BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: e6fccc5ae6d73fcc2d4468d7b16c524e.SwissProt.fasta
17 sequences; 6,597 total letters
Query= ACIAD1221
Length=378
Score E
Sequences producing significant alignments: (Bits) Value
Q6FCW2 tRNA-specific 2-thiouridylase MnmA [Acinetobacter baylyi (... 778 0.0
B0VUD6 tRNA-specific 2-thiouridylase MnmA [Acinetobacter baumanni... 716 0.0
B0VBY5 tRNA-specific 2-thiouridylase MnmA [Acinetobacter baumanni... 716 0.0
A3M7G3 tRNA-specific 2-thiouridylase MnmA [Acinetobacter baumanni... 716 0.0
B7I4K8 tRNA-specific 2-thiouridylase MnmA [Acinetobacter baumanni... 716 0.0
B7GZ21 tRNA-specific 2-thiouridylase MnmA [Acinetobacter baumanni... 716 0.0
B2HVN5 tRNA-specific 2-thiouridylase MnmA [Acinetobacter baumanni... 713 0.0
A5WE20 tRNA-specific 2-thiouridylase MnmA [Psychrobacter sp. (str... 556 0.0
Q1QBM4 tRNA-specific 2-thiouridylase MnmA [Psychrobacter cryohalo... 551 0.0
Q4FSB4 tRNA-specific 2-thiouridylase MnmA [Psychrobacter arcticus... 550 0.0
Q4K9U2 tRNA-specific 2-thiouridylase MnmA [Pseudomonas fluorescen... 521 0.0
C3JY68 tRNA-specific 2-thiouridylase MnmA [Pseudomonas fluorescen... 518 0.0
A4XUY9 tRNA-specific 2-thiouridylase MnmA [Pseudomonas mendocina ... 518 0.0
P25745 tRNA-specific 2-thiouridylase MnmA [Escherichia coli (stra... 480 8e-174
O75648 Mitochondrial tRNA-specific 2-thiouridylase 1 [Homo sapiens] 246 2e-81
Q9DAT5 Mitochondrial tRNA-specific 2-thiouridylase 1 [Mus musculus] 240 5e-79
Q12093 Mitochondrial tRNA-specific 2-thiouridylase 1 [Saccharomyc... 229 1e-74
>Q6FCW2 tRNA-specific 2-thiouridylase MnmA [Acinetobacter baylyi
(strain ATCC 33305 / BD413 / ADP1)]
Length=377
Score = 778 bits (2008), Expect = 0.0
Identities = 377/377 (100%), Positives = 377/377 (100%), Gaps = 0/377 (0%)
Query 1 MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVAD 60
MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVAD
Sbjct 1 MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVAD 60
Query 61 KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMTLGADFI 120
KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMTLGADFI
Sbjct 61 KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMTLGADFI 120
Query 121 ATGHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEV 180
ATGHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEV
Sbjct 121 ATGHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEV 180
Query 181 RKIAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY 240
RKIAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY
Sbjct 181 RKIAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY 240
Query 241 TLGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDWVAGE 300
TLGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDWVAGE
Sbjct 241 TLGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDWVAGE 300
Query 301 QDIPASGFRCTAKTRYRQPDQACTIYRDEDRNNGVRVEFDEPQRAVTPGQSVVFYTNEIC 360
QDIPASGFRCTAKTRYRQPDQACTIYRDEDRNNGVRVEFDEPQRAVTPGQSVVFYTNEIC
Sbjct 301 QDIPASGFRCTAKTRYRQPDQACTIYRDEDRNNGVRVEFDEPQRAVTPGQSVVFYTNEIC 360
Query 361 LGGGVILHTDAPTPDFI 377
LGGGVILHTDAPTPDFI
Sbjct 361 LGGGVILHTDAPTPDFI 377
>B0VUD6 tRNA-specific 2-thiouridylase MnmA [Acinetobacter baumannii
(strain SDF)]
Length=377
Score = 716 bits (1849), Expect = 0.0
Identities = 339/377 (90%), Positives = 364/377 (97%), Gaps = 0/377 (0%)
Query 1 MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVAD 60
MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTA+EDLADAQAVAD
Sbjct 1 MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTAMEDLADAQAVAD 60
Query 61 KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMTLGADFI 120
KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAF+DHAMTLGADFI
Sbjct 61 KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFLDHAMTLGADFI 120
Query 121 ATGHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEV 180
ATGHY RR E+ NS+GE+YAPLLRG+DNNKDQ+YFLHAVHGREINKTLFPVGEIEKPEV
Sbjct 121 ATGHYARRAETAYNSKGEAYAPLLRGLDNNKDQTYFLHAVHGREINKTLFPVGEIEKPEV 180
Query 181 RKIAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY 240
R+IAE+LDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY
Sbjct 181 RRIAEELDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY 240
Query 241 TLGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDWVAGE 300
