ACIAD0602 / SwissProt Alignments

BLASTP 2.12.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.


Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.



Database: 50f93a689cb497df52363f44c1996950.SwissProt.fasta
           12 sequences; 4,696 total letters



Query= ACIAD0602

Length=347
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

Q81ST7 N-acetyl-alpha-D-glucosaminyl L-malate synthase [Bacillus ...  90.5    1e-23
Q59002 Uncharacterized glycosyltransferase MJ1607 [Methanocaldoco...  76.3    9e-19
Q9R9N1 Lipopolysaccharide core biosynthesis glycosyltransferase L...  74.3    3e-18
P42982 N-acetyl-alpha-D-glucosaminyl L-malate synthase [Bacillus ...  72.8    1e-17
Q04975 Vi polysaccharide biosynthesis protein VipC/TviE [Salmonel...  59.7    3e-13
Q0P9C5 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undeca...  58.5    6e-13
Q58459 Uncharacterized glycosyltransferase MJ1059 [Methanocaldoco...  56.2    4e-12
Q0P9C7 N-acetylgalactosamine-N,N'-diacetylbacillosaminyl-diphosph...  55.5    6e-12
P71053 Putative glycosyltransferase EpsD [Bacillus subtilis (stra...  55.5    6e-12
Q0P9C9 N,N'-diacetylbacillosaminyl-diphospho-undecaprenol alpha-1...  49.3    6e-10
P27127 Lipopolysaccharide 1,6-galactosyltransferase [Escherichia ...  48.5    1e-09
P71055 Putative glycosyltransferase EpsF [Bacillus subtilis (stra...  48.1    1e-09


>Q81ST7 N-acetyl-alpha-D-glucosaminyl L-malate synthase [Bacillus 
anthracis]
Length=381

 Score = 90.5 bits (223),  Expect = 1e-23
 Identities = 77/302 (25%), Positives = 147/302 (49%), Gaps = 40/302 (13%)

Query  34   ELHVIAH--PCYAEKFNSNVKFHAVD------FARSRWNIFLLWQLKSLIQQIQPDIVHA  85
            E+H I    P    K   N+ FH V       F    +++ L  ++  + Q+   DI+H 
Sbjct  33   EIHFITSGLPFRLNKVYPNIYFHEVTVNQYSVFQYPPYDLALASKMAEVAQRENLDILHV  92

Query  86   QAGKAAELISRIRPFL--PDIKVVTTIHGTK----------KNKSTYLV--GDAVIAVSQ  131
                   + + +   +    IK+VTT+HGT            N   + +   D V AVS 
Sbjct  93   HYAIPHAICAYLAKQMIGERIKIVTTLHGTDITVLGSDPSLNNLIRFGIEQSDVVTAVSH  152

Query  132  ALAQGI-----PNNKAHVIYNGVYPQPDLSIEHKQHLLQTIQQDFPQLDSNKKLVMCIGR  186
            +L         PN     +YN +    D  +  K+ + Q +++++  +  ++K+++ I  
Sbjct  153  SLINETHELVKPNKDIQTVYNFI----DERVYFKRDMTQ-LKKEYG-ISESEKILIHISN  206

Query  187  LEPVKNISLLIES----MQQIDANLWIIGDGSLRASLEKQVSELNIQNRVAFLGFRIDAR  242
               VK +  ++++    + ++DA L ++GDG    ++ + V  L+I++RV FLG + +  
Sbjct  207  FRKVKRVQDVVQAFAKIVTEVDAKLLLVGDGPEFCTILQLVKNLHIEDRVLFLGKQDNVA  266

Query  243  ELIQLADVVVLSSDREGFPLVMVEALQADKVMASTRVNGVVEWLPN---QYIAEIGDAQG  299
            EL+ ++D+++L S++E F LV++EA+        TRV G+ E + +    Y+ E+GD  G
Sbjct  267  ELLAMSDLMLLLSEKESFGLVLLEAMACGVPCIGTRVGGIPEVIQHGDTGYLCEVGDTTG  326

