BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 408d203216b615d6b82f9c6ae426bbdf.SwissProt.fasta
16 sequences; 4,860 total letters
Query= ACIAD3340
Length=288
Score E
Sequences producing significant alignments: (Bits) Value
Q5R4L6 Omega-amidase NIT2 [Pongo abelii] 98.6 1e-27
Q9NQR4 Omega-amidase NIT2 [Homo sapiens] 98.2 2e-27
Q557J5 Deaminated glutathione amidase [Dictyostelium discoideum] 97.1 6e-27
Q9JHW2 Omega-amidase NIT2 [Mus musculus] 94.0 7e-26
Q497B0 Omega-amidase NIT2 [Rattus norvegicus] 94.0 7e-26
Q9UYV8 Nitrilase [Pyrococcus abyssi (strain GE5 / Orsay)] 87.8 1e-23
Q2T9R6 Omega-amidase NIT2 [Bos taurus] 87.0 2e-23
Q28IE5 Omega-amidase NIT2 [Xenopus tropicalis] 85.9 6e-23
Q32LH4 Deaminated glutathione amidase [Bos taurus] 86.7 6e-23
B9IW18 Formamidase [Bacillus cereus (strain Q1)] 86.3 9e-23
P49954 Omega-amidase NIT3 [Saccharomyces cerevisiae (strain ATCC ... 84.3 3e-22
Q8VDK1 Deaminated glutathione amidase [Mus musculus] 84.7 3e-22
Q86X76 Deaminated glutathione amidase [Homo sapiens] 83.2 1e-21
Q8RUF8 Omega-amidase, chloroplastic [Arabidopsis thaliana] 76.6 3e-19
O25836 Formamidase [Helicobacter pylori (strain ATCC 700392 / 266... 75.9 4e-19
Q9L543 Aliphatic amidase [Bacillus sp] 75.5 6e-19
>Q5R4L6 Omega-amidase NIT2 [Pongo abelii]
Length=275
Score = 98.6 bits (244), Expect = 1e-27
Identities = 81/267 (30%), Positives = 135/267 (51%), Gaps = 34/267 (13%)
Query 2 LSNFGIAGIQMQVSAFTS-NVEQMGNYMRHIRMRYPWVKMVLFSEL--APLGPKH--STA 56
+++F +A IQ+Q+S+ S NV + +++R + K+V E +P G K+ A
Sbjct 1 MASFRLALIQLQISSINSDNVTRACSFIREAATQ--GAKIVSLPECFNSPYGTKYFPEYA 58
Query 57 EVFPSQTEAQLAELARETGLWLIPGSLFERVESPEGDV--IYNTLSVINPQGQIVARYRK 114
E P ++ +L+E+A+E ++LI GS+ PE D +YNT +V P G ++A+YRK
Sbjct 59 EKIPGESTQKLSEVAKECSIYLIGGSI------PEEDAGKLYNTCAVFGPDGTLLAKYRK 112
Query 115 MFPFR---------PYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLVAMGAE 165
+ F K+++ G+ FC FD R G+ ICYDM F E + G +
Sbjct 113 IHLFDIDVPGKITFQESKTLSPGDSFCTFDT--YCRVGLGICYDMRFAELAQIYAQRGCQ 170
Query 166 VILHPTMTD--TIDRDIELSIARASAAQNQVYFIDINGVGDG-----GIGRSIVIDPSGY 218
++++P + T EL + R A NQVY + D G S V++P G
Sbjct 171 LLVYPGAFNLTTGPAHWEL-LQRGRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGE 229
Query 219 VLHQSEAGPEIIPIDVDLEKVRRERRR 245
VL ++ I+ D+DL+K+ R++
Sbjct 230 VLAKAGTEEAIVYSDIDLKKLAEIRQQ 256
>Q9NQR4 Omega-amidase NIT2 [Homo sapiens]
Length=276
Score = 98.2 bits (243), Expect = 2e-27
Identities = 81/267 (30%), Positives = 136/267 (51%), Gaps = 33/267 (12%)
