BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 9beb514a7b60427094ebe73dce8c7a5e.SwissProt.fasta
10 sequences; 4,122 total letters
Query= ACIAD1616
Length=200
Score E
Sequences producing significant alignments: (Bits) Value
P27764 Nitrile hydratase subunit alpha [Pseudomonas chlororaphis] 311 8e-113
P13448 Nitrile hydratase subunit alpha [Rhodococcus erythropolis] 259 4e-92
P29378 Low-molecular weight cobalt-containing nitrile hydratase s... 193 2e-66
P97051 Nitrile hydratase subunit alpha [Pseudomonas putida] 189 8e-65
Q53118 Nitrile hydratase subunit alpha [Rhodococcus sp] 189 1e-64
Q7SID2 Cobalt-containing nitrile hydratase subunit alpha [Pseudon... 184 9e-63
P21219 High-molecular weight cobalt-containing nitrile hydratase ... 174 5e-59
O66188 Thiocyanate hydrolase subunit gamma [Thiobacillus thioparus] 140 4e-45
A9V2C1 Probable nitrile hydratase [Monosiga brevicollis] 125 3e-37
A8C752 tRNA (32-2'-O)-methyltransferase regulator THADA [Chloroce... 37.4 2e-06
>P27764 Nitrile hydratase subunit alpha [Pseudomonas chlororaphis]
Length=200
Score = 311 bits (796), Expect = 8e-113
Identities = 148/199 (74%), Positives = 175/199 (88%), Gaps = 1/199 (1%)
Query 1 MSISVSETSS-STPGERAWALHKVLTNKGLIPEGFIEGLTDLLANKFDPANGAQVVAKAW 59
MS S+S T++ STPGERAWAL +VL +K LIPEG++E LT L+A+ + P NGA+VVAKAW
Sbjct 1 MSTSISTTATPSTPGERAWALFQVLKSKELIPEGYVEQLTQLMAHDWSPENGARVVAKAW 60
Query 60 TDPAYRELLLRDGTAACEEFGFTGPQGEYIVALEDTTDVKNVIVCSLCSCTNWPVLGLPP 119
DP +R LLL+DGTAAC +FG+TGPQGEYIVALEDT VKNVIVCSLCSCTNWPVLGLPP
Sbjct 61 VDPQFRALLLKDGTAACAQFGYTGPQGEYIVALEDTPGVKNVIVCSLCSCTNWPVLGLPP 120
Query 120 EWYKSFEFRARLVREGRTVLKELGTELPENMTVKVWDTSAETRYLVLPMRPAGTENMTVE 179
EWYK FEFRARLVREGRTVL+ELGTELP + +KVWDTSAE+RYLVLP RP G+E+M+ E
Sbjct 121 EWYKGFEFRARLVREGRTVLRELGTELPSDTVIKVWDTSAESRYLVLPQRPEGSEHMSEE 180
Query 180 ELQKLITKDVLIGVALPKL 198
+LQ+L+TKDVLIGVALP++
Sbjct 181 QLQQLVTKDVLIGVALPRV 199
>P13448 Nitrile hydratase subunit alpha [Rhodococcus erythropolis]
Length=207
Score = 259 bits (661), Expect = 4e-92
Identities = 117/205 (57%), Positives = 158/205 (77%), Gaps = 6/205 (3%)
Query 1 MSISVSETSSSTP------GERAWALHKVLTNKGLIPEGFIEGLTDLLANKFDPANGAQV 54
MS+++ T+ + +RAWAL + L KGL+P+G++EG F P GA++
Sbjct 1 MSVTIDHTTENAAPAQAPVSDRAWALFRALDGKGLVPDGYVEGWKKTFEEDFSPRRGAEL 60
Query 55 VAKAWTDPAYRELLLRDGTAACEEFGFTGPQGEYIVALEDTTDVKNVIVCSLCSCTNWPV 114
VA+AWTDP +R+LLL DGTAA ++G+ GPQGEYIVA+EDT +KNVIVCSLCSCT WP+
Sbjct 61 VARAWTDPEFRQLLLTDGTAAVAQYGYLGPQGEYIVAVEDTPTLKNVIVCSLCSCTAWPI 120
