BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: b9d1fb4b793bb1b900e9e571b26464e5.SwissProt.fasta
9 sequences; 3,175 total letters
Query= ACIAD1800
Length=285
Score E
Sequences producing significant alignments: (Bits) Value
P37327 Inner membrane protein YfdC [Escherichia coli (strain K12)] 190 2e-62
P38750 Uncharacterized transporter YHL008C [Saccharomyces cerevis... 61.6 3e-14
P77733 Formate channel FocB [Escherichia coli (strain K12)] 57.0 5e-13
P0AC24 Formate channel FocA [Escherichia coli O6:H1 (strain CFT07... 53.5 8e-12
P0AC23 Formate channel FocA [Escherichia coli (strain K12)] 53.5 8e-12
P0AC25 Formate channel FocA [Escherichia coli O157:H7] 53.5 8e-12
Q50568 Probable formate transporter [Methanothermobacter thermaut... 44.3 8e-09
P35839 Probable formate transporter [Methanobacterium formicicum] 38.1 9e-07
Q12301 Uncharacterized membrane protein YDL180W [Saccharomyces ce... 35.8 7e-06
>P37327 Inner membrane protein YfdC [Escherichia coli (strain
K12)]
Length=310
Score = 190 bits (482), Expect = 2e-62
Identities = 106/285 (37%), Positives = 166/285 (58%), Gaps = 4/285 (1%)
Query 1 MSKTERDKIKVEMEIDPHLSWQEKLAVEEQEKLSPRL--VYEIIRRDGVEELSRPTSTLI 58
M + D+ E+E++ + K ++++L R ++E IR+DG +EL R L+
Sbjct 1 MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHIRQDGEKELERDAMALL 60
Query 59 FSGITAGLVMSFSFVFKAVIASYLPIDAVWTDLITNIGYTIGFLIAILGHLQLFTENTIT 118
+S I AGL M S + K + L + L+ N+GYT GF+I I+ QLFTENT+T
Sbjct 61 WSAIAAGLSMGASLLAKGIFQVELE-GVPGSFLLENLGYTFGFIIVIMARQQLFTENTVT 119
Query 119 TVIPLFKPFSLDKLKAVARLWSIVILTNIFGTALSSLFFLTTDLFSPEVDHALDEIAHHV 178
V+P+ + ++ + + RLW +V+L NI GT +++ F +F+ E A +I V
Sbjct 120 AVLPVMQKPTMSNVGLLIRLWGVVLLGNILGTGIAAWAFEYMPIFNEETRDAFVKIGMDV 179
Query 179 ASFTAWENFLKGVLSGLLIAALVWMLPSVN-NKILLIFMITYLIGLGDFTHVVVGSVEVA 237
T E F ++SG LIA +VWM P+ KI++I ++T+LI LGD TH+VVGSVE+
Sbjct 180 MKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIILMTWLIALGDTTHIVVGSVEIL 239
Query 238 YLVWQGQTSLSEYFFQFLVPTTLGNIIGGTGIFTLLIYGQVNQEL 282
YLV+ G S++ + F +PT GNI GGT IF L+ + Q+ ++
Sbjct 240 YLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMSHAQIRNDM 284
>P38750 Uncharacterized transporter YHL008C [Saccharomyces cerevisiae
(strain ATCC 204508 / S288c)]
Length=627
Score = 61.6 bits (148), Expect = 3e-14
