BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 9b03a60d3969b7cca467b224cb5de3c2.SwissProt.fasta
2 sequences; 1,945 total letters
Query= ACIAD3357
Length=246
Score E
Sequences producing significant alignments: (Bits) Value
G3XD51 Type IV pilus inner membrane component PilO [Pseudomonas a... 155 5e-51
O76329 Interaptin [Dictyostelium discoideum] 33.1 3e-05
>G3XD51 Type IV pilus inner membrane component PilO [Pseudomonas
aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 /
JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)]
Length=207
Score = 155 bits (392), Expect = 5e-51
Identities = 80/188 (43%), Positives = 116/188 (62%), Gaps = 3/188 (2%)
Query 31 NMLDMSNYGSWPLSVKITCWIFIFFLVCALGYFVVIRPQLDDIQTAQAQEKNLLNEFREK 90
N LD++N GSWP +VK+ + + V ALGY + ++ A+E+ L +F K
Sbjct 16 NDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTK 75
Query 91 QSKLRNLQQYQHQLEEMRASFNQQLTQLPKESEIPGLVEDINMTGVNSGLKFKNIRLENE 150
+ NL+ Y+ Q++EM SF L QLP ++E+PGL+EDI TG+ SGL+F+ I+L E
Sbjct 76 AFQAANLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPE 135
Query 151 VKQEFFIEQPISIEATGDYHAFGAFVSGIAALPRIVTLHDFVIEAKQSKEKTDIPEVDYA 210
V Q+F+IE PI I G YH FVSG+++LPRIVTLHDF I+ + ++ +
Sbjct 136 VAQQFYIELPIQISVVGGYHDLATFVSGVSSLPRIVTLHDFEIKPVAPGSTS---KLRMS 192
Query 211 VKAKTYRY 218
+ AKTYRY
Sbjct 193 ILAKTYRY 200
>O76329 Interaptin [Dictyostelium discoideum]
Length=1738
Score = 33.1 bits (74), Expect = 3e-05
Identities = 28/94 (30%), Positives = 46/94 (49%), Gaps = 5/94 (5%)
Query 66 IRPQLDDIQTAQAQEKNLLNEFREKQSKLR---NLQQYQH--QLEEMRASFNQQLTQLPK 120
I+ L+ + Q ++ L E E+ KL+ N QQ Q QLEE + QL QL +
Sbjct 1321 IQQDLNQLNDDQIKKNEKLKEKEEQLLKLQQDFNDQQSQQLKQLEEKLSEKENQLQQLKQ 1380
Query 121 ESEIPGLVEDINMTGVNSGLKFKNIRLENEVKQE 154
E+EI L + + LK + ++ + + +QE
Sbjct 1381 ENEINQLNQQQQSNEIIQQLKDQLLKQQQQEQQE 1414
Score = 29.3 bits (64), Expect = 4e-04
Identities = 20/86 (23%), Positives = 42/86 (49%), Gaps = 7/86 (8%)
Query 77 QAQEKNLLNEFREKQSKLRNLQQYQHQLEEMRASFNQQLTQLPKESEIPGLVEDINMTGV 136
Q +++ LL++ S N+Q+YQ + ++ FN Q Q + E L + + +
Sbjct 599 QFEKQQLLSQI---DSITTNIQEYQDKFNNLQQEFNTQ--QTLNQQETHRLTQQLYQ--I 651
Query 137 NSGLKFKNIRLENEVKQEFFIEQPIS 162
N+ K +L++E+K I + ++
Sbjct 652 NTDYNEKQTQLQSEIKDNQTINEQLN 677
Score = 28.5 bits (62), Expect = 8e-04
Identities = 23/89 (26%), Positives = 45/89 (51%), Gaps = 9/89 (10%)
Query 73 IQTAQAQEKNLLNEFR--EKQSKLRNLQQYQHQLEEMRASFNQQLTQLPKESEIPGLVED 130
I+ ++ +E+ LN+ + EK+ +L+ LQQ QL E +Q +L E ++ L ++