Query 115 LGLPPEWYKSFEFRARLVREGRTVLKELGTELPENMTVKVWDTSAETRYLVLPMRPAGTE 174
LGLPP WYKSFE+RAR+VRE R VL E+GTE+ ++ ++V+DT+AETRY+VLP RPAGTE
Sbjct 121 LGLPPTWYKSFEYRARVVREPRKVLSEMGTEIASDIEIRVYDTTAETRYMVLPQRPAGTE 180
Query 175 NMTVEELQKLITKDVLIGVALPKLP 199
+ E+LQ+++TKD LIGVA+P++P
Sbjct 181 GWSQEQLQEIVTKDCLIGVAIPQVP 205
>P29378 Low-molecular weight cobalt-containing nitrile hydratase
subunit alpha [Rhodococcus rhodochrous]
Length=207
Score = 193 bits (491), Expect = 2e-66
Identities = 92/184 (50%), Positives = 125/184 (68%), Gaps = 0/184 (0%)
Query 16 RAWALHKVLTNKGLIPEGFIEGLTDLLANKFDPANGAQVVAKAWTDPAYRELLLRDGTAA 75
R A+ +L +KGLI I+ ++ + N+ P GA++VA+AW DP +++ LL D T+A
Sbjct 21 RVKAMEAILVDKGLISTDAIDHMSSVYENEVGPQLGAKIVARAWVDPEFKQRLLTDATSA 80
Query 76 CEEFGFTGPQGEYIVALEDTTDVKNVIVCSLCSCTNWPVLGLPPEWYKSFEFRARLVREG 135
C E G G QGE +V LE+T V N++VC+LCSC WPVLGLPP WYK +RAR VR+
Sbjct 81 CREMGVGGMQGEEMVVLENTGTVHNMVVCTLCSCYPWPVLGLPPNWYKYPAYRARAVRDP 140
Query 136 RTVLKELGTELPENMTVKVWDTSAETRYLVLPMRPAGTENMTVEELQKLITKDVLIGVAL 195
R VL E G ++ +++WD+SAE RY VLP RPAGTEN T E+L L+T+D LIGV++
Sbjct 141 RGVLAEFGYTPDPDVEIRIWDSSAELRYWVLPQRPAGTENFTEEQLADLVTRDSLIGVSV 200
Query 196 PKLP 199
P P
Sbjct 201 PTTP 204
>P97051 Nitrile hydratase subunit alpha [Pseudomonas putida]
Length=210
Score = 189 bits (481), Expect = 8e-65
Identities = 94/184 (51%), Positives = 122/184 (66%), Gaps = 1/184 (1%)
Query 16 RAWALHKVLTNKGLIPEGFIEGLTDLLANKFDPANGAQVVAKAWTDPAYRELLLRDGTAA 75
R AL +L KGL+ ++ + +K P NGA+VVAKAW DPAY+ LL D TAA
Sbjct 24 RVKALESLLIEKGLVDPAAMDLVVQTYEHKVGPRNGAKVVAKAWVDPAYKARLLADATAA 83
Query 76 CEEFGFTGPQGEYIVALEDTTDVKNVIVCSLCSCTNWPVLGLPPEWYKSFEFRARLVREG 135
E GF+G QGE +V LE+T V NV VC+LCSC WP LGLPP WYK+ +R+R+V +
Sbjct 84 IAELGFSGVQGEDMVILENTPAVHNVFVCTLCSCYPWPTLGLPPAWYKAAAYRSRMVSDP 143
Query 136 RTVLKELGTELPENMTVKVWDTSAETRYLVLPMRPAGTENMTVEELQKLITKDVLIGVAL 195
R VL E G +P N ++VWDT+AE RY+VLP RP GTE + E+L +L+T+D +IG L
Sbjct 144 RGVLAEFGLVIPANKEIRVWDTTAELRYMVLPERP-GTEAYSEEQLAELVTRDSMIGTGL 202
Query 196 PKLP 199
P P
Sbjct 203 PTQP 206
>Q53118 Nitrile hydratase subunit alpha [Rhodococcus sp]
Length=199
Score = 189 bits (479), Expect = 1e-64
Identities = 92/185 (50%), Positives = 123/185 (66%), Gaps = 1/185 (1%)
Query 16 RAWALHKVLTNKGLIPEGFIEGLTDLLANKFDPANGAQVVAKAWTDPAYRELLLRDGTAA 75
R AL +L + +I ++ + ++ + P GA+VVAKAWTD ++ LL D T A
Sbjct 14 RVKALESILIEQNVISTAMVDRMVEIYEEEVGPKLGAKVVAKAWTDSEFKARLLDDATEA 73
Query 76 CEEFGFTGPQGEYIVALEDTTDVKNVIVCSLCSCTNWPVLGLPPEWYKSFEFRARLVREG 135
C+E G +G QGE +V LEDT DV + IVC+LCSC WPVLGLPP WYK +RAR+VRE