TLGQRGGIGLGG+KGA+EGAWFVLHKD+ NNRLV+GQGH+HPLMQST LWS+ IDWVAGE
Sbjct 241 TLGQRGGIGLGGMKGASEGAWFVLHKDVTNNRLVVGQGHDHPLMQSTQLWSESIDWVAGE 300
Query 301 QDIPASGFRCTAKTRYRQPDQACTIYRDEDRNNGVRVEFDEPQRAVTPGQSVVFYTNEIC 360
Q+IPA G RCTAKTRYRQPDQACT++ DE+ +GVRVEFDEPQRAVTPGQSVVFY++E+C
Sbjct 301 QNIPAEGLRCTAKTRYRQPDQACTVFIDENSEHGVRVEFDEPQRAVTPGQSVVFYSDEVC 360
Query 361 LGGGVILHTDAPTPDFI 377
LGGGVI HT+APTP+FI
Sbjct 361 LGGGVIHHTNAPTPNFI 377
>B0VBY5 tRNA-specific 2-thiouridylase MnmA [Acinetobacter baumannii
(strain AYE)]
Length=377
Score = 716 bits (1847), Expect = 0.0
Identities = 339/377 (90%), Positives = 364/377 (97%), Gaps = 0/377 (0%)
Query 1 MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVAD 60
MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTA+EDLADAQAVAD
Sbjct 1 MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTAMEDLADAQAVAD 60
Query 61 KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMTLGADFI 120
KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAF+DHAMTLGADFI
Sbjct 61 KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFLDHAMTLGADFI 120
Query 121 ATGHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEV 180
ATGHY RR E+ NS+GE+YAPLLRG+DNNKDQ+YFLHAVHGREINKTLFPVGEIEKPEV
Sbjct 121 ATGHYARRAETAYNSKGEAYAPLLRGLDNNKDQTYFLHAVHGREINKTLFPVGEIEKPEV 180
Query 181 RKIAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY 240
R+IAE+LDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY
Sbjct 181 RRIAEELDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY 240
Query 241 TLGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDWVAGE 300
TLGQRGGIGLGG+KGA+EGAWFVLHKD+ NNRLV+GQGH+HPLMQST LWS+ IDWVAGE
Sbjct 241 TLGQRGGIGLGGMKGASEGAWFVLHKDVANNRLVVGQGHDHPLMQSTQLWSEAIDWVAGE 300
Query 301 QDIPASGFRCTAKTRYRQPDQACTIYRDEDRNNGVRVEFDEPQRAVTPGQSVVFYTNEIC 360
Q+IPA G RCTAKTRYRQPDQACT++ DE+ +GVRVEFDEPQRAVTPGQSVVFY++E+C
Sbjct 301 QNIPAEGLRCTAKTRYRQPDQACTVFIDENSEHGVRVEFDEPQRAVTPGQSVVFYSDEVC 360
Query 361 LGGGVILHTDAPTPDFI 377
LGGGVI HT+APTP+FI
Sbjct 361 LGGGVIHHTNAPTPNFI 377
>A3M7G3 tRNA-specific 2-thiouridylase MnmA [Acinetobacter baumannii
(strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755
/ 5377)]
Length=377
Score = 716 bits (1847), Expect = 0.0
Identities = 339/377 (90%), Positives = 364/377 (97%), Gaps = 0/377 (0%)
Query 1 MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVAD 60
MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTA+EDLADAQAVAD
Sbjct 1 MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTAMEDLADAQAVAD 60
Query 61 KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMTLGADFI 120
KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAF+DHAMTLGADFI
Sbjct 61 KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFLDHAMTLGADFI 120
Query 121 ATGHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEV 180
ATGHY RR E+ NS+GE+YAPLLRG+DNNKDQ+YFLHAVHGREINKTLFPVGEIEKPEV
Sbjct 121 ATGHYARRAETAYNSKGEAYAPLLRGLDNNKDQTYFLHAVHGREINKTLFPVGEIEKPEV 180
Query 181 RKIAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY 240
R+IAE+LDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY
Sbjct 181 RRIAEELDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY 240
Query 241 TLGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDWVAGE 300
TLGQRGGIGLGG+KGA+EGAWFVLHKD+ NNRLV+GQGH+HPLMQST LWS+ IDWVAGE
Sbjct 241 TLGQRGGIGLGGMKGASEGAWFVLHKDVANNRLVVGQGHDHPLMQSTQLWSEAIDWVAGE 300
Query 301 QDIPASGFRCTAKTRYRQPDQACTIYRDEDRNNGVRVEFDEPQRAVTPGQSVVFYTNEIC 360
Q+IPA G RCTAKTRYRQPDQACT++ DE+ +GVRVEFDEPQRAVTPGQSVVFY++E+C
Sbjct 301 QNIPAEGLRCTAKTRYRQPDQACTVFIDENSEHGVRVEFDEPQRAVTPGQSVVFYSDEVC 360
Query 361 LGGGVILHTDAPTPDFI 377
LGGGVI HT+APTP+FI
Sbjct 361 LGGGVIHHTNAPTPNFI 377
>B7I4K8 tRNA-specific 2-thiouridylase MnmA [Acinetobacter baumannii
(strain AB0057)]
Length=377
Score = 716 bits (1847), Expect = 0.0
Identities = 339/377 (90%), Positives = 364/377 (97%), Gaps = 0/377 (0%)
Query 1 MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVAD 60
MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTA+EDLADAQAVAD
Sbjct 1 MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTAMEDLADAQAVAD 60
Query 61 KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMTLGADFI 120
KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAF+DHAMTLGADFI
