BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 7b4a06566d328942ef1243b1e36c9acb.SwissProt.fasta
15 sequences; 7,603 total letters
Query= ACIAD0644
Length=429
Score E
Sequences producing significant alignments: (Bits) Value
P08192 Dihydrofolate synthase/folylpolyglutamate synthase [Escher... 312 2e-106
P43775 Dihydrofolate synthase/folylpolyglutamate synthase [Haemop... 256 3e-84
Q89AT2 Dihydrofolate synthase/folylpolyglutamate synthase [Buchne... 240 2e-78
Q8K9X3 Dihydrofolate synthase/folylpolyglutamate synthase [Buchne... 233 9e-76
P57265 Dihydrofolate synthase/folylpolyglutamate synthase [Buchne... 210 8e-67
Q05865 Dihydrofolate synthase/folylpolyglutamate synthase [Bacill... 160 7e-48
P15925 Folylpolyglutamate synthase [Lacticaseibacillus casei] 159 1e-47
Q9UTD0 Probable dihydrofolate synthetase [Schizosaccharomyces pom... 146 1e-42
Q9HS44 Probable folylpolyglutamate synthase / Probable dihydropte... 144 2e-40
D4GW05 Probable folylpolyglutamate synthase / Probable dihydropte... 143 4e-40
Q12676 Dihydrofolate synthetase [Saccharomyces cerevisiae (strain... 139 4e-40
F4JYE9 Dihydrofolate synthetase [Arabidopsis thaliana] 125 1e-34
I6Y0R5 Dihydrofolate synthase/folylpolyglutamate synthase [Mycoba... 125 1e-34
Q8W035 Folylpolyglutamate synthase [Arabidopsis thaliana] 122 9e-34
F4J2K2 Folylpolyglutamate synthase [Arabidopsis thaliana] 115 6e-31
>P08192 Dihydrofolate synthase/folylpolyglutamate synthase [Escherichia
coli (strain K12)]
Length=422
Score = 312 bits (800), Expect = 2e-106
Identities = 181/421 (43%), Positives = 253/421 (60%), Gaps = 13/421 (3%)
Query 6 PSIQDDLMTWLTYWGHVHVTGIDLGLERVIPVAEKLGVTQTSAKVFTVAGTNGKGSTTTT 65
P L +WL+Y ++H IDLGLERV VA +LGV + + VFTVAGTNGKG+T T
Sbjct 7 PQAASPLASWLSYLENLHSKTIDLGLERVSLVAARLGVLKPAPFVFTVAGTNGKGTTCRT 66
Query 66 LASILTEQGYRVGLYQSPHIYRFNERVKLNGQEASDQQLIEAFVKVDQVRRECGLSLSFF 125
L SIL GY+VG+Y SPH+ R+ ERV++ GQE + +F +++ R + +SL++F
Sbjct 67 LESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPESAHTASFAEIESARGD--ISLTYF 124
Query 126 EATTLAAFLIFQQQACDIWVLEVGLGGRLDVVNVIDPDVAVITNIGLDHTDWLGDTIEKI 185
E TL+A +F+Q D+ +LEVGLGGRLD N++D DVAV+T+I LDHTDWLG E I
Sbjct 125 EYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVDADVAVVTSIALDHTDWLGPDRESI 184
Query 186 AFEKAGIIRSNIPVIFGGVQTLPQAIADKAAAQQAQLYVLGRDYFYQVAPDQKSWNFASS 245
EKAGI RS P I G + +P IAD A + A L G ++ Y V W F+ +
Sbjct 185 GREKAGIFRSEKPAIVGEPE-MPSTIADVAQEKGALLQRRGVEWNYSVT--DHDWAFSDA 241
Query 246 -GTTLKLPLGSLALENISTAVAALLVSGIDVSQAAITSGIEKAHLAGRFEQRIIKNKAVV 304
GT LPL + N +TA+AAL SG++VS+ AI GI A L GRF Q + ++ V+
Sbjct 242 HGTLENLPLPLVPQPNAATALAALRASGLEVSENAIRDGIASAILPGRF-QIVSESPRVI 300
Query 305 FDAGHNGHGVDFLLKQLRNFLEYNKQYTEVIAVFSMLADKDIKAVVNLLKPAVLHWKVAA 364
FD HN H ++L +++ + + V+AV ML DKDI + LK V W A
Sbjct 301 FDVAHNPHAAEYLTGRMKALPKNGR----VLAVIGMLHDKDIAGTLAWLKSVVDDWYCAP 356
Query 365 LSVPRAAPVTQLLDALDGEITQQYDSIREAFSVALEESRPHQLILVCGSFHTLEAVWEYL 424
L PR A QLL+ L + +DS+ +A+ A+ +++ +LVCGSFHT+ V E +
Sbjct 357 LEGPRGATAEQLLEHLGN--GKSFDSVAQAWDAAMADAKAEDTVLVCGSFHTVAHVMEVI 414
Query 425 E 425
+
Sbjct 415 D 415
>P43775 Dihydrofolate synthase/folylpolyglutamate synthase [Haemophilus
influenzae (strain ATCC 51907 / DSM 11121 / KW20 /
Rd)]
Length=437
Score = 256 bits (654), Expect = 3e-84