Query 2 LSNFGIAGIQMQVSAFTS-NVEQMGNYMRHIRMRYPWVKMVLFSEL--APLGPKH--STA 56
+++F +A IQ+Q+S+ S NV + +++R + K+V E +P G K+ A
Sbjct 1 MTSFRLALIQLQISSIKSDNVTRACSFIREAATQ--GAKIVSLPECFNSPYGAKYFPEYA 58
Query 57 EVFPSQTEAQLAELARETGLWLIPGSLFERVESPEGDV--IYNTLSVINPQGQIVARYRK 114
E P ++ +L+E+A+E ++LI GS+ PE D +YNT +V P G ++A+YRK
Sbjct 59 EKIPGESTQKLSEVAKECSIYLIGGSI------PEEDAGKLYNTCAVFGPDGTLLAKYRK 112
Query 115 MFPFR---------PYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLVAMGAE 165
+ F K+++ G+ F FD P R G+ ICYDM F E + G +
Sbjct 113 IHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYC-RVGLGICYDMRFAELAQIYAQRGCQ 171
Query 166 VILHPTMTD--TIDRDIELSIARASAAQNQVYFIDINGVGDG-----GIGRSIVIDPSGY 218
++++P + T EL + R+ A NQVY + D G S V++P G
Sbjct 172 LLVYPGAFNLTTGPAHWEL-LQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGE 230
Query 219 VLHQSEAGPEIIPIDVDLEKVRRERRR 245
VL ++ I+ D+DL+K+ R++
Sbjct 231 VLAKAGTEEAIVYSDIDLKKLAEIRQQ 257
>Q557J5 Deaminated glutathione amidase [Dictyostelium discoideum]
Length=291
Score = 97.1 bits (240), Expect = 6e-27
Identities = 67/189 (35%), Positives = 98/189 (52%), Gaps = 20/189 (11%)
Query 69 ELARETGLWLIPGSLFERVESPEGDVIYNTLSVINPQGQIVARYRKMFPF---------- 118
+LA++ +WL G E++ D+IYNT +I+ G IV YRKM F
Sbjct 85 DLAKQNNIWLSLGGFHEKILDDPNDMIYNTHLIIDSNGVIVCEYRKMHLFDVDIPSKGVK 144
Query 119 RPYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLVAMGAEVILHPT--MTDTI 176
K V GGN+ V D P VG+ G+SICYD+ FPE +L M A+++L P+ M T
Sbjct 145 MNESKVVKGGNDLVVCDSP-VGKLGLSICYDLRFPELYLSLRRMDAQILLVPSAFMKSTG 203
Query 177 DRDIELSIARASAAQNQVYFIDINGVGD-----GGIGRSIVIDPSGYVLHQ-SEAGPEII 230
+ + + +A A +NQ Y I GD G S++IDP G VLH + +I
Sbjct 204 EAHWK-PLLQARAIENQTYVIAAAQTGDHHSKRSSYGHSMIIDPWGKVLHDLPDNLNDIA 262
Query 231 PIDVDLEKV 239
+D+DL+ +
Sbjct 263 FVDIDLDYI 271
>Q9JHW2 Omega-amidase NIT2 [Mus musculus]
Length=276
Score = 94.0 bits (232), Expect = 7e-26
Identities = 78/265 (29%), Positives = 131/265 (49%), Gaps = 29/265 (11%)
Query 2 LSNFGIAGIQMQVSAFTS-NVEQMGNYMRHIRMRYPWVKMVLFSELAPLGPKH--STAEV 58
+S F +A IQ+QVS+ S N+ + + +R + + + +P G + AE
Sbjct 1 MSTFRLALIQLQVSSIKSDNLTRACSLVREAAKQGANIVSLPECFNSPYGTTYFPDYAEK 60
Query 59 FPSQTEAQLAELARETGLWLIPGSLFERVESPEGDV--IYNTLSVINPQGQIVARYRKMF 116
P ++ +L+E+A+E+ ++LI GS+ PE D +YNT SV P G ++ ++RK+
Sbjct 61 IPGESTQKLSEVAKESSIYLIGGSI------PEEDAGKLYNTCSVFGPDGSLLVKHRKIH 114
Query 117 PFR---------PYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLVAMGAEVI 167
F K+++ G+ F FD P + G+ ICYDM F E + G +++