Identities = 63/258 (24%), Positives = 115/258 (45%), Gaps = 28/258 (11%)
Query 27 VEEQEKLSPRLVYEIIRRDGVEELSRPTSTLIFSGITAGLVMSF-SFVFKAVIASYLPID 85
V++ L+P + +++ TL+ + I G++ S SF+ AV + I
Sbjct 2 VDDSNYLTPHETALAVVATAMKKARLQLDTLLINSILGGVLFSSGSFLLVAVYSEDPDIV 61
Query 86 AV---WTDLITNIGYTIGFLIAILGHLQLFTENTITTVIPLFKPFSLDKLKAVARLWSIV 142
A +LIT + + +G ++ LF N + FS+ L+ ++ ++
Sbjct 62 ARNPGIVNLITGVNFAMGLFYVVMMGADLFNSNILF--------FSVGVLRKAVTIYDLM 113
Query 143 I------LTNIFGTALSSLFFLTTDLFSPEVDHALD--EIAHHVASFTAWENFLKGVLSG 194
I L NI G+ S F S + + +I S++ + FLKG+
Sbjct 114 ISWVVSWLGNIAGSLFVSYLFGHLSGISSQKLWIIGSRQIIEQKVSYSFVQTFLKGIACN 173
Query 195 LLIAALVW---MLPSVNNK-ILLIFMITYLIGLGDFTHVVVGSVEVAYLVWQG--QTSLS 248
+ ++ M ++ K IL+ F I IG+G FTHVV G + +++ S+
Sbjct 174 FFVCLAIYLQLMAKPIHVKFILMSFPIIDFIGIG-FTHVV-GDMSASFIAMLNGANVSVG 231
Query 249 EYFFQFLVPTTLGNIIGG 266
+Y ++ L+P +LGNI+GG
Sbjct 232 KYIWKLLIPASLGNIVGG 249
>P77733 Formate channel FocB [Escherichia coli (strain K12)]
Length=282
Score = 57.0 bits (136), Expect = 5e-13
Identities = 59/267 (22%), Positives = 106/267 (40%), Gaps = 48/267 (18%)
Query 41 IIRRDGVEELSRPTSTLIFSGITAGLVMSFSFVFKAVIASYLPIDAVWTDLITNIGYTIG 100
I R +++R ++ ++AG+ M+ F F + + P T L+ + +T+G
Sbjct 18 IAERIAAHKIARSKVSVFLMAMSAGVFMAIGFTFYLSVIADAPSSQALTHLVGGLCFTLG 77
Query 101 FLIAILGHLQLFTENTITTVIPLFKPFSLDKLKAVA--RLWSI----VILTNIFGTALSS 154
F++ + LFT + +T + K + V R W I V N+ G A S
Sbjct 78 FILLAVCGTSLFTSSVMTV---------MAKSRGVISWRTWLINALLVACGNLAGIACFS 128
Query 155 LFFLTTDLFSPE-------VDHALDEIAHHVASFTAWENFLKGVLSGLLIAALVWML--- 204
L + L E V H + HH T E+ G++ L++ +WM
Sbjct 129 LLIWFSGLVMSENAMWGVAVLHCAEGKMHH----TFTESVSLGIMCNLMVCLALWMSYCG 184
Query 205 PSVNNKILLIFMITYLIGLGDFTHVVVGSVEVAYLV----------WQ---------GQT 245
S+ +KI+ + + L F H + + + + WQ
Sbjct 185 RSLCDKIVAMILPITLFVASGFEHCIANLFVIPFAIAIRHFAPPPFWQLAHSSADNFPAL 244
Query 246 SLSEYFFQFLVPTTLGNIIGGTGIFTL 272
++S + L+P LGNIIGG + ++
Sbjct 245 TVSHFITANLLPVMLGNIIGGAVLVSM 271
>P0AC24 Formate channel FocA [Escherichia coli O6:H1 (strain CFT073
/ ATCC 700928 / UPEC)]
Length=285
Score = 53.5 bits (127), Expect = 8e-12
Identities = 61/272 (22%), Positives = 109/272 (40%), Gaps = 36/272 (13%)
Query 33 LSPRLVYEIIRRDGVEELSRPTSTLIFSGITAGLVMSFSFVFKAVIAS---YLPIDAVWT 89
L P + ++ GV + ++ + ITAG+ +S +FVF + +P
Sbjct 10 LLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGTMPFG--MA 67
