BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 42b713072ecbfd1eac71c66b0ec407ba.SwissProt.fasta
6 sequences; 2,901 total letters
Query= ACIAD0248
Length=432
Score E
Sequences producing significant alignments: (Bits) Value
P24582 Type II methyltransferase M.NlaIII [Neisseria lactamica] 125 5e-36
P14871 Type II methyltransferase M.FokI [Planomicrobium okeanokoi... 122 2e-33
P35516 Modification methylase LlaI [Lactococcus lactis subsp. lac... 107 1e-28
P29347 Modification methylase StsI [Streptococcus sanguinis] 107 2e-28
P31118 Type II methyltransferase M.CviAII [Paramecium bursaria Ch... 100 3e-27
P14751 Type II methyltransferase M.RsrI [Cereibacter sphaeroides] 36.6 5e-06
>P24582 Type II methyltransferase M.NlaIII [Neisseria lactamica]
Length=334
Score = 125 bits (313), Expect = 5e-36
Identities = 103/350 (29%), Positives = 165/350 (47%), Gaps = 41/350 (12%)
Query 29 YLGNKRRLLH-LILEALEITGTLNSKKKNPPIFADFFAGSGVVSRLARQNGYRVIANDWE 87
Y+G+K +L + L E + G S K +F D FAG+G+V R + N +VIAND E
Sbjct 3 YIGSKLKLSNWLETEISNVAGHSLSDK----VFCDLFAGTGIVGRKFKTNVKQVIANDME 58
Query 88 PYSHALNHAILACVDDAPAFKELGGYQKAIDYLNRLPEVKGWVTHNLCPRNDDIYDPSRD 147
YS+ LN + EL + L +LP +G + + C +
Sbjct 59 YYSYVLNRNYIGNCQSILKAGEL------LQRLEQLPPREGLIYQHYC------LGSGSE 106
Query 148 RLFFKRRNGMRIDAIRQQIATWQAQGAINDVEMSALLAPLLYSASFVSNTSGVFKSFHQG 207
R +F NG +IDA+R QI W+ I++ LLA LL A V+NT+ V+ +F +
Sbjct 107 RQYFSDENGKKIDAVRIQIEEWKNTRYIDEDTYYFLLATLLEGADKVANTASVYGAFLKN 166
Query 208 WGGRTQTALERIESLLWLTPSRFCDVGDSKRPAAEMWCVDAQHLATQMSSFEVDVAYLDP 267
++AL+ + L P+ F ++G +++ DA L +S D+ YLDP
Sbjct 167 L---KKSALKPLS----LEPALF-EIGSDGH---QVYQADANQLIKNISG---DILYLDP 212
Query 268 PYNQHAYSSNYHVLNALTLWDQVDLPSPDTKGYKSGIDRAWRKERPSPYNSSKYAKDAYE 327
PYN Y +NYH+LN++ L+D T K+G+ R+ S Y S +E
Sbjct 213 PYNARQYGANYHLLNSIALYDDF------TPKGKTGL----REYSRSKYCSKSDVVPVFE 262
Query 328 KLLETINARYILTSYSTDGNIEPKDLLTANLKRGKVTLLTQDVPRYRVSK 377
L+ + +YI SY+ +G + + + GK L+ + R++ K
Sbjct 263 ALIRDADFQYIFLSYNNEGLMSVGQVREIFERFGKYDLVQTEYRRFKADK 312
Score = 18.5 bits (36), Expect = 2.7
Identities = 8/19 (42%), Positives = 10/19 (53%), Gaps = 0/19 (0%)
Query 4 EASVYHKRRHAARTTDEYL 22
+A R H A +T EYL
Sbjct 309 KADKTENRNHKANSTFEYL 327
>P14871 Type II methyltransferase M.FokI [Planomicrobium okeanokoites]
Length=647
Score = 122 bits (305), Expect = 2e-33
Identities = 106/368 (29%), Positives = 183/368 (50%), Gaps = 41/368 (11%)
Query 29 YLGNKRRLLHLILEALEITGTLNSKKKNPPIFADFFAGSGVVSRLARQNGYRVIANDWEP 88