Sbjct 74 CKELGISGLQGEDMVVLEDTDDVHHAIVCTLCSCYPWPVLGLPPNWYKEPAYRARIVREP 133
Query 136 RTVL-KELGTELPENMTVKVWDTSAETRYLVLPMRPAGTENMTVEELQKLITKDVLIGVA 194
RTVL +E LPE+ +++WDTS+E RY VLP RP GTE + E+L +L+T+D +IGV
Sbjct 134 RTVLSEEFNYHLPESTEIRIWDTSSEMRYWVLPQRPEGTEGWSEEQLAELVTRDSMIGVG 193
Query 195 LPKLP 199
K P
Sbjct 194 PVKTP 198
>Q7SID2 Cobalt-containing nitrile hydratase subunit alpha [Pseudonocardia
thermophila]
Length=204
Score = 184 bits (467), Expect = 9e-63
Identities = 86/179 (48%), Positives = 127/179 (71%), Gaps = 1/179 (1%)
Query 16 RAWALHKVLTNKGLIPEGFIEGLTDLLANKFDPANGAQVVAKAWTDPAYRELLLRDGTAA 75
R AL +L +G++ I+ + ++ N+ P GA+VV KAWTDP +++ LL DGT A
Sbjct 20 RVKALESMLIEQGILTTSMIDRMAEIYENEVGPHLGAKVVVKAWTDPEFKKRLLADGTEA 79
Query 76 CEEFGFTGPQGEYIVALEDTTDVKNVIVCSLCSCTNWPVLGLPPEWYKSFEFRARLVREG 135
C+E G G QGE ++ +E+T +V +V+VC+LCSC WPVLGLPP W+K ++R+R+VRE
Sbjct 80 CKELGIGGLQGEDMMWVENTDEVHHVVVCTLCSCYPWPVLGLPPNWFKEPQYRSRVVREP 139
Query 136 RTVLK-ELGTELPENMTVKVWDTSAETRYLVLPMRPAGTENMTVEELQKLITKDVLIGV 193
R +LK E G E+P + +KVWD+S+E R++VLP RPAGT+ + EEL L+T++ +IGV
Sbjct 140 RQLLKEEFGFEVPPSKEIKVWDSSSEMRFVVLPQRPAGTDGWSEEELATLVTRESMIGV 198
>P21219 High-molecular weight cobalt-containing nitrile hydratase
subunit alpha [Rhodococcus rhodochrous]
Length=203
Score = 174 bits (442), Expect = 5e-59
Identities = 82/180 (46%), Positives = 120/180 (67%), Gaps = 1/180 (1%)
Query 16 RAWALHKVLTNKGLIPEGFIEGLTDLLANKFDPANGAQVVAKAWTDPAYRELLLRDGTAA 75
R A+ +L +GLI ++ + N+ P GA+VVAK+W DP YR+ L D TAA
Sbjct 14 RTKAIETLLYERGLITPAAVDRVVSYYENEIGPMGGAKVVAKSWVDPEYRKWLEEDATAA 73
Query 76 CEEFGFTGPQGEYIVALEDTTDVKNVIVCSLCSCTNWPVLGLPPEWYKSFEFRARLVREG 135
G+ G Q I A+ + + +V+VC+LCSC WPVLGLPP WYKS E+R+R+V +
Sbjct 74 MASLGYAGEQAHQISAVFNDSQTHHVVVCTLCSCYPWPVLGLPPAWYKSMEYRSRVVADP 133
Query 136 RTVLK-ELGTELPENMTVKVWDTSAETRYLVLPMRPAGTENMTVEELQKLITKDVLIGVA 194
R VLK + G ++P+ + V+VWD+S+E RY+V+P RPAGT+ + EEL KL+++D +IGV+
Sbjct 134 RGVLKRDFGFDIPDEVEVRVWDSSSEIRYIVIPERPAGTDGWSEEELTKLVSRDSMIGVS 193
>O66188 Thiocyanate hydrolase subunit gamma [Thiobacillus thioparus]
Length=243
Score = 140 bits (353), Expect = 4e-45
Identities = 69/161 (43%), Positives = 104/161 (65%), Gaps = 11/161 (7%)
Query 48 PANGAQVVAKAWTDPAYRELLLRDGTAACEEFGF---TGPQGEY--------IVALEDTT 96
P A++VAKAW DP Y++L + DG A + G T P ++ + L D+
Sbjct 61 PLPAARLVAKAWLDPEYKKLCIEDGVEASKAVGVNWVTSPPTQFGTPSDYCNLRVLADSP 120
Query 97 DVKNVIVCSLCSCTNWPVLGLPPEWYKSFEFRARLVREGRTVLKELGTELPENMTVKVWD 156
+K+V+VC+LCSC P+LG PEWY+S +R RLVR R VL E G +LP + ++V D
