BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 6b9fee4299a35919551e381782b22509.SwissProt.fasta
10 sequences; 2,952 total letters
Query= ACIAD0765
Length=264
Score E
Sequences producing significant alignments: (Bits) Value
O80475 Methylesterase 8 [Arabidopsis thaliana] 91.3 2e-25
Q8S8S9 Methylesterase 1 [Arabidopsis thaliana] 89.4 1e-24
O80476 Methylesterase 2 [Arabidopsis thaliana] 87.8 4e-24
C0LA90 Pyrethroid hydrolase [Sphingobium wenxiniae (strain DSM 21... 83.2 2e-22
O80477 Methylesterase 3 [Arabidopsis thaliana] 79.3 5e-21
Q9FVW3 Putative methylesterase 14, chloroplastic [Arabidopsis tha... 80.1 6e-21
Q6RYA0 Salicylic acid-binding protein 2 [Nicotiana tabacum] 77.4 2e-20
Q9SE93 Polyneuridine-aldehyde esterase [Rauvolfia serpentina] 75.9 8e-20
Q9FW03 Putative methylesterase 11, chloroplastic [Arabidopsis tha... 76.3 2e-19
Q940H7 Putative methylesterase 12, chloroplastic [Arabidopsis tha... 75.9 2e-19
>O80475 Methylesterase 8 [Arabidopsis thaliana]
Length=272
Score = 91.3 bits (225), Expect = 2e-25
Identities = 79/251 (31%), Positives = 122/251 (49%), Gaps = 19/251 (8%)
Query 1 MAKHIVLVHGAWQGSWSFDLIKPWLQQKGWTVHAVDLPDNG--WNSDTQITASQQSYCDY 58
M +H VLVHG+ G+W + +KP L+ G V A+DL G S T I+ +Q Y +
Sbjct 24 MKQHFVLVHGSCLGAWCWYKVKPLLEASGHRVTALDLAACGIDTRSITDISTCEQ-YSEP 82
Query 59 VVQMIHNI--GEPVVLLGHSGGGLTISAVAEQIPELISHLVYLVGMMLPS-NMSFLDFKK 115
++Q++ ++ E VVL+GHS GGLT++ ++ P+ IS V++ M + N +K
Sbjct 83 LIQLMTSLPNDEKVVLVGHSYGGLTLAIAMDKFPDKISVSVFVTSFMPDTKNSPSFVLEK 142
Query 116 LCEQHFPDEDFSG--ISPYLTFTDDGYSIVSSEGAKKIFLQDCEPALAEQLIEKLRPQPE 173
P ED+ G + PY+ F S+E K LQ E + RP
Sbjct 143 FASTMTP-EDWMGSELEPYVVF--------SAEFTKHRILQLSPIEDLELRLLLKRPGSL 193
Query 174 AGRDLKPV--LTPERFGCVPRIYVEALNDQSLSINMQRLMQQLQPNKLQIIAMQTGHVPQ 231
DL + + + +G VPR Y+ + +D ++S QR M P L I T H+P
Sbjct 194 FLNDLSRMKNFSEKGYGSVPRAYIVSKDDHTISEEYQRWMIDNYPPNLVIEMEGTDHLPL 253
Query 232 AVQPDLLVEKL 242
+P LL + L
Sbjct 254 FCKPQLLSDHL 264
>Q8S8S9 Methylesterase 1 [Arabidopsis thaliana]
Length=263
Score = 89.4 bits (220), Expect = 1e-24
Identities = 72/245 (29%), Positives = 118/245 (48%), Gaps = 10/245 (4%)
Query 3 KHIVLVHGAWQGSWSFDLIKPWLQQKGWTVHAVDLPDNGWNSDTQIT--ASQQSYCDYVV 60
+H VLVHG+ G+W + +KP L+ G V AVDL +G ++ IT + + Y + +