++G+K LL I E +E + K + +F D F+G+G+V +++ Y+V++ND
Sbjct 3 FIGSKVNLLDNIQEVIE-----ENVKDDAHVFMDLFSGTGIVGENFKKD-YQVLSNDSLY 56
Query 89 YSHALNHAILACVDDAPAFKELG--GYQKAIDYL-NRLPEVKG--WVTHNLCPRNDDIYD 143
+S+ L A + + P F EL G ++ + YL N E+ ++THN P
Sbjct 57 FSYILLKAKIEN-NSIPNFSELKKIGIKEPLHYLENEEFEISHEFFLTHNYSPYM----- 110
Query 144 PSRDRLFFKRRNGMRIDAIRQQIATWQAQGAINDVEMSALLAPLLYSASFVSNTSGVFKS 203
+R++F N RID IR + W+ + IN++E + LLA L+ + F+SN SG + +
Sbjct 111 -GCERMYFTVENASRIDFIRLTLNRWKNESLINELEFAYLLAILIEAVPFISNISGTYGA 169
Query 204 FHQGWGGRTQTALERIESLLWLTPSRFCDVGDSKRPAAEMWCVDAQHLATQMSSFEVDVA 263
+ + W R L+ R D+G++ A + + DA L ++ D+
Sbjct 170 YLKHWDKRALGKLK----------LRTLDIGNN-HYANKTYNEDANSLIEKVYG---DIL 215
Query 264 YLDPPYNQHAYSSNYHVLNALTLWDQVDLPSPDTKGYKSGIDRAWRKERPSPYNSSKYAK 323
Y+DPPYN Y SNYH+L + L+D P+ G K+G+ R + E S Y K
Sbjct 216 YIDPPYNGRQYISNYHLLETIALYDY-----PEIYG-KTGL-RPY-VESKSLYCQKKEVG 267
Query 324 DAYEKLLETINARYILTSYSTDGNIEPKDLLTANLKRG-KVTLLTQDVPRYRVSKQRQSE 382
+A+ L+E N R+IL SYS++G + +++ + G T +P + + + E
Sbjct 268 NAFNHLIEKANFRHILVSYSSEGLLLEEEIESILKSHGLPETYRIYKMPYRKYKSKHKQE 327
Query 383 RARVLEFI 390
+ + E+I
Sbjct 328 ASELHEYI 335
Score = 20.0 bits (40), Expect = 0.97
Identities = 6/9 (67%), Positives = 7/9 (78%), Gaps = 0/9 (0%)
Query 261 DVAYLDPPY 269
D+ Y DPPY
Sbjct 543 DLVYCDPPY 551
Score = 17.3 bits (33), Expect = 6.6
Identities = 5/9 (56%), Positives = 8/9 (89%), Gaps = 0/9 (0%)
Query 271 QHAYSSNYH 279
QH+YS++ H
Sbjct 619 QHSYSNSSH 627
>P35516 Modification methylase LlaI [Lactococcus lactis subsp.
lactis]
Length=622
Score = 107 bits (267), Expect = 1e-28
Identities = 104/373 (28%), Positives = 170/373 (46%), Gaps = 46/373 (12%)
Query 29 YLGNKRRLLHLILEALEITGTLNSKKKNPPIFADFFAGSGVVSRLARQNGYRVIANDWEP 88
YLGNK LL+ I + ++ FAD FAG+G V + Y V++ND+
Sbjct 3 YLGNKTNLLNFIQQVIK------KHDIQGQTFADLFAGTGSVGDYFKGE-YTVLSNDYMY 55
Query 89 YSHALNHAILACVDDAPAFKELGGYQKA-IDYLNRLPEVK--GWVTHNLCPRNDDIYDPS 145
+S ++ A L + + Y K +LN G+ +N Y P
Sbjct 56 FSKVISEAKLLNSEKPKFDSFVKRYGKTPFQWLNEREYTPNDGYFVYNN-------YTPR 108
Query 146 RDRLFFKRRNGMRIDAIRQQIATWQAQGAINDVEMSALLAPLLYSASFVSNTSGVFKSFH 205
+R++ N ++ID +R I +G I+ E S LLA LL S + VSNTSG +++F
Sbjct 109 AERMYLTEENALKIDGMRLDIEELFQEGVISKAEYSYLLASLLESVTKVSNTSGTYQAFF 168
Query 206 QGWGGRTQTALERIESLLWLTPSRFCDVGDSKRPAAEMWCV--DAQHLATQMSSFEVDVA 263
+ W R AL++ + ++ DS + + C + L ++S D+A
Sbjct 169 KFWESR---ALKKFTIMP-------LEMKDSLSVSKDNRCFNKNTNRLVREISG---DIA 215