Query  300  LA  301
            +A
Sbjct  327  VA  328


>Q59002 Uncharacterized glycosyltransferase MJ1607 [Methanocaldococcus 
jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / 
JCM 10045 / NBRC 100440)]
Length=390

 Score = 76.3 bits (186),  Expect = 9e-19
 Identities = 58/179 (32%), Positives = 98/179 (55%), Gaps = 20/179 (11%)

Query  126  VIAVSQALAQGI------PNNKAHVIYNGVYPQP---DLSIEHKQHLLQTIQQDFPQLDS  176
            VI VS++L + +      P +K  VIYNG+ P     +LS E K +  ++I      +  
Sbjct  152  VITVSKSLKEEVCSIFNTPEDKVKVIYNGINPWEFDINLSWEEKINFRRSIG-----VQD  206

Query  177  NKKLVMCIGRLEPVKNISLLIESMQQI----DANLWIIGDGSLRASLEKQVSELNIQNRV  232
            ++K+++ +GRL   K I  LI +M +I    +A L I G G +R  LE    +L ++++V
Sbjct  207  DEKMILFVGRLTYQKGIEYLIRAMPKILERHNAKLVIAGSGDMRDYLEDLCYQLGVRHKV  266

Query  233  AFLGF--RIDARELIQLADVVVLSSDREGFPLVMVEALQADKVMASTRVNGVVEWLPNQ  289
             FLGF      ++L + ADVVV+ S  E F +V +EA+ A   +  + V G++E + ++
Sbjct  267  VFLGFVNGDTLKKLYKSADVVVIPSVYEPFGIVALEAMAAGTPVVVSSVGGLMEIIKHE  325


>Q9R9N1 Lipopolysaccharide core biosynthesis glycosyltransferase 
LpsE [Rhizobium meliloti (strain 1021)]
Length=340

 Score = 74.3 bits (181),  Expect = 3e-18
 Identities = 68/282 (24%), Positives = 129/282 (46%), Gaps = 27/282 (10%)

Query  65   IFLLWQLKSLIQQIQPDIVHAQAGKAAELISRIRPFLPDIKVVTTIHGTKKNKSTYLVGD  124
            I L  ++K + ++ +PD++ A A +A+EL+   +        ++ +       S +   D
Sbjct  65   ILLPLKVKHMARREKPDVLMAWAPRASELMPNYKGAFK----ISRLGDYPTRLSYFRNTD  120

Query  125  AVIAVSQALAQGIPN----NKAHVIYNGVYPQPDLSIEHKQHLLQTIQQDFPQLDSNKKL  180
             ++  +  +A+ + +     +  VI N       ++++  +        D P   ++  +
Sbjct  121  CIVCNTPGIAERVSDLGWKREIRVISNFTGTGRVVAVDRAK-------LDTP---ADAPV  170

Query  181  VMCIGRLEPVKNISLLIESMQQIDA-NLWIIGDGSLRASLEKQVSELNIQNRVAFLGFRI  239
            VM +GR    K    LIE++ ++    LW++GDG  R +L K  ++L +  RV F G++ 
Sbjct  171  VMSMGRFVERKGFHTLIEAVARLPGVYLWLLGDGEERDNLHKLATDLGVSGRVRFAGWQD  230

Query  240  DARELIQLADVVVLSSDREGFPLVMVEALQADKVMASTRVNGVVEWL----PNQYIAEIG  295
            D R  +   DV V+SS  E    V++E+      + STR  G  +W      N  + +IG
Sbjct  231  DTRPFLAAVDVFVMSSSHEPLGNVILESWAQGTPVVSTRSEG-PQWFMRDGENGLMVDIG  289

Query  296  DAQGLAKVIKCALQPEAQNDFSALYAKAKAELTVQAMTEQTL  337
            DA+G A+ I+   Q  A N      A+   E  V   + + +
Sbjct  290  DAEGFARAIE---QIVADNSLRTRLAERGHETLVGQFSREAI  328


>P42982 N-acetyl-alpha-D-glucosaminyl L-malate synthase [Bacillus 
subtilis (strain 168)]
Length=377

