BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 2f519fcc44e7a6be6f956453dcadc228.SwissProt.fasta
12 sequences; 3,077 total letters
Query= ACIAD1711
Length=242
Score E
Sequences producing significant alignments: (Bits) Value
P20372 Protocatechuate 3,4-dioxygenase beta chain [Acinetobacter ... 506 0.0
P00437 Protocatechuate 3,4-dioxygenase beta chain [Pseudomonas pu... 281 6e-100
P15110 Protocatechuate 3,4-dioxygenase beta chain [Burkholderia c... 209 1e-71
P20371 Protocatechuate 3,4-dioxygenase alpha chain [Acinetobacter... 83.6 3e-23
P00436 Protocatechuate 3,4-dioxygenase alpha chain [Pseudomonas p... 78.2 3e-21
P27098 Chlorocatechol 1,2-dioxygenase [Pseudomonas sp. (strain P51)] 73.9 2e-19
O67987 Chlorocatechol 1,2-dioxygenase [Rhodococcus opacus] 73.6 4e-19
P07773 Catechol 1,2-dioxygenase [Acinetobacter baylyi (strain ATC... 69.3 2e-17
P11451 Chlorocatechol 1,2-dioxygenase [Pseudomonas putida] 63.5 1e-15
O33950 Catechol 1,2-dioxygenase 2 [Acinetobacter lwoffii] 62.8 3e-15
A1IIX3 Hydroxyquinol 1,2-dioxygenase [Rhizobium sp. (strain MTP-1... 58.9 7e-14
P86029 Catechol 1,2-dioxygenase [Candida albicans (strain SC5314 ... 57.4 3e-13
>P20372 Protocatechuate 3,4-dioxygenase beta chain [Acinetobacter
baylyi (strain ATCC 33305 / BD413 / ADP1)]
Length=241
Score = 506 bits (1304), Expect = 0.0
Identities = 241/241 (100%), Positives = 241/241 (100%), Gaps = 0/241 (0%)
Query 1 MSQIIWGAYAQRNTEDHPPAYAPGYKTSVLRSPKNALISIAETLSEVTAPHFSADKFGPK 60
MSQIIWGAYAQRNTEDHPPAYAPGYKTSVLRSPKNALISIAETLSEVTAPHFSADKFGPK
Sbjct 1 MSQIIWGAYAQRNTEDHPPAYAPGYKTSVLRSPKNALISIAETLSEVTAPHFSADKFGPK 60
Query 61 DNDLILNYAKDGLPIGERVIVHGYVRDQFGRPVKNALVEVWQANASGRYRHPNDQYIGAM 120
DNDLILNYAKDGLPIGERVIVHGYVRDQFGRPVKNALVEVWQANASGRYRHPNDQYIGAM
Sbjct 61 DNDLILNYAKDGLPIGERVIVHGYVRDQFGRPVKNALVEVWQANASGRYRHPNDQYIGAM 120
Query 121 DPNFGGCGRMLTDDNGYYVFRTIKPGPYPWRNRINEWRPAHIHFSLIADGWAQRLISQFY 180
DPNFGGCGRMLTDDNGYYVFRTIKPGPYPWRNRINEWRPAHIHFSLIADGWAQRLISQFY
Sbjct 121 DPNFGGCGRMLTDDNGYYVFRTIKPGPYPWRNRINEWRPAHIHFSLIADGWAQRLISQFY 180
Query 181 FEGDTLIDSCPILKTIPSEQQRRALIALEDKSNFIEADSRCYRFDITLRGRRATYFENDL 240
FEGDTLIDSCPILKTIPSEQQRRALIALEDKSNFIEADSRCYRFDITLRGRRATYFENDL