Sbjct 61 KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFLDHAMTLGADFI 120
Query 121 ATGHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEV 180
ATGHY RR E+ NS+GE+YAPLLRG+DNNKDQ+YFLHAVHGREINKTLFPVGEIEKPEV
Sbjct 121 ATGHYARRAETAYNSKGEAYAPLLRGLDNNKDQTYFLHAVHGREINKTLFPVGEIEKPEV 180
Query 181 RKIAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY 240
R+IAE+LDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY
Sbjct 181 RRIAEELDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY 240
Query 241 TLGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDWVAGE 300
TLGQRGGIGLGG+KGA+EGAWFVLHKD+ NNRLV+GQGH+HPLMQST LWS+ IDWVAGE
Sbjct 241 TLGQRGGIGLGGMKGASEGAWFVLHKDVANNRLVVGQGHDHPLMQSTQLWSEAIDWVAGE 300
Query 301 QDIPASGFRCTAKTRYRQPDQACTIYRDEDRNNGVRVEFDEPQRAVTPGQSVVFYTNEIC 360
Q+IPA G RCTAKTRYRQPDQACT++ DE+ +GVRVEFDEPQRAVTPGQSVVFY++E+C
Sbjct 301 QNIPAEGLRCTAKTRYRQPDQACTVFIDENSEHGVRVEFDEPQRAVTPGQSVVFYSDEVC 360
Query 361 LGGGVILHTDAPTPDFI 377
LGGGVI HT+APTP+FI
Sbjct 361 LGGGVIHHTNAPTPNFI 377
>B7GZ21 tRNA-specific 2-thiouridylase MnmA [Acinetobacter baumannii
(strain AB307-0294)]
Length=377
Score = 716 bits (1847), Expect = 0.0
Identities = 339/377 (90%), Positives = 364/377 (97%), Gaps = 0/377 (0%)
Query 1 MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVAD 60
MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTA+EDLADAQAVAD
Sbjct 1 MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTAMEDLADAQAVAD 60
Query 61 KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMTLGADFI 120
KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAF+DHAMTLGADFI
Sbjct 61 KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFLDHAMTLGADFI 120
Query 121 ATGHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEV 180
ATGHY RR E+ NS+GE+YAPLLRG+DNNKDQ+YFLHAVHGREINKTLFPVGEIEKPEV
Sbjct 121 ATGHYARRAETAYNSKGEAYAPLLRGLDNNKDQTYFLHAVHGREINKTLFPVGEIEKPEV 180
Query 181 RKIAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY 240
R+IAE+LDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY
Sbjct 181 RRIAEELDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY 240
Query 241 TLGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDWVAGE 300
TLGQRGGIGLGG+KGA+EGAWFVLHKD+ NNRLV+GQGH+HPLMQST LWS+ IDWVAGE
Sbjct 241 TLGQRGGIGLGGMKGASEGAWFVLHKDVANNRLVVGQGHDHPLMQSTQLWSEAIDWVAGE 300
Query 301 QDIPASGFRCTAKTRYRQPDQACTIYRDEDRNNGVRVEFDEPQRAVTPGQSVVFYTNEIC 360
Q+IPA G RCTAKTRYRQPDQACT++ DE+ +GVRVEFDEPQRAVTPGQSVVFY++E+C
Sbjct 301 QNIPAEGLRCTAKTRYRQPDQACTVFIDENSEHGVRVEFDEPQRAVTPGQSVVFYSDEVC 360
Query 361 LGGGVILHTDAPTPDFI 377
LGGGVI HT+APTP+FI
Sbjct 361 LGGGVIHHTNAPTPNFI 377
>B2HVN5 tRNA-specific 2-thiouridylase MnmA [Acinetobacter baumannii
(strain ACICU)]
Length=377
Score = 713 bits (1841), Expect = 0.0
Identities = 338/377 (90%), Positives = 363/377 (96%), Gaps = 0/377 (0%)
Query 1 MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVAD 60
MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTA+EDLADAQAVAD
Sbjct 1 MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTAMEDLADAQAVAD 60
Query 61 KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMTLGADFI 120
KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAF+DHAMTLGADFI
Sbjct 61 KIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFLDHAMTLGADFI 120
Query 121 ATGHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEV 180
ATGHY RR E+ NS+GE+YAPLLRG+D NKDQ+YFLHAVHGREINKTLFPVGEIEKPEV
Sbjct 121 ATGHYARRAETAYNSKGEAYAPLLRGLDKNKDQTYFLHAVHGREINKTLFPVGEIEKPEV 180
Query 181 RKIAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY 240
R+IAE+LDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY
Sbjct 181 RRIAEELDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYY 240
Query 241 TLGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDWVAGE 300
TLGQRGGIGLGG+KGA+EGAWFVLHKD+ NNRLV+GQGH+HPLMQST LWS+ IDWVAGE
Sbjct 241 TLGQRGGIGLGGMKGASEGAWFVLHKDVANNRLVVGQGHDHPLMQSTQLWSEAIDWVAGE 300
Query 301 QDIPASGFRCTAKTRYRQPDQACTIYRDEDRNNGVRVEFDEPQRAVTPGQSVVFYTNEIC 360
Q+IPA G RCTAKTRYRQPDQACT++ DE+ +GVRVEFDEPQRAVTPGQSVVFY++E+C
Sbjct 301 QNIPAEGLRCTAKTRYRQPDQACTVFIDENSEHGVRVEFDEPQRAVTPGQSVVFYSDEVC 360
Query 361 LGGGVILHTDAPTPDFI 377
LGGGVI HT+APTP+FI
Sbjct 361 LGGGVIHHTNAPTPNFI 377
>A5WE20 tRNA-specific 2-thiouridylase MnmA [Psychrobacter sp.
