BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 66948fe2db9f26d7a0181436751a139e.SwissProt.fasta
6 sequences; 2,586 total letters
Query= ACIAD1869
Length=427
Score E
Sequences producing significant alignments: (Bits) Value
P0AAT5 Miniconductance mechanosensitive channel YbdG [Shigella fl... 310 3e-106
P0AAT4 Miniconductance mechanosensitive channel YbdG [Escherichia... 310 3e-106
Q58543 Large-conductance mechanosensitive channel MscMJLR [Methan... 57.0 2e-12
P0AEB6 Low conductance mechanosensitive channel YnaI [Escherichia... 50.4 2e-10
P0AEB5 Low conductance mechanosensitive channel YnaI [Escherichia... 50.4 2e-10
Q3E7D0 Nucleobase-ascorbate transporter 12 [Arabidopsis thaliana] 36.2 8e-06
>P0AAT5 Miniconductance mechanosensitive channel YbdG [Shigella
flexneri]
Length=415
Score = 310 bits (795), Expect = 3e-106
Identities = 165/392 (42%), Positives = 248/392 (63%), Gaps = 3/392 (1%)
Query 28 MLTSLSILILLAILANFIAKQIVVRGIRKAISKIKFLNQSIFAQHSVIKRIANIVPAIVI 87
M+ I+ L A++ + I +V+R K L I Q+ + R+A + I++
Sbjct 22 MVMIFGIIFLTAVVVHIILHWVVLRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIV 81
Query 88 -MNGITTVPHLSEKFIIFTQMGAQAFIFLTIALAISEFLNIFNLVYQRNPNSKNKPIKGY 146
+ + + +E I T AQ +I + L++ L++ + Q+ P + P+KG
Sbjct 82 NIQAVFWLQKGTEAADILTTC-AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGI 140
Query 147 LQLVKLMIFIVCALMVLGTFLKKDVFTLLAGFGAMATVLMLVFQNTILSLVASVQISSYD 206
Q +KL+ I+ ++++ + + L++G GAMA VLMLVF++ IL LVA +Q+S+ D
Sbjct 141 FQGIKLIGAILVGILMISLLIGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSAND 200
Query 207 MVRIGDWIEMPSLNADGDVIDMSLHTVTVQNFDKTFTTIPTNKLITDTFKNWRGMSQAGC 266
M+++GDW+EMP ADG VID+ L TV V+N+D T TTIPT L++D+FKNW GMS +G
Sbjct 201 MLKLGDWLEMPKYGADGAVIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGG 260
Query 267 RRIKRSLFIDQSSVHFMSETEQEKLKNFILLNQYLDLKKSEITSFNHQL-SNQSLYNQRR 325
RRIKRS+ ID +S+ F+ E E ++L LL YL + EI +N Q S +S+ N RR
Sbjct 261 RRIKRSISIDVTSIRFLDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRR 320
Query 326 LTNLGTFRAYIEFYLRQHSGIAQNQSLMVRQLQPTSEGLPLEIYAFTNTTAWVAYEAIQS 385
+TN+GTFRAY+ YLR H I ++ +LMVRQL P GLPLEIYAFTNT W+ YE+IQ+
Sbjct 321 MTNIGTFRAYLNEYLRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQA 380
Query 386 DIFDHLLAIIPEFGLKIYQAPSGADFKLLSSS 417
DIFDH+ AI+ EFGL+++Q+P+G D + L+ +
Sbjct 381 DIFDHIFAIVEEFGLRLHQSPTGNDIRSLAGA 412
Score = 23.5 bits (49), Expect = 0.067
Identities = 24/91 (26%), Positives = 37/91 (41%), Gaps = 17/91 (19%)
Query 2 KGVEVLDFLKFIHDVERWIDQYPWLEMLTSLSILILLAILANF-----IAKQIVVRGIRK 56
KG E D L + W+ M LS+ LL ++ N A Q+ ++GI +
Sbjct 91 KGTEAADILTTCAQL--------WIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQ 142
Query 57 AISKIKFLNQSIFAQHSVIKRIANIVPAIVI 87
I I + I +I + PAI+I
Sbjct 143 GIKLIGAILVGIL----MISLLIGQSPAILI 169
>P0AAT4 Miniconductance mechanosensitive channel YbdG [Escherichia
coli (strain K12)]
Length=415
Score = 310 bits (795), Expect = 3e-106
Identities = 165/392 (42%), Positives = 248/392 (63%), Gaps = 3/392 (1%)
Query 28 MLTSLSILILLAILANFIAKQIVVRGIRKAISKIKFLNQSIFAQHSVIKRIANIVPAIVI 87
M+ I+ L A++ + I +V+R K L I Q+ + R+A + I++