Identities = 159/438 (36%), Positives = 233/438 (53%), Gaps = 36/438 (8%)
Query 12 LMTWLTYWGHVHVTGIDLGLERVIPVAEKLGVTQTSAKVFTVAGTNGKGSTTTTLASILT 71
L WL+Y H IDLGL+R+ VAEKL + V TV GTNGKG+T L +IL
Sbjct 12 LAEWLSYLEKSHFKPIDLGLDRIKSVAEKLDLLHPVPYVITVGGTNGKGTTCRLLETILL 71
Query 72 EQGYRVGLYQSPHIYRFNERVKLNGQEASDQQLIEAFVKVDQVRRECGLSLSFFEATTLA 131
G RVG+Y SPH+ R+NERV++ Q+ D+ +F +D+ + E SL++FE +TL+
Sbjct 72 NHGLRVGVYSSPHLLRYNERVRIQNQDLPDEAHTASFAFIDENKTE---SLTYFEFSTLS 128
Query 132 AFLIFQQQACDIWVLEVGLGGRLDVVNVIDPDVAVITNIGLDHTDWLGDTIEKIAFEKAG 191
A +F+Q D+ +LEVGLGGRLD N++D +AVIT+I +DHTD+LGDT E IAFEKAG
Sbjct 129 ALHLFKQAKLDVVILEVGLGGRLDATNIVDSHLAVITSIDIDHTDFLGDTREAIAFEKAG 188
Query 192 IIRSNIPVIFGGVQTLPQAIADKAAAQQAQLYVLGRDYFYQVAPDQKSWNFASSGTTLK- 250
I R N PV+ G +PQ + D+A Q V RD + + ++W + + L+
Sbjct 189 IFRENCPVVI-GEPNVPQTMLDQAEKLHCQ--VARRDVDWLFEQNAENWQWQNKKVRLEN 245
Query 251 LPLGSLALENISTAVAALLVSGIDVSQAAITSGIEKAHLAGRFEQRIIKNK--------- 301
LP + L N +T +AA+ D+S+ + +++ L GRF Q I +K
Sbjct 246 LPFCQIPLANAATVLAAVQYLPFDISEQTLRKSLQEVELVGRF-QAIKTDKREKLADYLG 304
Query 302 -------AVVFDAGHNGHGVDFL---LKQLRNFLEYNKQYTEVIAVFSMLADKDIKAVVN 351
++ D GHN H +L L L+ +E ++IAV ML DKD V
Sbjct 305 VPVETLPTIIVDVGHNPHAAKYLSEKLTALKRSIE-----GKMIAVCGMLKDKDANGVFE 359
Query 352 LLKPAVLHWKVAALSVPRAAPVTQLLDALDGEI----TQQYDSIREAFSVALEESRPHQL 407
L P + W L R +L++ L +S+ + AL+ + + +
Sbjct 360 HLTPIIDEWHCVTLGGYRGQSGDELVEKLKSHFPNIQATSDNSVMDGVCTALKSAVKNDV 419
Query 408 ILVCGSFHTLEAVWEYLE 425
+LV GSFHT+ W +E
Sbjct 420 VLVFGSFHTVAEFWAVVE 437
>Q89AT2 Dihydrofolate synthase/folylpolyglutamate synthase [Buchnera
aphidicola subsp. Baizongia pistaciae (strain Bp)]
Length=426
Score = 240 bits (613), Expect = 2e-78
Identities = 151/408 (37%), Positives = 229/408 (56%), Gaps = 12/408 (3%)
Query 12 LMTWLTYWGHVHVTGIDLGLERVIPVAEKLGVTQTSAKVFTVAGTNGKGSTTTTLASILT 71
L WL Y H + ID L RV VA+KLGV ++ A VF V GTNGKGST L ++L
Sbjct 12 LYEWLYYLDHFMLDNIDPTLNRVFYVAKKLGVLKSKAFVFIVGGTNGKGSTCHVLENLLL 71
Query 72 EQGYRVGLYQSPHIYRFNERVKLNGQEASDQQLIEAFVKVDQVRRECGLSLSFFEATTLA 131
GYRVGLY SPH+ R+ ERV++NG E I AF V + + + L+ FE TL+
Sbjct 72 NSGYRVGLYTSPHLMRYTERVRINGFELEHLYHISAFNDVKYFQND--VLLTRFEFITLS 129
Query 132 AFLIFQQQACDIWVLEVGLGGRLDVVNVIDPDVAVITNIGLDHTDWLGDTIEKIAFEKAG 191
A ++F+ DI +LEVGLGGRLD N++ DV+VITNI +DH+ LG I+ EK+G
Sbjct 130 ALILFKSYNLDIIILEVGLGGRLDATNILSADVSVITNIDIDHSKILGVNRSSISVEKSG 189
Query 192 IIRSNIPVIFGGVQTLPQAIADKAAAQQAQLYVLGRDYFYQVAPDQKSWNFASSGTT-LK 250
I R N I P+ A ++ +L ++ D+ Y+ + W+F SS T L
Sbjct 190 IFRKNKIAIVAD-NNFPKVAQYLAKKKKVRLRIVNIDWIYKKI--EFEWSFCSSKITWLH 246
Query 251 LPL-GSLALENISTAVAALLVSGIDVSQAAITSGIEKAHLAGRFEQRIIKNKAVVFDAGH 309
LPL +++L++++TA++A+ SGI ++Q S I + L GRFE I N ++ D H
Sbjct 247 LPLPRNVSLDSVATALSAVSESGIKINQKVFRSCISEITLCGRFE-TISYNPIIILDVAH 305
Query 310 NGHGVDFLLKQLRNFLEYNKQYTEVIAVFSMLADKDIKAVVNLLKPAVLHWKVAALSVPR 369
N H +L K++ +F K+ + AV +L +K+IK +V+ L P V +W L R
Sbjct 306 NPHSARYLFKKMSSF----KKNGNIFAVVGILKEKNIKDIVSPLIPIVDYWYCITLLTHR 361
Query 370 AAPVTQLLDALDGEITQQYDSIREAFSVALEESRPHQLILVCGSFHTL 417
+A ++++ L +Q ++ A ++ + ++L+ GSF T+
Sbjct 362 SATSSEIIKYLPNHNSQISKNMTVALEKIFDKVTNNDIVLIFGSFITV 409
>Q8K9X3 Dihydrofolate synthase/folylpolyglutamate synthase [Buchnera
aphidicola subsp. Schizaphis graminum (strain Sg)]