Sbjct 810 IELSELKEQKELNDSKLIEKEKQLQQLQQEFDQLNEKNQKDHQDQLEL-LEKQLKQLQQE 868
Query 131 INMTGVNSGLKFKNIRLENEVKQEFFIEQ 159
+ L N +EN++ Q+ I +
Sbjct 869 YDQ------LNETNQSIENQLNQQNLINK 891
Score = 28.1 bits (61), Expect = 0.001
Identities = 19/48 (40%), Positives = 28/48 (58%), Gaps = 3/48 (6%)
Query 73 IQTAQAQEKNLLNEFREKQSKLRNLQQYQHQLEEMRASFNQQLTQLPK 120
IQT Q QE + L+ R K +LQQ Q +L++++ SF+ Q Q K
Sbjct 1449 IQTTQ-QEFDQLSHNRSKDQL--HLQQLQQELDQLKQSFDDQDHQFKK 1493
Score = 27.7 bits (60), Expect = 0.001
Identities = 24/92 (26%), Positives = 46/92 (50%), Gaps = 10/92 (11%)
Query 69 QLDDIQTAQAQEKNLLNEFR-EKQSKLRN----LQQYQHQLEEMRASFNQQLTQLPKESE 123
QL+++Q+ Q + N LN+ + EK+ +L++ QY+ Q ++ +QQL +S
Sbjct 754 QLNELQSKQDERFNQLNDEKLEKEKQLQSIEDEFNQYKQQQLSSNSNIDQQL-----QST 808
Query 124 IPGLVEDINMTGVNSGLKFKNIRLENEVKQEF 155
I L E +N + + +++QEF
Sbjct 809 IIELSELKEQKELNDSKLIEKEKQLQQLQQEF 840
Score = 27.7 bits (60), Expect = 0.001
Identities = 21/88 (24%), Positives = 42/88 (48%), Gaps = 9/88 (10%)
Query 80 EKNLLNEFREKQSKLRNLQQYQHQLEEMRASFNQ-------QLTQL--PKESEIPGLVED 130
+ +LL EKQ + + LQ Q + ++++ +FNQ Q QL ++ ++ + +D
Sbjct 1160 QSDLLKLNDEKQQQDKQLQDKQIEFDQLQLTFNQFKNDKDSQFIQLQDDQKQQLQSIQQD 1219
Query 131 INMTGVNSGLKFKNIRLENEVKQEFFIE 158
+N + K K + ++E Q E
Sbjct 1220 LNQLKQENQEKEKQLSEKDEKLQSIQFE 1247
Score = 25.8 bits (55), Expect = 0.005
Identities = 21/86 (24%), Positives = 41/86 (48%), Gaps = 7/86 (8%)
Query 69 QLDDIQTAQAQEKNLLNEFREK-QSKLRNLQQYQHQLEEMRASFNQQLTQLPKESEIPGL 127
+L IQ +++ L+E EK QS +NL Q + +E F++ K+ ++ +
Sbjct 1240 KLQSIQFENQEKEKQLSEKDEKLQSIQQNLNQLNDENQEKVKQFSE------KDEKLQSI 1293
Query 128 VEDINMTGVNSGLKFKNIRLENEVKQ 153
+D+N + K K + ++E Q
Sbjct 1294 QQDLNQLKQENQEKEKQLSEKDEKLQ 1319
Score = 25.0 bits (53), Expect = 0.009
Identities = 34/165 (21%), Positives = 67/165 (41%), Gaps = 38/165 (23%)
Query 3 LDQL-DKTPKESGQPKKKRMNVEKFFQQFNMLDMSNYGSWPLSVKITCWIFIFFLVCALG 61
L+QL D K++ + K+K + K Q FN D + L K++
Sbjct 1325 LNQLNDDQIKKNEKLKEKEEQLLKLQQDFN--DQQSQQLKQLEEKLS------------- 1369
Query 62 YFVVIRPQLDDIQTAQAQEKNLLNEFREKQSKLRNLQQYQHQLEEMRASFNQQLTQLPKE 121
+ Q Q +++N +N+ ++Q +QQ + QL + + Q+ + E
Sbjct 1370 --------EKENQLQQLKQENEINQLNQQQQSNEIIQQLKDQLLKQQ---QQEQQENNNE 1418
Query 122 SEIPGLVEDINMTGVNSGLKFKNIRLENEVKQEFFIEQPISIEAT 166
EI L+++I + ++ + E+ Q + I I+ T
Sbjct 1419 KEIERLIQEI-----------EQLKQQQEIDQSELSNKEIKIQTT 1452
Score = 24.3 bits (51), Expect = 0.016
Identities = 18/77 (23%), Positives = 38/77 (49%), Gaps = 9/77 (12%)
