BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 785afe96a10ada4ed89566892c84291c.SwissProt.fasta
12 sequences; 5,456 total letters
Query= ACIAD1436
Length=462
Score E
Sequences producing significant alignments: (Bits) Value
P07769 Benzoate 1,2-dioxygenase subunit alpha [Acinetobacter bayl... 967 0.0
P23099 Toluate 1,2-dioxygenase subunit alpha [Pseudomonas putida] 631 0.0
Q51601 2-halobenzoate 1,2-dioxygenase large subunit [Burkholderia... 545 0.0
O85673 Anthranilate 1,2-dioxygenase large subunit [Acinetobacter ... 399 4e-139
Q51974 p-cumate 2,3-dioxygenase system, large oxygenase component... 200 1e-62
Q53122 Biphenyl 2,3-dioxygenase subunit alpha [Rhodococcus jostii... 165 4e-49
P0C618 Benzene 1,2-dioxygenase subunit alpha [Pseudomonas putida] 164 4e-49
A5W4F2 Benzene 1,2-dioxygenase subunit alpha [Pseudomonas putida ... 164 4e-49
Q7N4W0 3-phenylpropionate/cinnamic acid dioxygenase subunit alpha... 164 5e-49
O24676 Chlorobenzene dioxygenase subunit alpha [Cupriavidus sp. (... 161 6e-48
Q07944 Benzene 1,2-dioxygenase subunit alpha [Pseudomonas putida] 159 6e-47
P37333 Biphenyl dioxygenase subunit alpha [Paraburkholderia xenov... 157 3e-46
>P07769 Benzoate 1,2-dioxygenase subunit alpha [Acinetobacter
baylyi (strain ATCC 33305 / BD413 / ADP1)]
Length=461
Score = 967 bits (2501), Expect = 0.0
Identities = 461/461 (100%), Positives = 461/461 (100%), Gaps = 0/461 (0%)
Query 1 MPRIPVINTSHLDRIDELLVDNTETGEFKLHRSVFTDQALFDLEMKYIFEGNWVYLAHES 60
MPRIPVINTSHLDRIDELLVDNTETGEFKLHRSVFTDQALFDLEMKYIFEGNWVYLAHES
Sbjct 1 MPRIPVINTSHLDRIDELLVDNTETGEFKLHRSVFTDQALFDLEMKYIFEGNWVYLAHES 60
Query 61 QIPNNNDYYTTYIGRQPILIARNRNGELNAMINACSHRGAQLCRHKRGNKTTYTCPFHGW 120
QIPNNNDYYTTYIGRQPILIARNRNGELNAMINACSHRGAQLCRHKRGNKTTYTCPFHGW
Sbjct 61 QIPNNNDYYTTYIGRQPILIARNRNGELNAMINACSHRGAQLCRHKRGNKTTYTCPFHGW 120
Query 121 TFNNSGKLLKVKDPSDAGYSDCFNQDGSHDLKKVARFESYKGFLFGSLNPDVPSLQEFLG 180
TFNNSGKLLKVKDPSDAGYSDCFNQDGSHDLKKVARFESYKGFLFGSLNPDVPSLQEFLG
Sbjct 121 TFNNSGKLLKVKDPSDAGYSDCFNQDGSHDLKKVARFESYKGFLFGSLNPDVPSLQEFLG 180
Query 181 ETTKIIDMIVGQSDQGLEVLRGVSTYTYEGNWKLTAENGADGYHVSAVHWNYAATTQHRK 240
ETTKIIDMIVGQSDQGLEVLRGVSTYTYEGNWKLTAENGADGYHVSAVHWNYAATTQHRK
Sbjct 181 ETTKIIDMIVGQSDQGLEVLRGVSTYTYEGNWKLTAENGADGYHVSAVHWNYAATTQHRK 240
Query 241 EKQAGDTIRAMSAGSWGKHGGGSYGFEHGHMLLWTQWGNPEDRPNFPKAAEYTEKFGAAM 300
EKQAGDTIRAMSAGSWGKHGGGSYGFEHGHMLLWTQWGNPEDRPNFPKAAEYTEKFGAAM
Sbjct 241 EKQAGDTIRAMSAGSWGKHGGGSYGFEHGHMLLWTQWGNPEDRPNFPKAAEYTEKFGAAM 300
Query 301 SKWMIERSRNLCLYPNVYLMDQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIR 360
SKWMIERSRNLCLYPNVYLMDQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIR
Sbjct 301 SKWMIERSRNLCLYPNVYLMDQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIR 360
Query 361 QYEDFFNASGMATPDDLEEFRACQAGYAGIELEWNDMCRGSKHWIYGPDDAANEIGLKPA 420
