BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 1a341907e6a2bf610f4b55aa2db553b7.SwissProt.fasta
4 sequences; 1,145 total letters
Query= ACIAD1615
Length=215
Score E
Sequences producing significant alignments: (Bits) Value
P27763 Nitrile hydratase subunit beta [Pseudomonas chlororaphis] 345 2e-126
P13449 Nitrile hydratase subunit beta [Rhodococcus erythropolis] 250 6e-89
P97052 Nitrile hydratase subunit beta [Pseudomonas putida] 108 3e-33
A9V2C1 Probable nitrile hydratase [Monosiga brevicollis] 70.9 2e-18
>P27763 Nitrile hydratase subunit beta [Pseudomonas chlororaphis]
Length=220
Score = 345 bits (886), Expect = 2e-126
Identities = 160/212 (75%), Positives = 182/212 (86%), Gaps = 0/212 (0%)
Query 1 MDGFHDLGGVQGFGAIDHKINSKSYKPVFKKDWEHLAYTLMFLGAGHLNKFNIDQVRHAV 60
MDGFHDLGG QGFG + H INS SYK VFK+DWEHLAY+LMF+G L KF++D+VRHAV
Sbjct 1 MDGFHDLGGFQGFGKVPHTINSLSYKQVFKQDWEHLAYSLMFVGVDQLKKFSVDEVRHAV 60
Query 61 ERIGLRQHVGTEYYERYVIATATLLTEAGVITQQELDEALGSHFQLATPNHSTGRSAIAD 120
ER+ +RQHVGT+YYERY+IATATLL E GVITQ ELD+ALGSHF+LA P H+TGR AI
Sbjct 61 ERLDVRQHVGTQYYERYIIATATLLVETGVITQAELDQALGSHFKLANPAHATGRPAITG 120
Query 121 RPPFEVGDQVTVRDEYVSGHIRVPAYVRGKTGVVVHRTTEKWPFPDAAGHGHTDALPQPT 180
RPPFEVGD+V VRDEYV+GHIR+PAYVRGK GVV+HRT+E+WPFPDA GHG A QPT
Sbjct 121 RPPFEVGDRVVVRDEYVAGHIRMPAYVRGKEGVVLHRTSEQWPFPDAIGHGDLSAAHQPT 180
Query 181 YHVEFNTQELWGDAADAGYVVVDLFESYLDKA 212
YHVEF ++LWGDAAD GYVVVDLFESYLDKA
Sbjct 181 YHVEFRVKDLWGDAADDGYVVVDLFESYLDKA 212
>P13449 Nitrile hydratase subunit beta [Rhodococcus erythropolis]
Length=212
Score = 250 bits (638), Expect = 6e-89
Identities = 118/212 (56%), Positives = 146/212 (69%), Gaps = 1/212 (0%)
Query 1 MDGFHDLGGVQGFGAIDHKINSKSYKPVFKKDWEHLAYTLMFLGAGHLNKFNIDQVRHAV 60
MDG HDL GVQGFG + H +N+ P F +WEHL Y+LMF G L F++D+VR+ V
Sbjct 1 MDGVHDLAGVQGFGKVPHTVNA-DIGPTFHAEWEHLPYSLMFAGVAELGAFSVDEVRYVV 59
Query 61 ERIGLRQHVGTEYYERYVIATATLLTEAGVITQQELDEALGSHFQLATPNHSTGRSAIAD 120
ER+ R ++ T YYERYVI ATL+ E G++TQ EL+ G F L+ P+ S GR A +
Sbjct 60 ERMEPRHYMMTPYYERYVIGVATLMVEKGILTQDELESLAGGPFPLSRPSESEGRPAPVE 119
Query 121 RPPFEVGDQVTVRDEYVSGHIRVPAYVRGKTGVVVHRTTEKWPFPDAAGHGHTDALPQPT 180
FEVG +V VRDEYV GHIR+PAY RG+ G + HRTTEKWPFPDA GHG DA +PT
Sbjct 120 TTTFEVGQRVRVRDEYVPGHIRMPAYCRGRVGTISHRTTEKWPFPDAIGHGRNDAGEEPT 179
Query 181 YHVEFNTQELWGDAADAGYVVVDLFESYLDKA 212
YHV+F +EL+G D G VVVDLFE YL+ A
Sbjct 180 YHVKFAAEELFGSDTDGGSVVVDLFEGYLEPA 211
>P97052 Nitrile hydratase subunit beta [Pseudomonas putida]
Length=217