Sbjct 115 LFDIDVPGKITFQESKTLSPGDSFSTFDTPYC-KVGLGICYDMRFAELAQIYAQRGCQLL 173
Query 168 LHPTMTD--TIDRDIELSIARASAAQNQVYFIDINGVGDG-----GIGRSIVIDPSGYVL 220
++P + T EL + RA A NQVY + D G S V+DP G VL
Sbjct 174 VYPGAFNLTTGPAHWEL-LQRARAVDNQVYVATASPARDDKASYVAWGHSTVVDPWGQVL 232
Query 221 HQSEAGPEIIPIDVDLEKVRRERRR 245
++ I+ D+DL+K+ R++
Sbjct 233 TKAGTEETILYSDIDLKKLAEIRQQ 257
>Q497B0 Omega-amidase NIT2 [Rattus norvegicus]
Length=276
Score = 94.0 bits (232), Expect = 7e-26
Identities = 78/264 (30%), Positives = 130/264 (49%), Gaps = 27/264 (10%)
Query 2 LSNFGIAGIQMQVSAFTS-NVEQMGNYMRHIRMRYPWVKMVLFSELAPLGPKH--STAEV 58
+S F +A IQ+QVS+ S N+ + + +R + + + +P G + AE
Sbjct 1 MSTFRLALIQLQVSSIKSDNITRACSLVREAAKQGANIVSLPECFNSPYGTNYFPEYAEK 60
Query 59 FPSQTEAQLAELARETGLWLIPGSLFERVESPEGDVIYNTLSVINPQGQIVARYRKMFPF 118
P ++ +L+E+A+E ++LI GS+ E + +YNT +V P G ++ ++RK+ F
Sbjct 61 IPGESTKKLSEVAKENSIYLIGGSIPEEDDGK----LYNTCAVFGPDGNLLVKHRKIHLF 116
Query 119 R---------PYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLVAMGAEVILH 169
K+++ G+ F FD P R G+ ICYDM F E + G +++++
Sbjct 117 DIDVPGKITFQESKTLSPGDSFSTFDTPYC-RVGLGICYDMRFAELAQIYARRGCQLLVY 175
Query 170 P---TMTDTIDRDIELSIARASAAQNQVYFIDINGVGDG-----GIGRSIVIDPSGYVLH 221
P MT T EL + RA A NQVY + D G S V+DP G VL
Sbjct 176 PGAFNMT-TGPAHWEL-LQRARAVDNQVYVATASPARDEKASYVAWGHSTVVDPWGQVLT 233
Query 222 QSEAGPEIIPIDVDLEKVRRERRR 245
++ I+ D+DL+K+ R++
Sbjct 234 KAGTEETILYSDIDLKKLSEIRQQ 257
>Q9UYV8 Nitrilase [Pyrococcus abyssi (strain GE5 / Orsay)]
Length=262
Score = 87.8 bits (216), Expect = 1e-23
Identities = 71/236 (30%), Positives = 116/236 (49%), Gaps = 30/236 (13%)
Query 39 KMVLFSELAPLGPKHSTAE--------VFPSQTEAQLAELARETGLWLIPGSLFERVESP 90
++V+ EL G T E + +T L ++AR+TG++++ G+
Sbjct 36 QLVVLPELFDTGYNFETREEVFEIAQKIPEGETTTFLMDVARDTGVYIVAGTA-----EK 90
Query 91 EGDVIYNTLSVINPQGQIVARYRKMFPFRPYEKSVAGGN-EFCVFDVPEVGRFGVSICYD 149
+GDV+YN+ V+ P+G + +YRK+ F + G+ F VFD+ + + GV IC+D
Sbjct 91 DGDVLYNSAVVVGPRG-FIGKYRKIHLFYREKFFFEPGDLGFRVFDLGFM-KVGVMICFD 148
Query 150 MWFPETTRTLVAMGAEVILHPTMTDTIDRDIELSIARASAAQNQVYFIDINGVGDGG--- 206
+FPE+ RTL GA+VI HP + A +N+VY + + VG+
Sbjct 149 WFFPESARTLALKGADVIAHPA---NLVMPYAPRAMPIRALENKVYTVTADRVGEERGLK 205
Query 207 -IGRSIVIDPSGYVLHQ-SEAGPEIIPIDVDLEKVRRERRRGLRSLGQPLKSFRDR 260
IG+S++ P VL SE E+ ++DL VR +R L + F+DR
Sbjct 206 FIGKSLIASPKAEVLSMASETEEEVGVAEIDLYLVRNKRINDLNDI------FKDR 255
>Q2T9R6 Omega-amidase NIT2 [Bos taurus]
Length=276
Score = 87.0 bits (214), Expect = 2e-23