(strain PRwf-1)]
Length=398
Score = 556 bits (1433), Expect = 0.0
Identities = 269/370 (73%), Positives = 307/370 (83%), Gaps = 7/370 (2%)
Query 2 QQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVADK 61
Q RV+VGMSGGVDSSVSA LL Q G+QVEGLFMKNWEEDDGTEYCTA+EDLADAQAV DK
Sbjct 27 QTRVVVGMSGGVDSSVSAVLLQQAGFQVEGLFMKNWEEDDGTEYCTAMEDLADAQAVCDK 86
Query 62 IGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMTLGADFIA 121
IGI LHTANFAMEYWDRVFEHFLAEY AGRTPNPDILCNKEIKF+AF+D+A+TLGAD+IA
Sbjct 87 IGINLHTANFAMEYWDRVFEHFLAEYQAGRTPNPDILCNKEIKFKAFLDYAVTLGADYIA 146
Query 122 TGHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEVR 181
TGHYTRR + G A L+RG+DNNKDQSYFLHAV G +I KTLFPVGE+EKPEVR
Sbjct 147 TGHYTRRSVNYTREDGLEVAHLMRGLDNNKDQSYFLHAVGGDKIAKTLFPVGELEKPEVR 206
Query 182 KIAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYYT 241
+IAE+ DL TAKKKDSTGICFIGERRF DFL+QYLPA+PG IV D+G +G+H GLMYYT
Sbjct 207 RIAEEHDLITAKKKDSTGICFIGERRFKDFLQQYLPAKPGNIVTDDGITIGKHDGLMYYT 266
Query 242 LGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDWVAGEQ 301
LGQRGGIG+GG+K E WFVL KDL NN L++GQGH+HP++ S L + +DWV E+
Sbjct 267 LGQRGGIGIGGVKDRPEEPWFVLQKDLSNNELIVGQGHDHPMLLSQRLTAYKLDWV--ER 324
Query 302 DIPAS-----GFRCTAKTRYRQPDQACTIYRDEDRNNGVRVEFDEPQRAVTPGQSVVFYT 356
PA+ G +C AKTRYRQPDQ+CT++ D D + V+V FDEPQRAVTPGQS VFY
Sbjct 325 IAPAAIFSEQGLKCMAKTRYRQPDQSCTVFADSDDGSRVKVIFDEPQRAVTPGQSAVFYI 384
Query 357 NEICLGGGVI 366
E+CLGGGVI
Sbjct 385 GEVCLGGGVI 394
>Q1QBM4 tRNA-specific 2-thiouridylase MnmA [Psychrobacter cryohalolentis
(strain ATCC BAA-1226 / DSM 17306 / VKM B-2378 /
K5)]
Length=408
Score = 551 bits (1421), Expect = 0.0
Identities = 269/373 (72%), Positives = 307/373 (82%), Gaps = 3/373 (1%)
Query 3 QRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVADKI 62
+ VIVGMSGGVDSSVSA LL Q G+ VEGLFMKNWEEDDGTEYCTA++DLADAQAV DKI
Sbjct 33 KHVIVGMSGGVDSSVSAVLLQQAGFMVEGLFMKNWEEDDGTEYCTAMDDLADAQAVCDKI 92
Query 63 GIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMTLGADFIAT 122
GIKLHTANFAMEYWDRVFEHFLAEY AGRTPNPDILCNKEIKF+AF+D+A+TLGAD+IAT
Sbjct 93 GIKLHTANFAMEYWDRVFEHFLAEYKAGRTPNPDILCNKEIKFKAFLDYALTLGADYIAT 152
Query 123 GHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEVRK 182
GHYTRR + +N+ G A LLRG+DNNKDQSYFLHAV G ++ KTLFPVGE++KP VR+
Sbjct 153 GHYTRRSVNYKNNDGIEVAQLLRGLDNNKDQSYFLHAVGGDKLAKTLFPVGELKKPVVRQ 212
Query 183 IAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYYTL 242
IAE+ DL TA KKDSTGICFIGERRF DFL+QYLPAQ G IV D+G +G H GLMYYTL
Sbjct 213 IAEEHDLITANKKDSTGICFIGERRFKDFLQQYLPAQKGDIVTDDGITIGTHDGLMYYTL 272
Query 243 GQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDW--VAGE 300
GQRGGIG+GG+K E WFVL KDL+NNRL++GQGHEH +M S L + +DW V
Sbjct 273 GQRGGIGIGGVKDRPEEPWFVLAKDLDNNRLIVGQGHEHAMMLSNELQAYKLDWVDVLPP 332
Query 301 QDI-PASGFRCTAKTRYRQPDQACTIYRDEDRNNGVRVEFDEPQRAVTPGQSVVFYTNEI 359
DI G RC AK+RYRQPDQACT++ + + + VRV FDEPQRAVTPGQS VFY +E+
Sbjct 333 ADIFSEDGLRCMAKSRYRQPDQACTVFANNENGSEVRVVFDEPQRAVTPGQSAVFYIDEV 392
Query 360 CLGGGVILHTDAP 372
CLGGGVI DAP
Sbjct 393 CLGGGVIASIDAP 405
>Q4FSB4 tRNA-specific 2-thiouridylase MnmA [Psychrobacter arcticus
(strain DSM 17307 / VKM B-2377 / 273-4)]