QYEDFFNASGMATPDDLEEFRACQAGYAGIELEWNDMCRGSKHWIYGPDDAANEIGLKPA
Sbjct 361 QYEDFFNASGMATPDDLEEFRACQAGYAGIELEWNDMCRGSKHWIYGPDDAANEIGLKPA 420
Query 421 ISGIKTEDEGLYLAQHQYWLKSMKQAIAAEKEFASRQGENA 461
ISGIKTEDEGLYLAQHQYWLKSMKQAIAAEKEFASRQGENA
Sbjct 421 ISGIKTEDEGLYLAQHQYWLKSMKQAIAAEKEFASRQGENA 461
>P23099 Toluate 1,2-dioxygenase subunit alpha [Pseudomonas putida]
Length=454
Score = 631 bits (1627), Expect = 0.0
Identities = 292/441 (66%), Positives = 351/441 (80%), Gaps = 3/441 (1%)
Query 12 LDRIDELLVDNTETGEFKLHRSVFTDQALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTT 71
LD ID L+ ++ G ++ R +FTD LFDLEMK+IFEGNW+YLAHESQIP NDYYTT
Sbjct 9 LDYIDSLVEEDENEGIYRCKREMFTDPRLFDLEMKHIFEGNWIYLAHESQIPEKNDYYTT 68
Query 72 YIGRQPILIARNRNGELNAMINACSHRGAQLCRHKRGNKTTYTCPFHGWTFNNSGKLLKV 131
+GRQPI I RN++GELNA +NACSHRGA LCR + GNK T+TC FHGWTF+NSGKLLKV
Sbjct 69 QMGRQPIFITRNKDGELNAFVNACSHRGATLCRFRSGNKATHTCSFHGWTFSNSGKLLKV 128
Query 132 KDPSDAGYSDCFNQDGSHDLKKVARFESYKGFLFGSLNPDVPSLQEFLGETTKIIDMIVG 191
KDP AGY D F+ DGSHDLKKVARF SY+GFLFGSL DV L+EFLGE+ K+IDM+V
Sbjct 129 KDPKGAGYPDSFDCDGSHDLKKVARFASYRGFLFGSLREDVAPLEEFLGESRKVIDMVVD 188
Query 192 QSDQGLEVLRGVSTYTYEGNWKLTAENGADGYHVSAVHWNYAATTQHRKEKQAGDTIRAM 251
QS +GLEVLRG STY YEGNWK+ ENGADGYHVS VHWNYAAT Q RK + AGD IRAM
Sbjct 189 QSPEGLEVLRGSSTYVYEGNWKVQVENGADGYHVSTVHWNYAATQQQRKLRDAGDDIRAM 248
Query 252 SAGSWGKHGGGSYGFEHGHMLLWTQWGNPEDRPNFPKAAEYTEKFGAAMSKWMIERSRNL 311
+A SWG GGG Y FE+GH ++W +WG+P++RP F + +FG A + WMI SRNL
Sbjct 249 TASSWGGDGGGFYSFENGHQMVWARWGDPKNRPLFAERDRLASEFGEARADWMIGVSRNL 308
Query 312 CLYPNVYLMDQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIRQYEDFFNASGM 371
CLYPN+YLMDQFGSQ+R+ RP+SV++TE+TIYCIAP GE P RARR+RQYEDFFN SGM
Sbjct 309 CLYPNLYLMDQFGSQLRITRPLSVDRTEITIYCIAPKGETPR-RARRVRQYEDFFNVSGM 367
Query 372 ATPDDLEEFRACQAGYAGIELEWNDMCRGSKHWIYGPDDAANEIGLKPAISGIKTEDEGL 431
ATPDDLEEFRACQ G+AG + NDM RG+KHWI GPD+ A EI L P +SG+++EDEGL
Sbjct 368 ATPDDLEEFRACQEGFAGGGM--NDMSRGAKHWIEGPDEGAKEIDLHPKLSGVRSEDEGL 425
Query 432 YLAQHQYWLKSMKQAIAAEKE 452
++ QH+YW + M +A+ E++
Sbjct 426 FVMQHKYWQQQMIKAVKREQD 446
>Q51601 2-halobenzoate 1,2-dioxygenase large subunit [Burkholderia
cepacia]
Length=465
Score = 545 bits (1403), Expect = 0.0
Identities = 251/437 (57%), Positives = 322/437 (74%), Gaps = 3/437 (1%)
Query 15 IDELLVDNTETGEFKLHRSVFTDQALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIG 74
I + ++ +G F+ R +FTD ALFD EMKYIFE NWV+LAHESQ+ N +DY + IG
Sbjct 18 ISNAVQNDPVSGNFRCRRDIFTDAALFDYEMKYIFEQNWVFLAHESQVANPDDYLVSNIG 77
Query 75 RQPILIARNRNGELNAMINACSHRGAQLCRHKRGNKTTYTCPFHGWTFNNSGKLLKVKDP 134
RQP++I RN+ G+++A+INACSHRGA+LCR K+GN++T+TC FHGWTF+N+GKLLKVKD
Sbjct 78 RQPVIITRNKAGDVSAVINACSHRGAELCRRKQGNRSTFTCQFHGWTFSNTGKLLKVKDG 137
Query 135 SDAGYSDCFNQDGSHDLKKVARFESYKGFLFGSLNPDVPSLQEFLGETTKIIDMIVGQSD 194
D Y + FN DGSHDL ++ F +Y+GFLFGS+NPD ++E LG + I+D ++ Q+
