BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 76a5550a11b20f743d06a2bc6d2dbc28.SwissProt.fasta
11 sequences; 3,147 total letters
Query= ACIAD0420
Length=213
Score E
Sequences producing significant alignments: (Bits) Value
Q5SHW1 tRNA (guanine-N(7)-)-methyltransferase [Thermus thermophil... 51.6 2e-11
Q2YCZ3 tRNA (guanine-N(7)-)-methyltransferase [Nitrosospira multi... 48.5 1e-10
O25443 tRNA (guanine-N(7)-)-methyltransferase [Helicobacter pylor... 48.5 2e-10
Q9CCZ9 tRNA (guanine-N(7)-)-methyltransferase [Mycobacterium lepr... 44.3 4e-09
B8ZTI4 tRNA (guanine-N(7)-)-methyltransferase [Mycobacterium lepr... 44.3 4e-09
Q0K7S5 tRNA (guanine-N(7)-)-methyltransferase [Cupriavidus necato... 44.3 4e-09
Q17Y01 tRNA (guanine-N(7)-)-methyltransferase [Helicobacter acino... 44.3 5e-09
Q2KYW5 tRNA (guanine-N(7)-)-methyltransferase [Bordetella avium (... 43.1 1e-08
Q5WE98 tRNA (guanine-N(7)-)-methyltransferase [Shouchella clausii... 42.7 1e-08
Q0AJ68 tRNA (guanine-N(7)-)-methyltransferase [Nitrosomonas eutro... 42.4 2e-08
Q9ZL96 tRNA (guanine-N(7)-)-methyltransferase [Helicobacter pylor... 42.7 2e-08
>Q5SHW1 tRNA (guanine-N(7)-)-methyltransferase [Thermus thermophilus
(strain ATCC 27634 / DSM 579 / HB8)]
Length=311
Score = 51.6 bits (122), Expect = 2e-11
Identities = 38/158 (24%), Positives = 69/158 (44%), Gaps = 2/158 (1%)
Query 26 PICVEIGAGKGKHALLFSGLQPAQTLYAVERTKEKFLAMQKQQVLDPRPNLYPVHADALP 85
P+ +EIG G G+ + +P + E + L ++ + N+ H
Sbjct 24 PLVLEIGFGDGRFTAELARSRPDWLVLGAEVSAASVLRALRRMRREGLANVRLYHGQGPF 83
Query 86 WIVHTLYPVQVEQFFILYPNPEPHNPAQ--RWLNMPFFEFLLSRLKPEGTITLASNIPEY 143
+ + + P ++Q + +P+P P Q R L FF L +RLKP G++ L ++ EY
Sbjct 84 ALRNLVLPGTLDQVIVNFPDPWPKKRHQERRLLREAFFRRLSTRLKPGGSLLLTTDHEEY 143
Query 144 IAEAAQQLEQLWQLPFEKQVIASGSARTHFEIKYLERG 181
A ++ E+ E + RT + +K+ E G
Sbjct 144 FRFALEEAERTGLYRVEVRPPPEAHLRTKYALKWKEAG 181
Score = 15.8 bits (29), Expect = 9.4
Identities = 10/33 (30%), Positives = 20/33 (61%), Gaps = 5/33 (15%)
Query 4 PIRQFQ-AHRM---QAPRHFKAIENTPICVEIG 32
P+R++ AH + + P+ A+E TP+ ++ G
Sbjct 201 PLRRYDVAHALLSGELPQDL-ALEKTPVRLKEG 232
>Q2YCZ3 tRNA (guanine-N(7)-)-methyltransferase [Nitrosospira multiformis
(strain ATCC 25196 / NCIMB 11849 / C 71)]
Length=229
Score = 48.5 bits (114), Expect = 1e-10
Identities = 43/162 (27%), Positives = 70/162 (43%), Gaps = 7/162 (4%)
Query 24 NTPICVEIGAGKGKHALLFSGLQPAQTLYAVERTKEKFLAMQKQQVLDPRPNLYPVHADA 83
+ P +EIG G G+ L + P A E ++ KQ N+ + DA
