BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 803c12b249bcbf6f6fcd5083122f1bca.SwissProt.fasta
4 sequences; 1,837 total letters
Query= ACIAD1074
Length=447
Score E
Sequences producing significant alignments: (Bits) Value
P42308 Citrate transporter [Bacillus subtilis (strain 168)] 514 0.0
P55069 Mg(2+)/citrate complex secondary transporter [Bacillus sub... 449 4e-160
O05407 Uncharacterized transporter YraO [Bacillus subtilis (strai... 414 2e-146
Q8LG88 Tonoplast dicarboxylate transporter [Arabidopsis thaliana] 42.4 7e-08
>P42308 Citrate transporter [Bacillus subtilis (strain 168)]
Length=426
Score = 514 bits (1325), Expect = 0.0
Identities = 258/442 (58%), Positives = 337/442 (76%), Gaps = 22/442 (5%)
Query 1 MLTILGIGMIMCFMYLIMTKRLSAMVALILIPVLFALIGWGLGFHFDSLSHIEVTKLGET 60
ML ILG M++ FMYLIM+ RLSA++ALI++P++FALI GF D LGE
Sbjct 1 MLAILGFVMMIVFMYLIMSNRLSALIALIVVPIVFALIS---GFGKD---------LGEM 48
Query 61 MLDGIKKLAPTGVMLLFAIMYFALMIDSGLFDPSIKWILKKVKGDPLKITLGTVFLTLTV 120
M+ G+ LAPTG+MLLFAI+YF +MIDSGLFDP I IL VKGDPLKI +GT LT+T+
Sbjct 49 MIQGVTDLAPTGIMLLFAILYFGIMIDSGLFDPLIAKILSFVKGDPLKIAVGTAVLTMTI 108
Query 121 SLDGDGSTTYMICVAAMMPLYKRIGMNPLVMTCLMLLSSGVMNLTPWGGPTARAASALQV 180
SLDGDG+TTYMI +AAM+PLYKR+GMN LV+ + +L SGVMN+ PWGGPTAR ++L++
Sbjct 109 SLDGDGTTTYMITIAAMLPLYKRLGMNRLVLAGIAMLGSGVMNIIPWGGPTARVLASLKL 168
Query 181 DASHVFIPMIPSMVIAAAWLFVLAYMYGKYERKRLGVIEMNDVSHADNLTISKDPEA--- 237
D S VF P+IP+M+ W+ +AY+ GK ERKRLGVI + HA S DPEA
Sbjct 169 DTSEVFTPLIPAMIAGILWVIAVAYILGKKERKRLGVIS---IDHAP----SSDPEAAPL 221
Query 238 QRPHLRWFNGILTIVLMAALVKGILPMAILFMLGFCIALIVNYPDLQMQKKRIAMHADSV 297
+RP L+WFN +LT+ LMAAL+ +LP+ +LFM F +AL+VNYP+++ Q+KRI+ HA +
Sbjct 222 KRPALQWFNLLLTVALMAALITSLLPLPVLFMTAFAVALMVNYPNVKEQQKRISAHAGNA 281
Query 298 LAVVGVIFAAGVFTGILTGTGMVEAMSKGFVAIIPASMGPYMAPITGVLSMPLTFFMSND 357
L VV ++FAAG+FTGIL+GT MV+AM+ V++IP +MGP++ IT ++SMP TFFMSND
Sbjct 282 LNVVSMVFAAGIFTGILSGTKMVDAMAHSLVSLIPDAMGPHLPLITAIVSMPFTFFMSND 341
Query 358 AFYFGVLPILAEAAAQYGIEPVEIARASIVGQPIHLLSPLVPSTYLLCGLAAVEFADHQK 417
AFYFGVLPI+AEAA+ YGI+ EI RAS++GQP+HLLSPLVPSTYLL G+A V F DHQK
Sbjct 342 AFYFGVLPIIAEAASAYGIDAAEIGRASLLGQPVHLLSPLVPSTYLLVGMAGVSFGDHQK 401
Query 418 FTLKWAFITSCILMGSALVFGV 439
FT+KWA T+ ++ +AL+ G+
Sbjct 402 FTIKWAVGTTIVMTIAALLIGI 423
Score = 21.6 bits (44), Expect = 0.20