Query 264 YLDPPYNQHAYSSNYHVLNALTLWDQVDLPSPDTKGYKSGIDRAWRKERPSPYNSSKYAK 323
Y+DPPY Y+++YHVL + +D +P+ G K+G R ++E S Y++ A
Sbjct 216 YIDPPYTITQYTNSYHVLETIARYD-----NPELFG-KTG--RRVKREF-SGYSNKSKAY 266
Query 324 DAYEKLLETINARYILTSYSTDGNIEPKDLLTANLKR----GKVTLLTQDVPRYRVSKQR 379
+E L IN ++L SYS +I P D L +R G V + T + Y +
Sbjct 267 YEFEDLFRQINFTHVLVSYSNQ-SIVPLDELVDLARRFAVDGIVEVETNEYREYSTNNSS 325
Query 380 QSERARVLEFIVI 392
+ L+ ++I
Sbjct 326 MKGEGKKLQEVII 338
Score = 18.9 bits (37), Expect = 2.2
Identities = 11/35 (31%), Positives = 17/35 (49%), Gaps = 3/35 (9%)
Query 236 SKRPAAEMWCVDAQHLATQMSSFEVD-VAYLDPPY 269
++ P EM + A + ++ D V Y DPPY
Sbjct 499 TRAPEVEMRL--GSYSAINFNEYDDDTVFYFDPPY 531
>P29347 Modification methylase StsI [Streptococcus sanguinis]
Length=653
Score = 107 bits (266), Expect = 2e-28
Identities = 98/366 (27%), Positives = 165/366 (45%), Gaps = 39/366 (11%)
Query 29 YLGNKRRLLHLILEALEITGTLNSKKKNPPIFADFFAGSGVVSRLARQNGYRVIANDWEP 88
Y+G+K+ LL I + ++ + +F D FAG+ VV+ +Q Y V +ND
Sbjct 3 YIGSKKLLLPEIKKMVD-----KHTDGSEEVFLDLFAGTNVVANYFKQF-YTVYSNDMLF 56
Query 89 YSHALNHAILACVDDAPAFKEL--GGYQKAIDYLNRLPEVKGWVTHNLCPRNDDIYDPSR 146
+S+ A + + P+F +L G + YL L V + Y P+
Sbjct 57 FSYVNAKATIEN-NSKPSFSKLIQAGISSPMTYLQNLE-----VNDETIGYYEVAYSPTG 110
Query 147 DRLFFKRRNGMRIDAIRQQIATWQAQGAINDVEMSALLAPLLYSASFVSNTSGVFKSFHQ 206
+ + N ++D IR QI +W+ Q + + E LL+ L+ + F+SNT+G + +F +
Sbjct 111 EANYLSVHNAKKLDIIRSQIESWKNQNLLTEHEYYYLLSSLIEALPFISNTTGTYGAFLK 170
Query 207 GWGGRTQTALERIESLLWLTPSRFCDVGDSKRPAAEMWCVDAQHLATQMSSFEVDVAYLD 266
W R+ LE + F +SK+ A + DA L + + D+ Y+D
Sbjct 171 HWDKRSLNDLELQD---------FTIFDNSKQNKA--FNEDANEL---VQKIKADIVYID 216
Query 267 PPYNQHAYSSNYHVLNALTLWDQVDLPSPDTKGYKSGIDRAWRKERPSPYNSSKYAKDAY 326
PYN Y+SNYH+L + + P KG D W K S Y + A A
Sbjct 217 TPYNSRQYASNYHLLENVARNEH-----PTLKGITKIFD--W-KNLKSDYATKGKALVAM 268
Query 327 EKLLETINARYILTSYSTDGNIEPKDLLTANLKRGKVTLLT--QDVPRYRVSKQRQSERA 384
L++ IN+ +I+ SY+ +G I +D LT LK V + + +P + + S+
Sbjct 269 RDLIQNINSTHIILSYNNEGIISEED-LTNILKEFSVDGIVDIKKIPYRKYQSKNVSKNK 327
Query 385 RVLEFI 390
+ E +
Sbjct 328 EIYELL 333
Score = 21.6 bits (44), Expect = 0.32
Identities = 18/57 (32%), Positives = 23/57 (40%), Gaps = 11/57 (19%)
Query 29 YLGNKRRLLHLILEALEITGTLNSKKKNPPIFADFFAGSGVVSRLARQNGYRVIAND 85
Y+G K +LL+ IL KN F D F+G V + Y I ND
Sbjct 369 YIGGKYKLLNQILPLFP---------KNINTFVDIFSGGANVGINVKAKKY--IFND 414
Score = 20.8 bits (42), Expect = 0.56
Identities = 7/9 (78%), Positives = 7/9 (78%), Gaps = 0/9 (0%)