Length=408
Score = 550 bits (1417), Expect = 0.0
Identities = 270/373 (72%), Positives = 304/373 (82%), Gaps = 3/373 (1%)
Query 3 QRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVADKI 62
+ VIVGMSGGVDSSVSA LL Q G+ VEGLFMKNWEEDDGTEYCTA++DLADAQAV DKI
Sbjct 33 KHVIVGMSGGVDSSVSAVLLQQAGFVVEGLFMKNWEEDDGTEYCTAMDDLADAQAVCDKI 92
Query 63 GIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMTLGADFIAT 122
GIKLHTANFAMEYWDRVFEHFLAEY AGRTPNPDILCNKEIKF+AF+D+A+TLGAD+IAT
Sbjct 93 GIKLHTANFAMEYWDRVFEHFLAEYKAGRTPNPDILCNKEIKFKAFLDYALTLGADYIAT 152
Query 123 GHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEVRK 182
GHYTRR + +N+ G A LLRG+DNNKDQSYFLHAV G ++ KTLFPVGE+EKP VR+
Sbjct 153 GHYTRRSVNYKNNDGIEVAQLLRGLDNNKDQSYFLHAVGGDKLAKTLFPVGELEKPVVRQ 212
Query 183 IAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYYTL 242
IAE+ DL TA KKDSTGICFIGERRF DFL+QYLPAQ G IV D+G +G H GLMYYTL
Sbjct 213 IAEEHDLITANKKDSTGICFIGERRFKDFLQQYLPAQKGDIVTDDGINIGTHDGLMYYTL 272
Query 243 GQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDWVAG--E 300
GQRGGIG+GG+K E WFVL KDL+NNRL++GQGHEHP+M S L + +DW G
Sbjct 273 GQRGGIGIGGVKDRPEEPWFVLAKDLDNNRLIVGQGHEHPMMLSNELHAYKLDWTDGLPP 332
Query 301 QDI-PASGFRCTAKTRYRQPDQACTIYRDEDRNNGVRVEFDEPQRAVTPGQSVVFYTNEI 359
DI G RC AK+RYRQPDQAC ++ + VRV FDEPQRAVTPGQS VFY E+
Sbjct 333 TDIFSLEGLRCMAKSRYRQPDQACRVFAVNADGSEVRVIFDEPQRAVTPGQSAVFYIEEV 392
Query 360 CLGGGVILHTDAP 372
CLGGGVI DAP
Sbjct 393 CLGGGVIASIDAP 405
>Q4K9U2 tRNA-specific 2-thiouridylase MnmA [Pseudomonas fluorescens
(strain ATCC BAA-477 / NRRL B-23932 / Pf-5)]
Length=377
Score = 521 bits (1342), Expect = 0.0
Identities = 260/366 (71%), Positives = 296/366 (81%), Gaps = 13/366 (4%)
Query 2 QQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVADK 61
++RVIVGMSGGVDSSVSA LL++QGYQVEGLFMKNWEEDDGTEYCTA++DLADAQAV DK
Sbjct 11 KKRVIVGMSGGVDSSVSALLLMEQGYQVEGLFMKNWEEDDGTEYCTAMDDLADAQAVCDK 70
Query 62 IGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMTLGADFIA 121
IGIKLHTANFA EYWD VFEHFLAEY AGRTPNPDILCN+EIKF+AF+D+AM LGAD IA
Sbjct 71 IGIKLHTANFAAEYWDNVFEHFLAEYKAGRTPNPDILCNREIKFKAFLDYAMMLGADLIA 130
Query 122 TGHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEVR 181
TGHY RR + + LL+G+D NKDQSYFLHAV G +I KTLFPVGE+EKPEVR
Sbjct 131 TGHYVRRRDI------DGRTELLKGLDPNKDQSYFLHAVGGEQIAKTLFPVGELEKPEVR 184
Query 182 KIAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYYT 241
IAEK DLATAKKKDSTGICFIGERRF+DFLKQYLPAQPG+I G+ +G HHGLMY+T
Sbjct 185 AIAEKHDLATAKKKDSTGICFIGERRFSDFLKQYLPAQPGEIKTTEGEVIGRHHGLMYHT 244
Query 242 LGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDWVAG-E 300
+GQR G+G+GGLK A E W+VL KDLE+N L++GQG++HP + S L + DI WV +
Sbjct 245 IGQRQGLGIGGLKDAGEEPWYVLIKDLEHNELIVGQGNDHPWLFSRALLASDIYWVNPID 304
Query 301 QDIPASGFRCTAKTRYRQPDQACTIYRDEDRNNGVRVEFDEPQRAVTPGQSVVFYTNEIC 360
+P R TAK RYRQ DQ CT+ E NG R FD+PQRAVTPGQSVVFY EIC
Sbjct 305 LSLPR---RLTAKVRYRQSDQPCTL---EKTTNGYRATFDDPQRAVTPGQSVVFYDGEIC 358
Query 361 LGGGVI 366
LGGGVI
Sbjct 359 LGGGVI 364
>C3JY68 tRNA-specific 2-thiouridylase MnmA [Pseudomonas fluorescens
(strain SBW25)]
Length=374
Score = 518 bits (1333), Expect = 0.0