Sbjct 138 QDDNYPEGFNVDGSHDLTRIPSFANYRGFLFGSMNPDACPIEEHLGGSKAILDQVIDQTP 197
Query 195 QGLEVLRGVSTYTYEGNWKLTAENGADGYHVSAVHWNYAATTQHRKEKQAGDTIRAMSAG 254
LEVLRG S+Y Y+GNWKL ENGADGYHV +VHWNY AT R + DTIR +
Sbjct 198 GELEVLRGSSSYIYDGNWKLQIENGADGYHVGSVHWNYVATIGRR--DRTSDTIRTVDVT 255
Query 255 SWGKHG-GGSYGFEHGHMLLWTQWGNPEDRPNFPKAAEYTEKFGAAMSKWMIERSRNLCL 313
+W K GG+Y FEHGHMLLWT+ NPE RP F + E + G ++ ++ ++RNLC+
Sbjct 256 TWSKKNIGGTYTFEHGHMLLWTRLPNPEVRPVFARREELKARVGEEVADAIVNQTRNLCI 315
Query 314 YPNVYLMDQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIRQYEDFFNASGMAT 373
YPN+Y+MDQ +QIRV+RPISV+KTEVTIYC AP E+ E R RIRQYEDFFN SGM T
Sbjct 316 YPNLYVMDQISTQIRVVRPISVDKTEVTIYCFAPRDESEEVRNARIRQYEDFFNVSGMGT 375
Query 374 PDDLEEFRACQAGYAGIELEWNDMCRGSKHWIYGPDDAANEIGLKPAISGIKTEDEGLYL 433
PDDLEEFRACQ+GY G EWND+ RG+ HWI GPDD A +GL P +SG + EDEGL++
Sbjct 376 PDDLEEFRACQSGYRGSAREWNDLSRGAPHWISGPDDNARRLGLAPLMSGARMEDEGLFV 435
Query 434 AQHQYWLKSMKQAIAAE 450
QH YW ++M + I AE
Sbjct 436 QQHTYWAETMLRGIEAE 452
>O85673 Anthranilate 1,2-dioxygenase large subunit [Acinetobacter
baylyi (strain ATCC 33305 / BD413 / ADP1)]
Length=471
Score = 399 bits (1024), Expect = 4e-139
Identities = 197/423 (47%), Positives = 274/423 (65%), Gaps = 13/423 (3%)
Query 26 GEFKLHRSVFTDQALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIGRQPILIARNRN 85
G +++ R +FT+ LF+LEM+ IFE W+Y HES+IPNNND+ T IGRQP++++R+
Sbjct 24 GVYRIARDMFTEPELFELEMELIFEKVWIYACHESEIPNNNDFVTVQIGRQPMIVSRDGK 83
Query 86 GELNAMINACSHRGAQLCRHKRGNKTTYTCPFHGWTFNNSGKLLKVKDPSDAGYSDCFNQ 145
GEL+AM+NAC HRGA L R +GN++ +TCPFH W + + G+L+KVK P + C +
Sbjct 84 GELHAMVNACEHRGATLTRVAKGNQSVFTCPFHAWCYKSDGRLVKVKAPGEY----CEDF 139
Query 146 DGSHDLKKVARFESYKGFLFGSLNPDVP-SLQEFLGETTKIIDMIVGQSDQG-LEVLRGV 203
D S K R SY+GF+F SL+ SL++FLG+ +D++V QS G LEVL+G
Sbjct 140 DKSSRGLKQGRIASYRGFVFVSLDTQATDSLEDFLGDAKVFLDLMVDQSPTGELEVLQGK 199
Query 204 STYTYEGNWKLTAENGADGYHVSAVHWNYAATTQHRKEKQA--GDTIRAMSAGSWG---- 257
S YT+ GNWKL ENG DGYHVS VH+NY +T QHR++ A GD + + G
Sbjct 200 SAYTFAGNWKLQNENGLDGYHVSTVHYNYVSTVQHRQQVNAAKGDELDTLDYSKLGAGDS 259
Query 258 KHGGGSYGFEHGHMLLWTQWGNPEDRPNFPKAAEY-TEKFGAAMSKWMIERSRNLCLYPN 316
+ G + F++GH +L++ NP RP + Y EKFG ++W + R RNL LYP+
Sbjct 260 ETDDGWFSFKNGHSVLFSDMPNPTVRPGYNTVMPYLVEKFGEKRAEWAMHRLRNLNLYPS 319
Query 317 VYLMDQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIRQYEDFFNASGMATPDD 376
++ MDQ SQ+R++RP++ NKTEV CI GE+ EAR RIRQ+EDFFN SG+ TPDD
Sbjct 320 LFFMDQISSQLRIIRPVAWNKTEVISQCIGVKGESSEARRNRIRQFEDFFNVSGLGTPDD 379
Query 377 LEEFRACQAGYAGIELEWNDMCRGSKHWIYGPDDAANEIGLKPAISGIKTEDEGLYLAQH 436
L EFR Q G+ G W+D+ RG W YGP + ++G++P I+G + EGLY+ QH
Sbjct 380 LVEFREQQKGFQGRIERWSDISRGYHQWTYGPTQNSQDLGIEPVITGREFTHEGLYVNQH 439
Query 437 QYW 439
W
Sbjct 440 GQW 442
Score = 22.3 bits (46), Expect = 0.36
Identities = 11/37 (30%), Positives = 18/37 (49%), Gaps = 10/37 (27%)