Query 261 DVAYLDPPY 269
D YLDPPY
Sbjct 541 DFIYLDPPY 549
Score = 19.2 bits (38), Expect = 1.7
Identities = 8/20 (40%), Positives = 13/20 (65%), Gaps = 0/20 (0%)
Query 257 SFEVDVAYLDPPYNQHAYSS 276
S ++V YLD YN +++S
Sbjct 610 SRNLNVNYLDHNYNNSSHNS 629
>P31118 Type II methyltransferase M.CviAII [Paramecium bursaria
Chlorella virus 1]
Length=326
Score = 100 bits (249), Expect = 3e-27
Identities = 91/351 (26%), Positives = 159/351 (45%), Gaps = 52/351 (15%)
Query 27 VPYLGNKRRLLHLILEALEITGTLNSKKKNPPI--FADFFAGSGVVSRLARQNGYRVIAN 84
+ Y+G+K +L I E + SK+ + FAD FAGS +++ A + Y I+N
Sbjct 4 IGYIGSKLKLKDWIFEEI-------SKRTDDTYTKFADLFAGSCIMTHEALEKKYECISN 56
Query 85 DWEPYSHALNHAILACVDDAPAFKELGGYQKAIDYLNRLPEV-KGWVTHNLCPRNDDIYD 143
D E YS+ + + + P +L + +D L+ V G+VT Y
Sbjct 57 DLETYSYVIMNGL-----KCPFSDKLQNIIETLDDLDTKDMVIPGFVTLT--------YS 103
Query 144 PSRDRLFFKRRNGMRIDAIRQQIATWQAQGAINDVEMSALLAPLLYSASFVSNTSGVFKS 203
P +R++F MRID IR+ I + + ++ E + LLA LL SA V NTS V+ +
Sbjct 104 PRGNRMYFTEDIAMRIDIIRENIERMKER--VSTDEYNFLLASLLTSADSVKNTSVVYGA 161
Query 204 FHQGWGGRTQTALERIESLLWLTPSRFCDVGDSKRPAAEMWCVDAQHLATQMSSFEVDVA 263
+ + + +TAL+R+ T S + A E+ + D+A
Sbjct 162 YLKKF---KKTALKRMVFAPLHTRSTTVTLETFNEDATEL-------------EIKTDIA 205
Query 264 YLDPPYNQHAYSSNYHVLNALTLWDQVDLPSPDTKGYKSGIDRAWRKERPSPYNSSKYAK 323
Y+DPPYN Y +NY VLN + ++ YK S + +
Sbjct 206 YVDPPYNSRQYGANYFVLNQILTPKEIGNGVTGLPEYKK-----------SSFCRKQEVA 254
Query 324 DAYEKLLETINARYILTSYSTDGNIEPKDLLTANLKRGKVTLLTQDVPRYR 374
++ K+L+ ++AR + SYS++ + D++ + GK ++ ++ R++
Sbjct 255 MSFHKMLKNVSARLFVISYSSESLLSKGDMVALLSQYGKCEVVVRNHKRFK 305
>P14751 Type II methyltransferase M.RsrI [Cereibacter sphaeroides]
Length=319
Score = 36.6 bits (83), Expect = 5e-06
Identities = 24/59 (41%), Positives = 28/59 (47%), Gaps = 19/59 (32%)
Query 62 DFFAGSGVVSRLARQNGYRVIANDWEPYSHALNHAILACVDDAPAFKELGGYQKAIDYL 120
DFFAGSGV +R+A Q G I C D AP FKE YQK + +L
Sbjct 248 DFFAGSGVTARVAIQEGRNSI-----------------CTDAAPVFKEY--YQKQLTFL 287
Score = 18.1 bits (35), Expect = 3.6
Identities = 6/11 (55%), Positives = 7/11 (64%), Gaps = 0/11 (0%)
Query 260 VDVAYLDPPYN 270
V + DPPYN
Sbjct 59 VQLIICDPPYN 69
Lambda K H a alpha
0.320 0.135 0.412 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 1018197
Database: 42b713072ecbfd1eac71c66b0ec407ba.SwissProt.fasta
Posted date: May 8, 2024 2:56 PM
Number of letters in database: 2,901
Number of sequences in database: 6
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
