BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 69d341b4039c8434c1424060ba4dd90d.SwissProt.fasta
12 sequences; 9,482 total letters
Query= ACIAD3349
Length=474
Score E
Sequences producing significant alignments: (Bits) Value
E1V8I0 Glutamate synthase [NADPH] small chain [Halomonas elongata... 744 0.0
P09832 Glutamate synthase [NADPH] small chain [Escherichia coli (... 678 0.0
P37127 Putative oxidoreductase AegA [Escherichia coli (strain K12)] 582 0.0
Q46820 Putative oxidoreductase UacF [Escherichia coli (strain K12)] 497 8e-175
Q05756 Glutamate synthase [NADPH] small chain [Azospirillum brasi... 374 1e-128
O08340 Putative glutamate synthase [NADPH] small chain [Cereibact... 311 4e-105
Q8U195 Sulfide dehydrogenase subunit alpha [Pyrococcus furiosus (... 292 5e-97
P9WN19 Glutamate synthase [NADPH] small chain [Mycobacterium tube... 263 1e-85
P9WN18 Glutamate synthase [NADPH] small chain [Mycobacterium tube... 263 1e-85
O34399 Glutamate synthase [NADPH] small chain [Bacillus subtilis ... 258 1e-83
Q03460 Glutamate synthase [NADH], amyloplastic [Medicago sativa] 246 1e-74
Q9LV03 Glutamate synthase 1 [NADH], chloroplastic [Arabidopsis th... 246 2e-74
>E1V8I0 Glutamate synthase [NADPH] small chain [Halomonas elongata
(strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198
/ 1H9)]
Length=472
Score = 744 bits (1921), Expect = 0.0
Identities = 351/473 (74%), Positives = 406/473 (86%), Gaps = 1/473 (0%)
Query 1 MAERLNNDFQFLDVARQDPEKKDIDVRKSEFVEIYKPFTAEVAANQTHRCLGCGNPYCEW 60
MA RLNNDFQF+DV RQDPEKK R +F EIY+P+ + AA Q HRCL CGNPYCEW
Sbjct 1 MANRLNNDFQFIDVGRQDPEKKPARTRAKQFAEIYEPYKPQDAAAQAHRCLHCGNPYCEW 60
Query 61 KCPVHNYIPNWLKLIAEGRIFQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAV 120
KCPVHNYIPNWL+L++EG I +AAEL H+TN+LPEVCGRVCPQDRLCEG CTLNDGFGAV
Sbjct 61 KCPVHNYIPNWLQLVSEGNILEAAELSHRTNSLPEVCGRVCPQDRLCEGDCTLNDGFGAV 120
Query 121 TIGNAEKYINDTAFALGWRPDMSSVKWTNKKVAIIGAGPAGLGCADILVRNGVKPVVFDK 180
TIG+ EKYI DTAFA+GWRPDMS V WT+KKVAIIGAGPAGLGCADILVRNGVKPVVFDK
Sbjct 121 TIGSVEKYITDTAFAMGWRPDMSKVTWTDKKVAIIGAGPAGLGCADILVRNGVKPVVFDK 180
Query 181 RPEIGGLLTFGIPEFKMEKDVMKRRREIFTGMGVEFRLNVEIGKDVTIEQLLEDYDAVFM 240
PEIGGLLTFGIPEFK+EK VM+RRR +F MGVEF L VEIG+D+ EQLLE+YDAVF+
Sbjct 181 YPEIGGLLTFGIPEFKLEKTVMERRRAVFEEMGVEFCLGVEIGRDMPFEQLLEEYDAVFL 240
Query 241 GMGTYTYMKGGFPGEDLDGVYDALDFLIANVNRTQGWEQNPSDYISVEGKKVIVLGGGDT 300
GMGTY YM+GGFPGEDL GV+ ALD+L+ANVN G+E +P+DY+S+EG++V+VLGGGDT
Sbjct 241 GMGTYKYMEGGFPGEDLPGVHKALDYLVANVNHCLGFETDPADYVSLEGQRVVVLGGGDT 300
Query 301 AMDCNRTSIRQGAEEVTCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPIEIVGENGQV 360
AMDCNRT+IRQGA VTCAYRRDE NMPGSR+EV NAREEGV+FLFNRQP+ ++GE+ +V
Sbjct 301 AMDCNRTAIRQGAASVTCAYRRDEDNMPGSRKEVANAREEGVDFLFNRQPVAVIGED-RV 359
Query 361 TGVKVVTTQLGAPDSRGRRSPEPIPGSEEVLAADAVLLAFGFRPSPADWFASSSINLDGS 420
G+KVV T+LG PD GR+ PE +PGSEEV+ ADAV++AFGF+PSPA WF + I LD
Sbjct 360 EGIKVVRTRLGEPDENGRQRPEVVPGSEEVVPADAVVIAFGFQPSPAPWFETVGIELDEK 419
Query 421 GRVVAAEQQQYKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYLDV 473
GRV A E+ Y FQT+N KIFAGGDMVRGSDLVVTA++EGR+A EGILDYLDV
Sbjct 420 GRVKAPEEGAYAFQTTNEKIFAGGDMVRGSDLVVTAVFEGRQAGEGILDYLDV 472