Query 90 DLITNIGYTIGFLIAILGHLQLFTENTITTVIPLFKPFSLDKLKAVARLWSIVILTNIFG 149
L+ I +++G ++ ++ LFT T +I + K +A+ W V N+ G
Sbjct 68 KLVGGICFSLGLILCVVCGADLFTS---TVLIVVAKASGRITWGQLAKNWLNVYFGNLVG 124
Query 150 TAL-SSLFFLTTDLFSPEVDHALD--EIAHHVASFTAWENFLKGVLSGLLIAALVWMLPS 206
L L +L+ + + L+ + A H T E G+L+ L++ VWM S
Sbjct 125 ALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHTFIEAVCLGILANLMVCLAVWMSYS 184
Query 207 ----VNNKILLIFMITYLIGLGDFTHVVVGSVEVAYLV----------WQGQTSLSEYFF 252
++ +++ + + G F H + + + W S E F
Sbjct 185 GRSLMDKAFIMVLPVAMFVASG-FEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPENFS 243
Query 253 QF---------LVPTTLGNIIGGTGIFTLLIY 275
L+P T+GNIIGG G+ L Y
Sbjct 244 HLTVMNFITDNLIPVTIGNIIGG-GLLVGLTY 274
Score = 16.2 bits (30), Expect = 9.9
Identities = 4/19 (21%), Positives = 9/19 (47%), Gaps = 0/19 (0%)
Query 252 FQFLVPTTLGNIIGGTGIF 270
F L+P + + G++
Sbjct 7 FDLLLPAAMAKVAEEAGVY 25
>P0AC23 Formate channel FocA [Escherichia coli (strain K12)]
Length=285
Score = 53.5 bits (127), Expect = 8e-12
Identities = 61/272 (22%), Positives = 109/272 (40%), Gaps = 36/272 (13%)
Query 33 LSPRLVYEIIRRDGVEELSRPTSTLIFSGITAGLVMSFSFVFKAVIAS---YLPIDAVWT 89
L P + ++ GV + ++ + ITAG+ +S +FVF + +P
Sbjct 10 LLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGTMPFG--MA 67
Query 90 DLITNIGYTIGFLIAILGHLQLFTENTITTVIPLFKPFSLDKLKAVARLWSIVILTNIFG 149
L+ I +++G ++ ++ LFT T +I + K +A+ W V N+ G
Sbjct 68 KLVGGICFSLGLILCVVCGADLFTS---TVLIVVAKASGRITWGQLAKNWLNVYFGNLVG 124
Query 150 TAL-SSLFFLTTDLFSPEVDHALD--EIAHHVASFTAWENFLKGVLSGLLIAALVWMLPS 206
L L +L+ + + L+ + A H T E G+L+ L++ VWM S
Sbjct 125 ALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHTFIEAVCLGILANLMVCLAVWMSYS 184
Query 207 ----VNNKILLIFMITYLIGLGDFTHVVVGSVEVAYLV----------WQGQTSLSEYFF 252
++ +++ + + G F H + + + W S E F
Sbjct 185 GRSLMDKAFIMVLPVAMFVASG-FEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPENFS 243
Query 253 QF---------LVPTTLGNIIGGTGIFTLLIY 275
L+P T+GNIIGG G+ L Y
Sbjct 244 HLTVMNFITDNLIPVTIGNIIGG-GLLVGLTY 274
Score = 16.2 bits (30), Expect = 9.9
Identities = 4/19 (21%), Positives = 9/19 (47%), Gaps = 0/19 (0%)
Query 252 FQFLVPTTLGNIIGGTGIF 270
F L+P + + G++
Sbjct 7 FDLLLPAAMAKVAEEAGVY 25
>P0AC25 Formate channel FocA [Escherichia coli O157:H7]
Length=285
Score = 53.5 bits (127), Expect = 8e-12