Identities = 71/263 (27%), Positives = 129/263 (49%), Gaps = 25/263 (10%)
Query 2 LSNFGIAGIQMQVSAFTS-NVEQMGNYMRHIRMRYPWVKMVLFSELAPLGPKH--STAEV 58
++ F +A IQ+QVS+ S N+ + +R + + + +P G K+ AE
Sbjct 1 MATFRLALIQLQVSSIKSENLTRACGLIREASKQGAQIVSLPECFNSPYGTKYFPDYAEK 60
Query 59 FPSQTEAQLAELARETGLWLIPGSLFERVESPEGDVIYNTLSVINPQGQIVARYRKMFPF 118
P + +L+E+A+E +++I GS+ E+ + +YNT +V P G ++ ++RK+ F
Sbjct 61 IPGDSTQKLSEVAKECSMYVIGGSIPEK----DAGKLYNTCAVFGPDGTLLVKHRKLHLF 116
Query 119 R---------PYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLVAMGAEVILH 169
++++ G+ F +FD P R G+ ICYD+ F E + G +++++
Sbjct 117 DIDVPGKITFQESETLSPGDSFSLFDTPYC-RVGLGICYDIRFAELAQIYAQRGCQLLVY 175
Query 170 PTMTD--TIDRDIELSIARASAAQNQVYFIDINGVGDG-----GIGRSIVIDPSGYVLHQ 222
P + T EL + R A NQVY + D G S V++P G VL +
Sbjct 176 PGAFNLTTGPAHWEL-LQRGRAVDNQVYVATASPARDEKASYVAWGHSTVVNPWGEVLAK 234
Query 223 SEAGPEIIPIDVDLEKVRRERRR 245
+ I+ D+DL+K+ R++
Sbjct 235 AGTEETIVYADIDLKKLAEIRQQ 257
>Q28IE5 Omega-amidase NIT2 [Xenopus tropicalis]
Length=276
Score = 85.9 bits (211), Expect = 6e-23
Identities = 68/232 (29%), Positives = 115/232 (50%), Gaps = 35/232 (15%)
Query 47 APLGPKH--STAEVFPSQTEAQLAELARETGLWLIPGSLFERVESPEGDVIYNTLSVINP 104
+P G K+ AE P ++ +L+++A+E G++LI GS+ E + +YNT +V P
Sbjct 47 SPYGTKYFPEYAEKIPGESTERLSQVAKECGIYLIGGSIPEE----DSGKLYNTCAVFGP 102
Query 105 QGQIVARYRKMFPFR---------PYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPET 155
G ++ ++RK+ F ++++ G+ F VF+ P + GV ICYD+ F E
Sbjct 103 DGTLLVKHRKIHLFDIDVPGKIRFQESETLSPGDSFSVFETPYC-KVGVGICYDIRFAEL 161
Query 156 TRTLVAMGAEVILHP---TMTDTIDRDIELSIARASAAQNQVYFIDINGVGDG-----GI 207
+ G +++++P MT T EL + RA A NQVY + D
Sbjct 162 AQLYSKKGCQLLVYPGAFNMT-TGPAHWEL-LQRARALDNQVYVATASPARDEKASYVAW 219
Query 208 GRSIVIDPSGYVLHQSEAGPEIIPIDVDLE---------KVRRERRRGLRSL 250
G S ++ P G V+ ++ + +I D+DLE +RR+RR L S+
Sbjct 220 GHSTIVSPWGEVIAKAGSEETVISADIDLEYLAEIREQIPIRRQRRHDLYSV 271
>Q32LH4 Deaminated glutathione amidase [Bos taurus]
Length=328
Score = 86.7 bits (213), Expect = 6e-23
Identities = 60/197 (30%), Positives = 93/197 (47%), Gaps = 18/197 (9%)
Query 66 QLAELARETGLWLIPGSLFERVESPEG-DVIYNTLSVINPQGQIVARYRKMF-------- 116
+ +LARE GLWL G ER + E IYN ++N G +VA YRK
Sbjct 113 EYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVIMNNMGSVVATYRKTHLCDVEIPG 172
Query 117 --PFRPYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLVAMGAEVILHPTMTD 174
P R ++ G + P G+ G++ICYDM FPE + LV GAE++ +P+
Sbjct 173 QGPMRESNSTIPGPSLESPISTP-AGKIGLAICYDMRFPELSLALVQAGAEILTYPSAFG 231