>P09832 Glutamate synthase [NADPH] small chain [Escherichia coli
(strain K12)]
Length=472
Score = 678 bits (1749), Expect = 0.0
Identities = 324/469 (69%), Positives = 382/469 (81%), Gaps = 2/469 (0%)
Query 7 NDFQFLDVARQDPEKKDIDVRKSEFVEIYKPFTAEVAANQTHRCLGCGNPYCEWKCPVHN 66
N +QF+D+ R DP KK + +RK EFVEIY+PF+ A Q RCL CGNPYCEWKCPVHN
Sbjct 4 NVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCPVHN 63
Query 67 YIPNWLKLIAEGRIFQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNAE 126
YIPNWLKL EGRIF+AAEL HQTNTLPEVCGRVCPQDRLCEG+CTLND FGAVTIGN E
Sbjct 64 YIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIE 123
Query 127 KYINDTAFALGWRPDMSSVKWTNKKVAIIGAGPAGLGCADILVRNGVKPVVFDKRPEIGG 186
+YIND AF +GWRPDMS VK T KKVAIIGAGPAGL CAD+L RNGVK VVFD+ PEIGG
Sbjct 124 RYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLACADVLTRNGVKAVVFDRHPEIGG 183
Query 187 LLTFGIPEFKMEKDVMKRRREIFTGMGVEFRLNVEIGKDVTIEQLLEDYDAVFMGMGTYT 246
LLTFGIP FK+EK+VM RRREIFTGMG+EF+LN E+G+DV ++ LL DYDAVF+G+GTY
Sbjct 184 LLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLDDLLSDYDAVFLGVGTYQ 243
Query 247 YMKGGFPGEDLDGVYDALDFLIANVNRTQGW-EQNPSDYISVEGKKVIVLGGGDTAMDCN 305
M+GG ED DGVY AL FLIAN + G+ E ++S+EGK+V+VLGGGDTAMDC
Sbjct 244 SMRGGLENEDADGVYAALPFLIANTKQLMGFGETRDEPFVSMEGKRVVVLGGGDTAMDCV 303
Query 306 RTSIRQGAEEVTCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPIEI-VGENGQVTGVK 364
RTS+RQGA+ VTCAYRRDE NMPGSRREV+NAREEGV F FN QP+ I V NG+V+GVK
Sbjct 304 RTSVRQGAKHVTCAYRRDEENMPGSRREVKNAREEGVEFKFNVQPLGIEVNGNGKVSGVK 363
Query 365 VVTTQLGAPDSRGRRSPEPIPGSEEVLAADAVLLAFGFRPSPADWFASSSINLDGSGRVV 424
+V T++G PD++GRR E + GSE ++ ADAV++AFGFRP +W A S+ LD GR++
Sbjct 364 MVRTEMGEPDAKGRRRAEIVAGSEHIVPADAVIMAFGFRPHNMEWLAKHSVELDSQGRII 423
Query 425 AAEQQQYKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYLDV 473
A E FQTSNPKIFAGGD+VRGSDLVVTAI EGR+AA+GI+++L+V
Sbjct 424 APEGSDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNWLEV 472
>P37127 Putative oxidoreductase AegA [Escherichia coli (strain
K12)]
Length=659
Score = 582 bits (1501), Expect = 0.0
Identities = 278/462 (60%), Positives = 349/462 (76%), Gaps = 3/462 (1%)
Query 15 ARQDPEKKDIDVRKSEFVEIYKPFTAEVAANQTHRCLGCG-NPYCEWKCPVHNYIPNWLK 73
AR +P+K I+ RK+ F EIY PF A+ A + RCL CG + CEW CP+HN+IP W++
Sbjct 192 ARGEPDKLAIEARKTGFDEIYLPFRADQAQREASRCLKCGEHSVCEWTCPLHNHIPQWIE 251
Query 74 LIAEGRIFQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNAEKYINDTA 133
L+ G I A EL HQTNTLPE+ GRVCPQDRLCEGACT+ D GAVTIGN E+YI+D A
Sbjct 252 LVKAGNIDAAVELSHQTNTLPEITGRVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQA 311
Query 134 FALGWRPDMSSVKWTNKKVAIIGAGPAGLGCADILVRNGVKPVVFDKRPEIGGLLTFGIP 193
A GWRPD+S V +K+VAIIGAGPAGL CAD+L RNGV V+D+ PEIGGLLTFGIP
Sbjct 312 LAKGWRPDLSHVTKVDKRVAIIGAGPAGLACADVLTRNGVGVTVYDRHPEIGGLLTFGIP 371
Query 194 EFKMEKDVMKRRREIFTGMGVEFRLNVEIGKDVTIEQLLEDYDAVFMGMGTYTYMKGGFP 253
FK++K ++ RRREIF+ MG+ F LN E+GKDV+++ LLE YDAVF+G+GTY MK G P
Sbjct 372 SFKLDKSLLARRREIFSAMGIHFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLP 431
Query 254 GEDLDGVYDALDFLIANVNRTQGWEQNPSD-YISVEGKKVIVLGGGDTAMDCNRTSIRQG 312