Query 17 ELLVDNTETGEFKLHRSVFTDQALFDLEMKYIFEGNW 53
+L+VD + TGE ++ + + Y F GNW
Sbjct 182 DLMVDQSPTGELEVLQG----------KSAYTFAGNW 208
>Q51974 p-cumate 2,3-dioxygenase system, large oxygenase component
[Pseudomonas putida]
Length=434
Score = 200 bits (509), Expect = 1e-62
Identities = 110/376 (29%), Positives = 176/376 (47%), Gaps = 5/376 (1%)
Query 28 FKLHRSVFTDQALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIGRQPILIARNRNGE 87
F++ R F + + E + IF+ W+Y+ H S+ D+ T + R+ +L+ +
Sbjct 17 FRVARESFVSEEVLAEEYEKIFDRCWLYVGHTSEFKKPGDFVTRTVARRNLLVTMGTDRT 76
Query 88 LNAMINACSHRGAQLCRHKRGNKTTYTCPFHGWTFNNSGKLLKVKDPSDAGYSDCFNQDG 147
+NA N C HRGA +CR + GN + C +HGW F G L P Y F G
Sbjct 77 INAFFNTCPHRGATVCRERSGNSKNFQCFYHGWVFGCDGNL--KSQPGKERYCADFITGG 134
Query 148 SHDLKKVARFESYKGFLFGSLNPDVPSLQEFLGETTKIIDMIVGQSDQGLEVLRGVSTYT 207
+ +L V RF+ Y GF F S N +V L ++L + ++++ S+ G+ + G Y
Sbjct 135 AGNLVPVPRFDIYAGFCFVSFNAEVEPLPDYLAGAKEYLELVSKYSESGMGITTGTQEYA 194
Query 208 YEGNWKLTAENGADGYHVSAVHWNYAATTQHRKEKQAGDTIRAMSAGSWGKHGGGSYGFE 267
NWKL EN DGYH + H +Y ++ + +G + S H +
Sbjct 195 IRANWKLLVENSIDGYHAVSTHASYLDYLKNINDGFSGAKLEGKSTDLGNGHAVIEFSAP 254
Query 268 HGHMLL-WTQ-WGNPEDRPNFPKAAEYTEKFGAAMSKWMIERSRNLCLYPNVYLMDQFGS 325
G + W WG + A E GA M+ M ++RNL ++PN+ + D
Sbjct 255 WGRPIASWVPIWGEEGKQEIDQIYARLVELHGAEMADRMAYKNRNLLIFPNLIINDIMAI 314
Query 326 QIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIRQYEDFFNASGMATPDDLEEFRACQA 385
+R P + N V + +AP E+ AR R+ + +F G ATPDD+E +CQ
Sbjct 315 TVRTFYPQAPNYMHVNGWSLAPNEESDWARKYRLSNFLEFLGPGGFATPDDVEALESCQN 374
Query 386 GYAGIEL-EWNDMCRG 400
G++ L W+D+ +G
Sbjct 375 GFSNYRLVPWSDISKG 390
>Q53122 Biphenyl 2,3-dioxygenase subunit alpha [Rhodococcus jostii
(strain RHA1)]
Length=460
Score = 165 bits (417), Expect = 4e-49
Identities = 105/372 (28%), Positives = 173/372 (47%), Gaps = 41/372 (11%)
Query 29 KLHRSVFTDQALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIGRQPILIARNRNGEL 88
+L ++TD+AL++ E++ IF +W+ + HE+QIP D+ T Y+G P+++ R +NGE+
Sbjct 32 RLDPRIYTDEALYEQELERIFGRSWLLMGHETQIPKAGDFMTNYMGEDPVMVVRQKNGEI 91
Query 89 NAMINACSHRGAQLCRHKRGNKTTYTCPFHGWTFNNSGKLLKVKDPSDAGYSDCFNQDGS 148
+N C HRG ++CR GN ++TC +HGW ++ G L+ V F +
Sbjct 92 RVFLNQCRHRGMRICRADGGNAKSFTCSYHGWAYDTGGNLVSVP----------FEEQAF 141
Query 149 HDLKK------VARFESYKGFLFGSLNPDVPSLQEFLGETTKIIDMIVGQSDQGLEVLRG 202
L+K AR E+YKG +F + + D P L +LGE +D ++ +++ G E + G
Sbjct 142 PGLRKEDWGPLQARVETYKGLIFANWDADAPDLDTYLGEAKFYMDHMLDRTEAGTEAIPG 201
Query 203 VSTYTYEGNWKLTAEN-GADGYHVSAVHWNYAATTQHRKEKQAG------------DTIR 249
+ + NWK AE +D YH A TT H AG T
Sbjct 202 IQKWVIPCNWKFAAEQFCSDMYH--------AGTTSHLSGILAGLPDGVDLSELAPPTEG 253
Query 250 AMSAGSWGKHGGGSYGFEHGHMLLWTQWGNPEDRPNFPKAAEYTEKFGAAMSKWMIERSR 309
+WG HG G Y + +L E P A + +E+ G+ + ++
Sbjct 254 IQYRATWGGHGSGFYIGDPNLLLAIMGPKVTEYWTQGPAAEKASERLGSTERGQQL-MAQ 312
Query 310 NLCLYPNVYLMDQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIRQYE-DFFNA 368
++ ++P + + IR P N+ EV + + +APE RQ F+A
Sbjct 313 HMTIFPTCSFLPGINT-IRAWHPRGPNEIEVWAFTVVD-ADAPEEMKEEYRQQTLRTFSA 370
Query 369 SGMATPDDLEEF 380
G+ DD E +
Sbjct 371 GGVFEQDDGENW 382
Score = 17.7 bits (34), Expect = 10.0
Identities = 18/79 (23%), Positives = 25/79 (32%), Gaps = 12/79 (15%)
Query 233 AATTQHRKEKQAGDTIRAMSAGSWGKHGGGSYGFE-----HGHMLLWTQWG-------NP 280
A + KE+ T+R SAG + G E GH +
Sbjct 350 ADAPEEMKEEYRQQTLRTFSAGGVFEQDDGENWVEIQQVLRGHKARSRPFNAEMGLGQTD 409
Query 281 EDRPNFPKAAEYTEKFGAA 299
D P++P Y AA
Sbjct 410 SDNPDYPGTISYVYSEEAA 428
>P0C618 Benzene 1,2-dioxygenase subunit alpha [Pseudomonas putida]
Length=450
Score = 164 bits (416), Expect = 4e-49
Identities = 106/371 (29%), Positives = 172/371 (46%), Gaps = 49/371 (13%)
Query 34 VFTDQALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIGRQPILIARNRNGELNAMIN 93
++TD+ L+ LE++ +F +W+ L HE+QI DY TTY+G P+++ R ++ + +N
Sbjct 35 IYTDEDLYQLELERVFARSWLLLGHETQIRKPGDYITTYMGEDPVVVVRQKDASIAVFLN 94
Query 94 ACSHRGAQLCRHKRGNKTTYTCPFHGWTFNNSGKLLKVKDPSDAGYSDCFNQDGSHDLKK 153
C HRG ++CR GN +TC +HGW ++ +G L+ V P +A C N+ LK
Sbjct 95 QCRHRGMRICRADAGNAKAFTCSYHGWAYDTAGNLVNV--PYEAESFACLNKKEWSPLK- 151
Query 154 VARFESYKGFLFGSLNPDVPSLQEFLGETTKIIDMIVGQSDQGLEVLRGVSTYTYEGNWK 213
AR E+YKG +F + + + L +LGE +D ++ +++ G E + GV + NWK
Sbjct 152 -ARVETYKGLIFANWDENAVDLDTYLGEAKFYMDHMLDRTEAGTEAIPGVQKWVIPCNWK 210
Query 214 LTAEN-GADGYHVSAVHWNYAATTQHRKEKQAG------------DTIRAMSAGSWGKHG 260
AE +D YH A TT H AG T+ SWG HG
Sbjct 211 FAAEQFCSDMYH--------AGTTSHLSGILAGLPEDLEMADLAPPTVGKQYRASWGGHG 262
Query 261 GGSYGFEHGHML---------LWTQWGNPEDRPNFPKAAEYTEKFGAAM--SKWMIERSR 309
G Y + ML WT+ P + + E+ G+ SK M+E
Sbjct 263 SGFYVGDPNLMLAIMGPKVTSYWTEG---------PASEKAAERLGSVERGSKLMVE--- 310
Query 310 NLCLYPNVYLMDQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIRQYEDFFNAS 369
++ ++P + + +R P N+ EV + + + + RQ F+A
Sbjct 311 HMTVFPTCSFLPGINT-VRTWHPRGPNEVEVWAFTVVDADAPDDIKEEFRRQTLRTFSAG 369
Query 370 GMATPDDLEEF 380
G+ DD E +
Sbjct 370 GVFEQDDGENW 380
Score = 19.2 bits (38), Expect = 3.3
Identities = 11/33 (33%), Positives = 15/33 (45%), Gaps = 0/33 (0%)
Query 240 KEKQAGDTIRAMSAGSWGKHGGGSYGFEHGHML 272
KE+ T+R SAG + G E H+L
Sbjct 355 KEEFRRQTLRTFSAGGVFEQDDGENWVEIQHIL 387
>A5W4F2 Benzene 1,2-dioxygenase subunit alpha [Pseudomonas putida
(strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)]
Length=450
Score = 164 bits (416), Expect = 4e-49
Identities = 106/371 (29%), Positives = 172/371 (46%), Gaps = 49/371 (13%)
Query 34 VFTDQALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIGRQPILIARNRNGELNAMIN 93
++TD+ L+ LE++ +F +W+ L HE+QI DY TTY+G P+++ R ++ + +N
Sbjct 35 IYTDEDLYQLELERVFARSWLLLGHETQIRKPGDYITTYMGEDPVVVVRQKDASIAVFLN 94
Query 94 ACSHRGAQLCRHKRGNKTTYTCPFHGWTFNNSGKLLKVKDPSDAGYSDCFNQDGSHDLKK 153