Length=418
Score = 233 bits (595), Expect = 9e-76
Identities = 140/383 (37%), Positives = 217/383 (57%), Gaps = 12/383 (3%)
Query 37 VAEKLGVTQTSAKVFTVAGTNGKGSTTTTLASILTEQGYRVGLYQSPHIYRFNERVKLNG 96
+A+KLG+ + FTV GTNGKG+T L +L + GY+VGLY SPH+ ++ER+K+NG
Sbjct 32 IAKKLGLLNLKSFFFTVGGTNGKGTTCAMLEKLLLDSGYQVGLYTSPHLINYSERIKVNG 91
Query 97 QEASDQQLIEAFVKVDQVRRECGLSLSFFEATTLAAFLIFQQQACDIWVLEVGLGGRLDV 156
S++ I +F+ +D + +SL++FE TL+A +F Q + DI +LEVGLGGRLD
Sbjct 92 LYLSEKDHIFSFLIIDAEKGN--VSLTYFEFITLSALFLFSQYSLDIIILEVGLGGRLDA 149
Query 157 VNVIDPDVAVITNIGLDHTDWLGDTIEKIAFEKAGIIR-SNIPVIFGGVQTLPQAIADKA 215
N+ID D++VITNIG+DHT LG I EK+GI R I VI G + +P ++ + A
Sbjct 150 TNIIDSDLSVITNIGIDHTSCLGTDRISIGREKSGIFRKGKIAVI--GEKNIPISVDEIA 207
Query 216 AAQQAQLYVLGRDYFYQVAPDQKSWNFASSGTTL-KLPLGSLALENISTAVAALLVSGID 274
++ L + D+F+ SWNF S L LP+ + L N + A+A+L SG++
Sbjct 208 KEKKTILKKIDVDWFW-TRTKIDSWNFIHSNIELYNLPVSRIPLSNTAIALASLFYSGLE 266
Query 275 VSQAAITSGIEKAHLAGRFEQRIIKNKAVVFDAGHNGHGVDFLLKQLRNFLEYNKQYTEV 334
V+ + S I K L+GRF Q + + ++ D HN H +L +++ ++
Sbjct 267 VNLKKLKSSISKVQLSGRF-QTVFNSPRIILDVAHNVHAALYLSEKIDEI----DTEGQI 321
Query 335 IAVFSMLADKDIKAVVNLLKPAVLHWKVAALSVPRAAPVTQLLDALDGEITQQYDSIREA 394
AVF +L DKD+ VV +L+ + +W V L R+A + L L +++I E+
Sbjct 322 YAVFGILKDKDVAGVVQVLQKKINYWYVVNLKTNRSASINYLKKKLSLNNALFFNNINES 381
Query 395 FSVALEESRPHQLILVCGSFHTL 417
+ + +ILV GSF T+
Sbjct 382 WQAIKKVITKKDIILVFGSFFTV 404
>P57265 Dihydrofolate synthase/folylpolyglutamate synthase [Buchnera
aphidicola subsp. Acyrthosiphon pisum (strain APS)]
Length=411
Score = 210 bits (534), Expect = 8e-67
Identities = 143/408 (35%), Positives = 216/408 (53%), Gaps = 14/408 (3%)
Query 12 LMTWLTYWGHVHVTGIDLGLERVIPVAEKLGVTQTSAKVFTVAGTNGKGSTTTTLASILT 71
L WL Y + I L + +A+KLG+ ++ + +FTVAGTNGKG+T L +L
Sbjct 8 LSLWLKYLEQLDKKRI-YNLTELKFLAKKLGLLKSESFIFTVAGTNGKGTTCAVLERLLL 66
Query 72 EQGYRVGLYQSPHIYRFNERVKLNGQEASDQQLIEAFVKVDQVRRECGLSLSFFEATTLA 131
+ GY+VGLY SPH+ F ERV++NG +++ I++F V+ VR G+ L++FE TLA
Sbjct 67 DSGYQVGLYTSPHLINFVERVRINGFVLHEEEHIDSFQNVELVRN--GVLLTYFEFITLA 124
Query 132 AFLIFQQQACDIWVLEVGLGGRLDVVNVIDPDVAVITNIGLDHTDWLGDTIEKIAFEKAG 191
A ++F++ + D +L+VGLGGRLD N+ID D+++ITNIG+DHT LG IA EK G
Sbjct 125 ALILFKRYSLDCIILKVGLGGRLDATNIIDSDISIITNIGIDHTSILGRDRISIAREKCG 184
Query 192 IIRSN-IPVIFGGVQTLPQAIADKAAAQQAQLYVLGRDYFYQVAPDQKSWNFASSGTTL- 249
+ R N I VI G +P ++ A ++ L + D+ ++ + WNF S L
Sbjct 185 VFRKNKISVI--GETDIPCSMYQIAKEKKTILKKIDIDWSWE--KKRNYWNFFHSTIQLY 240
Query 250 KLPLGSLALENISTAVAALLVSGIDVSQAAITSGIEKAHLAGRFEQRIIKNKAVVFDAGH 309
LP + L + +TA++ L S + + I I L GRF Q I ++ D H
Sbjct 241 NLPETQVPLSSAATALSTLYYSRFKIKEKIIRKSISNVQLPGRF-QVISTFPYIIVDVAH 299
Query 310 NGHGVDFLLKQLRNFLEYNKQYTEVIAVFSMLADKDIKAVVNLLKPAVLHWKVAALSVPR 369
N + +L +++ K Y AV +L DKDI +++ L + HW A L R
Sbjct 300 NPNAAFYLSQKIDEINITGKIY----AVVGILKDKDILGIIDPLANKIHHWFTAPLKTIR 355
Query 370 AAPVTQLLDALDGEITQQYDSIREAFSVALEESRPHQLILVCGSFHTL 417
A +L T SI A+ AL + I++ GSF T+
Sbjct 356 TATKHELKKFFPIHNTSILKSIEIAYKKALILVKKEDAIIIFGSFLTV 403
>Q05865 Dihydrofolate synthase/folylpolyglutamate synthase [Bacillus
subtilis (strain 168)]
Length=430
Score = 160 bits (405), Expect = 7e-48
Identities = 128/422 (30%), Positives = 206/422 (49%), Gaps = 32/422 (8%)