ED GVYDAL FLIAN + G E+ P + +I+ G V+VLGGGDTAMDC RT++R G
Sbjct 432 NEDAPGVYDALPFLIANTKQVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHG 491
Query 313 AEEVTCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPIEI-VGENGQVTGVKVVTTQLG 371
A VTCAYRRDEANMPGS++EV+NAREEG NF FN QP+ + + E G V G++ + T+LG
Sbjct 492 ASNVTCAYRRDEANMPGSKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLG 551
Query 372 APDSRGRRSPEPIPGSEEVLAADAVLLAFGFRPSPADWFASSSINLDGSGRVVAAEQQQY 431
PD++GRR P P+ GSE V+ ADAV++AFGF P W S + +D GR++A + QY
Sbjct 552 EPDAQGRRRPVPVEGSEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQY 611
Query 432 KFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYLDV 473
++QT+NPKIFAGGD VRG+DLVVTA+ EGR AA+GI+D+L V
Sbjct 612 RYQTTNPKIFAGGDAVRGADLVVTAMAEGRHAAQGIIDWLGV 653
Score = 21.2 bits (43), Expect = 1.5
Identities = 17/67 (25%), Positives = 27/67 (40%), Gaps = 17/67 (25%)
Query 47 THRCLGCGNPYCEWKCPV-----------HNYIPNWLKLIAEGRIFQAAELCHQTNTLPE 95
+ +CLGC CE C + H++ P + + + ++A CH P
Sbjct 9 SQQCLGC--HACEIACVMAHNDEQHVLSQHHFHPR--ITVIKHQQQRSAVTCHHCEDAP- 63
Query 96 VCGRVCP 102
C R CP
Sbjct 64 -CARSCP 69
>Q46820 Putative oxidoreductase UacF [Escherichia coli (strain
K12)]
Length=639
Score = 497 bits (1279), Expect = 8e-175
Identities = 250/461 (54%), Positives = 315/461 (68%), Gaps = 3/461 (1%)
Query 15 ARQDPEKKDIDVRKSEFVEIYKPFTAEVAANQTHRCLGCGNPY-CEWKCPVHNYIPNWLK 73
+R+ +K RK+ F EIY + A ++ RC+ C C W CP+HN IP++++
Sbjct 175 SRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIR 234
Query 74 LIAEGRIFQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNAEKYINDTA 133
L+ EG+I +AAELCHQT++LPE+CGRVCPQDRLCEGACTL D GAV+IGN E+YI DTA
Sbjct 235 LVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTA 294
Query 134 FALGWRPDMSSVKWTNKKVAIIGAGPAGLGCADILVRNGVKPVVFDKRPEIGGLLTFGIP 193
A+GWRPD+S V ++KVA+IGAGPAGLGCADIL R GV+ VFD+ PEIGG+LTFGIP
Sbjct 295 LAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGVQVDVFDRHPEIGGMLTFGIP 354
Query 194 EFKMEKDVMKRRREIFTGMGVEFRLNVEIGKDVTIEQLLEDYDAVFMGMGTYTYMKGGFP 253
FK++K V+ +RREIFT MG++F LN EIG+D+T L +YDAVF+G+GTY M+ P
Sbjct 355 PFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSEYDAVFIGVGTYGMMRADLP 414
Query 254 GEDLDGVYDALDFLIANVNRTQGW-EQNPSDYISVEGKKVIVLGGGDTAMDCNRTSIRQG 312
ED GV AL FL A+ + G E VEGK+V+VLGGGDT MDC RTSIR
Sbjct 415 HEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLN 474
Query 313 AEEVTCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPIEIV-GENGQVTGVKVVTTQLG 371
A VTCAYRRDE +MPGSR+EV NAREEGV F FN QP I E+G++T V ++ T +G
Sbjct 475 AASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMG 534
Query 372 APDSRGRRSPEPIPGSEEVLAADAVLLAFGFRPSPADWFASSSINLDGSGRVVAAEQQQY 431
P GRR P P+ GSE L AD +++AFGF+ W S I LD G + +
Sbjct 535 EPGPDGRRRPRPVAGSEFELPADVLIMAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYL 594
Query 432 KFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYLD 472
QT K+FAGGD V G+DLVVTA+ GR+AA +L D
Sbjct 595 PTQTHLKKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFD 635
Score = 27.3 bits (59), Expect = 0.019
Identities = 18/69 (26%), Positives = 29/69 (42%), Gaps = 9/69 (13%)