Identities = 23/103 (22%), Positives = 40/103 (39%), Gaps = 42/103 (41%)
Query 172 ARAASALQVDAS------------HVFIPMIPSMVIAAAWLFVLAYMYGKYERKRLGVIE 219
A AASA +DA+ H+ P++PS + ++
Sbjct 352 AEAASAYGIDAAEIGRASLLGQPVHLLSPLVPSTYL---------------------LVG 390
Query 220 MNDVSHADNLTISKDPEAQRPHLRWFNGILTIVLMAALVKGIL 262
M VS D+ Q+ ++W G ++ +AAL+ GI+
Sbjct 391 MAGVSFGDH---------QKFTIKWAVGTTIVMTIAALLIGII 424
Score = 17.7 bits (34), Expect = 3.2
Identities = 8/32 (25%), Positives = 17/32 (53%), Gaps = 0/32 (0%)
Query 411 EFADHQKFTLKWAFITSCILMGSALVFGVFPL 442
E A ++ L+W + + + +AL+ + PL
Sbjct 217 EAAPLKRPALQWFNLLLTVALMAALITSLLPL 248
Score = 17.3 bits (33), Expect = 4.2
Identities = 5/15 (33%), Positives = 9/15 (60%), Gaps = 0/15 (0%)
Query 430 LMGSALVFGVFPLVS 444
+ A FGV P+++
Sbjct 338 MSNDAFYFGVLPIIA 352
>P55069 Mg(2+)/citrate complex secondary transporter [Bacillus
subtilis (strain 168)]
Length=433
Score = 449 bits (1155), Expect = 4e-160
Identities = 229/442 (52%), Positives = 310/442 (70%), Gaps = 11/442 (2%)
Query 1 MLTILGIGMIMCFMYLIMTKRLSAMVALILIPVLFALIGWGLGFHFDSLSHIEVTKLGET 60
ML ILG M++ FM LIMTKRLS + AL+L P++FALI G GF T++G+
Sbjct 1 MLAILGFLMMLVFMALIMTKRLSVLTALVLTPIVFALIA-GFGF----------TEVGDM 49
Query 61 MLDGIKKLAPTGVMLLFAIMYFALMIDSGLFDPSIKWILKKVKGDPLKITLGTVFLTLTV 120
M+ GI+++APT VM++FAI+YF +MID+GLFDP + IL VKGDPLKI +GT LT+ V
Sbjct 50 MISGIQQVAPTAVMIMFAILYFGIMIDTGLFDPMVGKILSMVKGDPLKIVVGTAVLTMLV 109
Query 121 SLDGDGSTTYMICVAAMMPLYKRIGMNPLVMTCLMLLSSGVMNLTPWGGPTARAASALQV 180
+LDGDGSTTYMI +AM+PLY +G+ P+++ + + G+MN PWGG T RAASAL V
Sbjct 110 ALDGDGSTTYMITTSAMLPLYLLLGIRPIILAGIAGVGMGIMNTIPWGGATPRAASALGV 169
Query 181 DASHVFIPMIPSMVIAAAWLFVLAYMYGKYERKRLGVIEMNDVSHADNLTISKDPEAQRP 240
D + + PMIP + + +AY+ GK ERKRLGVIE+ ++A+ + + E +RP
Sbjct 170 DPAELTGPMIPVIASGMLCMVAVAYVLGKAERKRLGVIELKQPANANEPAAAVEDEWKRP 229
Query 241 HLRWFNGILTIVLMAALVKGILPMAILFMLGFCIALIVNYPDLQMQKKRIAMHADSVLAV 300
L WFN +LT+ L+ LV G + + +LF++ FCIALIVNYP+L+ Q++RIA H+ +VLA+
Sbjct 230 KLWWFNLLLTLSLIGCLVSGKVSLTVLFVIAFCIALIVNYPNLEHQRQRIAAHSSNVLAI 289
Query 301 VGVIFAAGVFTGILTGTGMVEAMSKGFVAIIPASMGPYMAPITGVLSMPLTFFMSNDAFY 360
+IFAAGVFTGILTGT MV+ M+ V++IP MG + I + S TF M NDA++
Sbjct 290 GSMIFAAGVFTGILTGTKMVDEMAISLVSMIPEQMGGLIPAIVALTSGIFTFLMPNDAYF 349
Query 361 FGVLPILAEAAAQYGIEPVEIARASIVGQPIHLLSPLVPSTYLLCGLAAVEFADHQKFTL 420