Sbjct 181 FEGDTLIDSCPILKTIPSEQQRRALIALEDKSNFIEADSRCYRFDITLRGRRATYFENDL 240
Query 241 T 241
T
Sbjct 241 T 241
>P00437 Protocatechuate 3,4-dioxygenase beta chain [Pseudomonas
putida]
Length=239
Score = 281 bits (719), Expect = 6e-100
Identities = 133/227 (59%), Positives = 164/227 (72%), Gaps = 0/227 (0%)
Query 12 RNTEDHPPAYAPGYKTSVLRSPKNALISIAETLSEVTAPHFSADKFGPKDNDLILNYAKD 71
R+ HP A P YKTS+ RSP+ AL+SI +++SE T P+FS FG D+DL+LN+
Sbjct 12 RDRNWHPKALTPDYKTSIARSPRQALVSIPQSISETTGPNFSHLGFGAHDHDLLLNFNNG 71
Query 72 GLPIGERVIVHGYVRDQFGRPVKNALVEVWQANASGRYRHPNDQYIGAMDPNFGGCGRML 131
GLPIGER+IV G V DQ+G+PV N LVE+WQANA GRYRH ND+Y+ +DPNFGG GR L
Sbjct 72 GLPIGERIIVAGRVVDQYGKPVPNTLVEMWQANAGGRYRHKNDRYLAPLDPNFGGVGRCL 131
Query 132 TDDNGYYVFRTIKPGPYPWRNRINEWRPAHIHFSLIADGWAQRLISQFYFEGDTLIDSCP 191
TD +GYY FRTIKPGPYPWRN N+WRPAHIHF + A +LI+Q YFEGD LI CP
Sbjct 132 TDSDGYYSFRTIKPGPYPWRNGPNDWRPAHIHFGISGPSIATKLITQLYFEGDPLIPMCP 191
Query 192 ILKTIPSEQQRRALIALEDKSNFIEADSRCYRFDITLRGRRATYFEN 238
I+K+I + + + LIA D +N D YRFDI LRG+R T+FEN
Sbjct 192 IVKSIANPEAVQQLIAKLDMNNANPMDCLAYRFDIVLRGQRKTHFEN 238
>P15110 Protocatechuate 3,4-dioxygenase beta chain [Burkholderia
cepacia]
Length=235
Score = 209 bits (532), Expect = 1e-71
Identities = 109/226 (48%), Positives = 136/226 (60%), Gaps = 1/226 (0%)
Query 12 RNTEDHPPAYAPGYKTSVLRSPKNALISIAETLSEVTAPHFSADKFGPKDNDLILNYAKD 71
R+ HP P Y++SV R P +I + E L + AP + A+ GP D+DL N K+
Sbjct 10 RDWPSHPAYVHPDYRSSVKRGPTRPMIPLKERLRDQYAPVYGAEDLGPLDHDLTKNAVKN 69
Query 72 GLPIGERVIVHGYVRDQFGRPVKNALVEVWQANASGRYRHPNDQYIGAMDPNFGGCGRML 131
G P+GER++V G V D+ G+PV+N LVEVWQANA+GRY H DQ+ +DPNF G GR +
Sbjct 70 GEPLGERIVVTGRVLDEGGKPVRNTLVEVWQANAAGRYVHKVDQHDAPLDPNFLGAGRCM 129
Query 132 TDDNGYYVFRTIKPGPYPWRNRINEWRPAHIHFSLIADGWAQRLISQFYFEGDTLIDSCP 191
TD G Y F TIKPG YPW N N WRP HIHFSL D + RL++Q YF GD L+ P
Sbjct 130 TDAEGRYRFLTIKPGAYPWGNHPNAWRPNHIHFSLFGDYFGSRLVTQMYFPGDPLLAYDP 189
Query 192 ILKTIPSEQQRRALIALEDKSNFIEADSRCYRFDITLRGRRATYFE 237
I + P E R LI+ E + Y FDI LRGR AT E
Sbjct 190 IFQGTP-EAARDRLISRFSLDTTEEGHALGYEFDIVLRGRDATPME 234
>P20371 Protocatechuate 3,4-dioxygenase alpha chain [Acinetobacter
baylyi (strain ATCC 33305 / BD413 / ADP1)]
Length=209
Score = 83.6 bits (205), Expect = 3e-23