Query 26 GIDLGLERVIPVAEKLGVTQTSAKVFTVAGTNGKGSTTTTLASILTEQGYRVGLYQSPHI 85
G+ GL R+ + +LG + + F VAGTNGKGST + S+L E GY VG + SP+I
Sbjct 19 GVKPGLGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYI 78
Query 86 YRFNERVKLNGQEASDQ-------QLIEAFVKVDQVRRECGLSLSFFEATTLAAFLIFQQ 138
FNER+ +NG SD+ Q+ +DQ E G FE T AFL F +
Sbjct 79 ITFNERISVNGIPISDEEWTALVNQMKPHVEALDQT--EYGQPTE-FEIMTACAFLYFAE 135
Query 139 -QACDIWVLEVGLGGRLDVVNVIDPDVAVITNIGLDHTDWLGDTIEKIAFEKAGIIRSNI 197
D + E GLGGR D NV++P + VIT+IG DH + LG+TIE+IA EKAGII+ I
Sbjct 136 FHKVDFVIFETGLGGRFDSTNVVEPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGI 195
Query 198 PVIFGGVQTLPQAI------ADKAAAQQAQLYVLGRDYFYQVAPDQKSWNFASSGTTLK- 250
P++ Q P+A+ A++ AA L+ + + P + ++F + +
Sbjct 196 PIVTAVTQ--PEALQVIRHEAERHAAPFQSLHDACVIFNEEALPAGEQFSFKTEEKCYED 253
Query 251 ---LPLGSLALENISTAVAA---LLVSGI-DVSQAAITSGIEKAHLAGRFEQRIIKNKAV 303
+G+ +N + ++ A L I +S A+ SG+ KA GR E + ++ V
Sbjct 254 IRTSLIGTHQRQNAALSILAAEWLNKENIAHISDEALRSGLVKAAWPGRLE-LVQEHPPV 312
Query 304 VFDAGHNGHGVDFLLKQLRNFLEYNKQYTEVIAVFSMLADKDIKAVVNLLKPAVLHWKVA 363
D HN GV+ L + ++ ++ + VFS L DK + ++ L+ A
Sbjct 313 YLDGAHNEEGVEKLAETMKQRFANSR----ISVVFSALKDKPYQNMIKRLETIAHAIHFA 368
Query 364 ALSVPRAAPVTQLLDALDGEITQQYDSIREAFSVALEESRPHQLILVCGSFHTLEAVWEY 423
+ PRA+ L DA + + + + ++++L+ GS + + + +
Sbjct 369 SFDFPRASLAKDLYDASEISNKSWSEDPDDVIKFIESKKGSNEIVLITGSLYFISDIRKR 428
Query 424 LE 425
L+
Sbjct 429 LK 430
Score = 25.8 bits (55), Expect = 0.038
Identities = 45/218 (21%), Positives = 83/218 (38%), Gaps = 29/218 (13%)
Query 2 KLDAPSIQDDLMTWLTYWGHVHVTGIDLGLERVIPVAEKLGVTQTSAKVFTVAGTNGKGS 61
+ D+ ++ + L+T +T GH H+ + +E + EK G+ + + T +
Sbjct 151 RFDSTNVVEPLLTVITSIGHDHMNILGNTIEEI--AGEKAGIIKEGIPIVTAV------T 202
Query 62 TTTTLASILTEQGYRVGLYQSPH--IYRFNERVKLNGQEASDQQLIEAFVKV------DQ 113
L I E +QS H FNE G++ S + + + +
Sbjct 203 QPEALQVIRHEAERHAAPFQSLHDACVIFNEEALPAGEQFSFKTEEKCYEDIRTSLIGTH 262
Query 114 VRRECGLSLSFFEATTLAAFLIFQQQACDIWVLEVGLGGRLDVVNVIDPDVAVITNIGLD 173
R+ LS+ E +A +++ GRL++V P + LD
Sbjct 263 QRQNAALSILAAEWLNKENIAHISDEALRSGLVKAAWPGRLELVQEHPP-------VYLD 315
Query 174 --HTDWLGDTIEKIA-FEKAGIIRSNIPVIFGGVQTLP 208
H + + +EK+A K S I V+F ++ P
Sbjct 316 GAHNE---EGVEKLAETMKQRFANSRISVVFSALKDKP 350
>P15925 Folylpolyglutamate synthase [Lacticaseibacillus casei]
Length=428
Score = 159 bits (403), Expect = 1e-47
Identities = 128/413 (31%), Positives = 192/413 (46%), Gaps = 34/413 (8%)
Query 33 RVIPVAEKLGVTQTSAKVFTVAGTNGKGSTTTTLASILTEQGYRVGLYQSPHIYRFNERV 92
R++ + LG Q + V GTNGKGS +A +L G VGLY SP I RFNER+
Sbjct 24 RILTLLHALGNPQQQGRYIHVTGTNGKGSAANAIAHVLEASGLTVGLYTSPFIMRFNERI 83
Query 93 KLNGQEASDQQLIEA--FVKVDQVR---RECGLSLSFFEATTLAAFLIFQQQACDIWVLE 147
++ + D L+ A FV+ R ++ +++ FE T + F+Q+ D+ V+E
Sbjct 84 MIDHEPIPDAALVNAVAFVRAALERLQQQQADFNVTEFEFITALGYWYFRQRQVDVAVIE 143
Query 148 VGLGGRLDVVNVIDPDVAVITNIGLDHTDWLGDTIEKIAFEKAGIIRSNIPVIFGG-VQT 206
VG+GG D NVI P V+V+T + LDH LG TI IA KAGII+ IPV+ G V
Sbjct 144 VGIGGDTDSTNVITPVVSVLTEVALDHQKLLGHTITAIAKHKAGIIKRGIPVVTGNLVPD 203
Query 207 LPQAIADKAAAQQAQLYVLGRDYFYQVAPDQKSWNFASSGT---------TLKLPL-GSL 256
+A K A +Q RD+ P K + T L++PL G
Sbjct 204 AAAVVAAKVATTGSQWLRFDRDF---SVPKAKLHGWGQRFTYEDQDGRISDLEVPLVGDY 260
Query 257 ALENISTAVAALLV----SGIDVSQAAITSGIEKAHLAGRFEQRIIKNKAVVFDAGHNGH 312
