Comparative Genomics

ACIAD2016 / SwissProt Alignments

BLASTP 2.12.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.


Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.



Database: c1a235397cd14224aa9c42fb0032c7ca.SwissProt.fasta
           10 sequences; 3,399 total letters



Query= ACIAD2016

Length=317
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

Q6IE26 Epoxide hydrolase 4 [Mus musculus]                             52.4    3e-11
Q8IUS5 Epoxide hydrolase 4 [Homo sapiens]                             49.3    3e-10
O06734 AB hydrolase superfamily protein YisY [Bacillus subtilis (...  45.8    3e-09
O05235 Uncharacterized hydrolase YugF [Bacillus subtilis (strain ...  44.7    8e-09
Q9H6B9 Epoxide hydrolase 3 [Homo sapiens]                             44.7    1e-08
P75311 Putative esterase/lipase 2 [Mycoplasma pneumoniae (strain ...  38.9    6e-07
Q9FJ24 Probable lysophospholipase BODYGUARD 2 [Arabidopsis thaliana]  38.1    2e-06
P73883 Uncharacterized protein sll0249 [Synechocystis sp. (strain...  35.4    8e-06
Q54M29 Serine hydrolase-like protein DDB_G0286239 [Dictyostelium ...  35.4    1e-05
O14249 Probable cardiolipin-specific deacylase 1, mitochondrial [...  35.4    1e-05


>Q6IE26 Epoxide hydrolase 4 [Mus musculus]
Length=359

 Score = 52.4 bits (124),  Expect = 3e-11
 Identities = 35/107 (33%), Positives = 55/107 (51%), Gaps = 8/107 (7%)

Query  45   VLYIHGFLDYFFQTQMAEQFNQHGYDFYALDLRKYGRSKLP-HQIFYNVLDLKEYDAEIS  103
            +L +HGF ++++  +   +  +  Y   ALDLR YG S  P HQ  Y +      D  I+
Sbjct  94   MLLLHGFPEFWYSWRHQLREFKSEYRVVALDLRGYGESDAPAHQESYKL------DCLIA  147

Query  104  AALDIIQQEQHQEILLAGHSTGGLIATLYAAHYPEQHSIRAVWVNSP  150
               DI+    + + +L GH  GG+IA L A  YPE   ++ + +N P
Sbjct  148  DIKDILDSLGYSKCVLIGHDWGGMIAWLIAVCYPEM-IMKLIVINFP  193


>Q8IUS5 Epoxide hydrolase 4 [Homo sapiens]
Length=362

 Score = 49.3 bits (116),  Expect = 3e-10
 Identities = 33/107 (31%), Positives = 55/107 (51%), Gaps = 8/107 (7%)

Query  45   VLYIHGFLDYFFQTQMAEQFNQHGYDFYALDLRKYGRSKLP-HQIFYNVLDLKEYDAEIS  103
            +L +HGF ++++  +   +  +  Y   ALDLR YG +  P H+  Y +      D  I+
Sbjct  96   MLLLHGFPEFWYSWRYQLREFKSEYRVVALDLRGYGETDAPIHRQNYKL------DCLIT  149

Query  104  AALDIIQQEQHQEILLAGHSTGGLIATLYAAHYPEQHSIRAVWVNSP  150
               DI+    + + +L GH  GG+IA L A  YPE   ++ + +N P
Sbjct  150  DIKDILDSLGYSKCVLIGHDWGGMIAWLIAICYPEM-VMKLIVINFP  195


>O06734 AB hydrolase superfamily protein YisY [Bacillus subtilis 
(strain 168)]
Length=268

 Score = 45.8 bits (107),  Expect = 3e-09
 Identities = 50/222 (23%), Positives = 96/222 (43%), Gaps = 28/222 (13%)

Query  38   NINTSRAVLYIHGF-LDY-FFQTQMAEQFNQHGYDFYALDLRKYGRSKLPHQIFYNVLDL  95
            +I   R ++++HG+ L++  F+ QM E   + G+ F  +DLR YG+S  P + +      
Sbjct  17   DIGHGRPIIFLHGWPLNHKMFEYQMNE-LPKRGFRFIGVDLRGYGQSDRPWEGY------  69

Query  96   KEYDAEISAALDIIQQEQHQEILLAGHSTGGLIATLYAAHYPEQHSIRAVWVNSPFYEFN  155
             +YD        +I   Q +  +LAG S GG IA  Y A +      + + +++    F 
Sbjct  70   -DYDTMADDVKAVIYTLQLENAILAGFSMGGAIAIRYMARHEGADVDKLILLSAAAPAFT  128