Score = 108 bits (269), Expect = 3e-33
Identities = 77/224 (34%), Positives = 111/224 (50%), Gaps = 19/224 (8%)
Query 1 MDGFHDLGGVQGFGAIDHKINSKSYKPVFKKDWEHLAYTLMFLGAGHLNKFNIDQVRHAV 60
M+G HD GG G+G + + N +PVF+ DWE +L+ + N FN+D+ RH++
Sbjct 1 MNGIHDTGGAHGYGPVYREPN----EPVFRYDWEKTVMSLLPALLANAN-FNLDEFRHSI 55
Query 61 ERIGLRQHVGTEYYERYVIATATLLTEAGVITQQELDEALGSHFQLA----TPNHSTGRS 116
ER+G ++ YYE ++ LL E GV+T E+ + + A TP S
Sbjct 56 ERMGPAHYLEGTYYEHWLHVFENLLVEKGVLTATEVATGKAASGKTATRVLTPAIVDDSS 115
Query 117 AIADRPP------FEVGDQVTVRDEYVSGHIRVPAYVRGKTGVVVHRTTEKWPFPDAAGH 170
A P F VGD+V V ++ GH R+P Y R K G T + PD A H
Sbjct 116 APGLLRPGGGFSFFPVGDKVRVLNKNPVGHTRMPRYTRAKWG-QWSSTMVCFVTPDTAAH 174
Query 171 GHTDALPQPTYHVEFNTQELWGDAADA--GYVVVDLFESYLDKA 212
G + PQ Y V F + ELWG A + + VDL++ YL+ A
Sbjct 175 GKGEQ-PQHVYTVSFTSVELWGQDASSPKDTIRVDLWDDYLEPA 217
>A9V2C1 Probable nitrile hydratase [Monosiga brevicollis]
Length=496
Score = 70.9 bits (172), Expect = 2e-18
Identities = 60/222 (27%), Positives = 97/222 (44%), Gaps = 32/222 (14%)
Query 2 DGFHDLGGVQGFGAIDHKINSKSYKPVFKKDWEHLAYTLMFLGAGHLNKFNIDQVRHAVE 61
D HD+GG + + + + Y P WE + L+ L + ++D++R VE
Sbjct 7 DLHHDVGGAENMLRLPLDRHERDYLP-----WERHIHALVVLLVKQ-GRMSVDELRRGVE 60
Query 62 RIGLRQHVGTEYYERYVIATATLLTEAGVITQQELDEALGSHFQLATPNHSTGRSAIADR 121
+ YYE++ ++ + +LTE G ++ EL++ L P + +
Sbjct 61 GLPSSLAEQASYYEKWGLSVSRILTEKGTVSGHELEQGF-----LGVPT-----TDLPQV 110
Query 122 PPFEVGDQVTVRD-----EYVSGHIRVPAYVRGKTGVVVHRT-TEKWPFPDAAGHGHTDA 175
P F+VG +V VR Y H+RVP YV G G +V + P A G T
Sbjct 111 PRFQVGQRVMVRPFGTTFAYRQPHLRVPGYVHGAVGTIVELPGLFQDPMTGAYGERGT-- 168
Query 176 LPQPTYHVEFNTQELWGDAADAGY-------VVVDLFESYLD 210
QP Y V F+ + LW + A VVVD+ + +L+
Sbjct 169 -AQPLYRVAFSHRALWPEGAAHAEPGELEDGVVVDVSQPWLE 209
Score = 15.0 bits (27), Expect = 6.3
Identities = 6/24 (25%), Positives = 10/24 (42%), Gaps = 0/24 (0%)
Query 161 KWPFPDAAGHGHTDALPQPTYHVE 184
+W DAA + A+ Y +
Sbjct 342 EWLLTDAAAAAESLAIRTTNYDAD 365
Score = 14.6 bits (26), Expect = 8.3
Identities = 8/29 (28%), Positives = 13/29 (45%), Gaps = 0/29 (0%)
Query 52 NIDQVRHAVERIGLRQHVGTEYYERYVIA 80
N D + ER+G + + E V+A
Sbjct 361 NYDADPASAERVGGHRLFSHNHTELRVVA 389
Lambda K H a alpha
0.322 0.138 0.428 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 184366
Database: 1a341907e6a2bf610f4b55aa2db553b7.SwissProt.fasta
Posted date: May 18, 2024 4:24 PM
Number of letters in database: 1,145
Number of sequences in database: 4
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