N++ A+ V + ++ I G+ +H R E +I +V D HN
Sbjct 261 QQRNMAIAIQTAKVYAKQTEWPLTPQNIRQGLAASHWPARLE-KISDTPLIVIDGAHNPD 319
Query 313 GVDFLLKQLRNFLEYNKQYTEVIAVFSMLADKDIKAVVNLLKPAV-LHWKVAALSVPRAA 371
G++ L+ L+ + + +LADKD A+ + L A + V PRA
Sbjct 320 GINGLITALKQLFS-----QPITVIAGILADKDYAAMADRLTAAFSTVYLVPVPGTPRAL 374
Query 372 PVTQLLDALDGEITQQYDSIREAFSVALEESRPHQLILVCGSFHTLEAVWEYL 424
P +G + DS +EA + +L + P Q I++ GS + AV + L
Sbjct 375 PEAGYEALHEGRLK---DSWQEALAASLNDV-PDQPIVITGSLYLASAVRQTL 423
>Q9UTD0 Probable dihydrofolate synthetase [Schizosaccharomyces
pombe (strain 972 / ATCC 24843)]
Length=417
Score = 146 bits (368), Expect = 1e-42
Identities = 119/423 (28%), Positives = 205/423 (48%), Gaps = 35/423 (8%)
Query 27 IDLGLERVIPVAEKLGVTQTSAKVFTVAGTNGKGSTTTTLASILTEQGYRVGLYQSPHIY 86
I LGL+R++ + + LG Q S +AGTNGKGS + + + L + + G Y SPH
Sbjct 3 IQLGLQRMLQLLKHLGNPQESFCAVQIAGTNGKGSICSYIYTSLLQAAIKTGRYTSPHFL 62
Query 87 RFNERVKLNGQEASDQQLIEAFVKVDQVRRECGLSLSFFEATTLAAFLIFQQQACDIWVL 146
+ + +NGQ AS++ + +V +V R + FE T AF F + V+
Sbjct 63 EPRDTISINGQIASEEIFNTCWKQVIEVDRRFRTKATEFELLTATAFQCFHHSGVRVAVI 122
Query 147 EVGLGGRLDVVNVI-DPDVAVITNIGLDHTDWLGDTIEKIAFEKAGIIRSNIPVIFGGVQ 205
E G+GGRLD NV +P +++I+ I LDH +LG+T+E IA EKAGI + N+P + G+
Sbjct 123 ETGMGGRLDATNVFEEPVLSIISRICLDHQAFLGNTLEAIAKEKAGIFKKNVPCVVDGLN 182
Query 206 TLPQAIADKAAAQQAQLYVLGRDYFY----QVAPDQKSW--NFASSGT-TLKLPL-GSLA 257
+ K +A++ + + FY + ++ W N + GT T PL G
Sbjct 183 EVNVLNQLKLSAEETRAHP-----FYLAKGKSGENKNEWIINTPNWGTNTFSTPLKGDYQ 237
Query 258 LENISTAVAAL--LVSGIDVSQAAITSGIEKAHLAGRFEQRIIKNKA-VVFDAGHNGHGV 314
+N++ AV AL L S + + +G++ GR + R + + ++FD HN
Sbjct 238 GQNLACAVTALDILSSSFSIMLPHVQNGVKNTSWPGRLDIRSVPSLGDILFDGAHNKEAA 297
Query 315 DFLLKQLRN-FLEYNKQYTEVIAVFSMLADKDIKAVVNLLKP------AVLHWKVAALSV 367
L K + + E+NK + V+A + KD+ ++ +L A V+ +
Sbjct 298 IELAKFVNSQRREHNKSVSWVVAFTN---TKDVTGIMKILLRKGDTVIATNFSSVSGMPW 354
Query 368 PRAAPVTQLLDALDGE-----ITQQYDSIREAFSVALEESRPHQLILVCGSFHTLEAVWE 422
++ + +++ E T +I E +A E+ + ++VCGS + L ++
Sbjct 355 IKSMEPEVIKNSISSESSVECYTADNLTISEILRLAKEK---NSSVIVCGSLYLLGDMYR 411
Query 423 YLE 425
YL+
Sbjct 412 YLK 414
>Q9HS44 Probable folylpolyglutamate synthase / Probable dihydropteroate
synthase [Halobacterium salinarum (strain ATCC 700922
/ JCM 11081 / NRC-1)]
Length=815
Score = 144 bits (363), Expect = 2e-40
Identities = 122/377 (32%), Positives = 173/377 (46%), Gaps = 26/377 (7%)
Query 53 VAGTNGKGSTTTTLASILTEQGYRVGLYQSPHIYRFNERVKLNGQEASDQQL---IEAFV 109
+AG+NGKGST SIL G VGLY SPH ERV +NG+ S L IE
Sbjct 45 IAGSNGKGSTARFTESILRAAGLDVGLYTSPHFDDVRERVTVNGRRMSKAALTTFIETIT 104
Query 110 KVDQVRRECGLSLSFFEATTLAAFLIFQQQACDIWVLEVGLGGRLDVVNVIDPDVAVITN 169
R G + ++FE T A F + D+ VLEVG+GGRLD +V+DP + +T+
Sbjct 105 PYVTERAADGAAPTYFEVVTAMALWQFSRADVDVAVLEVGIGGRLDATSVVDPSASAVTS 164
Query 170 IGLDHTDWLGDTIEKIAFEKAGII-RSNIPVIFGGVQTLPQAIADKAAAQQAQLYVLGRD 228
+ L+HTD LG+TI +IA +KA + + P++ A+ D A A + GRD
Sbjct 165 VTLEHTDVLGETIPEIARDKAHVAPAGDTPLVTATTDDALAAVRDHAGAVRTVGDTAGRD 224
Query 229 Y--FYQVAPDQKSWNFASSG----TTLKLP-LGSLALENISTAVAALLVSGIDVSQAAIT 281
Y+ + + +G ++P LG EN A AAL V AI
Sbjct 225 VTATYEGRTNHTEAAVSITGDDWSVATEIPLLGDHQAENAGVA-AALARQTAGVDDDAIA 283
Query 282 SGIEKAHLAGRFEQRIIKNKAVVFDAGHNGHGVDFLLKQLRNFLEYNKQYTEVIAVFSML 341