C HRG ++CR GN +TC +HGW ++ +G L+ V P +A C N+ LK
Sbjct 95 QCRHRGMRICRADAGNAKAFTCSYHGWAYDTAGNLVNV--PYEAESFACLNKKEWSPLK- 151
Query 154 VARFESYKGFLFGSLNPDVPSLQEFLGETTKIIDMIVGQSDQGLEVLRGVSTYTYEGNWK 213
AR E+YKG +F + + + L +LGE +D ++ +++ G E + GV + NWK
Sbjct 152 -ARVETYKGLIFANWDENAVDLDTYLGEAKFYMDHMLDRTEAGTEAIPGVQKWVIPCNWK 210
Query 214 LTAEN-GADGYHVSAVHWNYAATTQHRKEKQAG------------DTIRAMSAGSWGKHG 260
AE +D YH A TT H AG T+ SWG HG
Sbjct 211 FAAEQFCSDMYH--------AGTTSHLSGILAGLPEDLEMADLAPPTVGKQYRASWGGHG 262
Query 261 GGSYGFEHGHML---------LWTQWGNPEDRPNFPKAAEYTEKFGAAM--SKWMIERSR 309
G Y + ML WT+ P + + E+ G+ SK M+E
Sbjct 263 SGFYVGDPNLMLAIMGPKVTSYWTEG---------PASEKAAERLGSVERGSKLMVE--- 310
Query 310 NLCLYPNVYLMDQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIRQYEDFFNAS 369
++ ++P + + +R P N+ EV + + + + RQ F+A
Sbjct 311 HMTVFPTCSFLPGINT-VRTWHPRGPNEVEVWAFTVVDADAPDDIKEEFRRQTLRTFSAG 369
Query 370 GMATPDDLEEF 380
G+ DD E +
Sbjct 370 GVFEQDDGENW 380
Score = 19.2 bits (38), Expect = 3.3
Identities = 11/33 (33%), Positives = 15/33 (45%), Gaps = 0/33 (0%)
Query 240 KEKQAGDTIRAMSAGSWGKHGGGSYGFEHGHML 272
KE+ T+R SAG + G E H+L
Sbjct 355 KEEFRRQTLRTFSAGGVFEQDDGENWVEIQHIL 387
>Q7N4W0 3-phenylpropionate/cinnamic acid dioxygenase subunit alpha
[Photorhabdus laumondii subsp. laumondii (strain DSM 15139
/ CIP 105565 / TT01)]
Length=453
Score = 164 bits (416), Expect = 5e-49
Identities = 111/388 (29%), Positives = 182/388 (47%), Gaps = 41/388 (11%)
Query 34 VFTDQALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIGRQPILIARNRNGELNAMIN 93
++ D L+ LE++ IF W++LAH+SQIPN D++ TY+G +++ R +NG + A +N
Sbjct 24 IYIDPELYQLELERIFGRCWLFLAHQSQIPNPGDFFNTYMGEDSVVVVRQKNGSVKAFLN 83
Query 94 ACSHRGAQLCRHKRGNKTTYTCPFHGWTFNNSGKLLKVKDPSDA---GYSDCFNQDGSHD 150
C HR ++C GN +TCP+HGW++ G+L+ V P +A + C Q G
Sbjct 84 QCRHRSMRVCYADSGNTRAFTCPYHGWSYGVDGRLIDV--PLEACAYPHGLCKEQWG--- 138
Query 151 LKKVARFESYKGFLFGSLNPDVPSLQEFLGETTKIIDMIVGQSDQGLEVLRGVSTYTYEG 210
L++V E+YKG +FG+ + PSL ++LG+ +D ++ + + G EV+ GV +
Sbjct 139 LQEVPCVENYKGLIFGNWDTTAPSLIDYLGDMAWYLDGVLDRREGGTEVIGGVQKWLINC 198
Query 211 NWKLTAENGA-DGYHVSAVHWNYAATTQHRKEKQAGDTIRAMSAGSWGK----------- 258
NWKL AE A D YH +++A+ Q K GD +A+ A +
Sbjct 199 NWKLPAEQFAGDQYHAL---FSHASAVQVLSVKD-GDDKKALGADQTSRPVWETAKDAVQ 254
Query 259 -----HGGGSYGFEHGHMLLWTQWGNPEDRPNFPKAAEYTEKFGAAMSKWMIERSRNLCL 313
HG G + E +W A Y E + A + R+ L
Sbjct 255 FAQNGHGCGFFLTEKPDANVWVDGA---------VARYYRETYAEAEQRLGKVRALRLAG 305
Query 314 YPNVYLMDQF---GSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIRQYEDFFNASG 370
+ N++ + + +RV P ++ EV +CIA +PE +A F +G
Sbjct 306 HNNIFPTLSWLNGTATMRVWHPRGPDQVEVWAFCIADKAASPEVKAAFENSATRAFGPAG 365
Query 371 MATPDDLEEFRACQAGYAGIELEWNDMC 398
DD E + Q G + + +C
Sbjct 366 FLEQDDSENWVEIQKVLRGYKARNSTLC 393
>O24676 Chlorobenzene dioxygenase subunit alpha [Cupriavidus sp.