Sbjct 8 QHFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYSEPLT 67
Query 61 QMIHNI--GEPVVLLGHSGGGLTISAVAEQIPELISHLVYLVGMMLPSNMSFLDFKKLCE 118
+++ ++ E VVL+GHS GGL ++ E+ PE IS V+L M + S
Sbjct 68 KLLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVLDKFG 127
Query 119 QHFPDEDFSG--ISPYLTFTDDGYSIVSSEGAKKIFLQDCEPALAEQL-IEKLRPQPEAG 175
+ P E + G PY + + G S+ S K+ L P +L + +RP
Sbjct 128 SNMPQEAWMGTEFEPYGS-DNSGLSMFFSPDFMKLGLYQLSPVEDLELGLLLMRPGSLFI 186
Query 176 RDLKPV--LTPERFGCVPRIYVEALNDQSLSINMQRLMQQLQPNKLQIIAMQTGHVPQAV 233
DL + + E +G VPR+++ D+++ QR M P L + +T H+P
Sbjct 187 NDLSKMKNFSDEGYGSVPRVFIVCKEDKAIPEERQRWMIDNFPVNLVMEMEETDHMPMFC 246
Query 234 QPDLL 238
+P L
Sbjct 247 KPQQL 251
>O80476 Methylesterase 2 [Arabidopsis thaliana]
Length=263
Score = 87.8 bits (216), Expect = 4e-24
Identities = 73/249 (29%), Positives = 121/249 (49%), Gaps = 10/249 (4%)
Query 3 KHIVLVHGAWQGSWSFDLIKPWLQQKGWTVHAVDLPDNGWNSDTQIT--ASQQSYCDYVV 60
+H VLVHGA G+W + +KP L+ G V A+DL +G ++ IT ++ + Y + ++
Sbjct 8 QHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEPLM 67
Query 61 QMIHNI--GEPVVLLGHSGGGLTISAVAEQIPELISHLVYLVGMMLPSNMSFLDFKKLCE 118
Q++ ++ E VVL+GHS GGL+++ ++ P+ IS V++ M + S ++
Sbjct 68 QLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEKFA 127
Query 119 QHFPDEDFSGISPYLTFTDD--GYSIVSSEGAKKIFLQDCEPALAEQL-IEKLRPQPEAG 175
E + G S T+ D G S+ S K L P +L + RP
Sbjct 128 SSMTPEGWMG-SELETYGSDNSGLSVFFSTDFMKHRLYQLSPVEDLELGLLLKRPSSLFI 186
Query 176 RDLKPV--LTPERFGCVPRIYVEALNDQSLSINMQRLMQQLQPNKLQIIAMQTGHVPQAV 233
+L + + + +G VPR Y+ D +S + QR M P L I +T H+P
Sbjct 187 NELSKMENFSEKGYGSVPRAYIVCKEDNIISEDHQRWMIHNYPANLVIEMEETDHMPMFC 246
Query 234 QPDLLVEKL 242
+P LL + L
Sbjct 247 KPQLLSDHL 255
>C0LA90 Pyrethroid hydrolase [Sphingobium wenxiniae (strain DSM
21828 / JZ-1)]
Length=280
Score = 83.2 bits (204), Expect = 2e-22
Identities = 67/255 (26%), Positives = 115/255 (45%), Gaps = 33/255 (13%)
Query 5 IVLVHGAWQGSWSFDLIKPWLQQKGWTVHAVDLPDNGWNSDTQITA-SQQSYCDYVVQMI 63
I+L+HGA +D + P L+ +G+ VHA DL + ++ + Y V ++
Sbjct 6 IILIHGALNRGACYDAVVPLLEARGYRVHAPDLTGHTPGDGGHLSVVDMEHYTRPVADIL 65
Query 64 HNIGEPVVLLGHSGGGLTISAVAEQIPELISHLVYLVGMMLPSNMSFLDFKKLCEQHFPD 123
+LLGHS GG +IS +A+ P+ ++ L+YL ++ ++ F P
Sbjct 66 ARAEGQSILLGHSLGGASISWLAQHHPDKVAGLIYLTAVLTAPGITPETFV------LPG 119