Sbjct 53 SAPKFLEIGFGMGETTALIAQAHPQNDYLAAEVHTPGMGSLLKQVEELELTNIRVIQHDA 112
Query 84 LPWIVHTLYPVQVEQFFILYPNPEP--HNPAQRWLNMPFFEFLLSRLKPEGTITLASNIP 141
+ + H L P ++ + +P+P P + +R + F + L SRLKP G I +A++
Sbjct 113 VDVLQHALPPECLDGVHVFFPDPWPKARHHKRRLIQAEFVDLLCSRLKPGGYIHVATDWE 172
Query 142 EYIAEAAQQLEQLWQLPFEKQVIASGSARTHFE--IKYLERG 181
+Y A Q LE L P + R + K+ +RG
Sbjct 173 DY---AEQILEVLSGEPHLSNTAVGYAPRPEYRPLTKFEQRG 211
>O25443 tRNA (guanine-N(7)-)-methyltransferase [Helicobacter pylori
(strain ATCC 700392 / 26695)]
Length=393
Score = 48.5 bits (114), Expect = 2e-10
Identities = 42/177 (24%), Positives = 82/177 (46%), Gaps = 3/177 (2%)
Query 26 PICVEIGAGKGKHALLFSGLQPAQTLYAVE-RTKEKFLAMQKQQVLDPRPNLYPVHADAL 84
PI +EIG G G+H + + P +T +E T A+++ ++LD + NL+ + D
Sbjct 120 PILIEIGFGSGRHLIELAKNNPTKTCLGIEIHTPSIAQALKQIELLDLK-NLHILQGDG- 177
Query 85 PWIVHTLYPVQVEQFFILYPNPEPHNPAQRWLNMPFFEFLLSRLKPEGTITLASNIPEYI 144
++ ++ + E+ F+ +P P +R L+ F L LKP G + L ++ Y
Sbjct 178 RLVLESMPNHRCEKIFVHFPVPWNEKKHRRVLSEKFLNEALRVLKPRGFLELRTDDSLYF 237
Query 145 AEAAQQLEQLWQLPFEKQVIASGSARTHFEIKYLERGEPCQQLVISKPATYQTRFDN 201
++ + + +Q E + A + +E ++ + + L I +T FDN
Sbjct 238 EDSLKLALKNFQCEIEIKKNAQIPVVSKYEARWNKLKKDIYDLRIYSLEWNETPFDN 294
>Q9CCZ9 tRNA (guanine-N(7)-)-methyltransferase [Mycobacterium
leprae (strain TN)]
Length=230
Score = 44.3 bits (103), Expect = 4e-09
Identities = 30/128 (23%), Positives = 59/128 (46%), Gaps = 2/128 (2%)
Query 24 NTPICVEIGAGKGKHALLFSGLQPAQTLYAVERTKEKFLAMQKQQVLDPRPNLYPVHADA 83
+T + +E+G G G L + +P + AVE + + D N+ +H +A
Sbjct 55 STLVVLEVGCGSGTATLAMAQHEPDIDVIAVEVYRRGLAQLLCAIDRDQVHNIRMIHGNA 114
Query 84 LPWIVHTLYPVQVEQFFILYPNPEP--HNPAQRWLNMPFFEFLLSRLKPEGTITLASNIP 141
L + + + P + + +P+P P + +R+L E + RL P G + A++ P
Sbjct 115 LYVLQYLIAPRSLTGVRVFFPDPWPKVRHHKRRFLQPATVELIADRLLPGGVLHTATDHP 174
Query 142 EYIAEAAQ 149
+Y + A+
Sbjct 175 DYAKQIAK 182
>B8ZTI4 tRNA (guanine-N(7)-)-methyltransferase [Mycobacterium
leprae (strain Br4923)]
Length=230
Score = 44.3 bits (103), Expect = 4e-09
Identities = 30/128 (23%), Positives = 59/128 (46%), Gaps = 2/128 (2%)
Query 24 NTPICVEIGAGKGKHALLFSGLQPAQTLYAVERTKEKFLAMQKQQVLDPRPNLYPVHADA 83
+T + +E+G G G L + +P + AVE + + D N+ +H +A
Sbjct 55 STLVVLEVGCGSGTATLAMAQHEPDIDVIAVEVYRRGLAQLLCAIDRDQVHNIRMIHGNA 114
Query 84 LPWIVHTLYPVQVEQFFILYPNPEP--HNPAQRWLNMPFFEFLLSRLKPEGTITLASNIP 141
L + + + P + + +P+P P + +R+L E + RL P G + A++ P
Sbjct 115 LYVLQYLIAPRSLTGVRVFFPDPWPKVRHHKRRFLQPATVELIADRLLPGGVLHTATDHP 174
Query 142 EYIAEAAQ 149