Query 38 FTAEVAANQTHRCLGCGNPYCEWKCPVHNYIPNWLKLIAEGRIFQAAELCHQTNTLPEVC 97
F ++ +C+GC C CP + + IA Q +LC+Q ++ + C
Sbjct 75 FQSDSVQLDEQKCIGCKR--CAIACPFG--VVEMVDTIA-----QKCDLCNQRSSGTQAC 125
Query 98 GRVCPQDRL 106
VCP L
Sbjct 126 IEVCPTQAL 134
Score = 26.6 bits (57), Expect = 0.033
Identities = 22/75 (29%), Positives = 30/75 (40%), Gaps = 19/75 (25%)
Query 44 ANQTHRCLGCGNPYCEWKCPVHNYIPNW----------LKLIAEGRIFQAAE--LCHQTN 91
A + C+GC CE C V + NW + ++ +G QAA CH N
Sbjct 6 AAEAAECIGCHA--CEIACAVAHNQENWPLSHSDFRPRIHVVGKG---QAANPVACHHCN 60
Query 92 TLPEVCGRVCPQDRL 106
P C CP + L
Sbjct 61 NAP--CVTACPVNAL 73
>Q05756 Glutamate synthase [NADPH] small chain [Azospirillum brasilense]
Length=482
Score = 374 bits (959), Expect = 1e-128
Identities = 203/464 (44%), Positives = 280/464 (60%), Gaps = 8/464 (2%)
Query 11 FLDVARQDPEKKDIDVRKSEFVEIYKPFTAEVAANQTHRCLGCGNPYCEWKCPVHNYIPN 70
F+ A++ P+K+ R+ +F EIY F+ E A Q +RC CG P+C+ CPV N IP+
Sbjct 9 FVHTAQRMPDKRPAAERRQDFAEIYARFSDERANEQANRCSQCGVPFCQVHCPVSNNIPD 68
Query 71 WLKLIAEGRIFQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDG-FGAVTIGNAEKYI 129
WLKL +EGR+ +A E+ TN PE+CGR+CPQDRLCEG C + GAVTIG+ EKYI
Sbjct 69 WLKLTSEGRLEEAYEVSQATNNFPEICGRICPQDRLCEGNCVIEQSTHGAVTIGSVEKYI 128
Query 130 NDTAFALGWRPDMSSVKWTNKKVAIIGAGPAGLGCADILVRNGVKPVVFDKRPEIGGLLT 189
NDTA+ GW + + V +IGAGPAGL A+ L G + V+D+ +GGLL
Sbjct 129 NDTAWDQGWVKPRTPSRELGLSVGVIGAGPAGLAAAEELRAKGYEVHVYDRYDRMGGLLV 188
Query 190 FGIPEFKMEKDVMKRRREIFTGMGVEFRLNVEIGKDVTIEQLLEDYDAVFMGMGTYTYMK 249
+GIP FK+EK V++RR ++ GV + N E+G+D ++ +L + AV + G Y
Sbjct 189 YGIPGFKLEKSVVERRVKLLADAGVIYHPNFEVGRDASLPELRRKHVAVLVATGVYKARD 248
Query 250 GGFPGEDLDGVYDALDFLIAN--VNRTQGWEQNPSDYISVEGKKVIVLGGGDTAMDCNRT 307
PG L + ALD+L + V+ E + ++ GK V+VLGGGDTAMDC RT
Sbjct 249 IKAPGSGLGNIVAALDYLTTSNKVSLGDTVEAYENGSLNAAGKHVVVLGGGDTAMDCVRT 308
Query 308 SIRQGAEEVTCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPIEIVGENGQVTGVKVVT 367
+IRQGA V C YRRD NMPGS+REV +A EEGV F++ P G+ VTGV+ V
Sbjct 309 AIRQGATSVKCLYRRDRKNMPGSQREVAHAEEEGVEFIWQAAPEGFTGDT-VVTGVRAVR 367
Query 368 TQLGAPDSRGRRSPEPIPGSEEVLAADAVLLAFGFRPSP-ADWFASSSINLDGSGRVVAA 426
LG D+ GR++P+ I GSE + AD V+ A GF P + F + + G ++
Sbjct 368 IHLGVADATGRQTPQVIEGSEFTVQADLVIKALGFEPEDLPNAFDEPELKVTRWGTLLVD 427
Query 427 EQQQYKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDY 470
+ + T+ +FA GD+VRG+ LVV AI +GR+AAEGI Y
Sbjct 428 HRTK---MTNMDGVFAAGDIVRGASLVVWAIRDGRDAAEGIHAY 468
>O08340 Putative glutamate synthase [NADPH] small chain [Cereibacter
sphaeroides]
Length=413
Score = 311 bits (796), Expect = 4e-105
Identities = 176/380 (46%), Positives = 231/380 (61%), Gaps = 9/380 (2%)
Query 95 EVCGRVCPQDRLCEGACTLND-GFGAVTIGNAEKYINDTAFALGWRPDMSSVKWTNKKVA 153
E+CGR+CPQDRLCEG C + G G VTIG EKYI DTA+ GW ++ V
Sbjct 31 EICGRICPQDRLCEGNCVIEQSGHGTVTIGAVEKYITDTAWENGWVVPGKPAYERSESVG 90
Query 154 IIGAGPAGLGCADILVRNGVKPVVFDKRPEIGGLLTFGIPEFKMEKDVMKRRREIFTGMG 213
IIGAGP GL AD L R G++ V+D+ GGLLT+GIP FK+EKDV+ RR E G
Sbjct 91 IIGAGPGGLAAADALRRAGLQVTVYDRYDRAGGLLTYGIPGFKLEKDVVARRVEQLEQAG 150
Query 214 VEFRLNVEIGKDVTIEQLLEDYDAVFMGMGTYTYMKGGFPGEDLDGVYDALDFLIANVNR 273
V+F LN +G+D++ + + +DAV + G Y PG GV AL +L A+ R