G+ AH GRFE + + VV D HN + + + F Y ++ VF +
Sbjct 284 RGLRSAHWPGRFEV-MGADPVVVLDGAHNPGACGAVAETVAEF-----DYDRLLTVFGAM 337
Query 342 ADKDIKAVVNLLKPAVLHWKVAALSVPRAAPVTQLL-----DALDGEITQQYDSIREAFS 396
DKD A+ L W A VP A +L DA G ++ ++ A +
Sbjct 338 HDKDHGAMAAALPTPDHVW--ACEPVPDRAEDADVLAAVFEDAGAGTVSVT-RAVESAVA 394
Query 397 VALEESRPHQLILVCGS 413
A+ E+ L+LV GS
Sbjct 395 DAIAEATADDLVLVAGS 411
Score = 18.5 bits (36), Expect = 7.7
Identities = 6/11 (55%), Positives = 10/11 (91%), Gaps = 0/11 (0%)
Query 31 LERVIPVAEKL 41
L+RV+PV E++
Sbjct 613 LDRVLPVIERI 623
>D4GW05 Probable folylpolyglutamate synthase / Probable dihydropteroate
synthase [Haloferax volcanii (strain ATCC 29605 /
DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 /
DS2)]
Length=838
Score = 143 bits (360), Expect = 4e-40
Identities = 120/408 (29%), Positives = 181/408 (44%), Gaps = 31/408 (8%)
Query 30 GLERVIPVAEKLGVTQTSAKVFTVAGTNGKGSTTTTLASILTEQGYRVGLYQSPHIYRFN 89
G E + + LG VAG+NGKGST + ++L E G VGLY SPH
Sbjct 21 GTESIRRLLSHLGDPHEGVSFVQVAGSNGKGSTARMVDAMLRESGAHVGLYTSPHFDDVR 80
Query 90 ERVKLNGQEASDQQLIEAFV---KVDQVRREC-GLSLSFFEATTLAAFLIFQQQACDIWV 145
ERV+++G++ L AFV K V R G L+FFE T A F + D+ V
Sbjct 81 ERVRVDGRKIPKSAL-SAFVAEAKPYLVERAADGEPLTFFETVTALALWYFDRAGVDVAV 139
Query 146 LEVGLGGRLDVVNVIDPDVAVITNIGLDHTDWLGDTIEKIAFEKAGIIRSNIPVIFGGVQ 205
LEVG+GG LD + +DP + +TN+ L+HT LGDT+ +IA KA + ++ P++ G
Sbjct 140 LEVGMGGELDATSAVDPVASAVTNVSLEHTAVLGDTVAEIAKTKAAVAPADAPLVTGTTG 199
Query 206 TLPQAIADKA----------AAQQAQLYVLGRDYFYQVAPDQKSWNFASSGTTL--KLP- 252
I ++A A A + V Y +V + + + TL ++P
Sbjct 200 EALSVIREEAGDVLTVGDADADSDADVRV---SYGGRVNHQEAAVTVETDAETLDVRIPL 256
Query 253 LGSLALENISTAVAALLVSGIDVSQAAITSGIEKAHLAGRFEQRIIKNKAVVFDAGHNGH 312
LG+ N AV+ D+ AI G+ AH GRFE + VV D HN
Sbjct 257 LGAYQARNAGIAVSLARQVRPDIDAEAIHRGLRNAHWPGRFEV-MGTEPTVVLDGAHNPD 315
Query 313 GVDFLLKQLRNFLEYNKQYTEVIAVFSMLADKDIKAVVNLLKPAVLHWKVAALSVPRAAP 372
+ L F Y ++ V+ + DKD +V L P V + R
Sbjct 316 ACAQVATVLDEF-----DYDDLHLVYGAMHDKDHGEMVGAL-PEVASVVTCKADISRGED 369
Query 373 ---VTQLLDALDGEITQQYDSIREAFSVALEESRPHQLILVCGSFHTL 417
++ + + L+G + ++ A A + P +LV GS + +
Sbjct 370 PEILSSVFERLNGPAVETGGAVAAALDRARARADPDDCVLVVGSLYVV 417
>Q12676 Dihydrofolate synthetase [Saccharomyces cerevisiae (strain
ATCC 204508 / S288c)]
Length=427
Score = 139 bits (350), Expect = 4e-40
Identities = 104/330 (32%), Positives = 166/330 (50%), Gaps = 25/330 (8%)
Query 27 IDLGLERVIPVAEKLGVTQTSAKVFTVAGTNGKGSTTTTLASILTEQGYRVGLYQSPHIY 86
I+LGL R+ + E LG Q S +V +AGTNGKGS T L+S+L ++ Y++G + +PH+
Sbjct 3 IELGLSRITKLLEHLGNPQNSLRVLHIAGTNGKGSVCTYLSSVLQQKSYQIGKFTTPHLV 62
Query 87 RFNERVKLNGQEASDQQLIEAFVKVDQVRRECGLSLSFFEATTLAAFLIFQQQACDIWVL 146
+ + +N + ++ ++++ + + L + FE T AF F C V+
Sbjct 63 HVTDSITINNKPIPLERYQNIRLQLEALNKSHSLKCTEFELLTCTAFKYFYDVQCQWCVI 122
Query 147 EVGLGGRLDVVNVI---DPDVAVITNIGLDHTDWLGDTIEKIAFEKAGIIRSNIP--VIF 201
EVGLGGRLD NVI + IT I LDH +LG+T+ +I+ EKAGII +P VI
Sbjct 123 EVGLGGRLDATNVIPGANKACCGITKISLDHESFLGNTLSEISKEKAGIITEGVPFTVID 182
Query 202 G-GVQTLPQAIADKAAAQQAQLYVLGRDYFYQVAPDQKSWNFASSGTTLKLPL-GSLALE 259
G ++ + ++ A ++L V + D SW KLPL G +
Sbjct 183 GTNEASVINVVKERCKALGSELSVTDSQLNGNMI-DTNSWGCFD---LAKLPLNGEYQIF 238
Query 260 NISTAVAAL----LVSGIDVSQAAITSGIEKAHLAG-------RFEQRIIKNKAVVFDAG 308
N+ A+ L + ID+++ +++ + K G RF++ + ++ D