Identities = 61/272 (22%), Positives = 109/272 (40%), Gaps = 36/272 (13%)
Query 33 LSPRLVYEIIRRDGVEELSRPTSTLIFSGITAGLVMSFSFVFKAVIAS---YLPIDAVWT 89
L P + ++ GV + ++ + ITAG+ +S +FVF + +P
Sbjct 10 LLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTGTMPFG--MA 67
Query 90 DLITNIGYTIGFLIAILGHLQLFTENTITTVIPLFKPFSLDKLKAVARLWSIVILTNIFG 149
L+ I +++G ++ ++ LFT T +I + K +A+ W V N+ G
Sbjct 68 KLVGGICFSLGLILCVVCGADLFTS---TVLIVVAKASGRITWGQLAKNWLNVYFGNLVG 124
Query 150 TAL-SSLFFLTTDLFSPEVDHALD--EIAHHVASFTAWENFLKGVLSGLLIAALVWMLPS 206
L L +L+ + + L+ + A H T E G+L+ L++ VWM S
Sbjct 125 ALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHTFIEAVCLGILANLMVCLAVWMSYS 184
Query 207 ----VNNKILLIFMITYLIGLGDFTHVVVGSVEVAYLV----------WQGQTSLSEYFF 252
++ +++ + + G F H + + + W S E F
Sbjct 185 GRSLMDKAFIMVLPVAMFVASG-FEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPENFS 243
Query 253 QF---------LVPTTLGNIIGGTGIFTLLIY 275
L+P T+GNIIGG G+ L Y
Sbjct 244 HLTVMNFITDNLIPVTIGNIIGG-GLLVGLTY 274
Score = 16.2 bits (30), Expect = 9.9
Identities = 4/19 (21%), Positives = 9/19 (47%), Gaps = 0/19 (0%)
Query 252 FQFLVPTTLGNIIGGTGIF 270
F L+P + + G++
Sbjct 7 FDLLLPAAMAKVAEEAGVY 25
>Q50568 Probable formate transporter [Methanothermobacter thermautotrophicus]
Length=274
Score = 44.3 bits (103), Expect = 8e-09
Identities = 57/249 (23%), Positives = 99/249 (40%), Gaps = 33/249 (13%)
Query 53 PTSTLIFSGITAGLVMSFSFVFKAVIASYLP---IDAVWTDLITNIGYTIGFLIAILGHL 109
P +I AG ++F + V+ + A L+ + +G ++ ++
Sbjct 26 PLGKVIVLSFLAGAYIAFGGLLAEVVTGGMAKAGYPAGLVKLVFGAVFPVGLMLVVIAGS 85
Query 110 QLFTENTITTVIPLFKPFSLDK---LKAVARLWSIVILTNIFGTALSSLFF------LTT 160
+LFT N + + + LDK + + R W + N+ G + F LT
Sbjct 86 ELFTGNCMYMPLGI-----LDKRASIMGLIRNWVTSWVFNLVGAVFVAYFLAVATGILTA 140
Query 161 DLFSPEVDHALDEIAHHVASFTA---------W-ENFLKGVLSGLLIAALVWMLPSVNNK 210
D + A ASF A W + F + V L+ V++ + ++
Sbjct 141 DPWQAGALTVAKTKALGGASFIAAGKVTKSLTWAQAFWRAVGCNWLVCLAVYLAIASDDI 200
Query 211 ILLI----FMITYLIGLGDFTHVVVGSVEVAYLVWQGQTSLSEYFFQFLVPTTLGNIIGG 266
I I F I + +G F H V + ++ G + S++F L+P TLGNI+GG
Sbjct 201 IGKIWGIWFPIFAFVAIG-FEHSVANMFFIPVGIFLGGVTWSQFFMNNLIPVTLGNIVGG 259
Query 267 TGIFTLLIY 275
IF +Y
Sbjct 260 -AIFVACLY 267
>P35839 Probable formate transporter [Methanobacterium formicicum]
Length=280
Score = 38.1 bits (87), Expect = 9e-07
