BLASTP 2.12.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.


Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.



Database: c57356ae72a528b92fe2901d729048f9.SwissProt.fasta
           2 sequences; 1,735 total letters



Query= ACIAD1326

Length=205
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

Q3MHT4 Speckle targeted PIP5K1A-regulated poly(A) polymerase [Rat...  37.7    5e-07
Q8R3F9 Speckle targeted PIP5K1A-regulated poly(A) polymerase [Mus...  35.8    2e-06


>Q3MHT4 Speckle targeted PIP5K1A-regulated poly(A) polymerase 
[Rattus norvegicus]
Length=866

 Score = 37.7 bits (86),  Expect = 5e-07
 Identities = 35/97 (36%), Positives = 50/97 (52%), Gaps = 7/97 (7%)

Query  37   ISAKESVEHTPKDS-SSEPILVQPDVIEIVQQPIQNPVTKQHKTIDLEISITLSQFRLEA  95
            ISA+E+V   PK S     + V+P      Q+  Q+P +K  K +D   S  L+Q   EA
Sbjct  102  ISAREAVLSQPKHSLGGHTLRVRPRE----QKEFQSPASKSPKGVDSN-SHQLAQALAEA  156

Query  96   F-VLRQSVLLVETTTLSDAERKLWTNIQSALSGTYAE  131
              V  Q V LVE   LS+AER+L T + + +   + E
Sbjct  157  ADVGAQMVKLVELRELSEAERQLRTLVVALMQEVFTE  193


 Score = 15.0 bits (27),  Expect = 9.2
 Identities = 4/20 (20%), Positives = 11/20 (55%), Gaps = 0/20 (0%)

Query  35   IPISAKESVEHTPKDSSSEP  54
            +P+   + ++ T + +  EP
Sbjct  715  LPLMTAKCLDKTAEQNPMEP  734


>Q8R3F9 Speckle targeted PIP5K1A-regulated poly(A) polymerase 
[Mus musculus]
Length=869

 Score = 35.8 bits (81),  Expect = 2e-06
 Identities = 30/96 (31%), Positives = 45/96 (47%), Gaps = 5/96 (5%)

Query  37   ISAKESVEHTPKDS-SSEPILVQPDVIEIVQQPIQNPVTKQHKTIDLEISITLSQFRLEA  95
            ISA+E+V   PK S     + V+P      Q+  Q+P +K  K +D      +      A
Sbjct  102  ISAREAVLSQPKHSLGGHGLRVRPRE----QKEFQSPASKSPKGVDSSSHQLVQALAEAA  157

Query  96   FVLRQSVLLVETTTLSDAERKLWTNIQSALSGTYAE  131
             V  Q V LVE   LS+AER+L   + + +   + E
Sbjct  158  DVGAQMVKLVELRELSEAERQLRNLVVALMQEVFTE  193



Lambda      K        H        a         alpha
   0.321    0.134    0.397    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 281047


  Database: c57356ae72a528b92fe2901d729048f9.SwissProt.fasta
    Posted date:  May 19, 2024  10:31 PM
  Number of letters in database: 1,735
  Number of sequences in database:  2



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40