+Y + A+
Sbjct 175 DYAKQIAK 182
>Q0K7S5 tRNA (guanine-N(7)-)-methyltransferase [Cupriavidus necator
(strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442
/ H16 / Stanier 337)]
Length=263
Score = 44.3 bits (103), Expect = 4e-09
Identities = 42/134 (31%), Positives = 64/134 (48%), Gaps = 11/134 (8%)
Query 26 PICVEIGAGKGKHALLFSGLQPAQTLYAVERTKEKFLAMQKQQVLDPR--PNLYPVHADA 83
P +EIG G G+ +GL+P E + A+ K ++D R N+ + DA
Sbjct 87 PSILEIGFGMGETTAHIAGLRPQDNFLGCEVHEPGVGALLK--LIDERGLANVRILQHDA 144
Query 84 LPWIVHTLYPVQVEQFFILYPNP---EPHNPAQRWLNMPFFEFLLSRLKPEGTITLASNI 140
+ I H L ++ I +P+P + HN +R + P + L +RLKP G I A++
Sbjct 145 VEVIAHMLTDDCLDGVHIYFPDPWHKKRHNK-RRLVQPPLVKVLAARLKPGGYIHCATDW 203
Query 141 PEYIAEAAQQLEQL 154
EY A Q LE L
Sbjct 204 EEY---AHQMLEVL 214
>Q17Y01 tRNA (guanine-N(7)-)-methyltransferase [Helicobacter acinonychis
(strain Sheeba)]
Length=398
Score = 44.3 bits (103), Expect = 5e-09
Identities = 38/176 (22%), Positives = 78/176 (44%), Gaps = 1/176 (1%)
Query 26 PICVEIGAGKGKHALLFSGLQPAQTLYAVERTKEKFLAMQKQQVLDPRPNLYPVHADALP 85
PI +EIG G G+H + + P +T +E + KQ L NL+ + D
Sbjct 120 PILMEIGFGSGRHLMELAKNNPTKTCLGIEIYTPSIAQVLKQIELLDLKNLHVLQGDG-R 178
Query 86 WIVHTLYPVQVEQFFILYPNPEPHNPAQRWLNMPFFEFLLSRLKPEGTITLASNIPEYIA 145
++ ++ + E+ F+ +P P +R L+ F + L L+P G + L ++ Y
Sbjct 179 LVLESIPHHKCEKIFVHFPVPWNDKKHRRVLSERFLDEALRVLEPNGFLELRTDDTLYFE 238
Query 146 EAAQQLEQLWQLPFEKQVIASGSARTHFEIKYLERGEPCQQLVISKPATYQTRFDN 201
++ + + ++ E + A + +E ++ + + L I + +T F+N
Sbjct 239 DSLKLALKHFKSEIEIKKNAQIPVVSKYEARWNKLKKDIYDLRIYSLESNETPFNN 294
>Q2KYW5 tRNA (guanine-N(7)-)-methyltransferase [Bordetella avium
(strain 197N)]
Length=256
Score = 43.1 bits (100), Expect = 1e-08
Identities = 41/173 (24%), Positives = 70/173 (40%), Gaps = 5/173 (3%)
Query 26 PICVEIGAGKGKHALLFSGLQPAQTLYAVERTKEKFLAMQKQQVLDPRPNLYPVHADALP 85
P +EIG G G+ + +P VE +M + PN+ + DA+
Sbjct 84 PTILEIGFGMGETTEKIALARPEDNFLGVEVFNAGVGSMLHRIETSQIPNVRIIQHDAVE 143
Query 86 WIVHTLYPVQVEQFFILYPNPEP--HNPAQRWLNMPFFEFLLSRLKPEGTITLASNIPEY 143
+ + P + + +P+P P + +R L F + L SR+KP G + A++ +Y
Sbjct 144 VVRDMIAPDSLAGVHVYFPDPWPKKRHHKRRLLQPAFVQLLASRIKPGGYLHCATDWQDY 203
Query 144 IAEAAQQLEQLWQLPFEKQVIASGSA---RTHFEIKYLERGEPCQQLVISKPA 193
+ + L L Q A A +T FE + L G L+ + A
Sbjct 204 AVQMLEVLGGEALLENTAQDYAERPAYRPQTKFETRGLRLGHGVWDLIFKRKA 256
Score = 20.0 bits (40), Expect = 0.40
Identities = 15/63 (24%), Positives = 30/63 (48%), Gaps = 12/63 (19%)
