: ac2e046f8e6e03ee3af449964bd01c3d.SwissProt.fasta
Posted date: May 9, 2024 1:16 PM
Number of letters in database: 3,589
Number of sequences in database: 8
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