 Score = 72.8 bits (177),  Expect = 1e-17
 Identities = 78/339 (23%), Positives = 147/339 (43%), Gaps = 65/339 (19%)

Query  34   ELHVIAH--PCYAEKFNSNVKFHAVD------FARSRWNIFLLWQLKSLIQQIQPDIVHA  85
            E+H I    P     ++ N+ FH V+      F    +++ L  ++  + ++   DI+HA
Sbjct  34   EIHFITSSIPFRLNTYHPNIHFHEVEVNQYAVFKYPPYDLTLASKIAEVAERENLDIIHA  93

Query  86   Q--------AGKAAELISRIRPFLPDIKVVTTIHGTK----------KNKSTYLV--GDA  125
                     A  A +++ R      +I +VTT+HGT           K+   + +   D 
Sbjct  94   HYALPHAVCAYLAKQMLKR------NIGIVTTLHGTDITVLGYDPSLKDLIRFAIESSDR  147

Query  126  VIAVSQALAQGI-----PNNKAHVIYNGVYPQPDL-----SIEHKQHLLQTIQQDFPQLD  175
            V AVS ALA        P  K   IYN +  +  L     +I+ K  +L           
Sbjct  148  VTAVSSALAAETYDLIKPEKKIETIYNFIDERVYLKKNTAAIKEKHGILP----------  197

Query  176  SNKKLVMCIGRLEPVKNISLLIESMQQI----DANLWIIGDGSLRASLEKQVSELNIQNR  231
             ++K+V+ +     VK +  +I   + I     A L ++GDG  +++  + + +  ++++
Sbjct  198  -DEKVVIHVSNFRKVKRVQDVIRVFRNIAGKTKAKLLLVGDGPEKSTACELIRKYGLEDQ  256

Query  232  VAFLGFRIDARELIQLADVVVLSSDREGFPLVMVEALQADKVMASTRVNGVVEWLPNQ--  289
            V  LG +    +L  ++D+ +L S++E F LV++EA+        T + G+ E + N   
Sbjct  257  VLMLGNQDRVEDLYSISDLKLLLSEKESFGLVLLEAMACGVPCIGTNIGGIPEVIKNNVS  316

Query  290  -YIAEIGDAQGLAKVIKCALQPEAQNDFSALYAKAKAEL  327
             ++ ++GD           L+ E     S  + KA  E+
Sbjct  317  GFLVDVGDVTAATARAMSILEDE---QLSNRFTKAAIEM  352


>Q04975 Vi polysaccharide biosynthesis protein VipC/TviE [Salmonella 
typhi]
Length=578

 Score = 59.7 bits (143),  Expect = 3e-13
 Identities = 45/150 (30%), Positives = 66/150 (44%), Gaps = 15/150 (10%)

Query  144  VIYNGVYP---QPDLSIEHKQHLLQTIQQDFPQLDSNKKLVM-----CIGRLEPVKNISL  195
            V+YNGV P   +P   + HK      I Q F Q   +    +      +G   P   I  
Sbjct  366  VVYNGVLPPSTEPSSEVPHK------IWQQFTQKTQDADTTIGGVFRFVGDKNPFAWIDF  419

Query  196  LIESMQQIDANLWI-IGDGSLRASLEKQVSELNIQNRVAFLGFRIDARELIQLADVVVLS  254
                +Q   A  ++ +GDG LRA  +K+  +L I  R+ F+G   D    +Q  +V +L 
Sbjct  420  AARYLQHHPATRFVLVGDGDLRAEAQKRAEQLGILERILFVGASRDVGYWLQKMNVFILF  479

Query  255  SDREGFPLVMVEALQADKVMASTRVNGVVE  284
            S  EG P V++EA      + ST   G  E
Sbjct  480  SRYEGLPNVLIEAQMVGVPVISTPAGGSAE  509


>Q0P9C5 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undecaprenol 
alpha-1,4-N-acetyl-D-galactosaminyltransferase [Campylobacter 
jejuni subsp. jejuni serotype O:2 (strain ATCC 
700819 / NCTC 11168)]
Length=359