Identities = 56/178 (31%), Positives = 91/178 (51%), Gaps = 13/178 (7%)
Query 61 DNDLILNYAKDGLPIGERVIVHGYVRDQFGRPVKNALVEVWQANASGRYRHPNDQYIGAM 120
DN+L+ + + G+R+ + G V D G P+++ L+E+WQA+ +G Y D +
Sbjct 40 DNNLVQDNTQ-----GQRIRLEGQVFDGLGLPLRDVLIEIWQADTNGVYPSQADTQGKQV 94
Query 121 DPNFGGCGRMLTD-DNGYYVFRTIKPGPYPWRNRINEWRPAHIHFSLIADGWAQRLISQF 179
DPNF G GR D G++ F TIKPG P R + HI + A G L ++
Sbjct 95 DPNFLGWGRTGADFGTGFWSFNTIKPGAVPGRK--GSTQAPHISLIIFARGINIGLHTRV 152
Query 180 YFEGDTLIDS-CPILKTIPSEQQRRALIALEDKSNFIEADSRCYRFDITLRGRRATYF 236
YF+ + ++ P+L +I +R+ L+A ++ + YRFDI ++G T F
Sbjct 153 YFDDEAEANAKDPVLNSIEWATRRQTLVAKREERD----GEVVYRFDIRIQGENETVF 206
>P00436 Protocatechuate 3,4-dioxygenase alpha chain [Pseudomonas
putida]
Length=201
Score = 78.2 bits (191), Expect = 3e-21
Identities = 60/180 (33%), Positives = 89/180 (49%), Gaps = 16/180 (9%)
Query 60 KDNDLILNYAKDGLPIGERVIVHGYVRDQFGRPVKNALVEVWQANASGRYRHPNDQYIGA 119
+D ++ AK P GE +++ G V D G V+++ +EVWQA+A+G Y+ D Y
Sbjct 32 RDQEIWNRLAKPDAP-GEHILLLGQVYDGNGHLVRDSFLEVWQADANGEYQ---DAY--N 85
Query 120 MDPNFGGCGRMLTD-DNGYYVFRTIKPGPYPWRNRINEWRPAHIHFSLIADGWAQRLISQ 178
++ F GR T D G + T+KPG N HI+ SL A G L ++
Sbjct 86 LENAFNSFGRTATTFDAGEWTLHTVKPGVV--NNAAGVPMAPHINISLFARGINIHLHTR 143
Query 179 FYFEGDTLIDS-CPILKTIPSEQQRRALIALEDKSNFIEADSR-CYRFDITLRGRRATYF 236
YF+ + ++ CP+L I Q+R LIA E D + YRFDI ++G T F
Sbjct 144 LYFDDEAQANAKCPVLNLIEQPQRRETLIAKR-----CEVDGKTAYRFDIRIQGEGETVF 198
>P27098 Chlorocatechol 1,2-dioxygenase [Pseudomonas sp. (strain
P51)]
Length=251
Score = 73.9 bits (180), Expect = 2e-19
Identities = 41/122 (34%), Positives = 61/122 (50%), Gaps = 17/122 (14%)
Query 79 VIVHGYVRDQFGRPVKNALVEVWQANASGRYRHPNDQYIGAMDPNFGGCGRMLTDDNGYY 138
+++ G VR G P A+++VW + G+Y +DQ P G+++ D G Y
Sbjct 100 LVIRGAVRTDDGAPAAGAVIDVWHSTPDGKYSGIHDQI-----PTDMYRGKVVADAQGKY 154
Query 139 VFRTIKPGPYPWRNR-----------INEWRPAHIHFSLIADGWAQRLISQFYFEGDTLI 187
RT P PY N+ + WRPAH+HF + DG+A L +Q+YFEG +
Sbjct 155 AVRTTMPAPYQIPNKGPTGVLLEMMGSHTWRPAHVHFKVRKDGFAP-LTTQYYFEGGDWV 213
Query 188 DS 189
DS
Sbjct 214 DS 215
>O67987 Chlorocatechol 1,2-dioxygenase [Rhodococcus opacus]
Length=257
Score = 73.6 bits (179), Expect = 4e-19
Identities = 46/134 (34%), Positives = 70/134 (52%), Gaps = 19/134 (14%)