Sbjct 121 TLKHVVVCTLCSCYPRPILGQSPEWYRSPNYRRRLVRWPRQVLAEFGLQLPSEVQIRVAD 180
Query 157 TSAETRYLVLPMRPAGTENMTVEELQKLITKDVLIGVALPK 197
++ +TRY+V+P+RP GT+ T ++L +++T+D LIGVA+PK
Sbjct 181 SNQKTRYIVMPVRPEGTDGWTEDQLAEIVTRDCLIGVAVPK 221
>A9V2C1 Probable nitrile hydratase [Monosiga brevicollis]
Length=496
Score = 125 bits (313), Expect = 3e-37
Identities = 75/202 (37%), Positives = 109/202 (54%), Gaps = 23/202 (11%)
Query 19 ALHKVLTNKGLIPEGFIEGLTDLLANKFDPANGAQVVAKAWTDPAYRELLLRDGTAACEE 78
AL + LT +G++ + L + G Q+VA+AW+D A+ E LL D AA E
Sbjct 295 ALVQTLTRRGVVRSDELHATLASLDALQNSGAGPQLVARAWSDAAFAEWLLTDAAAAAES 354
Query 79 FG---------------------FTGPQGEYIVALEDTTDVKNVIVCSLCSCTNWPVLGL 117
F+ E V + +T V N++ C+LCSC +LGL
Sbjct 355 LAIRTTNYDADPASAERVGGHRLFSHNHTELRV-VANTDTVHNLVCCTLCSCYPTAILGL 413
Query 118 PPEWYKSFEFRARLVREGRTVLKE-LGTELPENMTVKVWDTSAETRYLVLPMRPAGTENM 176
P WYKS FRAR VRE R +L+E G LPE ++V D++A+ RY+VLP RP GTE
Sbjct 414 SPPWYKSKVFRARAVREPRRLLREEFGLVLPEARGIRVHDSTADLRYMVLPQRPQGTEGW 473
Query 177 TVEELQKLITKDVLIGVALPKL 198
+ E L+ ++T+D L+G A+P++
Sbjct 474 SEEHLRTIVTRDSLLGTAVPRV 495
>A8C752 tRNA (32-2'-O)-methyltransferase regulator THADA [Chlorocebus
aethiops]
Length=1953
Score = 37.4 bits (85), Expect = 2e-06
Identities = 26/63 (41%), Positives = 35/63 (56%), Gaps = 8/63 (13%)
Query 117 LPP--EWYKSFEFRARLVREGRTV--LKEL----GTELPENMTVKVWDTSAETRYLVLPM 168
LPP E+ K+ EF ++E RT+ LK L G E + + + VWD+SAE R L LP
Sbjct 1889 LPPAAEFVKTVEFTRLRIQEERTLACLKLLAFLEGKEGEDTLVLSVWDSSAEARQLTLPR 1948
Query 169 RPA 171
A
Sbjct 1949 TEA 1951
Score = 16.9 bits (32), Expect = 5.4
Identities = 7/23 (30%), Positives = 16/23 (70%), Gaps = 3/23 (13%)
Query 165 VLPMRPAGTEN---MTVEELQKL 184
+LPM+P + +TVE+++++
Sbjct 1070 LLPMQPVPESSDGLLTVEQVKEI 1092
Score = 16.2 bits (30), Expect = 9.4
Identities = 5/13 (38%), Positives = 7/13 (54%), Gaps = 0/13 (0%)
Query 109 CTNWPVLGLPPEW 121
C N + LP +W
Sbjct 1126 CPNVSLQKLPEQW 1138
Score = 16.2 bits (30), Expect = 9.4
Identities = 12/49 (24%), Positives = 17/49 (35%), Gaps = 6/49 (12%)
Query 70 RDGTAACEEFGFTGPQGEYIVALEDTTDVKNVIVCSLCSCTNWPVLGLP 118
++ T EF F +AL ++C L N GLP
Sbjct 1758 QENTCQSTEFAFCQVDASIALALA------LAVLCDLLQQWNQLASGLP 1800
Lambda K H a alpha
0.319 0.136 0.416 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 590208
Database: 9beb514a7b60427094ebe73dce8c7a5e.SwissProt.fasta
Posted date: May 18, 2024 8:30 PM
Number of letters in database: 4,122
Number of sequences in database: 10
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