Sbjct 22 MVMIFGIIFLTAVVVHIILHWVVLRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIV 81
Query 88 -MNGITTVPHLSEKFIIFTQMGAQAFIFLTIALAISEFLNIFNLVYQRNPNSKNKPIKGY 146
+ + + +E I T AQ +I + L++ L++ + Q+ P + P+KG
Sbjct 82 NIQAVFWLQKGTEAADILTTC-AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGI 140
Query 147 LQLVKLMIFIVCALMVLGTFLKKDVFTLLAGFGAMATVLMLVFQNTILSLVASVQISSYD 206
Q +KL+ I+ ++++ + + L++G GAMA VLMLVF++ IL LVA +Q+S+ D
Sbjct 141 FQGIKLIGAILVGILMISLLIGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSAND 200
Query 207 MVRIGDWIEMPSLNADGDVIDMSLHTVTVQNFDKTFTTIPTNKLITDTFKNWRGMSQAGC 266
M+++GDW+EMP ADG VID+ L TV V+N+D T TTIPT L++D+FKNW GMS +G
Sbjct 201 MLKLGDWLEMPKYGADGAVIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGG 260
Query 267 RRIKRSLFIDQSSVHFMSETEQEKLKNFILLNQYLDLKKSEITSFNHQL-SNQSLYNQRR 325
RRIKRS+ ID +S+ F+ E E ++L LL YL + EI +N Q S +S+ N RR
Sbjct 261 RRIKRSISIDVTSIRFLDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRR 320
Query 326 LTNLGTFRAYIEFYLRQHSGIAQNQSLMVRQLQPTSEGLPLEIYAFTNTTAWVAYEAIQS 385
+TN+GTFRAY+ YLR H I ++ +LMVRQL P GLPLEIYAFTNT W+ YE+IQ+
Sbjct 321 MTNIGTFRAYLNEYLRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQA 380
Query 386 DIFDHLLAIIPEFGLKIYQAPSGADFKLLSSS 417
DIFDH+ AI+ EFGL+++Q+P+G D + L+ +
Sbjct 381 DIFDHIFAIVEEFGLRLHQSPTGNDIRSLAGA 412
Score = 23.5 bits (49), Expect = 0.067
Identities = 24/91 (26%), Positives = 37/91 (41%), Gaps = 17/91 (19%)
Query 2 KGVEVLDFLKFIHDVERWIDQYPWLEMLTSLSILILLAILANF-----IAKQIVVRGIRK 56
KG E D L + W+ M LS+ LL ++ N A Q+ ++GI +
Sbjct 91 KGTEAADILTTCAQL--------WIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQ 142
Query 57 AISKIKFLNQSIFAQHSVIKRIANIVPAIVI 87
I I + I +I + PAI+I
Sbjct 143 GIKLIGAILVGIL----MISLLIGQSPAILI 169
>Q58543 Large-conductance mechanosensitive channel MscMJLR [Methanocaldococcus
jannaschii (strain ATCC 43067 / DSM 2661 /
JAL-1 / JCM 10045 / NBRC 100440)]
Length=361
Score = 57.0 bits (136), Expect = 2e-12
Identities = 33/110 (30%), Positives = 60/110 (55%), Gaps = 3/110 (3%)
Query 148 QLVKLMIFIVCALMVLGTFLKKDVFTLLAGFGAMATVLMLVFQNTILSLVASVQISSYDM 207
+LV+L++++V L++L L D+ TLLAG G + L QN + +L+A + I +
Sbjct 143 KLVRLVVWVVGLLLILSN-LGYDIKTLLAGLGIGGLAVALASQNLVSNLIAGLIILTDKP 201
Query 208 VRIGDWIEMPSLNADGDVIDMSLHTVTVQNFDKTFTTIPTNKLITDTFKN 257
+IG+WI G V D+ + + ++ D + +P +KLI + +N
Sbjct 202 FKIGNWITFS--GGSGIVEDIGIRSTKIRATDNSIIVVPNSKLIDEIIQN 249
Score = 16.5 bits (31), Expect = 9.7
Identities = 6/22 (27%), Positives = 12/22 (55%), Gaps = 0/22 (0%)
Query 127 IFNLVYQRNPNSKNKPIKGYLQ 148
I + +PN +++PI Y +
Sbjct 279 IIKNILLEHPNVEDEPITVYFK 300
>P0AEB6 Low conductance mechanosensitive channel YnaI [Escherichia
coli O157:H7]
Length=343
Score = 50.4 bits (119), Expect = 2e-10
Identities = 60/278 (22%), Positives = 103/278 (37%), Gaps = 61/278 (22%)
Query 148 QLVKLMIFIVCALMVLGTFLKKDVFTLLAGFGAMATVLMLVFQNTILSLVASVQISSYDM 207
+++K+ I +V L+ G + LL FG + + + + ILS S + +D
Sbjct 120 RIIKITIIVVLVLLY-GEHFGMSLSGLLT-FGGIGGLAVGMAGKDILSNFFSGIMLYFDR 177
Query 208 -VRIGDWIEMPSLNADGDVIDMSLHTVTVQNFDKTFTTIPTNKLITDTFKNWRGMSQAGC 266
IGDWI P N +G V ++ + FD +P + + + +N M+
Sbjct 178 PFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTN--- 234