(strain PS12)]
Length=449
Score = 161 bits (408), Expect = 6e-48
Identities = 104/371 (28%), Positives = 171/371 (46%), Gaps = 49/371 (13%)
Query 34 VFTDQALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIGRQPILIARNRNGELNAMIN 93
++TD+ L+ +E++ +F +W+ L HE+QI DY T Y+G P+L+ R ++G + +N
Sbjct 35 IYTDEDLYQIELERVFGRSWLLLGHETQIKKPGDYTTNYMGEDPVLVVRQKDGSIAVFLN 94
Query 94 ACSHRGAQLCRHKRGNKTTYTCPFHGWTFNNSGKLLKVKDPSDAGYSDCFNQDGSHDLKK 153
C HRG ++CR GN +TC +HGW ++ +G L+ V P +A C ++ LK
Sbjct 95 QCRHRGMRICRSDAGNAKAFTCSYHGWAYDTAGNLVNV--PFEAESFPCLDKKEWSPLK- 151
Query 154 VARFESYKGFLFGSLNPDVPSLQEFLGETTKIIDMIVGQSDQGLEVLRGVSTYTYEGNWK 213
AR +YKG +F + + D P L +LGE +D ++ +++ G E + GV + NWK
Sbjct 152 -ARVATYKGLIFANWDHDAPDLDTYLGEAKFYMDHMLDRTEAGTEAIPGVQKWVIPCNWK 210
Query 214 LTAENGA-DGYHVSAVHWNYAATTQHRKEKQAG------------DTIRAMSAGSWGKHG 260
L AE D YH AATT H AG T+ WG HG
Sbjct 211 LAAEQFCWDAYH--------AATTAHLSGILAGLPDGVELADLAPPTVGKQYRAPWGGHG 262
Query 261 GGSYGFEHGHML---------LWTQWGNPEDRPNFPKAAEYTEKFGAAM--SKWMIERSR 309
G + E +L WT+ P + + ++ G+ SK +E
Sbjct 263 SGFFIGEPDLLLAIMGPKITSYWTEG---------PASEKAAQRLGSVERGSKLTVE--- 310
Query 310 NLCLYPNVYLMDQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIRQYEDFFNAS 369
++ ++P + + +R P N+ EV + + + + RQ F+A
Sbjct 311 HMTVFPTCSFL-LGANTVRTWHPRGPNEVEVWAFTVVDADAPDDIKEEFRRQTVRTFSAG 369
Query 370 GMATPDDLEEF 380
G+ DD E +
Sbjct 370 GVFEQDDGENW 380
Score = 19.6 bits (39), Expect = 2.5
Identities = 11/33 (33%), Positives = 15/33 (45%), Gaps = 0/33 (0%)
Query 240 KEKQAGDTIRAMSAGSWGKHGGGSYGFEHGHML 272
KE+ T+R SAG + G E H+L
Sbjct 355 KEEFRRQTVRTFSAGGVFEQDDGENWVEIQHVL 387
>Q07944 Benzene 1,2-dioxygenase subunit alpha [Pseudomonas putida]
Length=450
Score = 159 bits (401), Expect = 6e-47
Identities = 102/362 (28%), Positives = 168/362 (46%), Gaps = 31/362 (9%)
Query 34 VFTDQALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIGRQPILIARNRNGELNAMIN 93
++TD+ L+ LE++ +F +W+ L HE+ I DY+TTY+G P+++ R ++ + +N
Sbjct 35 IYTDEDLYQLELERVFARSWLLLGHETHIRKPGDYFTTYMGEDPVVVVRQKDASIAVFLN 94
Query 94 ACSHRGAQLCRHKRGNKTTYTCPFHGWTFNNSGKLLKVKDPSDAGYSDCFNQDGSHDLKK 153
C HRG ++CR GN +TC +HGW ++ +G L+ V P +A C ++ LK
Sbjct 95 QCRHRGMRICRSDAGNAKAFTCSYHGWAYDTAGNLINV--PYEAESFACLDKKEWSPLK- 151
Query 154 VARFESYKGFLFGSLNPDVPSLQEFLGETTKIIDMIVGQSDQGLEVLRGVSTYTYEGNWK 213
AR E+YKG +F + + + L +LGE +D ++ +++ G EV+ G+ + NWK
Sbjct 152 -ARVETYKGLIFANWDENAIDLDTYLGEAKFYMDHMLDRTEAGTEVIPGIQKWVIPCNWK 210
Query 214 LTAEN-GADGYHVSAVHWNYAATTQHRKEKQAG------------DTIRAMSAGSWGKHG 260
AE +D YH A TT H AG SWG HG