Query 175 TIDRDIELSI-ARASAAQNQVYFIDINGVG-----DGGIGRSIVIDPSGYVLHQSEAGPE 228
++ + RA A + Q Y + G G S+V+DP G V+ + GP
Sbjct 232 SVTGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPG 291
Query 229 IIPIDVDLEKVRRERRR 245
+ +DL +++ R++
Sbjct 292 LCLARIDLNYLQQLRKQ 308
>B9IW18 Formamidase [Bacillus cereus (strain Q1)]
Length=332
Score = 86.3 bits (212), Expect = 9e-23
Identities = 74/250 (30%), Positives = 117/250 (47%), Gaps = 20/250 (8%)
Query 2 LSNFGIAGIQMQVSAFTSN------VEQMGNYMRHIRMRYPWVKMVLFSELAPLG--PKH 53
+S F A IQ V S ++Q+ + + YP +++++F E + G K
Sbjct 11 ISGFLAALIQYPVPVVESRADIDKQIKQIIKTIHSTKAGYPGLELIVFPEYSTQGLNTKK 70
Query 54 STAEVF----PSQTEAQLAELARETGLWLIPGSLFERVESPEGDVIYNTLSVINPQGQIV 109
T E F P AE +E+ ++ + SL ER +P+G YNT +I+PQG+++
Sbjct 71 WTTEEFLCTVPGPETDLFAEACKESEVYGV-FSLMER--NPDGGEPYNTAIIIDPQGEMI 127
Query 110 ARYRKMFPFRPYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLVAMGAEVILH 169
+YRK+ P+ P E AG V D P + V IC+D FPE R GA V++
Sbjct 128 LKYRKLNPWVPVEPWKAGDLGLPVCDGPGGSKLAVCICHDGMFPEVAREAAYKGANVLIR 187
Query 170 PTMTDTIDRDIELSIARASAAQNQVYFIDINGVGDGGI----GRSIVIDPSGYVLHQSEA 225
+ T + + R++A QN +Y + +N G G+ G V + G L Q
Sbjct 188 ISGYSTQVSEQWMLTNRSNAWQNLMYTLSVNLAGYDGVFYYFGEGQVCNFDGTTLVQGHR 247
Query 226 GP-EIIPIDV 234
P EI+ +V
Sbjct 248 NPWEIVTAEV 257
>P49954 Omega-amidase NIT3 [Saccharomyces cerevisiae (strain ATCC
204508 / S288c)]
Length=291
Score = 84.3 bits (207), Expect = 3e-22
Identities = 75/279 (27%), Positives = 128/279 (46%), Gaps = 40/279 (14%)
Query 19 SNVEQMGNYMRHIRMRYPWVKMVLFSELAPLGPKHST------AEVF----PSQTEAQLA 68
+N+++ ++ P K+V+ E +ST +EV PS + L+
Sbjct 27 ANLQRAATFIERAMKEQPDTKLVVLPEC--FNSPYSTDQFRKYSEVINPKEPSTSVQFLS 84
Query 69 ELARETGLWLIPGSLFERVESPEGDVIYNTLSVINPQGQIVARYRKMFPFRP-------- 120
LA + + L+ G++ E P+ D IYNT + N G+++ ++RK+ F
Sbjct 85 NLANKFKIILVGGTIPEL--DPKTDKIYNTSIIFNEDGKLIDKHRKVHLFDVDIPNGISF 142
Query 121 -YEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLVAMGAEVILHPTMTDTIDRD 179
++++ G + D + G+FGV ICYDM FPE GA +++P+ +T+
Sbjct 143 HESETLSPGEKSTTIDT-KYGKFGVGICYDMRFPELAMLSARKGAFAMIYPSAFNTVTGP 201
Query 180 IELS-IARASAAQNQVYFIDINGVGD-----GGIGRSIVIDPSGYVLHQSEAGPEIIPID 233
+ +AR+ A NQVY + + + G SIV+DP G ++ ++ G EII +
Sbjct 202 LHWHLLARSRAVDNQVYVMLCSPARNLQSSYHAYGHSIVVDPRGKIVAEAGEGEEIIYAE 261
Query 234 VDLEKVRRERRRGLRSLGQPLKSFRDRDCNFTVYQDTTA 272
+D E + R+ PL R F VY D A
Sbjct 262 LDPEVIESFRQ------AVPLTKQR----RFDVYSDVNA 290
>Q8VDK1 Deaminated glutathione amidase [Mus musculus]
Length=323