Sbjct 151 VQFVLNCNVGEDLSFDAIRGQHDAVLIATGVYKQRDLAAPGVGSAGVVQALSYLTASNRR 210
Query 274 TQGWEQNPSDYISVEGKKVIVLGGGDTAMDCNRTSIRQGAEEVTCAYRRDEANMPGSRRE 333
+ G ++ D + GK+V+V+GGGDTAMDC RT+IRQGA V C YRRD ANMPGS+RE
Sbjct 211 SFG-DEVDDDGLDASGKRVVVIGGGDTAMDCVRTAIRQGATSVKCLYRRDRANMPGSQRE 269
Query 334 VQNAREEGVNFLFNRQPIEIVGENGQVTGVKVVTTQLGAPDSRGRRSPEPIPGSEEVLAA 393
V NA EEGV F++ P + + V GV V +LG PD+ GR+ PE I G++ V A
Sbjct 270 VANAEEEGVEFVWLSAPRGFIAGDA-VEGVIVQKMRLGEPDATGRQMPEIIEGADYVEPA 328
Query 394 DAVLLAFGFRPS--PADWFASSSINLDGSGRVVAAEQQQYKFQTSNPKIFAGGDMVRGSD 451
D ++A GF P P W + + G + A + TS P ++A GD+VRG+
Sbjct 329 DLAIMALGFEPEDLPTLW-GVPDLTVTRWGTIKADFRTH---ATSLPGVYAVGDIVRGAS 384
Query 452 LVVTAIWEGREAAEGILDYL 471
LVV AI +GR+AA+ ILDYL
Sbjct 385 LVVWAIRDGRDAAQSILDYL 404
>Q8U195 Sulfide dehydrogenase subunit alpha [Pyrococcus furiosus
(strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)]
Length=474
Score = 292 bits (747), Expect = 5e-97
Identities = 170/465 (37%), Positives = 258/465 (55%), Gaps = 21/465 (5%)
Query 21 KKDIDVRKSEFVEIYKPFTAEVAANQTHRCLGCGNPY--CEWKCPVHNYIPNWLKLIAEG 78
++ + R +F E+ ++ E+A + RCL C Y C CPVH IP ++K + E
Sbjct 13 ERSVGERVRDFGEVNLGYSWELALREAERCLQCPVEYAPCIKGCPVHINIPGFIKALREN 72
Query 79 R------IFQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNAEKYINDT 132
R + +A + + NTLP + GRVCPQ+ CEGAC + + IG E+++ D
Sbjct 73 RDNPSKAVREALRIIWRDNTLPAITGRVCPQEEQCEGACVVGKVGDPINIGKLERFVADY 132
Query 133 AFALGWRPDM-----SSVKWTNKKVAIIGAGPAGLGCADILVRNGVKPVVFDKRPEIGGL 187
A G ++ +K KKVAIIGAGPAGL CA L + G + +++ + GG+
Sbjct 133 AREHGIDDELLLEEIKGIKRNGKKVAIIGAGPAGLTCAADLAKMGYEVTIYEALHQPGGV 192
Query 188 LTFGIPEFKMEKDVMKRRREIFTGMGVEFRLNVEIGKDVTIEQLLEDYDAVFMGMGTYTY 247
L +GIPEF++ K+++K+ E +GV+ NV +GK +T E+L E+YDA+F+G G T
Sbjct 193 LIYGIPEFRLPKEIVKKELENLRRLGVKIETNVLVGKTITFEELREEYDAIFIGTGAGTP 252
Query 248 MKGGFPGEDLDGVYDALDFLIANVNRTQGWEQNPSDYISVEGKKVIVLGGGDTAMDCNRT 307
+PG +L+G+Y A +FL +N + ++ D GK+V V+GGG+TAMD R+
Sbjct 253 RIYPWPGVNLNGIYSANEFL-TRINLMKAYKFPEYDTPIKVGKRVAVIGGGNTAMDAARS 311
Query 308 SIRQGAEEVTCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPIEIVG-ENGQVTGVKVV 366
++R GA EV YRR M E+++A EEGV F+F P +G ENG + +++
Sbjct 312 ALRLGA-EVWILYRRTRKEMTAREEEIKHAEEEGVKFMFLVTPKRFIGDENGNLKAIELE 370
Query 367 TTQLGAPDSRGRRSPEPIPGSEEVLAADAVLLAFGFRPSPADWFASSSINLDGSGRVVAA 426
+LG PD GRR P P G ++ D ++A G P+ + +D GR+V
Sbjct 371 KMKLGEPDESGRRRPIP-TGETFIMEFDTAIIAIGQTPNKTFLETVPGLKVDEWGRIVVD 429
Query 427 EQQQYKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYL 471
E TS P +FAGGD +RG V+ A+ +GR+AA+ I YL
Sbjct 430 E----NLMTSIPGVFAGGDAIRGEATVILAMGDGRKAAKAIHQYL 470
>P9WN19 Glutamate synthase [NADPH] small chain [Mycobacterium
tuberculosis (strain ATCC 25618 / H37Rv)]
Length=488
Score = 263 bits (671), Expect = 1e-85
Identities = 173/474 (36%), Positives = 236/474 (50%), Gaps = 34/474 (7%)
Query 16 RQDPEKKDIDVRKSEFVEIYKPFTAEVAANQTHRCLGCGNPYCEWKCPVHNYIPNWLKLI 75
R+ P+++ + +R ++ E+Y+ F E Q RC+ CG P+C CP+ N IP W L+
Sbjct 13 RKLPKRRPVPLRLRDWREVYEEFDNESLRQQATRCMDCGIPFCHNGCPLGNLIPEWNDLV 72
Query 76 AEGRIFQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNAEKYINDTAFA 135