Query 76 GERVIVHGYVRDQFGRPVKNALVEVWQANASGRYRHPNDQYIGAMDPNFGGCGRMLTDDN 135
G+R+ G VRD G P+ A+++VW + G Y + A+ + GR++ ++
Sbjct 101 GDRMRFTGSVRDTSGTPITGAVIDVWHSTNDGNYSF----FSPALPDQYLLRGRVVPAED 156
Query 136 GYYVFRTIKPGPY------PWRNRIN------EWRPAHIHFSLIADGWAQRLISQFYFEG 183
G F +I+P PY P +N WRPAHIH + ADG+ + LI+Q YFEG
Sbjct 157 GSIEFHSIRPVPYEIPKAGPTGQLMNSYLGRHSWRPAHIHIRITADGY-RPLITQLYFEG 215
Query 184 DTLI--DSCPILKT 195
D + DSC +K+
Sbjct 216 DPYLDSDSCSAVKS 229
>P07773 Catechol 1,2-dioxygenase [Acinetobacter baylyi (strain
ATCC 33305 / BD413 / ADP1)]
Length=311
Score = 69.3 bits (168), Expect = 2e-17
Identities = 46/127 (36%), Positives = 63/127 (50%), Gaps = 25/127 (20%)
Query 74 PIGERVIVHGYVRDQFGRPVKNALVEVWQANASGRYRHPNDQYIGAMDPN-----FGGCG 128
P G +I+HG + D G+P+ NA VE+W AN G Y H DP F
Sbjct 129 PNGHTLILHGTIFDADGKPLPNAKVEIWHANTKGFYSH--------FDPTGEQQAFNMRR 180
Query 129 RMLTDDNGYYVFRTIKPGPY------PWRNRINEW-----RPAHIHFSLIADGWAQRLIS 177
++TD+NG Y RTI P Y P + +N+ RPAHIH+ + ADG ++L +
Sbjct 181 SIITDENGQYRVRTILPAGYGCPPEGPTQQLLNQLGRHGNRPAHIHYFVSADG-HRKLTT 239
Query 178 QFYFEGD 184
Q GD
Sbjct 240 QINVAGD 246
>P11451 Chlorocatechol 1,2-dioxygenase [Pseudomonas putida]
Length=260
Score = 63.5 bits (153), Expect = 1e-15
Identities = 39/121 (32%), Positives = 58/121 (48%), Gaps = 17/121 (14%)
Query 79 VIVHGYVRDQFGRPVKNALVEVWQANASGRYRHPNDQYIGAMDPNFGGCGRMLTDDNGYY 138
+I+ G VR G + A+++VW + G Y +D P G+++TD G Y
Sbjct 100 LIIRGTVRSDTGELLAGAVIDVWHSTPDGLYSGIHDNI-----PVDYYRGKLVTDSQGNY 154
Query 139 VFRTIKPGPY--PWRNRINE---------WRPAHIHFSLIADGWAQRLISQFYFEGDTLI 187
RT P PY P+ WRPAH+HF + DG+ + L +Q+YFEG +
Sbjct 155 RVRTTMPVPYQIPYEGPTGRLLGHLGSHTWRPAHVHFKVRKDGF-EPLTTQYYFEGGKWV 213
Query 188 D 188
D
Sbjct 214 D 214
>O33950 Catechol 1,2-dioxygenase 2 [Acinetobacter lwoffii]
Length=275
Score = 62.8 bits (151), Expect = 3e-15
Identities = 43/125 (34%), Positives = 62/125 (50%), Gaps = 17/125 (14%)
Query 71 DGLPIGERVIVHGYVRDQFGRPVKNALVEVWQANASGRYRHPNDQYIGAMDPNFGGCGRM 130
DG G+ +++ G V + G+P+ NALVEVW AN G Y Y P F +
Sbjct 120 DGTDPGQTLVMRGRVFGEDGKPLANALVEVWHANHLGNY-----SYFDKSQPAFNLRRSI 174
Query 131 LTDDNGYYVFRTIKPGPY--PWRNRI---------NEWRPAHIHFSLIADGWAQRLISQF 179