Score = 84.7 bits (208), Expect = 3e-22
Identities = 61/196 (31%), Positives = 93/196 (47%), Gaps = 18/196 (9%)
Query 66 QLAELARETGLWLIPGSLFERVESPE-GDVIYNTLSVINPQGQIVARYRKMF-------- 116
Q ++LARE G+WL G ER + E IYN ++N +G +VA YRK
Sbjct 108 QYSQLARECGIWLSLGGFHERGQDWEQNQKIYNCHVLLNSKGSVVASYRKTHLCDVEIPG 167
Query 117 --PFRPYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLVAMGAEVILHPTMTD 174
P R + GG P G+ G++ICYDM FPE + L GAE++ +P+
Sbjct 168 QGPMRESNYTKPGGTLEPPVKTP-AGKVGLAICYDMRFPELSLKLAQAGAEILTYPSAFG 226
Query 175 TIDRDIELSI-ARASAAQNQVYFIDINGVG-----DGGIGRSIVIDPSGYVLHQSEAGPE 228
++ + RA A ++Q Y I G G S+V+DP G V+ + GP
Sbjct 227 SVTGPAHWEVLLRARAIESQCYVIAAAQCGRHHETRASYGHSMVVDPWGTVVARCSEGPG 286
Query 229 IIPIDVDLEKVRRERR 244
+ +DL +++ R+
Sbjct 287 LCLARIDLHFLQQMRQ 302
>Q86X76 Deaminated glutathione amidase [Homo sapiens]
Length=327
Score = 83.2 bits (204), Expect = 1e-21
Identities = 60/196 (31%), Positives = 91/196 (46%), Gaps = 18/196 (9%)
Query 66 QLAELARETGLWLIPGSLFERVESPEG-DVIYNTLSVINPQGQIVARYRKMF-------- 116
+ +LARE GLWL G ER + E IYN ++N +G +VA YRK
Sbjct 112 EYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPG 171
Query 117 --PFRPYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLVAMGAEVILHPTMTD 174
P ++ G + P G+ G+++CYDM FPE + L GAE++ +P+
Sbjct 172 QGPMCESNSTMPGPSLESPVSTP-AGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFG 230
Query 175 TIDRDIELSI-ARASAAQNQVYFIDINGVG-----DGGIGRSIVIDPSGYVLHQSEAGPE 228
+I + RA A + Q Y + G G S+V+DP G V+ + GP
Sbjct 231 SITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPG 290
Query 229 IIPIDVDLEKVRRERR 244
+ +DL +R+ RR
Sbjct 291 LCLARIDLNYLRQLRR 306
>Q8RUF8 Omega-amidase, chloroplastic [Arabidopsis thaliana]
Length=369
Score = 76.6 bits (187), Expect = 3e-19
Identities = 57/198 (29%), Positives = 92/198 (46%), Gaps = 20/198 (10%)
Query 61 SQTEAQLAELARETGLWLIPGSLFERVESPEGDVIYNTLSVINPQGQIVARYRKMFPFR- 119
S + A L+E+++ + +I GS+ ERV GD +YNT V G++ A++RK+ F
Sbjct 152 SPSTAMLSEVSKRLKITIIGGSIPERV----GDRLYNTCCVFGSDGELKAKHRKIHLFDI 207
Query 120 --------PYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLVAMGAEVILHPT 171
K++ G + D +VGR G+ ICYD+ F E A GA ++ +P
Sbjct 208 DIPGKITFMESKTLTAGETPTIVDT-DVGRIGIGICYDIRFQELAMIYAARGAHLLCYPG 266
Query 172 MTDTIDRDIELS-IARASAAQNQVYFIDINGVGDGGI-----GRSIVIDPSGYVLHQSEA 225
+ + + RA A NQ+Y + D G G S ++ P G VL +E
Sbjct 267 AFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLVGPFGEVLATTEH 326
Query 226 GPEIIPIDVDLEKVRRER 243
II ++D + + R