GR A E H TN P+ GR+CP CE AC L VTI E I D AF
Sbjct 73 RRGRWRDAIERLHATNNFPDFTGRLCPAP--CEPACVLGINQDPVTIKQIELEIIDKAFD 130
Query 136 LGWRPDMSSVKWTNKKVAIIGAGPAGLGCADILVRNGVKPVVFDKRPEIGGLLTFGIPEF 195
GW K T + VA++G+GPAGL A L R G VF++ IGGLL +GIPEF
Sbjct 131 EGWVQPRPPRKLTGQTVAVVGSGPAGLAAAQQLTRAGHTVTVFEREDRIGGLLRYGIPEF 190
Query 196 KMEKDVMKRRREIFTGMGVEFRLNVEIGKDVTIEQLLEDYDAVFMGMGTYTYMKGGFPGE 255
KMEK + RR + G EFR V +G D++ E+L D+DAV + G + + PG
Sbjct 191 KMEKRHLDRRLDQMRSEGTEFRPGVNVGVDISAEKLRADFDAVVLAGGATAWRELPIPGR 250
Query 256 DLDGVYDALDFLIANVNRTQG----WEQNPSDYISVEGKKVIVLGGGDTAMDCNRTSIRQ 311
+L+GV+ A++FL NR Q +++ I+ +GKKV+++GGGDT DC T RQ
Sbjct 251 ELEGVHQAMEFL-PWANRVQEGDDVLDEDGQPPITAKGKKVVIIGGGDTGADCLGTVHRQ 309
Query 312 GAEEVTCAYRRDEANMPGSRRE------------VQNAREEGVNFLFNRQPIEIVGENGQ 359
GA + P +R E V A EEG +F+ VG +G+
Sbjct 310 GA--IAVHQFEIMPRPPDARAESTPWPTYPLMYRVSAAHEEGGERVFSVNTEAFVGTDGR 367
Query 360 VTGVKV-VTTQLGAPDSRGRRSPEPIPGSEEVLAADAVLLAFGF-RPSPADWFASSSINL 417
V+ ++ T L + + GS+ L AD VLLA GF P A +
Sbjct 368 VSALRAHEVTMLDGKFVK-------VEGSDFELEADLVLLAMGFVGPERAGLLTDLGVKF 420
Query 418 DGSGRVVAAEQQQYKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYL 471
G V + F TS P +F GDM RG L+V AI EGR AA + YL
Sbjct 421 TERGNVARGDD----FDTSVPGVFVAGDMGRGQSLIVWAIAEGRAAAAAVDRYL 470
>P9WN18 Glutamate synthase [NADPH] small chain [Mycobacterium
tuberculosis (strain CDC 1551 / Oshkosh)]
Length=488
Score = 263 bits (671), Expect = 1e-85
Identities = 173/474 (36%), Positives = 236/474 (50%), Gaps = 34/474 (7%)
Query 16 RQDPEKKDIDVRKSEFVEIYKPFTAEVAANQTHRCLGCGNPYCEWKCPVHNYIPNWLKLI 75
R+ P+++ + +R ++ E+Y+ F E Q RC+ CG P+C CP+ N IP W L+
Sbjct 13 RKLPKRRPVPLRLRDWREVYEEFDNESLRQQATRCMDCGIPFCHNGCPLGNLIPEWNDLV 72
Query 76 AEGRIFQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNAEKYINDTAFA 135
GR A E H TN P+ GR+CP CE AC L VTI E I D AF
Sbjct 73 RRGRWRDAIERLHATNNFPDFTGRLCPAP--CEPACVLGINQDPVTIKQIELEIIDKAFD 130
Query 136 LGWRPDMSSVKWTNKKVAIIGAGPAGLGCADILVRNGVKPVVFDKRPEIGGLLTFGIPEF 195
GW K T + VA++G+GPAGL A L R G VF++ IGGLL +GIPEF
Sbjct 131 EGWVQPRPPRKLTGQTVAVVGSGPAGLAAAQQLTRAGHTVTVFEREDRIGGLLRYGIPEF 190
Query 196 KMEKDVMKRRREIFTGMGVEFRLNVEIGKDVTIEQLLEDYDAVFMGMGTYTYMKGGFPGE 255
KMEK + RR + G EFR V +G D++ E+L D+DAV + G + + PG
Sbjct 191 KMEKRHLDRRLDQMRSEGTEFRPGVNVGVDISAEKLRADFDAVVLAGGATAWRELPIPGR 250
Query 256 DLDGVYDALDFLIANVNRTQG----WEQNPSDYISVEGKKVIVLGGGDTAMDCNRTSIRQ 311
+L+GV+ A++FL NR Q +++ I+ +GKKV+++GGGDT DC T RQ
Sbjct 251 ELEGVHQAMEFL-PWANRVQEGDDVLDEDGQPPITAKGKKVVIIGGGDTGADCLGTVHRQ 309
Query 312 GAEEVTCAYRRDEANMPGSRRE------------VQNAREEGVNFLFNRQPIEIVGENGQ 359
GA + P +R E V A EEG +F+ VG +G+
Sbjct 310 GA--IAVHQFEIMPRPPDARAESTPWPTYPLMYRVSAAHEEGGERVFSVNTEAFVGTDGR 367
Query 360 VTGVKV-VTTQLGAPDSRGRRSPEPIPGSEEVLAADAVLLAFGF-RPSPADWFASSSINL 417
V+ ++ T L + + GS+ L AD VLLA GF P A +
Sbjct 368 VSALRAHEVTMLDGKFVK-------VEGSDFELEADLVLLAMGFVGPERAGLLTDLGVKF 420
Query 418 DGSGRVVAAEQQQYKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAEGILDYL 471
G V + F TS P +F GDM RG L+V AI EGR AA + YL
Sbjct 421 TERGNVARGDD----FDTSVPGVFVAGDMGRGQSLIVWAIAEGRAAAAAVDRYL 470