 Score = 58.5 bits (140),  Expect = 6e-13
 Identities = 46/144 (32%), Positives = 75/144 (52%), Gaps = 12/144 (8%)

Query  178  KKLVMCIGRLEPVKNISLLIESMQQIDANLW------IIGDGSLRASLEKQVSELNIQNR  231
            + LV+ IGRL+  KN  + ++++  +D NL       I GDG LR  LE +V  L I  +
Sbjct  182  ENLVLFIGRLDHNKNPVMFLKAIAHLDKNLQENYKFVIAGDGQLRQELEYKVKSLGI--K  239

Query  232  VAFLGFRIDARELIQLADVVVLSSDREGFPLVMVEALQADKV-MASTRVNGVVEWLPNQY  290
            V FLG   + + L + A V+ L S  EG P V++E+L  +   ++S+  NG  + + + +
Sbjct  240  VDFLGRVENVKALYEKAKVLCLCSFVEGLPTVLIESLYFEVCRISSSYYNGAKDLIKDNH  299

Query  291  ---IAEIGDAQGLAKVIKCALQPE  311
               +    D   LAK ++  L  E
Sbjct  300  DGLLVGCDDEIALAKKLELVLNDE  323


>Q58459 Uncharacterized glycosyltransferase MJ1059 [Methanocaldococcus 
jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / 
JCM 10045 / NBRC 100440)]
Length=406

 Score = 56.2 bits (134),  Expect = 4e-12
 Identities = 34/118 (29%), Positives = 66/118 (56%), Gaps = 5/118 (4%)

Query  165  QTIQQDFPQLDSNKKLVMCIGRLEPVKNISLLIESMQQI-----DANLWIIGDGSLRASL  219
            + +++ +  +  +  + + IGRL   K    LI S +++     +A L I+GDG L+  L
Sbjct  189  EPLEKQYRNIFKDSFVFINIGRLTEQKGQWFLIRSFKRVTEKYPNAKLIILGDGELKNKL  248

Query  220  EKQVSELNIQNRVAFLGFRIDARELIQLADVVVLSSDREGFPLVMVEALQADKVMAST  277
            ++ +++LN+QN V  LG + +  + ++ ++  V SS  EG P  ++EAL  +  + ST
Sbjct  249  QELINKLNLQNNVYLLGMQKNPFKFLKHSNCFVFSSLWEGLPNTVIEALSLNLPVIST  306


>Q0P9C7 N-acetylgalactosamine-N,N'-diacetylbacillosaminyl-diphospho-undecaprenol 
4-alpha-N-acetylgalactosaminyltransferase 
[Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 
700819 / NCTC 11168)]
Length=365

 Score = 55.5 bits (132),  Expect = 6e-12
 Identities = 70/295 (24%), Positives = 123/295 (42%), Gaps = 20/295 (7%)

Query  3    MKIVQILATSGGLGGLEQHTFNLTNELALSQELHVIAHPCYAEKFNSNVKFHAVDFARSR  62
            M+ + I   S G GG E+    L   L+L  E+H+I             + H ++ ++  
Sbjct  1    MQKLGIFIYSLGSGGAERVVATLLPILSLKFEVHLILMNDKISYEIPECQIHFLECSKPS  60

Query  63   WNI--------FLLWQLKSLIQQIQPDIVHAQAGKAAELISRIRPFLPDIKVVTTIHGTK  114
             N         FL  + K L + +  D       +   +    R F    ++V     T 
Sbjct  61   ENPILKFLKLPFLALKYKKLCRNLGIDTEFVFLNRPNYIALMARMFGNKTRLVINECTTP  120

Query  115  -----KNKSTYLVGDAVIAVSQALAQGI-PNNKAHV---IYNGVYPQPDLSIEHKQHLLQ  165
                 KN    LV   +I++    A  I PN+K ++   + N         I +    L+
Sbjct  121  SVMYMKNNFNSLVNKFLISLLYPKADLILPNSKGNLEDLVQNFSISPKKCEILYNAIDLE  180