Sbjct 239 NLRVAMGMLDYLQMNELIDITKNEVSTRLAKVDWPGRLYRMDYRFDKVSNRTVPILMDGA 298
Query 309 HNGHGVDFLLKQLRNFLEY-NKQYTEVIAV 337
HNG L+K LR EY N+ T V+AV
Sbjct 299 HNGSAAVELVKYLRK--EYGNQPLTFVMAV 326
>F4JYE9 Dihydrofolate synthetase [Arabidopsis thaliana]
Length=530
Score = 125 bits (315), Expect = 1e-34
Identities = 116/377 (31%), Positives = 168/377 (45%), Gaps = 55/377 (15%)
Query 26 GIDLGLERVIPVAEKLGVTQTSAKVFTVAGTNGKGSTTTTLASILTEQGYRVGLYQSPHI 85
G DLG R+ + +L KV VAGT GKGST+ L++IL GY VG Y SPHI
Sbjct 74 GFDLG--RMKRLMLRLRNPHYKYKVVHVAGTKGKGSTSAFLSNILRAGGYSVGCYSSPHI 131
Query 86 YRFNERVKLNGQEASDQQLIEAFVKV----DQVRRECGLSLSFFEATTLAAFLIFQQQAC 141
ER+ NG+ S L + F V +Q +E SLS FE T AF +F+++
Sbjct 132 LSIKERISCNGEPVSASTLNDLFYSVKPILEQSIQEENGSLSHFEILTGIAFSLFEKENV 191
Query 142 DIWVLEVGLGGRLDVVNVIDPD---VAVITNIGLDHTDWLGDTIEKIAFEKAGIIRSNIP 198
DI V+E GLGG D NVI+ +VIT IG +H LG ++E IA K+GII+ P
Sbjct 192 DIAVIEAGLGGARDATNVIESSNLAASVITTIGEEHMAALGGSLESIAEAKSGIIKHGRP 251
Query 199 VIFGG--VQTLPQAIADKAAAQQAQLYVLGR----------------------DYFYQVA 234
V+ GG + + + KAA+ + + + D Q
Sbjct 252 VVLGGPFLPHIEGILRSKAASVSSSVILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNE 311
Query 235 PDQKSWNFASSGTTLKLPLGSLALENISTAVAALLVSGID----VSQAAITSGIEKAHLA 290
D + S L++ LG L+N TA L V+ AI G+E L
Sbjct 312 KDDQP-IVELSDVNLRM-LGHHQLQNAVTATCVSLCLRDQGCGRVTDEAIRIGLENTRLL 369
Query 291 GR--------FEQRIIKNKAVVFDAGHNGHGVDFLLKQLRNFLEYNKQYTEVIAVFSMLA 342
GR E ++ V+ D H + L+ ++ + ++ V +M +
Sbjct 370 GRSQFLTPKEAETLLLPGATVLLDGAHTKESA----RALKEMIKKDFPEKRLVFVVAMAS 425
Query 343 DKD----IKAVVNLLKP 355
DKD K +++ LKP
Sbjct 426 DKDHVSFAKELLSGLKP 442
>I6Y0R5 Dihydrofolate synthase/folylpolyglutamate synthase [Mycobacterium
tuberculosis (strain ATCC 25618 / H37Rv)]
Length=487
Score = 125 bits (313), Expect = 1e-34
Identities = 120/412 (29%), Positives = 185/412 (45%), Gaps = 47/412 (11%)
Query 25 TGIDLGLERVIPVAEKLGVTQTSAKVFTVAGTNGKGSTTTTLASILTEQGYRVGLYQSPH 84
T ID L R+ + + LG Q S +AGTNGK S + +++T R G SPH
Sbjct 41 TRIDPSLTRISALMDLLGSPQRSYPSIHIAGTNGKTSVARMVDALVTALHRRTGRTTSPH 100
Query 85 IYRFNERVKLNGQEASDQQLIEAFVKVDQV-----------RRECGLSLSFFEATTLAAF 133
+ ER+ ++G+ S Q + + +++ + + G ++S FE T AF
Sbjct 101 LQSPVERISIDGKPISPAQYVATYREIEPLVALIDQQSQASAGKGGPAMSKFEVLTAMAF 160
Query 134 LIFQQQACDIWVLEVGLGGRLDVVNVIDPDVAVITNIGLDHTDWLGDTIEKIAFEKAGII 193
F D+ V+EVG+GGR D NVI+ VAVIT I +DH D+LG I IA EKAGII
Sbjct 161 AAFADAPVDVAVVEVGMGGRWDATNVINAPVAVITPISIDHVDYLGADIAGIAGEKAGII 220
Query 194 R-------SNIPVIFGGVQTLPQAI------ADKAAAQQ-AQLYVLGRDYFYQVAPDQKS 239
+ VI V + + + AD + A++ ++ VL R Q+A +
Sbjct 221 TRAPDGSPDTVAVIGRQVPKVMEVLLAESVRADASVAREDSEFAVLRR----QIAVGGQV 276
Query 240 WNFASSG---TTLKLPL-GSLALENISTAVA---ALLVSGI--DVSQAAITSGIEKAHLA 290
G + + LPL G N A+A A +G + A+ +G
Sbjct 277 LQLQGLGGVYSDIYLPLHGEHQAHNAVLALASVEAFFGAGAQRQLDGDAVRAGFAAVTSP 336
Query 291 GRFEQRIIKNKAVVFDAGHNGHGVDFLLKQLRNFLEYNKQYTEVIAVFSMLADKDIKAVV 350
GR E R+ V DA HN G L + L + ++ ++ V S+L DKD+ ++
Sbjct 337 GRLE-RMRSAPTVFIDAAHNPAGASALAQTLAHEFDFR----FLVGVLSVLGDKDVDGIL 391
Query 351 NLLKPAVLHWKVAALSVPRAAPVTQLL----DALDGEITQQYDSIREAFSVA 398
L+P V PRA V L + + + +++R+A VA
Sbjct 392 AALEPVFDSVVVTHNGSPRALDVEALALAAGERFGPDRVRTAENLRDAIDVA 443
>Q8W035 Folylpolyglutamate synthase [Arabidopsis thaliana]