>O34399 Glutamate synthase [NADPH] small chain [Bacillus subtilis
(strain 168)]
Length=493
Score = 258 bits (659), Expect = 1e-83
Identities = 169/488 (35%), Positives = 243/488 (50%), Gaps = 35/488 (7%)
Query 11 FLDVARQDPEKKDIDVRKSEFVEIYKPFTAEVAANQTHRCLGCGNPYCEWK--------- 61
F+++ R+ P ++D R ++ E PF+ E + Q RC+ CG P+C+
Sbjct 7 FMEIKREKPAERDPLTRLKDWKEYSAPFSEEASKRQGARCMDCGTPFCQIGADINGFTSG 66
Query 62 CPVHNYIPNWLKLIAEGRIFQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVT 121
CP++N IP W L+ GR +A E +TN PE GRVCP CEG+CTL AV+
Sbjct 67 CPIYNLIPEWNDLVYRGRWKEALERLLKTNNFPEFTGRVCPAP--CEGSCTLAISDPAVS 124
Query 122 IGNAEKYINDTAFALGWRPDMSSVKWTNKKVAIIGAGPAGLGCADILVRNGVKPVVFDKR 181
I N E+ I D F GW K T KKVAI+G+GPAGL AD L + G VF++
Sbjct 125 IKNIERTIIDKGFENGWIQPRIPKKRTGKKVAIVGSGPAGLASADQLNQAGHSVTVFERA 184
Query 182 PEIGGLLTFGIPEFKMEKDVMKRRREIFTGMGVEFRLNVEIGKDVTIEQLLEDYDAVFMG 241
GGLLT+GIP K+EK +++RR ++ T G++F N EIG D+T ++L E +DAV +
Sbjct 185 DRAGGLLTYGIPNMKLEKGIVERRIKLLTQEGIDFVTNTEIGVDITADELKEQFDAVILC 244
Query 242 MGTYTYMKGGFPGEDLDGVYDALDFLIANVNRTQGWEQNPSDYISVEGKKVIVLGGGDTA 301
G G D GV+ A+D+L +I +GK VIV+GGGDT
Sbjct 245 TGAQKQRDLLIEGRDSKGVHYAMDYLTLATKSYLDSNFKDKQFIDAKGKDVIVIGGGDTG 304
Query 302 MDCNRTSIRQGAEEV-------TCAYRRDEANMPGSRREV---QNAREEGVNFLFNRQPI 351
DC T++RQ A+ V R NM + V + A EE F R P
Sbjct 305 ADCVATALRQKAKSVHQFGKHPKLPPARTNDNMWPEQPHVFTLEYAYEE-AEAKFGRDPR 363
Query 352 E--------IVGENGQVTGVKVVTTQLGAPDSRGRRSPEPIPGSEEVLAADAVLLAFGFR 403
E + +NG++ + + + + G+ +PG+E+V A V +A GF
Sbjct 364 EYSIQTTKMVADKNGKLKELHTIQME-KVKNEHGKYEFRELPGTEKVWPAQLVFIAIGFE 422
Query 404 PSPADWFASSSINLDGSGRVVAAEQQQYKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREA 463
+ +N + ++ AA +QT+ +FA GD RG L+V AI EGRE
Sbjct 423 GTEQPLLKQFGVN-SVNNKISAAYGD---YQTNIDGVFAAGDARRGQSLIVWAINEGREV 478
Query 464 AEGILDYL 471
A + YL
Sbjct 479 AREVDRYL 486
>Q03460 Glutamate synthase [NADH], amyloplastic [Medicago sativa]
Length=2194
Score = 246 bits (629), Expect = 1e-74
Identities = 173/487 (36%), Positives = 244/487 (50%), Gaps = 34/487 (7%)
Query 11 FLDVARQDPEKKDIDVRKSEFVEIY---KPFTAEVAANQTHRCLGCGNPYCEWK---CPV 64
F+ R+ + +D +VR +++ E+ KP + Q+ RC+ CG P+C + CP+
Sbjct 1684 FVAYEREGVQYRDPNVRLNDWNEVMMETKP--GPLLKTQSARCMDCGTPFCHQENSGCPL 1741
Query 65 HNYIPNWLKLIAEGRIFQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGN 124
N IP + +L+ + R +A E +TN PE GRVCP CEG+C L V+I N
Sbjct 1742 GNKIPEFNELVYQNRWQEALERLLETNNFPEFTGRVCPAP--CEGSCVLGIIENPVSIKN 1799
Query 125 AEKYINDTAFALGWRPDMSSVKWTNKKVAIIGAGPAGLGCADILVRNGVKPVVFDKRPEI 184
E I D AF GW VK T K+VAI+G+GP+GL AD L + G VF++ I
Sbjct 1800 IECAIIDKAFEEGWMIPRPPVKRTGKRVAIVGSGPSGLAAADQLNKMGHIVTVFERADRI 1859
Query 185 GGLLTFGIPEFKMEK-DVMKRRREIFTGMGVEFRLNVEIGKD--VTIEQLLEDYDAVFMG 241
GGL+ +G+P K +K D+++RR + G+ F +N IG D ++E+L E+ DA+ +
Sbjct 1860 GGLMMYGVPNMKTDKVDIVQRRVNLMAEEGINFVVNANIGLDPLYSLERLREENDAIVLA 1919
Query 242 MGTYTYMKGGFPGEDLDGVYDALDFLIANVNRTQGWEQNPSDYISVEGKKVIVLGGGDTA 301
+G PG +L GV+ A++FL AN +YIS +GKKV+V+GGGDT
Sbjct 1920 VGATKPRDLPVPGRELSGVHFAMEFLHANTKSLLDSNLQDGNYISAKGKKVVVIGGGDTG 1979
Query 302 MDCNRTSIRQGAEEV-----------TCAYRRDEANMPGSRREVQNAREEGVNFLFNRQP 350
DC TSIR G V T A P R +E F + +