Query 267 RRIKRSLFIDQSSVHFMSETEQEKLKNFILLNQYLDLKKSEITSFNHQLSNQSLYNQRRL 326
RRI ++ + +Y D K
Sbjct 235 RRITTTIGL-----------------------RYEDAAK--------------------- 250
Query 327 TNLGTFRAYIEFYLRQHSGIAQNQSLMVRQLQPTSEGLPLEIYAFTNTTAWVAYEAIQSD 386
+G + L+ H I Q Q+L+V Q L + +Y FT TT W + A Q D
Sbjct 251 --VGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQD 308
Query 387 IFDHLLAIIPEFGLKIYQAPSGADFKLLSSSIVSEENT 424
++ ++ I+ GADF S ++ + T
Sbjct 309 VYLKIIDIVQS---------HGADFAFPSQTLYMDNIT 337
Score = 16.9 bits (32), Expect = 7.3
Identities = 5/10 (50%), Positives = 9/10 (90%), Gaps = 0/10 (0%)
Query 291 LKNFILLNQY 300
+K F+L+NQ+
Sbjct 92 IKLFLLINQF 101
>P0AEB5 Low conductance mechanosensitive channel YnaI [Escherichia
coli (strain K12)]
Length=343
Score = 50.4 bits (119), Expect = 2e-10
Identities = 60/278 (22%), Positives = 103/278 (37%), Gaps = 61/278 (22%)
Query 148 QLVKLMIFIVCALMVLGTFLKKDVFTLLAGFGAMATVLMLVFQNTILSLVASVQISSYDM 207
+++K+ I +V L+ G + LL FG + + + + ILS S + +D
Sbjct 120 RIIKITIIVVLVLLY-GEHFGMSLSGLLT-FGGIGGLAVGMAGKDILSNFFSGIMLYFDR 177
Query 208 -VRIGDWIEMPSLNADGDVIDMSLHTVTVQNFDKTFTTIPTNKLITDTFKNWRGMSQAGC 266
IGDWI P N +G V ++ + FD +P + + + +N M+
Sbjct 178 PFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTN--- 234
Query 267 RRIKRSLFIDQSSVHFMSETEQEKLKNFILLNQYLDLKKSEITSFNHQLSNQSLYNQRRL 326
RRI ++ + +Y D K
Sbjct 235 RRITTTIGL-----------------------RYEDAAK--------------------- 250
Query 327 TNLGTFRAYIEFYLRQHSGIAQNQSLMVRQLQPTSEGLPLEIYAFTNTTAWVAYEAIQSD 386
+G + L+ H I Q Q+L+V Q L + +Y FT TT W + A Q D
Sbjct 251 --VGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQD 308
Query 387 IFDHLLAIIPEFGLKIYQAPSGADFKLLSSSIVSEENT 424
++ ++ I+ GADF S ++ + T
Sbjct 309 VYLKIIDIVQS---------HGADFAFPSQTLYMDNIT 337
Score = 16.9 bits (32), Expect = 7.3
Identities = 5/10 (50%), Positives = 9/10 (90%), Gaps = 0/10 (0%)
Query 291 LKNFILLNQY 300
+K F+L+NQ+
Sbjct 92 IKLFLLINQF 101
>Q3E7D0 Nucleobase-ascorbate transporter 12 [Arabidopsis thaliana]
Length=709
Score = 36.2 bits (82), Expect = 8e-06
Identities = 40/144 (28%), Positives = 63/144 (44%), Gaps = 23/144 (16%)
Query 76 KRIANIVPAIVIMNGITTVPHLSEKFIIFTQMGAQAFIFLTIALAISEFLNIFNLVYQRN 135
+ +AN+V ++ ++GITT+ H S + G +F+FL ALAI L N
Sbjct 213 EEVANVVSTVLFVSGITTLLHTSFGSRLPLIQG-PSFVFLAPALAIINSPEFQGL----N 267
Query 136 PNSKNKPIKGYLQLVKLMIFIVCALMVLGTFLKKDVFTLLAGF-GAMATVLMLVFQNTIL 194
N+ K I LQ G + F + G+ G M+ +L LV +
Sbjct 268 GNNNFKHIMRELQ---------------GAIIIGSAFQAVLGYSGLMSLILRLVNPVVVA 312
Query 195 SLVASVQIS--SYDMVRIGDWIEM 216
VA+V +S SY +G +E+
Sbjct 313 PTVAAVGLSFYSYGFPLVGKCLEI 336
Score = 18.1 bits (35), Expect = 3.4
Identities = 7/23 (30%), Positives = 14/23 (61%), Gaps = 0/23 (0%)
Query 209 RIGDWIEMPSLNADGDVIDMSLH 231
RI + +E+ + D D++ +LH
Sbjct 146 RIEETVEVLPQSMDDDLVARNLH 168
Lambda K H a alpha
0.327 0.139 0.398 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 889326
Database: 66948fe2db9f26d7a0181436751a139e.SwissProt.fasta
Posted date: May 31, 2024 4:17 AM
Number of letters in database: 2,586
Number of sequences in database: 6
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