Query  156  LNFIEKKLGIPV---LSQFGRVLPKFKF--PSKL----NRWYVTSLHHSLKGEWAFNLDW  206
                 K+ G P          ++  FK   P  L     +++   +   L+ +W  NL  
Sbjct  129  -----KRPGYPYGMRKQDIDDMIELFKADRPKTLADLGKQFFEKKVSPELR-QWFLNLML  182

Query  207  KPKTASTVQLSFIHAIHEAQKEIHKGIKLNIPLLVMHSHQTK  248
            +  +  T+         + +KE+     + +P L++H  + +
Sbjct  183  EASSYGTIHSGIALRDEDLRKEL---AAIKVPTLILHGRKDR  221


>O05235 Uncharacterized hydrolase YugF [Bacillus subtilis (strain 
168)]
Length=273

 Score = 44.7 bits (104),  Expect = 8e-09
 Identities = 28/99 (28%), Positives = 46/99 (46%), Gaps = 6/99 (6%)

Query  43   RAVLYIHGFLDYFFQTQMAEQFNQHGYDFYALDLRKYGRSKLPHQIFYNVLDLKEYDAEI  102
            + ++ +HGFL   F  +      +  YD  ALDL  +G+S+      Y   +L +     
Sbjct  28   QTLVCVHGFLSSAFSFRKVIPLLRDKYDIIALDLPPFGQSEKSRTFIYTYQNLAK-----  82

Query  103  SAALDIIQQEQHQEILLAGHSTGGLIATLYAAHYPEQHS  141
               + I++  Q ++ +L GHS GG I+   A   PE  S
Sbjct  83   -LVIGILEHLQVKQAVLVGHSMGGQISLSAALQKPELFS  120


 Score = 21.6 bits (44),  Expect = 0.23
 Identities = 11/47 (23%), Positives = 22/47 (47%), Gaps = 0/47 (0%)

Query  25   EGKVVATLVRKKSNINTSRAVLYIHGFLDYFFQTQMAEQFNQHGYDF  71
            E ++V   + K+ + +   +VLY  G   +    +  E  ++H  DF
Sbjct  225  EDRIVPMEIGKRLHADLPNSVLYSLGQTGHLVPEERPELISEHIADF  271


>Q9H6B9 Epoxide hydrolase 3 [Homo sapiens]
Length=360

 Score = 44.7 bits (104),  Expect = 1e-08
 Identities = 30/120 (25%), Positives = 57/120 (48%), Gaps = 8/120 (7%)

Query  45   VLYIHGFLDYFFQTQMAEQFNQHGYDFYALDLRKYGRSKLPHQIFYNVLDLKEYDAEISA  104
            +L++HGF + +F  +   +  Q  +   A+DLR YG S  P  +    +DL   D +   
Sbjct  100  MLFLHGFPENWFSWRYQLREFQSRFHVVAVDLRGYGPSDAPRDVDCYTIDLLLVDIK---  156

Query  105  ALDIIQQEQHQEILLAGHSTGGLIATLYAAHYPEQHSIRAVWVNSP---FYEFNLNFIEK  161
              D+I    + + +L  H  G L+A  ++ +YP       V   +P   + +++L+ I +
Sbjct  157  --DVILGLGYSKCILVAHDWGALLAWHFSIYYPSLVERMVVVSGAPMSVYQDYSLHHISQ  214


>P75311 Putative esterase/lipase 2 [Mycoplasma pneumoniae (strain 
ATCC 29342 / M129 / Subtype 1)]
Length=268

 Score = 38.9 bits (89),  Expect = 6e-07
 Identities = 30/119 (25%), Positives = 61/119 (51%), Gaps = 9/119 (8%)

Query  46   LYIHGF-LDYFFQTQMAEQFNQHGYDFYALDLRKYGRSKLPHQIFYNVLDLKEYDAEISA  104
            +++HGF  +Y     + + F +  + F+A +   +G ++       + L LK Y   +  
Sbjct  25   IFLHGFGSEYSSFKHVFKLFEKKRWSFFAFNFPGHGNNQSNS---VDELKLKHY---VEL  78

Query  105  ALDIIQQEQHQEILLAGHSTGGLIATLYAAHYPEQHSIRAVWVNSPFYEFNLNFIEKKL  163
              D I Q++ ++++L GHS GG IA L  A   E+  I+A+ + +P  + +    +K++
Sbjct  79   VCDFIIQKRLKKVVLVGHSMGGAIAVLVNAVLRER--IKALVLVAPMNQTSFVVSKKRI  135


>Q9FJ24 Probable lysophospholipase BODYGUARD 2 [Arabidopsis thaliana]
Length=471

 Score = 38.1 bits (87),  Expect = 2e-06
 Identities = 33/122 (27%), Positives = 59/122 (48%), Gaps = 12/122 (10%)