Query  166  TI-QQDFPQLDSNKKLVMCIGRLEPVKNISLLIESMQQI--DANLWIIGDGSLRASLEKQ  222
             I Q+    +    K ++ +GRL+  KN +LLI +  ++  D  L I+G+G L+  L   
Sbjct  181  NIGQKALEDIALKDKFILSVGRLDKGKNHALLIRAYARLKTDLKLVILGEGVLKDELLAL  240

Query  223  VSELNIQNRVAFLGFRIDARELIQLADVVVLSSDREGFPLVMVEALQADKVMAST  277
            + ELN++ +V  LGF  +  + +   +    +S  EGF  V++E+L     +  T
Sbjct  241  IKELNLEEKVLLLGFDNNPYKYMAKCEFFAFASVFEGFSNVLIESLACSCAVVCT  295


>P71053 Putative glycosyltransferase EpsD [Bacillus subtilis (strain 
168)]
Length=381

 Score = 55.5 bits (132),  Expect = 6e-12
 Identities = 79/311 (25%), Positives = 131/311 (42%), Gaps = 49/311 (16%)

Query  34   ELHVIAH-----PCYAEKFNSNVKFHAVDFARSRWNIFLLWQLKSLIQQIQPDIVHAQA-  87
            E+HV A+     P   EKF+  ++    D      N+ +  QLK +I   + DIVH    
Sbjct  32   EVHVAANGQTKLPYVDEKFSIPIRRSPFD----PQNLAVYRQLKKVIDTYEYDIVHCHTP  87

Query  88   --GKAAELISRIRPFLPDIKVVTTIHG-----------------TKKNKSTYLVGDAVIA  128
              G  A L +R +      KV+ T HG                  +K  S Y   D +I 
Sbjct  88   VGGVLARLAAR-QARRHGTKVLYTAHGFHFCKGAPMKNWLLYYPVEKWLSAYT--DCLIT  144

Query  129  VSQA---LAQGI--PNNKAHVIYN-GVYPQPDLSIEHKQHLLQTIQQDFPQLDSNKKLVM  182
            +++     A+G+  P  +   I+  GV  +    +   +      +  F + D    +++
Sbjct  145  INEEDYIRAKGLQRPGGRTQKIHGIGVNTERFRPVSPIEQQRLREKHGFREDDF---ILV  201

Query  183  CIGRLEPVKNISLLIESMQQI-----DANLWIIGDGSLRASLEKQVSELNIQNRVAFLGF  237
                L   KN   LIE+   +        L   G+G++  + +    +L     V F GF
Sbjct  202  YPAELNLNKNQKQLIEAAALLKEKIPSLRLVFAGEGAMEHTYQTLAEKLGASAHVCFYGF  261

Query  238  RIDARELIQLADVVVLSSDREGFPLVMVEALQADKVMASTRVNG---VVEWLPNQYIAEI  294
              D  ELIQLADV V SS REG  + ++E + A++   +T   G   ++    N ++ +I
Sbjct  262  CSDIHELIQLADVSVASSIREGLGMNVLEGMAAEQPAIATDNRGHREIIRDGENGFLIKI  321

Query  295  GDAQGLAKVIK  305
            GD+   A+ I+
Sbjct  322  GDSAAFARRIE  332


>Q0P9C9 N,N'-diacetylbacillosaminyl-diphospho-undecaprenol alpha-1,3-N-acetylgalactosaminyltransferase 
[Campylobacter jejuni 
subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)]
Length=376

 Score = 49.3 bits (116),  Expect = 6e-10
 Identities = 43/139 (31%), Positives = 65/139 (47%), Gaps = 7/139 (5%)

Query  178  KKLVMCIGRLEPVKNISLLIES--MQQIDANLWIIGDGSLRASLEKQVSELNIQNRVAFL  235
            K +V+ I R    K +    ES  M +  AN  ++G      S      E      V +L
Sbjct  203  KPIVLMIARALWHKGVKEFYESATMLKDKANFVLVGGRDENPSCASL--EFLNSGAVHYL  260