+GVLPIL+E A YG++ VEIARASI+GQPIH+LSPLVPST+LL GL V DHQKF L
Sbjct 350 YGVLPILSETAVAYGVDKVEIARASIIGQPIHMLSPLVPSTHLLVGLVGVSIDDHQKFAL 409
Query 421 KWAFITSCILMGSALVFGVFPL 442
KWA + ++ AL+ G +
Sbjct 410 KWAVLAVIVMTAIALLIGAISI 431
>O05407 Uncharacterized transporter YraO [Bacillus subtilis (strain
168)]
Length=438
Score = 414 bits (1065), Expect = 2e-146
Identities = 209/450 (46%), Positives = 312/450 (69%), Gaps = 19/450 (4%)
Query 1 MLTILGIGMIMCFMYLIMTKRLSAMVALILIPVLFALIGWGLGFHFDSLSHIEVTKLGET 60
MLTILG M+ F LIMTK++S +VAL + P++FALIG G G +G+
Sbjct 1 MLTILGFSMVTVFTILIMTKKVSPIVALTITPIVFALIG-GFG-----------KGIGDM 48
Query 61 MLDGIKKLAPTGVMLLFAIMYFALMIDSGLFDPSIKWILKKVKGDPLKITLGTVFLTLTV 120
+L+GI+ +A + +LLFAI++F ++ID+GLFDP I+ IL VKGDP+KI +G+ L + +
Sbjct 49 ILEGIQTVASSAALLLFAILFFGILIDAGLFDPLIEKILSIVKGDPVKIAIGSAVLAMLI 108
Query 121 SLDGDGSTTYMICVAAMMPLYKRIGMNPLVMTCLMLLSSGVMN-LTPWGGPTARAASALQ 179
+LDGDG+TTYMI V+AM+PLYKRIGMNP+VM L +LS +++ +TPWGGP RA S L
Sbjct 109 ALDGDGTTTYMITVSAMLPLYKRIGMNPMVMATLAMLSLSIVSGMTPWGGPATRAISVLG 168
Query 180 VDASHVFIPMIPSMVIAAAWLFVLAYMYGKYERKRLGVIEMN------DVSHADNLTISK 233
+D S F+P++P+M+ A + LA++ G+ ER R+G++++ D S + +
Sbjct 169 LDPSDFFVPLLPTMLGGIACVIFLAFLMGRKERNRIGIVQLEPRHITKDSSQSYMAATLE 228
Query 234 DPEAQRPHLRWFNGILTIVLMAALVKGILPMAILFMLGFCIALIVNYPDLQMQKKRIAMH 293
+ +RP L + N L I +M +V G ++LF++GF +AL +NYP+++MQK+RIA H
Sbjct 229 SEQLKRPRLIYLNLFLVISIMVFIVLGTKHPSVLFLIGFVLALTINYPNVKMQKERIAEH 288
Query 294 ADSVLAVVGVIFAAGVFTGILTGTGMVEAMSKGFVAIIPASMGPYMAPITGVLSMPLTFF 353
+ + + VV ++F+AGVF GIL+GT MV+A++ ++IIP+SMG + I + S+P TF
Sbjct 289 SGNAITVVLLVFSAGVFAGILSGTKMVDAIAGSLISIIPSSMGGFFPVIVALTSIPFTFV 348
Query 354 MSNDAFYFGVLPILAEAAAQYGIEPVEIARASIVGQPIHLLSPLVPSTYLLCGLAAVEFA 413
+SNDA+YFG++PI AEAA+ YGIEPVEIARASI+GQP+HL+SPLV ST LL + ++
Sbjct 349 LSNDAYYFGMVPIFAEAASAYGIEPVEIARASIMGQPVHLMSPLVASTVLLVSMLKMDLG 408
Query 414 DHQKFTLKWAFITSCILMGSALVFGVFPLV 443
Q+F +KWA ITS ++ A++ G ++
Sbjct 409 SFQRFAVKWAVITSLVITLLAIITGAITIL 438
>Q8LG88 Tonoplast dicarboxylate transporter [Arabidopsis thaliana]
Length=540
Score = 42.4 bits (98), Expect = 7e-08
Identities = 41/153 (27%), Positives = 68/153 (44%), Gaps = 9/153 (6%)
Query 296 SVLAVVGVIFAAGVFTGILTGTGMVEAMSKGFVAIIPASMGPY--MAPITGVLSMPLTFF 353
+++ ++G FA + G+ T +G+ E +SKG V + A PY +AP +++ +T F