Query  36   KSNINTSRAVLYIHGFLDY--FFQTQMAEQFN---QHGYDFYALDLRKYGRSKLPHQIFY  90
            K N      V++IHGF+    F+   +   F+   +  Y F A+DL  YGRS  P+   Y
Sbjct  184  KDNKKARDNVVFIHGFVSSSAFWTETLFPNFSDSAKSNYRFIAVDLLGYGRSPKPNDSLY  243

Query  91   NVLDLKEYDAEISAALDIIQQEQHQEILLAGHSTGGLIATLYAAHYPEQHSIRAVWVNSP  150
                L+E+   I  +  +I + + +   +  HS G ++A   A  +P   +I+++ + +P
Sbjct  244  T---LREHLEMIEKS--VISKFKLKTFHIVAHSLGCILALALAVKHP--GAIKSLTLLAP  296

Query  151  FY  152
             Y
Sbjct  297  PY  298


 Score = 19.6 bits (39),  Expect = 1.0
 Identities = 9/32 (28%), Positives = 17/32 (53%), Gaps = 0/32 (0%)

Query  223  HEAQKEIHKGIKLNIPLLVMHSHQTKNPKKWN  254
            HE++K+I KG K  + L      ++    +W+
Sbjct  126  HESKKKISKGKKRLMTLNSTVIEKSSTAPRWS  157


>P73883 Uncharacterized protein sll0249 [Synechocystis sp. (strain 
PCC 6803 / Kazusa)]
Length=251

 Score = 35.4 bits (80),  Expect = 8e-06
 Identities = 18/41 (44%), Positives = 25/41 (61%), Gaps = 3/41 (7%)

Query  102  ISAALDIIQQ---EQHQEILLAGHSTGGLIATLYAAHYPEQ  139
            ++  LD+I +     HQ + L GHST GL+A LYA   PE+
Sbjct  53   LTVGLDLIGEYLENFHQPVHLIGHSTSGLLALLYARQCPEK  93


>Q54M29 Serine hydrolase-like protein DDB_G0286239 [Dictyostelium 
discoideum]
Length=359

 Score = 35.4 bits (80),  Expect = 1e-05
 Identities = 31/102 (30%), Positives = 50/102 (49%), Gaps = 14/102 (14%)

Query  41   TSRAVLYIHGFLDY-----FFQTQMAEQFNQHGYDFYALDLRKYGRSKLPHQIFYNVLDL  95
            +S+ +L +HG+LD      F    +AE+    G    A+D   +G S  PH+  +  L  
Sbjct  33   SSQKMLALHGWLDNANTFDFIAPILAEK----GIRIIAIDFIGHGLS--PHKPSWCNLYY  86

Query  96   KEYDAEISAALDIIQQEQHQEILLAGHSTGGLIATLYAAHYP  137
             +Y   I+  LD+ +  Q +   + GHS G  IA++ AA  P
Sbjct  87   TDY---ITQVLDVAEALQWKTFSIMGHSMGAGIASIVAASMP  125


>O14249 Probable cardiolipin-specific deacylase 1, mitochondrial 
[Schizosaccharomyces pombe (strain 972 / ATCC 24843)]
Length=428

 Score = 35.4 bits (80),  Expect = 1e-05
 Identities = 31/130 (24%), Positives = 61/130 (47%), Gaps = 17/130 (13%)

Query  23   DYEGKVVATLVRKKSNINTS-RAVLYIHGF---LDYFFQTQ------MAEQFNQHGYDFY  72
            D +G V    V  K+    + R+++Y+HG+   L ++F+        + + FN +  D+ 
Sbjct  71   DNDGLVHEVCVSDKATGKANKRSIVYMHGYGAGLGFYFRNMDGLTKGVTKDFNSYFVDWL  130

Query  73   ALDLRKYGRSKLPHQIFYNVLDLKEYDAE--ISAALDIIQQEQH-QEILLAGHSTGGLIA  129
             +       S+ P  I       K  + E   + +L+  +     ++++L GHS GG ++
Sbjct  131  GMG----NSSRPPFDIKGQTASEKVEETERFFTESLETWRIGHGIEKMILVGHSMGGYLS  186

Query  130  TLYAAHYPEQ  139
             +YA  YPE+
Sbjct  187  AVYAMQYPER  196



Lambda      K        H        a         alpha
   0.322    0.137    0.416    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 807807


  Database: c1a235397cd14224aa9c42fb0032c7ca.SwissProt.fasta
    Posted date:  May 29, 2024  11:49 PM
  Number of letters in database: 3,399
  Number of sequences in database:  10



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40