Identities = 259/365 (71%), Positives = 298/365 (82%), Gaps = 11/365 (3%)
Query 2 QQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVADK 61
++RVIVGMSGGVDSSVSA LL++QGY+VEGLFMKNWEEDDGTEYCTA++DLADAQAV DK
Sbjct 11 KKRVIVGMSGGVDSSVSAVLLMEQGYEVEGLFMKNWEEDDGTEYCTAMDDLADAQAVCDK 70
Query 62 IGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMTLGADFIA 121
IGIKLHTANFA EYWD VFEHFLAEY AGRTPNPDILCN+EIKF+AF+D+AM LGAD IA
Sbjct 71 IGIKLHTANFAAEYWDNVFEHFLAEYKAGRTPNPDILCNREIKFKAFLDYAMMLGADLIA 130
Query 122 TGHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEVR 181
TGHY RR + + LL+G+D NKDQSYFLHAV G +I KTLFPVGE+EKPEVR
Sbjct 131 TGHYVRRRDI------DGRTELLKGLDPNKDQSYFLHAVGGEQIAKTLFPVGELEKPEVR 184
Query 182 KIAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYYT 241
KIAEK LATAKKKDSTGICFIGERRF+DFLKQYLPAQPG+I +G+ +G HHGLMY+T
Sbjct 185 KIAEKHGLATAKKKDSTGICFIGERRFSDFLKQYLPAQPGEIKTTDGEVIGRHHGLMYHT 244
Query 242 LGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDWVAGEQ 301
+GQR G+G+GGLK A E W+VL KDL+NN L++GQG+EHPL+ S L + +I WV
Sbjct 245 IGQRQGLGIGGLKDAGEEPWYVLVKDLDNNVLIVGQGNEHPLLFSGALLASEIYWV-NPI 303
Query 302 DIPASGFRCTAKTRYRQPDQACTIYRDEDRNNGVRVEFDEPQRAVTPGQSVVFYTNEICL 361
D+ ++ R TAK RYRQ DQ CT+ E G R FD+PQRAVTPGQSVVFY EICL
Sbjct 304 DL-STPRRLTAKVRYRQGDQPCTL---EKTATGYRATFDDPQRAVTPGQSVVFYDGEICL 359
Query 362 GGGVI 366
GGGVI
Sbjct 360 GGGVI 364
>A4XUY9 tRNA-specific 2-thiouridylase MnmA [Pseudomonas mendocina
(strain ymp)]
Length=370
Score = 518 bits (1333), Expect = 0.0
Identities = 256/364 (70%), Positives = 297/364 (82%), Gaps = 11/364 (3%)
Query 3 QRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVADKI 62
QRVIVGMSGGVDSSVSA LL++QGYQVEGLFMKNW+EDDGTEYCTA++DLADAQAV D+I
Sbjct 7 QRVIVGMSGGVDSSVSALLLMEQGYQVEGLFMKNWDEDDGTEYCTAMDDLADAQAVCDRI 66
Query 63 GIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMTLGADFIAT 122
GIKLHTANFA EYWD VFEHFLAEY AGRTPNPDILCN+EIKF+AF+D+A++LGAD IAT
Sbjct 67 GIKLHTANFAAEYWDNVFEHFLAEYKAGRTPNPDILCNREIKFKAFLDYALSLGADLIAT 126
Query 123 GHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEVRK 182
GHY RR + + LL+G+D NKDQSYFLHAV G +I KTLFPVGE+EKP+VR
Sbjct 127 GHYVRRRDI------DGRTELLKGLDPNKDQSYFLHAVGGEQIAKTLFPVGELEKPQVRA 180
Query 183 IAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYYTL 242
IAEK +LATAKKKDSTGICFIGERRF+DFLKQYLPAQPG I G+ +G HHGLMY+T+
Sbjct 181 IAEKYELATAKKKDSTGICFIGERRFSDFLKQYLPAQPGDIETTEGQVIGRHHGLMYHTI 240
Query 243 GQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDWVAGEQD 302
GQR G+G+GGLK A++ W+VL KDL N L++GQG+EHP + S L + DI WV D
Sbjct 241 GQRQGLGIGGLKDASDDPWYVLRKDLTRNVLIVGQGNEHPWLFSRALLASDIYWV-NPVD 299
Query 303 IPASGFRCTAKTRYRQPDQACTIYRDEDRNNGVRVEFDEPQRAVTPGQSVVFYTNEICLG 362
+ ++ R TAK RYRQ DQACT+ + E NG R FDEPQRAVTPGQSVVFY E+CLG
Sbjct 300 L-STPRRLTAKVRYRQSDQACTLEKTE---NGYRAVFDEPQRAVTPGQSVVFYDGEVCLG 355
Query 363 GGVI 366
GGVI
Sbjct 356 GGVI 359
>P25745 tRNA-specific 2-thiouridylase MnmA [Escherichia coli (strain
K12)]
Length=368
Score = 480 bits (1235), Expect = 8e-174
Identities = 244/367 (66%), Positives = 284/367 (77%), Gaps = 15/367 (4%)
Query 3 QRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVADKI 62
++VIVGMSGGVDSSVSA LL QQGYQVEGLFMKNWEEDDG EYCTA DLADAQAV DK+