Query 77 QAQEKNLLNEFREKQSKLRNLQQYQHQLEEMRASFNQQLTQLPKESEIPGLVEDINMTGV 136
Q ++NL+N+ NL + + +L +++ NQQ+ ++ + + IN+ V
Sbjct 882 QLNQQNLINK--------ENLNEKEQELLKLQNQLNQQIEKIQFDQQEFSKQNSINIELV 933
Query 137 NSGLKFKNIRLENEVKQ 153
N + K I+L+ + Q
Sbjct 934 NEKNE-KLIQLQQDYDQ 949
Score = 23.9 bits (50), Expect = 0.022
Identities = 26/108 (24%), Positives = 50/108 (46%), Gaps = 14/108 (13%)
Query 69 QLDDIQT---AQAQEKNLLNEFREKQSKLRNLQ-QYQHQLEEMRASFNQQLTQLPKESE- 123
++DDI+ Q Q +++EK KL NL+ + Q + ++ NQ T SE
Sbjct 417 KVDDIEKLTNLQDQLTEQQQQYQEKSLKLVNLELELQEKSNQLVDKSNQLSTMQATNSEL 476
Query 124 ---IPGLVEDINMTGVNSGLKFKNIRLENEVKQEFFIEQPISIEATGD 168
I GL+ D+ + + ++I+ ++E+ IE +++ D
Sbjct 477 MEKIGGLMNDL------TDIPTQDIKEKDEIIANLKIESEKNLKCFQD 518
Score = 23.9 bits (50), Expect = 0.022
Identities = 29/99 (29%), Positives = 49/99 (49%), Gaps = 18/99 (18%)
Query 66 IRPQLDDIQTAQAQEKNLLNEFREKQSKLRNLQQYQHQLEEMRASFNQQLTQLPKESEIP 125
++ ++ D QT Q L+E ++ KL N QQ Q Q E++ N L ++ KE +
Sbjct 662 LQSEIKDNQTINEQLNKQLSEKDKEIEKLSN-QQEQQQDEKI----NNLLLEI-KEKDC- 714
Query 126 GLVEDINMT-----GVNSG-----LKFKNIRLENEVKQE 154
L+E IN +NS L+F+N +L + ++E
Sbjct 715 -LIERINQQLLENIDLNSKYQQLLLEFENFKLNSSKEKE 752
Score = 23.9 bits (50), Expect = 0.022
Identities = 23/63 (37%), Positives = 34/63 (54%), Gaps = 6/63 (10%)
Query 71 DDIQTAQAQEKNLLNEFREKQS-KLRNLQQYQHQLEEMRASFNQQLTQLPKESEIPGLVE 129
+ IQ Q+Q LNE R++QS +L Q +QL E + F+Q+ QL ++S L E
Sbjct 1009 NQIQQLQSQ----LNEQRQQQSNQLSEKDQQLNQLIE-KNQFDQKEQQLKQQSIENDLFE 1063
Query 130 DIN 132
N
Sbjct 1064 KEN 1066
Score = 21.9 bits (45), Expect = 0.088
Identities = 14/52 (27%), Positives = 26/52 (50%), Gaps = 5/52 (10%)
Query 74 QTAQAQE--KNLLNEFREKQSKLRNLQQYQHQLEEMRASFNQQLTQLPKESE 123
QT+Q Q+ K L+NE +E+ K ++ + + A + + QL E +
Sbjct 533 QTSQLQDRIKQLINELQERDDK---FIEFTNSSNQSLADNQRVIDQLTNEKQ 581
Score = 21.6 bits (44), Expect = 0.12
Identities = 13/62 (21%), Positives = 27/62 (44%), Gaps = 0/62 (0%)
Query 182 LPRIVTLHDFVIEAKQSKEKTDIPEVDYAVKAKTYRYIGVDEQGNASAVQANTSTTTPAQ 241
+ ++ L D + E +Q ++ + V+ ++ + VD+ S +QA S
Sbjct 421 IEKLTNLQDQLTEQQQQYQEKSLKLVNLELELQEKSNQLVDKSNQLSTMQATNSELMEKI 480
Query 242 GG 243
GG
Sbjct 481 GG 482
Score = 21.2 bits (43), Expect = 0.15
Identities = 33/135 (24%), Positives = 60/135 (44%), Gaps = 14/135 (10%)
Query 22 NVEKFFQQFNMLDMSNYGSWPLSVKITCWIFIFFLVCALGY---FVVIRPQLDDIQTAQA 78
N++++ +FN L L+ + T + Y ++ ++ D QT
Sbjct 615 NIQEYQDKFNNLQQEFNTQQTLNQQETHRLTQQLYQINTDYNEKQTQLQSEIKDNQTINE 674