Identities = 51/246 (21%), Positives = 97/246 (39%), Gaps = 27/246 (11%)
Query 53 PTSTLIFSGITAGLVMSFSFVFKAVIASYLPIDAVWTDLITNIG---YTIGFLIAILGHL 109
P S LI AG ++F + V + T L+ + + +G ++ ++
Sbjct 26 PLSNLIVLSFVAGAYIAFGGLLAEVATGGMAAAGWPTGLVKLVFGGVFPVGLMLVVIAGS 85
Query 110 QLFTENTITTVIPLFKPFSLDKLKAVARLWSIVILTNIFGTALSS--LFFLTTDLFSPEV 167
+LFT N + + + + + + + W + N+ G + L +LT L +
Sbjct 86 ELFTGNCMYMPMGILQGEA--SVMGTIKNWVGSWVFNLVGALFVAYVLAYLTGILTAEPW 143
Query 168 DHALDEIAHH--------------VASFTAWENFLKGVLSGLLIAALVWMLPS----VNN 209
IA V S + + F + + L+ V++ + +
Sbjct 144 AATAVTIAKTKALGGAQFIAAGKTVTSLSWMQVFWRAIGCNWLVCLAVYLAVASDDVIGK 203
Query 210 KILLIFMITYLIGLGDFTHVVVGSVEVAYLVWQGQTSLSEYFFQFLVPTTLGNIIGGTGI 269
+ F I + +G F HVV + ++ G + S++F ++P TLGNI+GG I
Sbjct 204 SFGIWFPIMAFVCIG-FEHVVANMFFIPVGIFIGGVTWSQFFINNMIPATLGNIVGG-AI 261
Query 270 FTLLIY 275
F IY
Sbjct 262 FVGCIY 267
Score = 18.5 bits (36), Expect = 1.8
Identities = 12/48 (25%), Positives = 21/48 (44%), Gaps = 11/48 (23%)
Query 194 GLLIAALVWMLPSVNNKILLIFMITYLIGLGDFTHVVVGSVEVAYLVW 241
G+ I + W +NN MI +G ++V G++ V + W
Sbjct 232 GIFIGGVTWSQFFINN------MIPATLG-----NIVGGAIFVGCIYW 268
>Q12301 Uncharacterized membrane protein YDL180W [Saccharomyces
cerevisiae (strain ATCC 204508 / S288c)]
Length=547
Score = 35.8 bits (81), Expect = 7e-06
Identities = 28/94 (30%), Positives = 45/94 (48%), Gaps = 14/94 (15%)
Query 141 IVILTNIFGTALSSLFFLTTDLFSPEVDHALDEIAHHVASFTAWENFLKGVLSGLLIAAL 200
IVIL+ +F +L SLF+ +++L + D D + S + FL G+ S L L
Sbjct 22 IVILSALFSISLFSLFYASSELLLHQYD---DPLMFKPNSQDYFRTFLLGLFSPFLYYFL 78
Query 201 VWMLPSVNNKILL-----------IFMITYLIGL 223
L ++N + L+ +FM+ LIGL
Sbjct 79 KTFLFNINQRFLILNLIVDFPINDVFMLLILIGL 112
Score = 21.6 bits (44), Expect = 0.21
Identities = 13/49 (27%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Query 68 MSFSFVFKAVIASYLPIDAVWTDLITNIGYTI---GFLIAILGHLQLFT 113
++FS ++ V L I V ++++ IG ++ + + + GH LFT
Sbjct 333 IAFSTAYQLVTGLLLMILVVGSNIMLTIGESLILSMYFVYVRGHEGLFT 381
Lambda K H a alpha
0.326 0.142 0.420 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 679671
Database: b9d1fb4b793bb1b900e9e571b26464e5.SwissProt.fasta
Posted date: May 19, 2024 9:23 PM
Number of letters in database: 3,175
Number of sequences in database: 9
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