Sbjct 1980 TDCIGTSIRHGCTAVVNLELLPQPPPTRAPGNPWPQWPRIFRVDYGHQEAETKFGKDPRT 2039
Query 351 IEIV------GENGQVTGVKVVTTQLGAPDSRGRRSPEPIPGSEEVLAADAVLLAFGFRP 404
E++ ENG V G++VV D G+ + I GSEE++ AD VLLA GF
Sbjct 2040 YEVLTKRFVGDENGVVKGLEVVRV-CWEKDETGKFQFKEIEGSEEIIEADLVLLAMGFLG 2098
Query 405 SPADWFASSSINLDGSGRVVAAEQQQYKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAA 464
A + D A +F TS +FA GD RG LVV AI EGR+AA
Sbjct 2099 PEATIAEKLGVERDNRSNFKA---DYGRFSTSVDGVFAAGDCRRGQSLVVWAISEGRQAA 2155
Query 465 EGILDYL 471
+ YL
Sbjct 2156 AQVDSYL 2162
Score = 20.0 bits (40), Expect = 3.7
Identities = 5/16 (31%), Positives = 11/16 (69%), Gaps = 0/16 (0%)
Query 349 QPIEIVGENGQVTGVK 364
QP ++G NG++ ++
Sbjct 327 QPFRVLGHNGEINTLR 342
>Q9LV03 Glutamate synthase 1 [NADH], chloroplastic [Arabidopsis
thaliana]
Length=2208
Score = 246 bits (627), Expect = 2e-74
Identities = 169/486 (35%), Positives = 250/486 (51%), Gaps = 32/486 (7%)
Query 11 FLDVARQDPEKKDIDVRKSEFVEIYKPFT-AEVAANQTHRCLGCGNPYCEWK---CPVHN 66
F+ R+ + +D +VR +++ E+ + + Q+ RC+ CG P+C + CP+ N
Sbjct 1705 FIAYEREGVKYRDPNVRLNDWNEVMEESKPGPLLTTQSARCMDCGTPFCHQENSGCPLGN 1764
Query 67 YIPNWLKLIAEGRIFQAAELCHQTNTLPEVCGRVCPQDRLCEGACTLNDGFGAVTIGNAE 126
IP + +L+ + R +A +TN PE GRVCP CEG+C L V+I + E
Sbjct 1765 KIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAP--CEGSCVLGIIENPVSIKSIE 1822
Query 127 KYINDTAFALGWRPDMSSVKWTNKKVAIIGAGPAGLGCADILVRNGVKPVVFDKRPEIGG 186
I D AF GW +K T KKVAIIG+GPAGL AD L + G V+++ IGG
Sbjct 1823 CAIIDKAFEEGWMVPRPPLKRTGKKVAIIGSGPAGLAAADQLNKMGHLVTVYERSDRIGG 1882
Query 187 LLTFGIPEFKMEK-DVMKRRREIFTGMGVEFRLNVEIGKD--VTIEQLLEDYDAVFMGMG 243
L+ +G+P K +K DV++RR ++ T G+ F +N IGKD +++ L E+ DA+ + +G
Sbjct 1883 LMMYGVPNMKTDKIDVVQRRVDLMTKEGINFVVNANIGKDPSYSLDGLKEENDAIVLAVG 1942
Query 244 TYTYMKGGFPGEDLDGVYDALDFLIANVNRTQGWEQNPSDYISVEGKKVIVLGGGDTAMD 303
+ PG DL GV+ A++FL AN +YIS +GKKV+V+GGGDT D
Sbjct 1943 STKPRDLPVPGRDLSGVHFAMEFLHANTKSLLDSNHEDGNYISAKGKKVVVIGGGDTGTD 2002
Query 304 CNRTSIRQGAEEV-----------TCAYRRDEANMPGSRREVQNAREEGVNFLFNRQPIE 352
C TSIR G + T A P R +E F + + E
Sbjct 2003 CIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQEATTKFGKDPRTYE 2062
Query 353 IV------GENGQVTGVKVVTTQLGAPDSRGRRSPEPIPGSEEVLAADAVLLAFGF-RPS 405
++ +NG V G+++V D GR + I GSEE++ AD V LA GF P
Sbjct 2063 VLTKRFIGDDNGNVKGLELVRVS-WEKDETGRFQFKEIEGSEEIIEADLVFLAMGFLGPE 2121
Query 406 PADWFASSSINLDGSGRVVAAEQQQYKFQTSNPKIFAGGDMVRGSDLVVTAIWEGREAAE 465
P + + L+ R + + +F T+ +FA GD RG LVV AI EGR+AA+
Sbjct 2122 PT---LAEKLGLECDNR-SNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWAISEGRQAAD 2177
Query 466 GILDYL 471
+ +L
Sbjct 2178 QVDKFL 2183
Score = 20.4 bits (41), Expect = 2.8
Identities = 5/16 (31%), Positives = 12/16 (75%), Gaps = 0/16 (0%)
Query 349 QPIEIVGENGQVTGVK 364
QP+ ++G NG++ ++
Sbjct 345 QPMRVLGHNGEINTLR 360
Lambda K H a alpha
0.320 0.139 0.426 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 3710280
Database: 69d341b4039c8434c1424060ba4dd90d.SwissProt.fasta
Posted date: May 20, 2024 6:44 AM
Number of letters in database: 9,482
Number of sequences in database: 12
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