TD G Y FR++ P Y P + + + RPAHIHF + A G+ ++L +Q
Sbjct 175 RTDAEGKYSFRSVVPVGYSVPPQGQTQLLLDQLGRHGHRPAHIHFFVSAPGF-RKLTTQI 233
Query 180 YFEGD 184
+GD
Sbjct 234 NIDGD 238
>A1IIX3 Hydroxyquinol 1,2-dioxygenase [Rhizobium sp. (strain MTP-10005)]
Length=295
Score = 58.9 bits (141), Expect = 7e-14
Identities = 40/125 (32%), Positives = 61/125 (49%), Gaps = 16/125 (13%)
Query 76 GERVIVHGYVRDQFGRPVKNALVEVWQANASGRYRHPNDQYIGAMDPNFGGCGRMLTDDN 135
GE ++V G + D G PV A ++VWQAN G Y D + P+F G +T ++
Sbjct 132 GEDMLVTGRILDTDGVPVAGARIDVWQANDEGFY----DVQQKGIQPDFNLRGVFVTGED 187
Query 136 GYYVFRTIKPGPYPWRN-----------RINEWRPAHIHFSLIADGWAQRLISQFYFEGD 184
G Y FR KP YP + + +RPAH+H+ + A+G+ L++ + D
Sbjct 188 GRYWFRAAKPKYYPIPDDGPVGQLLRAMGRHPYRPAHLHYIVSAEGFT-TLVTHIFDPDD 246
Query 185 TLIDS 189
I S
Sbjct 247 PYIRS 251
Score = 16.5 bits (31), Expect = 6.0
Identities = 5/8 (63%), Positives = 7/8 (88%), Gaps = 0/8 (0%)
Query 132 TDDNGYYV 139
T D+GY+V
Sbjct 6 TGDDGYFV 13
>P86029 Catechol 1,2-dioxygenase [Candida albicans (strain SC5314
/ ATCC MYA-2876)]
Length=303
Score = 57.4 bits (137), Expect = 3e-13
Identities = 38/119 (32%), Positives = 55/119 (46%), Gaps = 16/119 (13%)
Query 73 LPIGERVIVHGYVRDQFGRPVKNALVEVWQANASGRYRHPNDQYIGAMDPNFGGCGRMLT 132
+P + V G V D G+P+ A +EVWQ N++G Y D P F G +T
Sbjct 120 IPTDVKCFVRGKVTDTEGKPLGGAQLEVWQCNSAGFYSQQADH----DGPEFNLRGTFIT 175
Query 133 DDNGYYVFRTIKPG--PYPWRNRINEW---------RPAHIHFSLIADGWAQRLISQFY 180
DD G Y F ++P P P+ + RP+HIH+ + G+ LI+Q Y
Sbjct 176 DDEGNYSFECLRPTSYPIPYDGPAGDLLKIMDRHPNRPSHIHWRVSHPGY-HTLITQIY 233
Score = 16.2 bits (30), Expect = 8.0
Identities = 12/66 (18%), Positives = 25/66 (38%), Gaps = 12/66 (18%)
Query 5 IWGAYAQRNTEDHPPAYAPGYKTSVLRSPKNALISIAETLSEVTAPHFSADKFGPKDNDL 64
I + R + H PGY T + + + + P+ + D +D+
Sbjct 205 IMDRHPNRPSHIHWRVSHPGYHTLITQ------------IYDAECPYTNNDSVYAVKDDI 252
Query 65 ILNYAK 70
I+++ K
Sbjct 253 IVHFEK 258
Lambda K H a alpha
0.323 0.140 0.448 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 524594
Database: 2f519fcc44e7a6be6f956453dcadc228.SwissProt.fasta
Posted date: May 20, 2024 12:15 AM
Number of letters in database: 3,077
Number of sequences in database: 12
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