Query 146 EAAQQLEQLWQLPFEKQVIASGS-----ARTHFEIKYLERGEPCQQLVISKPATYQTRFD 200
+A +QL W +P+ + + + A T EI + GE +++ +++P D
Sbjct 55 DALEQLMGKWAIPYAPRPLNPATAFGREAPTILEIGF-GMGETTEKIALARPE------D 107
Query 201 NFM 203
NF+
Sbjct 108 NFL 110
>Q5WE98 tRNA (guanine-N(7)-)-methyltransferase [Shouchella clausii
(strain KSM-K16)]
Length=213
Score = 42.7 bits (99), Expect = 1e-08
Identities = 30/111 (27%), Positives = 51/111 (46%), Gaps = 3/111 (3%)
Query 26 PICVEIGAGKGKHALLFSGLQPAQTLYAVERTKEKFLAMQKQQVLDPRPNLYPVHADALP 85
P+ VE+G GKGK + P+ VER + L ++ + PN+ +H D +
Sbjct 40 PLYVEVGTGKGKFLTGMAKQFPSINFIGVERYESVLLTAMERVEAEKLPNVKLLHKD-VA 98
Query 86 WIVHTLYPVQVEQFFILYPNPEP--HNPAQRWLNMPFFEFLLSRLKPEGTI 134
++ +V++FFI + +P P + +R N F LK +G I
Sbjct 99 QLLEVFAENEVDRFFINFTDPWPKKKHAKRRLTNERFLTIYKQLLKQDGEI 149
>Q0AJ68 tRNA (guanine-N(7)-)-methyltransferase [Nitrosomonas eutropha
(strain DSM 101675 / C91 / Nm57)]
Length=224
Score = 42.4 bits (98), Expect = 2e-08
Identities = 40/173 (23%), Positives = 73/173 (42%), Gaps = 5/173 (3%)
Query 26 PICVEIGAGKGKHALLFSGLQPAQTLYAVERTKEKFLAMQKQQVLDPRPNLYPVHADALP 85
P +EIG+G G+ + + P + A+E ++ + NL + DA
Sbjct 52 PKILEIGSGMGETTVEIAAQHPEKDFVAIEVHAPGIGSLLDRIEKHGLTNLRIIPHDAKL 111
Query 86 WIVHTLYPVQVEQFFILYPNPEP--HNPAQRWLNMPFFEFLLSRLKPEGTITLASNIPEY 143
+ H L ++ I +P+P P + +R + F L +RLKP G + +A++ +Y
Sbjct 112 VLQHMLTGESLDGIHIFFPDPWPKARHHKRRLIQSDFVSLLCNRLKPGGYLHIATDWEDY 171
Query 144 IAEAAQQLEQLWQLPFEKQVIASGSA---RTHFEIKYLERGEPCQQLVISKPA 193
+ L +L A A T FE + ++ G + L+ +K A
Sbjct 172 AIHSLHVLSSEQRLINTAADYAPRPAYRPLTKFEQRGMKLGHTIRDLIFTKAA 224
>Q9ZL96 tRNA (guanine-N(7)-)-methyltransferase [Helicobacter pylori
(strain J99 / ATCC 700824)]
Length=400
Score = 42.7 bits (99), Expect = 2e-08
Identities = 31/115 (27%), Positives = 57/115 (50%), Gaps = 3/115 (3%)
Query 26 PICVEIGAGKGKHALLFSGLQPAQTLYAVE-RTKEKFLAMQKQQVLDPRPNLYPVHADAL 84
PI +EIG G G+H + + P T +E T A+++ ++LD + NL+ + D
Sbjct 120 PILIEIGFGSGRHLIELAKNNPTTTCLGIEIHTPSIAQALKQIELLDLK-NLHILQGDG- 177
Query 85 PWIVHTLYPVQVEQFFILYPNPEPHNPAQRWLNMPFFEFLLSRLKPEGTITLASN 139
++ ++ + E+ F+ +P P +R L+ F L LKP G + L ++
Sbjct 178 RLVLESMPNHRCEKIFVHFPVPWNEKKHRRVLSEKFLNEALRVLKPRGFLELRTD 232
Lambda K H a alpha
0.325 0.139 0.433 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 468311
Database: 76a5550a11b20f743d06a2bc6d2dbc28.SwissProt.fasta
Posted date: May 9, 2024 3:58 PM
Number of letters in database: 3,147
Number of sequences in database: 11
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