Sbjct 6 KKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDDGEEYCTAAADLADAQAVCDKL 65
Query 63 GIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAMT-LGADFIA 121
GI+LHT NFA EYWD VFE FLAEY AGRTPNPDILCNKEIKF+AF++ A LGAD+IA
Sbjct 66 GIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILCNKEIKFKAFLEFAAEDLGADYIA 125
Query 122 TGHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIEKPEVR 181
TGHY RR + SR LLRG+D+NKDQSYFL+ + +I ++LFPVGE+EKP+VR
Sbjct 126 TGHYVRRADVDGKSR------LLRGLDSNKDQSYFLYTLSHEQIAQSLFPVGELEKPQVR 179
Query 182 KIAEKLDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYYT 241
KIAE L L TAKKKDSTGICFIGER+F +FL +YLPAQPGKI+ +G E+GEH GLMY+T
Sbjct 180 KIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQPGKIITVDGDEIGEHQGLMYHT 239
Query 242 LGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTTLWSQDIDWVAGEQ 301
LGQR G+G+GG K E W+V+ KD+ENN LV+ QGHEHP + S L +Q + WV E
Sbjct 240 LGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQGHEHPRLMSVGLIAQQLHWVDRE- 298
Query 302 DIPASG-FRCTAKTRYRQPDQACTIYR-DEDRNNGVRVEFDEPQRAVTPGQSVVFYTNEI 359
P +G RCT KTRYRQ D CT+ D+DR + V FDEP AVTPGQS VFY E+
Sbjct 299 --PFTGTMRCTVKTRYRQTDIPCTVKALDDDR---IEVIFDEPVAAVTPGQSAVFYNGEV 353
Query 360 CLGGGVI 366
CLGGG+I
Sbjct 354 CLGGGII 360
>O75648 Mitochondrial tRNA-specific 2-thiouridylase 1 [Homo sapiens]
Length=421
Score = 246 bits (628), Expect = 2e-81
Identities = 152/390 (39%), Positives = 214/390 (55%), Gaps = 35/390 (9%)
Query 3 QRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVADKI 62
+ V+ +SGGVDS+V+A LL ++GYQV G+FMKNW+ D CTA +D DA V +
Sbjct 5 RHVVCALSGGVDSAVAALLLRRRGYQVTGVFMKNWDSLDEHGVCTADKDCEDAYRVCQIL 64
Query 63 GIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAM-TLGADFIA 121
I H ++ EYW+ VF FL EY GRTPNPDI+CNK IKF F +A+ LGAD IA
Sbjct 65 DIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDIVCNKHIKFSCFFHYAVDNLGADAIA 124
Query 122 TGHYTR---------------RGESMQNSRGE--SYAPLLRGVDNNKDQSYFLHAVHGRE 164
TGHY R + E + +R E + LL+ D+ KDQ++FL V
Sbjct 125 TGHYARTSLEDEEVFEQKHVKKPEGLFRNRFEVRNAVKLLQAADSFKDQTFFLSQVSQDA 184
Query 165 INKTLFPVGEIEKPEVRKIAEKLDL-ATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKI 223
+ +T+FP+G + K V+KIA + L +KK+S G+CFIG+R F FL QYL +PG
Sbjct 185 LRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHF 244
Query 224 V-LDNGKEVGEHHGLMYYTLGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHP 282
+ +++ K +G H G YTLGQR I GGL+ W+V+ KD + + +HP
Sbjct 245 ISIEDNKVLGTHKGWFLYTLGQRANI--GGLR----EPWYVVEKDSVKGDVFVAPRTDHP 298
Query 283 LMQSTTLWSQDIDWVAGEQDIPASGFR-----CTAKTRYRQPDQACTIYRDEDRNNGVRV 337
+ L + + W+A E PA+ R C + R++ C + ++D V V
Sbjct 299 ALYRDLLRTSRVHWIAEEP--PAALVRDKMMECHFRFRHQMALVPCVLTLNQD--GTVWV 354
Query 338 EFDEPQRAVTPGQSVVFYTNEICLGGGVIL 367
+ RA+ GQ VFY + CLG G IL
Sbjct 355 TAVQAVRALATGQFAVFYKGDECLGSGKIL 384
>Q9DAT5 Mitochondrial tRNA-specific 2-thiouridylase 1 [Mus musculus]
Length=417
Score = 240 bits (612), Expect = 5e-79
Identities = 150/390 (38%), Positives = 212/390 (54%), Gaps = 35/390 (9%)
Query 3 QRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTALEDLADAQAVADKI 62
+ V+ +SGGVDS+V+A LL ++GYQV G+FMKNW+ D C A +D DA V +