Sbjct 211 FAAEQFCSDMYH--------AGTTAHLSGIIAGLPEDLELADLAPPKFGKQYRASWGGHG 262
Query 261 GGSYGFEHGHMLLWTQWGNPEDRPNFPKAAEYTEKFGAAM--SKWMIERSRNLCLYPNVY 318
G Y + ML P A + E+ G+ +K M+E ++ ++P
Sbjct 263 SGFYIGDPNMMLAMMGPKVTSYLTEGPAAEKAAERLGSIERGTKIMLE---HMTVFPTCS 319
Query 319 LMDQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIRQYEDFFNASGMATPDDLE 378
+ + IR P N+ EV + + + + RQ F+A G+ DD E
Sbjct 320 FLPGVNT-IRTWHPRGPNEVEVWAFTVVDADAPDDIKEEFRRQTLRTFSAGGVFEQDDGE 378
Query 379 EF 380
+
Sbjct 379 NW 380
Score = 19.2 bits (38), Expect = 3.3
Identities = 11/33 (33%), Positives = 15/33 (45%), Gaps = 0/33 (0%)
Query 240 KEKQAGDTIRAMSAGSWGKHGGGSYGFEHGHML 272
KE+ T+R SAG + G E H+L
Sbjct 355 KEEFRRQTLRTFSAGGVFEQDDGENWVEIQHIL 387
>P37333 Biphenyl dioxygenase subunit alpha [Paraburkholderia xenovorans
(strain LB400)]
Length=459
Score = 157 bits (396), Expect = 3e-46
Identities = 102/385 (26%), Positives = 173/385 (45%), Gaps = 51/385 (13%)
Query 34 VFTDQALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIGRQPILIARNRNGELNAMIN 93
++ DQ+L++LE++ +F +W+ L HES +P D+ TY+G P+++ R ++ + +N
Sbjct 39 IYADQSLYELELERVFGRSWLLLGHESHVPETGDFLATYMGEDPVVMVRQKDKSIKVFLN 98
Query 94 ACSHRGAQLCRHKRGNKTTYTCPFHGWTFNNSGKLLKVKDPSDAGYSDCFNQDGSHDLKK 153
C HRG ++CR GN +TC +HGW ++ +GKL+ V +A C ++G K
Sbjct 99 QCRHRGMRICRSDAGNAKAFTCSYHGWAYDIAGKLVNVPFEKEA---FCDKKEGDCGFDK 155
Query 154 V------ARFESYKGFLFGSLNPDVPSLQEFLGETTKIIDMIVGQSDQGLEVLRGVSTYT 207
AR +YKG +F + + P L+ +LG+ +D+++ ++ G + G+ +
Sbjct 156 AEWGPLQARVATYKGLVFANWDVQAPDLETYLGDARPYMDVMLDRTPAGTVAIGGMQKWV 215
Query 208 YEGNWKLTAEN-GADGYHVSAVHWNYAATTQHRKEKQAG------------DTIRAMSAG 254
NWK AE +D YH A TT H AG T
Sbjct 216 IPCNWKFAAEQFCSDMYH--------AGTTTHLSGILAGIPPEMDLSQAQIPTKGNQFRA 267
Query 255 SWGKHGGGSYGFEHGHMLLWTQWGNPEDRPNFPKAAEY-TEKFGAAMSKWMIERS----- 308
+WG HG G Y E G +L PK +Y TE A +++ + +
Sbjct 268 AWGGHGSGWYVDEPGSLLAVMG----------PKVTQYWTEGPAAELAEQRLGHTGMPVR 317
Query 309 ----RNLCLYPNVYLMDQFGSQIRVLRPISVNKTEVTIYCIAPVGEAPEARARRIRQYED 364
+++ ++P + F + IR+ P N+ EV + + E + R
Sbjct 318 RMVGQHMTIFPTCSFLPTF-NNIRIWHPRGPNEIEVWAFTLVDADAPAEIKEEYRRHNIR 376
Query 365 FFNASGMATPDDLEEFRACQAGYAG 389
F+A G+ DD E + Q G G
Sbjct 377 NFSAGGVFEQDDGENWVEIQKGLRG 401
Lambda K H a alpha
0.319 0.135 0.426 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 2000672
Database: 785afe96a10ada4ed89566892c84291c.SwissProt.fasta
Posted date: May 9, 2024 12:33 PM
Number of letters in database: 5,456
Number of sequences in database: 12
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