Sbjct 391 NIVLLLGAGFA--IADGVRT-SGLAEVLSKGLVFLETA---PYWAIAPTVCLIAATITEF 444
Query 354 MSNDAFYFGVLPILAEAAAQYGIEPVEIARASIVGQPIHLLSPL-VPSTYLLCGLAAVEF 412
SN+A ++P+L E A GI P+ + +G L P PS + +E
Sbjct 445 TSNNATTTLLVPLLIEIAKNMGIHPLLLMVPGAIGAQFAFLLPTGTPSNVVGFTTGHIEI 504
Query 413 ADHQKFTLKWAFITSCILMGSALVFGVFPLVSM 445
D K L + L G + SM
Sbjct 505 KDMIKTGLPLKIAGTIFLSILMPTLGAYVFASM 537
Score = 25.0 bits (53), Expect = 0.017
Identities = 36/170 (21%), Positives = 70/170 (41%), Gaps = 14/170 (8%)
Query 56 KLGETMLDGIK-KLAPTGVMLLFAIMYFALMIDSGLFDPSIKWILKK----VKGDPLKIT 110
K GE ++D K K P ++LL + I G+ + +L K ++ P
Sbjct 374 KKGEKLMDWNKCKKLPWNIVLLLGAGF---AIADGVRTSGLAEVLSKGLVFLETAPYWAI 430
Query 111 LGTVFL---TLTVSLDGDGSTTYMICVAAMMPLYKRIGMNPLVMTCLMLLSSGVMNLTPW 167
TV L T+T + +TT + V ++ + K +G++PL++ + + L P
Sbjct 431 APTVCLIAATITEFTSNNATTTLL--VPLLIEIAKNMGIHPLLLMVPGAIGAQFAFLLPT 488
Query 168 GGPT-ARAASALQVDASHVFIPMIPSMVIAAAWLFVLAYMYGKYERKRLG 216
G P+ + ++ + +P + +L +L G Y +G
Sbjct 489 GTPSNVVGFTTGHIEIKDMIKTGLPLKIAGTIFLSILMPTLGAYVFASMG 538
Score = 19.6 bits (39), Expect = 0.83
Identities = 21/77 (27%), Positives = 32/77 (42%), Gaps = 14/77 (18%)
Query 244 WFNGILTIVLMAALVKGILPMAILFMLGFCIALIVNYPDLQMQKKRIAMHADSVLAVVGV 303
W + + V+M + GIL + V +P A+ S V+GV
Sbjct 178 WMHNVAAAVMMMPVATGILQR----LPSSSSTTEVVHP---------AVGKFSRAVVLGV 224
Query 304 IFAAGV-FTGILTGTGM 319
I++A V LTGTG+
Sbjct 225 IYSAAVGGMSTLTGTGV 241
Score = 16.5 bits (31), Expect = 7.5
Identities = 7/12 (58%), Positives = 8/12 (67%), Gaps = 0/12 (0%)
Query 118 LTVSLDGDGSTT 129
L V L GD +TT
Sbjct 60 LCVDLGGDETTT 71
Score = 16.5 bits (31), Expect = 7.5
Identities = 17/84 (20%), Positives = 39/84 (46%), Gaps = 19/84 (23%)
Query 97 WILKKVKGDPLKITLGT---VFLTLTVSLDGDGSTTYMICVAAMM-------------PL 140
W+ + V P+ IT T +F +S D + +YM V +++ +
Sbjct 86 WLTEAV---PMPITSMTPLFLFPLFGISAADDVANSYMDDVISLVLGSFILALAVEHYNI 142
Query 141 YKRIGMNPLVMTCLMLLSSGVMNL 164
++R+ +N ++ C+ L++ ++ L
Sbjct 143 HRRLALNITLVFCVEPLNAPLLLL 166
Lambda K H a alpha
0.331 0.143 0.436 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 675945
Database: 803c12b249bcbf6f6fcd5083122f1bca.SwissProt.fasta
Posted date: May 8, 2024 5:58 PM
Number of letters in database: 1,837
Number of sequences in database: 4
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