Query 124 EDFSGISPYLTF---TDDGYSIVSS----EGAKKIFLQDCEPA---LAEQLIEKLR---- 169
E G L D+G + + E +++F+ D AEQ I+
Sbjct 120 EPNRGTPHALDLIQPVDEGRGLQADFSRLERLREVFMGDYPGEGMPPAEQFIQTQSTVPF 179
Query 170 --PQPEAGRDLKPVLTPERFGCVPRIYVEALNDQSLSINMQRLMQQLQPNKLQIIAMQTG 227
P P GR L+ +PR+Y+EAL+D + I +QR MQ+ P + ++++
Sbjct 180 GTPNPMEGRALE----------IPRLYIEALDDVVIPIAVQRQMQKEFPGPVAVVSLPAS 229
Query 228 HVPQAVQPDLLVEKL 242
H P P+ L E +
Sbjct 230 HAPYYSMPERLAEAI 244
>O80477 Methylesterase 3 [Arabidopsis thaliana]
Length=263
Score = 79.3 bits (194), Expect = 5e-21
Identities = 74/259 (29%), Positives = 122/259 (47%), Gaps = 24/259 (9%)
Query 3 KHIVLVHGAWQGSWSFDLIKPWLQQKGWTVHAVDLPDNGWNSDTQIT--ASQQSYCDYVV 60
+H+VLVHGA G+W + +KP L+ G V AVDL +G + IT ++ + Y + ++
Sbjct 8 QHVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQYSEPLM 67
Query 61 QMIHNI--GEPVVLLGHSGGGLTISAVAEQIPELISHLVYLVGMMLPSNMSFLDF----- 113
Q++ ++ E VVL+GHS GGL+++ + P IS V++ MM P F
Sbjct 68 QLMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMM-PDTKHSPSFVWDKL 126
Query 114 -KKLCEQHFPDEDFSGISPYLTFTDDGYSIVSSEGAKKIF----LQDCEPALAEQLIEKL 168
K+ + + D F+ P F + + AK ++ +QD E LA+ L+
Sbjct 127 RKETSREEWLDTVFTSEKP--DFPSEFWIFGPEFMAKNLYQLSPVQDLE--LAKMLV--- 179
Query 169 RPQPEAGRDL--KPVLTPERFGCVPRIYVEALNDQSLSINMQRLMQQLQPNKLQIIAMQT 226
R P +D+ + + E +G V RI++ D + QR M P K +
Sbjct 180 RANPLIKKDMAERRSFSEEGYGSVTRIFIVCGKDLVSPEDYQRSMISNFPPKEVMEIKDA 239
Query 227 GHVPQAVQPDLLVEKLNQI 245
H+P +P L L +I
Sbjct 240 DHMPMFSKPQQLCALLLEI 258
>Q9FVW3 Putative methylesterase 14, chloroplastic [Arabidopsis
thaliana]
Length=348
Score = 80.1 bits (196), Expect = 6e-21
Identities = 72/255 (28%), Positives = 121/255 (47%), Gaps = 14/255 (5%)
Query 1 MAKHIVLVHGAWQGSWSFDLIKPWLQQKGWTVHAVDLPDNGWN-SDTQITASQQSYCDYV 59
+ K VLVHG G+W + + L++ G + VDL G+N +DT ++ + Y +
Sbjct 94 IVKKFVLVHGEGFGAWCWYKMVASLEESGLSPVTVDLTGCGFNMTDTNTVSTLEEYSKPL 153
Query 60 VQMIHNIGEP--VVLLGHSGGGLTISAVAEQIPELISHLVYLVGMMLPSNMSFLDFKKLC 117
+ ++ N+ E V+L+GHS GG +IS E+ PE IS +++ M+ F
Sbjct 154 IDLLENLPEEEKVILVGHSTGGASISYALERFPEKISKAIFVCATMVSDGQR--PFDVFS 211
Query 118 EQHFPDEDFSGISPYLTFTD------DGYSIVSSEGAKKIFLQDCEPALAEQLIEKLRPQ 171
E+ E F S +L + + G+ F Q +A +I +RP
Sbjct 212 EELGSAERFMKESQFLIYGNGKDKPPTGFMFEKPHMKGLYFNQSPNKDIALAMI-SMRPV 270