Sbjct 327 EEAIIIAEIDYSILEQRR 344
>O25836 Formamidase [Helicobacter pylori (strain ATCC 700392 /
26695)]
Length=334
Score = 75.9 bits (185), Expect = 4e-19
Identities = 72/299 (24%), Positives = 121/299 (40%), Gaps = 27/299 (9%)
Query 2 LSNFGIAGIQMQVSAFTS------NVEQMGNYMRHIRMRYPWVKMVLFSELAPLGPKHST 55
+ F +A IQ V S N+E + + + YP V++++F E + G +
Sbjct 11 IEGFLVAAIQFPVPIVNSRKDIDHNIESIIRTLHATKAGYPGVELIIFPEYSTQGLNTAK 70
Query 56 --AEVF----PSQTEAQLAELARETGLWLIPGSLFERVESPEGDVIYNTLSVINPQGQIV 109
+E F P + A+ +E ++ + + +S + YNT +I+PQG+I+
Sbjct 71 WLSEEFLLDVPGKETELYAKACKEAKVYGVFSIMERNPDSNKNP--YNTAIIIDPQGEII 128
Query 110 ARYRKMFPFRPYEKSVAGGNEFCVFDVPEVGRFGVSICYDMWFPETTRTLVAMGAEVILH 169
+YRK+FP+ P E G V + P + V IC+D PE R G V +
Sbjct 129 LKYRKLFPWNPIEPWYPGDLGMPVCEGPGGSKLAVCICHDGMIPELAREAAYKGCNVYIR 188
Query 170 PTMTDTIDRDIELSIARASAAQNQVYFIDINGVGDGGI----GRSIVIDPSGYVLHQSEA 225
+ T D + R++A N +Y + +N G + G + + G L Q
Sbjct 189 ISGYSTQVNDQWILTNRSNAWHNLMYTVSVNLAGYDNVFYYFGEGQICNFDGTTLVQGHR 248
Query 226 GP------EIIPIDVDLEKVRRERRRGLRSLGQ---PLKSFRDRDCNFTVYQDTTAFPY 275
P EI P D ++ + +LG K + D T +D A Y
Sbjct 249 NPWEIVTGEIYPKMADNARLSWGLENNIYNLGHRGYVAKPGGEHDAGLTYIKDLAAGKY 307
>Q9L543 Aliphatic amidase [Bacillus sp]
Length=348
Score = 75.5 bits (184), Expect = 6e-19
Identities = 62/238 (26%), Positives = 109/238 (46%), Gaps = 16/238 (7%)
Query 20 NVEQMGNYMRHIRMRYPWVKMVLFSELAPLGPKHSTAEVF------PSQTEAQLAELARE 73
N +++ + + ++ P + +V+F E + +G + E+F P + A AE ++
Sbjct 34 NAKKIADMVVGMKQGLPGMDLVVFPEYSTMGIMYDQDEMFATAASIPGEETAIFAEACKK 93
Query 74 TGLWLIPGSLFERVESPEGDVIYNTLSVINPQGQIVARYRKMFPFRPYEKSVAGGNEFCV 133
W + E+ E YNTL +IN +G+IV +YRK+ P+ P E G + V
Sbjct 94 ADTWGVFSLTGEKHEDHPNKAPYNTLVLINNKGEIVQKYRKIIPWCPIEGWYPGDTTY-V 152
Query 134 FDVPEVGRFGVSICYDMWFPETTRTLVAMGAEVILHPTMTDTIDRDIELSIARASAAQNQ 193
+ P+ + + +C D +PE R GAE+I+ ++ ++ +A+A A N
Sbjct 153 TEGPKGLKISLIVCDDGNYPEIWRDCAMKGAELIVRCQGYMYPAKEQQIMMAKAMAWANN 212
Query 194 VYFIDINGVGDGGI----GRSIVIDPSGYVLHQSEAGPE---IIPIDVDLEKVRRERR 244
Y N G G+ G S +I G L E G E I +V + ++R R+
Sbjct 213 TYVAVANATGFDGVYSYFGHSAIIGFDGRTL--GECGTEENGIQYAEVSISQIRDFRK 268
Lambda K H a alpha
0.323 0.140 0.425 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 1006020
Database: 408d203216b615d6b82f9c6ae426bbdf.SwissProt.fasta
Posted date: May 18, 2024 11:39 PM
Number of letters in database: 4,860
Number of sequences in database: 16
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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