Length=492
Score = 122 bits (307), Expect = 9e-34
Identities = 105/351 (30%), Positives = 165/351 (47%), Gaps = 32/351 (9%)
Query 49 KVFTVAGTNGKGSTTTTLASILTEQGYRVGLYQSPHIYRFNERVKLNGQEASDQQLIE-- 106
K+ VAGT GKGST T SIL G R GL+ SPH+ ER +LNG E S ++ +
Sbjct 59 KIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYF 118
Query 107 --AFVKVDQVRRECGLSLSFFEATTLAAFLIFQQQACDIWVLEVGLGGRLDVVNVID-PD 163
F K+ + ++F L AF IF + D+ +LEVGLGGR D NVI P
Sbjct 119 WCCFHKLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPV 178
Query 164 VAVITNIGLDHTDWLGDTIEKIAFEKAGIIRSNIPVI-FGGVQTLPQAIADKAAAQQAQL 222
V I+++G DH + LG T+ +IA EKAGI +S +P + + +KA+ + L
Sbjct 179 VCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNL 238
Query 223 YVLGRDYFYQVAPDQKSWNFASSGTTLKLPLGSLALENISTAVAALLV---SGIDVSQA- 278
V V P S G L G LA+ ST + + + +G+D +
Sbjct 239 QV--------VEPLDSSQRLGLQGEHQYLNAG-LAVALCSTFLKEIGIEDKNGLDQTNGL 289
Query 279 --AITSGIEKAHLAGRF----EQRIIKNKAVVFDAGHNGHGVD----FLLKQLRNFLEYN 328
SG+ A+L GR + + + D H+ ++ + KQ++ E N
Sbjct 290 PEKFISGLSNAYLMGRAMIVPDSELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQERN 349
Query 329 KQYTEVIAVFSMLADKDIKAVVNLLKPAVLHWKV---AALSVPRAAPVTQL 376
++ +E I +F+ ++ +D ++ L+ + V A+ VP + Q+
Sbjct 350 QKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQV 400
Score = 21.9 bits (45), Expect = 0.62
Identities = 13/47 (28%), Positives = 24/47 (51%), Gaps = 2/47 (4%)
Query 8 IQDDLMTWLTYWGHVHVTGIDLGLERVIPVAEKLGVTQTSAKVFTVA 54
IQ ++ ++ G+ H+ + L + AEK G+ ++ FTVA
Sbjct 174 IQKPVVCGISSLGYDHMEILGYTLAEI--AAEKAGIFKSGVPAFTVA 218
>F4J2K2 Folylpolyglutamate synthase [Arabidopsis thaliana]
Length=625
Score = 115 bits (288), Expect = 6e-31
Identities = 68/166 (41%), Positives = 101/166 (61%), Gaps = 10/166 (6%)
Query 49 KVFTVAGTNGKGSTTTTLASILTEQGYRVGLYQSPHIYRFNERVKLNGQEASDQQLIEAF 108
KV VAGT GKGST +IL G+R G++ SPH+ ER +++G + S+++ ++ F
Sbjct 132 KVIHVAGTKGKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYF 191
Query 109 VKVDQVRRE---CGLSL-SFFEATTLAAFLIFQQQACDIWVLEVGLGGRLDVVNVID-PD 163
+ ++ +E GL++ F+ T+ AF IF + D+ V+EVGLGG+LD NVI P
Sbjct 192 WECWKLLKEKAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPV 251
Query 164 VAVITNIGLDHTDWLGDTIEKIAFEKAGIIRSNIPVIFGGVQTLPQ 209
V I ++G+DH D LG+T+ IAF KAGI + IP T+PQ
Sbjct 252 VCGIASLGMDHMDILGNTLADIAFHKAGIFKPQIPAF-----TVPQ 292
Score = 20.8 bits (42), Expect = 1.5
Identities = 18/61 (30%), Positives = 27/61 (44%), Gaps = 9/61 (15%)
Query 368 PRAAPVTQ--LLDALDGEITQQYDSIREAFSVALEESRPHQLILVCGSFHTLEAVWEYLE 425
P PVT ++ +L I D +R S+ LE +LV GS H + V L+
Sbjct 572 PAGTPVTSSAVMPSLPLTINWLRDCVRRNPSLKLE-------VLVTGSLHLVGDVLRLLK 624
Query 426 K 426
+
Sbjct 625 R 625
Score = 20.0 bits (40), Expect = 2.5
Identities = 13/48 (27%), Positives = 16/48 (33%), Gaps = 0/48 (0%)
Query 378 DALDGEITQQYDSIREAFSVALEESRPHQLILVCGSFHTLEAVWEYLE 425
D D YD EA S + G+ LE V YL+
Sbjct 73 DKADNPALSSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLK 120
Lambda K H a alpha
0.322 0.136 0.399 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 2572271
Database: 7b4a06566d328942ef1243b1e36c9acb.SwissProt.fasta
Posted date: May 9, 2024 7:51 PM
Number of letters in database: 7,603
Number of sequences in database: 15
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