Query 79 QEKNLLNEFREKQSKLRNLQQYQHQLEEMRASFNQQLTQLPKESEIPGLVEDIN---MTG 135
Q L+E ++ KL N QQ Q Q E++ N L ++ KE + L+E IN +
Sbjct 675 QLNKQLSEKDKEIEKLSN-QQEQQQDEKI----NNLLLEI-KEKDC--LIERINQQLLEN 726
Query 136 VNSGLKFKNIRLENE 150
++ K++ + LE E
Sbjct 727 IDLNSKYQQLLLEFE 741
Score = 18.9 bits (37), Expect = 0.81
Identities = 28/146 (19%), Positives = 55/146 (38%), Gaps = 40/146 (27%)
Query 4 DQLDKTPKESGQP-----KKKRMNVEKFFQQFNMLDMSNYGSWPLSVKITCWIFIFFLVC 58
+QLD+ KE +P +K+ ++ + L +S L
Sbjct 1513 NQLDQLLKEKLKPLELDSNEKQKTIDDLLSNISNLQISLQNDKDL--------------- 1557
Query 59 ALGYFVVIRPQLDDIQTAQAQEKNLLNEFREKQSKLRNLQQYQHQLEE------------ 106
I + + I+T +++ L+ EK + +++LQQ + Q ++
Sbjct 1558 -------ISERNNSIKTLESRITQQLSLLDEKDNLIKDLQQQKQQQQQPPTASSSPSSSP 1610
Query 107 -MRASFNQQLTQLPKESEIPGLVEDI 131
+ +S Q P + EI LV +I
Sbjct 1611 SLLSSTPTPKPQRPNQIEIDRLVNEI 1636
Score = 16.9 bits (32), Expect = 3.2
Identities = 10/36 (28%), Positives = 20/36 (56%), Gaps = 2/36 (6%)
Query 4 DQLDKTPKESGQPKKKRMNVEKFFQQFN--MLDMSN 37
DQL + ++ + K +N+E Q+ + ++D SN
Sbjct 429 DQLTEQQQQYQEKSLKLVNLELELQEKSNQLVDKSN 464
Score = 16.2 bits (30), Expect = 5.6
Identities = 9/26 (35%), Positives = 12/26 (46%), Gaps = 0/26 (0%)
Query 99 QYQHQLEEMRASFNQQLTQLPKESEI 124
+YQ Q + S Q L K SE+
Sbjct 126 RYQIQSMQNSKSSQQNLHSSTKPSEL 151
Score = 16.2 bits (30), Expect = 5.6
Identities = 11/37 (30%), Positives = 19/37 (51%), Gaps = 1/37 (3%)
Query 176 VSGIAALPRIVTLHDFVIEAKQSKEKTDIPEVDYAVK 212
V I + +IV + ++E ++ E I EV+ VK
Sbjct 382 VEKIIEVEKIVEVEK-IVEVEKIVEVEKIVEVEKIVK 417
Score = 15.4 bits (28), Expect = 9.8
Identities = 13/87 (15%), Positives = 30/87 (34%), Gaps = 0/87 (0%)
Query 82 NLLNEFREKQSKLRNLQQYQHQLEEMRASFNQQLTQLPKESEIPGLVEDINMTGVNSGLK 141
N+ S L N ++ ++ +++ +Q Q ++ + N +
Sbjct 260 NISPSLSSSSSSLLNTPNKRNSIQLSKSTSFEQQNQQQQQQNLLSPNSYRNSISFSKSPS 319
Query 142 FKNIRLENEVKQEFFIEQPISIEATGD 168
F+ + + I PI TG+
Sbjct 320 FEGSQSTGSSRSISPISSPIKNSTTGN 346
Score = 15.4 bits (28), Expect = 9.8
Identities = 6/23 (26%), Positives = 13/23 (57%), Gaps = 1/23 (4%)
Query 208 DYAVKAKTYRYIGVDEQGNASAV 230
DY V + YR + +D+ ++ +
Sbjct 1658 DYIVNSIIYR-LSLDDDNDSDLI 1679
Lambda K H a alpha
0.319 0.135 0.387 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 388752
Database: 9b03a60d3969b7cca467b224cb5de3c2.SwissProt.fasta
Posted date: May 9, 2024 6:27 AM
Number of letters in database: 1,945
Number of sequences in database: 2
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