Query 172 PEAGRDLKPVLTPERFGCVPRIYVEALNDQSLSINMQ-RLMQQLQPNKLQIIAMQTGHVP 230
P K LT ER+G R YV+ L+D++LS ++Q +L+++ P + I + H P
Sbjct 271 PLGPMMEKVSLTAERYGKGRRFYVQTLDDRALSPDVQEKLVRENSPEGVFKI-KGSDHCP 329
Query 231 QAVQPDLLVEKLNQI 245
+P L + L +I
Sbjct 330 FFSKPQSLHKILLEI 344
>Q6RYA0 Salicylic acid-binding protein 2 [Nicotiana tabacum]
Length=260
Score = 77.4 bits (189), Expect = 2e-20
Identities = 68/223 (30%), Positives = 102/223 (46%), Gaps = 24/223 (11%)
Query 3 KHIVLVHGAWQGSWSFDLIKPWLQQKGWTVHAVDLPDNGWNSDTQITASQQSYCDYVVQM 62
KH VLVHGA G WS+ +KP L+ G V A+DL +G +D + ++ DY + +
Sbjct 5 KHFVLVHGACHGGWSWYKLKPLLEAAGHKVTALDLAASG--TDLRKIEELRTLYDYTLPL 62
Query 63 IH-----NIGEPVVLLGHSGGGLTISAVAEQIPELISHLVYLVGMMLPS--NMSFLDFKK 115
+ + E V+L+GHS GG+ + E+ P+ I V+L M S N SF+ +
Sbjct 63 MELMESLSADEKVILVGHSLGGMNLGLAMEKYPQKIYAAVFLAAFMPDSVHNSSFV--LE 120
Query 116 LCEQHFPDEDFSGISPYLTFTDDGYSIVSSEGAKKI----FLQDCEP---ALAEQLIEKL 168
+ P E++ + +L + + S K Q C P ALA L+
Sbjct 121 QYNERTPAENWLD-TQFLPYGSPEEPLTSMFFGPKFLAHKLYQLCSPEDLALASSLV--- 176
Query 169 RPQPEAGRDLKPV--LTPERFGCVPRIYVEALNDQSLSINMQR 209
RP DL T ERFG V R+Y+ D+ + QR
Sbjct 177 RPSSLFMEDLSKAKYFTDERFGSVKRVYIVCTEDKGIPEEFQR 219
>Q9SE93 Polyneuridine-aldehyde esterase [Rauvolfia serpentina]
Length=264
Score = 75.9 bits (185), Expect = 8e-20
Identities = 75/243 (31%), Positives = 115/243 (47%), Gaps = 12/243 (5%)
Query 3 KHIVLVHGAWQGSWSFDLIKPWLQQKGWTVHAVDLPDNGWN-SDTQITASQQSYCDYVVQ 61
KH VLVHG G+W + +KP L+ G V AVDL G N + + Y + +++
Sbjct 11 KHFVLVHGGCLGAWIWYKLKPLLESAGHKVTAVDLSAAGINPRRLDEIHTFRDYSEPLME 70
Query 62 MIHNI--GEPVVLLGHSGGGLTISAVAEQIPELISHLVYLVGMMLPSNMSF-LDFKKLCE 118
++ +I E VVLLGHS GG+++ E PE IS V++ MM N S F+K E
Sbjct 71 VMASIPPDEKVVLLGHSFGGMSLGLAMETYPEKISVAVFMSAMMPDPNHSLTYPFEKYNE 130
Query 119 QHFPDEDF-SGISPYLTFTDDGYSIVSSEG--AKKIFLQDCEPALAEQLIEKLRPQPEAG 175
+ D S S Y + G S++ A K+F Q+C E RP
Sbjct 131 KCPADMMLDSQFSTYGNPENPGMSMILGPQFMALKMF-QNCSVEDLELAKMLTRPGSLFF 189
Query 176 RDLKPV--LTPERFGCVPRIYVEALNDQSLSINMQR-LMQQLQPNKLQIIAMQTGHVPQA 232
+DL + ER+G V R Y+ D+S + Q+ ++ + +K++ I + H+
Sbjct 190 QDLAKAKKFSTERYGSVKRAYIFCNEDKSFPVEFQKWFVESVGADKVKEI-KEADHMGML 248
Query 233 VQP 235
QP
Sbjct 249 SQP 251
>Q9FW03 Putative methylesterase 11, chloroplastic [Arabidopsis
thaliana]
Length=390
Score = 76.3 bits (186), Expect = 2e-19