Query  236  GFRIDARELIQLADVVVLSSDREGFPLVMVEALQADKVMASTRVNGVVEWLPNQY---IA  292
            G R D  EL+Q  D+ VL S +EGFP+ ++EA    K +  +   G VE + N Y    A
Sbjct  261  GARSDIVELLQNCDIFVLPSYKEGFPVSVLEAKACGKAIVVSDCEGCVEAISNAYDGLWA  320

Query  293  EIGDAQGLAKVIKCALQPE  311
            +  +A+ L++ I   L+ E
Sbjct  321  KTKNAKDLSEKISLLLEDE  339


>P27127 Lipopolysaccharide 1,6-galactosyltransferase [Escherichia 
coli (strain K12)]
Length=359

 Score = 48.5 bits (114),  Expect = 1e-09
 Identities = 54/215 (25%), Positives = 96/215 (45%), Gaps = 31/215 (14%)

Query  71   LKSLIQQIQPDIVHAQAGKAAELISRIRPFLP-DIKVVTTIHGT---KKNKSTYLVGDAV  126
            L   +Q+ QPDIV      +    ++ R     D+ V +  H +   KK+       D  
Sbjct  76   LSKWLQEYQPDIVICIDVISCLFAAKARKKSGIDMPVFSWPHFSLDHKKHAEYITCADYH  135

Query  127  IAVSQALAQ-----GIPNNKAHVIYNGVYPQPDLSIEHKQHLLQTIQQDFPQLDSNKKLV  181
            +A+S  + Q     G+  +  +VI+N V        E K  ++   ++            
Sbjct  136  LAISSGIKQQMINRGVAESTINVIFNPV--------ETKDSVIPAPEE------GETATF  181

Query  182  MCIGRL--EPVKNISLLIESMQQIDAN--LWIIGDGSLRASLEKQVSELNIQNRVAFLGF  237
            + +GR+  E  K +  L++ + Q   N  L ++GDGS     +    ELNI +R+ + G+
Sbjct  182  IYVGRMKFEGQKRVKDLLDGLSQAKGNWKLHVLGDGSDFEKCQAYGRELNIDDRIVWYGW  241

Query  238  RIDARELIQ----LADVVVLSSDREGFPLVMVEAL  268
            +    EL+Q        ++L+S  EGFP+ ++EAL
Sbjct  242  QQYPWELVQQDIEKVSALLLTSSFEGFPMTLLEAL  276


>P71055 Putative glycosyltransferase EpsF [Bacillus subtilis (strain 
168)]
Length=384

 Score = 48.1 bits (113),  Expect = 1e-09
 Identities = 35/132 (27%), Positives = 65/132 (49%), Gaps = 9/132 (7%)

Query  141  KAHVIYNGVYPQPDLSIEHKQHLLQTIQQDFPQLDSNKKLVMCIGRLEPVKNISLLIE--  198
            + H++ NG+    DL +          ++    + +++ ++  + R   VKN + L++  
Sbjct  171  RVHLLPNGI----DLELFAPNGQAADEEKAARGIAADRLIIGHVARFHEVKNHAFLLKLA  226

Query  199  ---SMQQIDANLWIIGDGSLRASLEKQVSELNIQNRVAFLGFRIDARELIQLADVVVLSS  255
                 + I   L + GDG L   +E++  + N+ + V FLG      EL++  DV V+ S
Sbjct  227  AHLKERGIRFQLVLAGDGPLCGEIEEEARQQNLLSDVLFLGTEERIHELMRTFDVFVMPS  286

Query  256  DREGFPLVMVEA  267
              EG P+V+VEA
Sbjct  287  LYEGLPVVLVEA  298



Lambda      K        H        a         alpha
   0.322    0.135    0.387    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 1239600


  Database: 50f93a689cb497df52363f44c1996950.SwissProt.fasta
    Posted date:  May 19, 2024  7:43 PM
  Number of letters in database: 4,696
  Number of sequences in database:  12



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40