Sbjct 5 RHVVCALSGGVDSAVAALLLRRRGYQVTGVFMKNWDSLDEQGVCAADKDCEDAYKVCQIL 64
Query 63 GIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFIDHAM-TLGADFIA 121
I H ++ EYW+ VF FL EY GRTPNPDI CNK IKF F +A+ LGAD +A
Sbjct 65 DIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDINCNKHIKFSCFYHYAVDNLGADAVA 124
Query 122 TGHY---------------TRRGESMQNSRGESYAP--LLRGVDNNKDQSYFLHAVHGRE 164
TGHY T++ + + +R E P LL+ D+ KDQ++FL V
Sbjct 125 TGHYARTSLEDEEVFEQKHTKKPDGLFRNRFEVRNPVKLLQAADSFKDQTFFLSQVSQDA 184
Query 165 INKTLFPVGEIEKPEVRKIAEKLDL-ATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKI 223
+ +T+FP+GE+ K V+KIA + L +K++S GICFIG+R FL QYL +PGK
Sbjct 185 LRRTIFPLGELTKDFVKKIAAENSLHHVLQKRESMGICFIGKRNLEHFLLQYLQPRPGKF 244
Query 224 V-LDNGKEVGEHHGLMYYTLGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHP 282
V +++ +G H G YTLGQR I GL+ W+V+ KD +++ +HP
Sbjct 245 VSIEDNTVLGTHKGWFLYTLGQRAKI--SGLR----EPWYVVEKDGTKGDVLVAPRVDHP 298
Query 283 LMQSTTLWSQDIDWVAGEQDIPASGFR-----CTAKTRYRQPDQACTIYRDEDRNNGVRV 337
+ L + + W+A E PA+ R C + R++ C + ++D V V
Sbjct 299 ALYRDLLRTNRVHWIAEEP--PAALVRDKMMECHFRFRHQMALVPCVLTLNQD--GTVWV 354
Query 338 EFDEPQRAVTPGQSVVFYTNEICLGGGVIL 367
+ R + GQ VFY E CLG G IL
Sbjct 355 TAVKAVRGLALGQFAVFYKGEECLGSGKIL 384
>Q12093 Mitochondrial tRNA-specific 2-thiouridylase 1 [Saccharomyces
cerevisiae (strain ATCC 204508 / S288c)]
Length=417
Score = 229 bits (583), Expect = 1e-74
Identities = 142/389 (37%), Positives = 205/389 (53%), Gaps = 29/389 (7%)
Query 5 VIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEE-----DDGTEYCTALEDLADAQAVA 59
VIV MS GVDSSV+AAL + G++M+NW E D G E C D D VA
Sbjct 29 VIVAMSSGVDSSVAAALFAGEFPNTRGVYMQNWSESQSLDDPGKEPCYE-RDWRDVNRVA 87
Query 60 DKIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKF---RAFIDHAMTLG 116
+ I++ NF +YW VFE L Y+ G TPNPDI CNK +KF R ++D G
Sbjct 88 KHLNIRVDKVNFEQDYWIDVFEPMLRGYSEGSTPNPDIGCNKFVKFGKLREWLDEKYGTG 147
Query 117 ADFIATGHYTRRGESMQNSRGESYAPLLRGVDNNKDQSYFLHAVHGREINKTLFPVGEIE 176
++ TGHY R MQ G+ LLR + KDQSY+L ++ ++ L P+G +
Sbjct 148 NYWLVTGHYAR---VMQEMNGKGLFHLLRSIYRPKDQSYYLSQINSTVLSSLLLPIGHLT 204
Query 177 KPEVRKIAEKLDLATAKKKDSTGICFIGER---RFNDFLKQYLPAQPGKIVL---DNGKE 230
KPEVR +A+ L TA+K DS GICF+ +F +FLK YLP+ PG I+ +G +
Sbjct 205 KPEVRDLAKYAGLPTAEKPDSQGICFVNNSQHGKFKNFLKHYLPSSPGDIITVDPQSGAK 264
Query 231 V--GEHHGLMYYTLGQRGGIGLGGLKGAAEGAWFVLHKDLENNRLVIGQGHEHPLMQSTT 288
G H GL YT+GQ+ GI + +G WFV K + N ++I +G ++P + S T
Sbjct 265 TTWGRHDGLWSYTIGQKVGISMPQADPNYQGTWFVSEKLRDTNEILIVRGRDNPALYSDT 324
Query 289 LWSQDIDWVAGEQDIPASGFRCTA--KTRYRQPD-----QACTIYRDEDRNNGVRVEFDE 341
+ ++ + +D + F+ T ++R ++C + R D N + +
Sbjct 325 MRIENFSSLGPRED-TINAFQNTGALTLQFRSLQVPVQIKSCKLNRSAD-NLDITIHLAS 382
Query 342 PQRAVTPGQSVVFYTNEICLGGGVILHTD 370
QRA+TPGQS Y ++ LG G I H +
Sbjct 383 KQRAITPGQSCCLYIDDRVLGSGPISHVN 411
Lambda K H a alpha
0.321 0.139 0.425 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 1885016
Database: e6fccc5ae6d73fcc2d4468d7b16c524e.SwissProt.fasta
Posted date: May 20, 2024 1:00 PM
Number of letters in database: 6,597
Number of sequences in database: 17
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