Identities = 72/253 (28%), Positives = 106/253 (42%), Gaps = 17/253 (7%)
Query 4 HIVLVHGAWQGSWSFDLIKPWLQQKGWTVHAVDLPDNGWNS-DTQITASQQSYCDYVVQM 62
H VLVHG G+W + L++ G+ V A+DL G NS + AS Y + +
Sbjct 138 HFVLVHGGSFGAWCWYKTIALLEEDGFKVTAIDLAGCGINSININGIASLSQYVKPLTDI 197
Query 63 IHN--IGEPVVLLGHSGGGLTISAVAEQIPELISHLVYLVGMMLPSNMSFLDFKKLCEQH 120
+ IGE V+L+GH GG IS E P IS V+L ML + S LD L
Sbjct 198 LEKLPIGEKVILVGHDFGGACISYAMELFPSKISKAVFLAAAMLTNGQSTLDMFSL---K 254
Query 121 FPDEDFSGISPYLTFTDDGYSIVSSEGAKKIFLQDC--------EPALAEQLIEKLRPQP 172
D + +T+ + ++ K L+D + ALA +R P
Sbjct 255 AGQNDLMRKAQIFIYTNGNENPPTAIDLDKSLLKDLLFNQSPSKDVALAS---VSMRSIP 311
Query 173 EAGRDLKPVLTPERFGCVPRIYVEALNDQSLSINMQRLMQQLQPNKLQIIAMQTGHVPQA 232
A K L+ +G V R Y+E L D ++ + +Q M P + H P
Sbjct 312 FAPVLEKLSLSDANYGSVRRYYIETLEDNAIPVTLQENMINSSPPEKVYRLKGADHAPFF 371
Query 233 VQPDLLVEKLNQI 245
+P L + L +I
Sbjct 372 SKPQALHKLLLEI 384
>Q940H7 Putative methylesterase 12, chloroplastic [Arabidopsis
thaliana]
Length=349
Score = 75.9 bits (185), Expect = 2e-19
Identities = 69/253 (27%), Positives = 118/253 (47%), Gaps = 14/253 (6%)
Query 3 KHIVLVHGAWQGSWSFDLIKPWLQQKGWTVHAVDLPDNGWN-SDTQITASQQSYCDYVVQ 61
K VLVHG G+W + L++ G + VDL +G+N +D ++ + Y +++
Sbjct 97 KKFVLVHGEGFGAWCWYKTIASLEESGLSPVTVDLAGSGFNMTDANSVSTLEEYSKPLIE 156
Query 62 MIHNIG--EPVVLLGHSGGGLTISAVAEQIPELISHLVYLVGMMLPSNMSFLDFKKLCEQ 119
+I N+ E V+L+GHS GG +S E+ PE IS +++ M+ F ++
Sbjct 157 LIQNLPAEEKVILVGHSTGGACVSYALERFPEKISKAIFICATMVTDGQR--PFDVFADE 214
Query 120 HFPDEDFSGISPYLTFTD------DGYSIVSSEGAKKIFLQDCEPALAEQLIEKLRPQPE 173
E F S +L + + G+ F Q +A +I +RP P
Sbjct 215 LGSAERFMKESQFLIYGNGKDNPATGFMFEKQHMKGLYFNQSPNKDIALSMI-SMRPVPL 273
Query 174 AGRDLKPVLTPERFGCVPRIYVEALNDQSLSINMQ-RLMQQLQPNKLQIIAMQTGHVPQA 232
K L+ ER+G R YV+ L+D +LS ++Q +L+++ P + I + H P
Sbjct 274 GPMMEKLSLSAERYGKGRRFYVQTLDDLALSPDVQEKLVRENSPEAVFKI-KGSDHCPFF 332
Query 233 VQPDLLVEKLNQI 245
+P L + L +I
Sbjct 333 SKPQSLHKILLEI 345
Lambda K H a alpha
0.321 0.137 0.418 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 566866
Database: 6b9fee4299a35919551e381782b22509.SwissProt.fasta
Posted date: May 9, 2024 8:01 AM
Number of letters in database: 2,952
Number of sequences in database: 10
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
