BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: a31930cc2edf771a42891792289375fc.SwissProt.fasta
1 sequences; 4,538 total letters
Query= ACIAD2196
Length=265
Score E
Sequences producing significant alignments: (Bits) Value
Q05470 Polyketide synthase PksL [Bacillus subtilis (strain 168)] 35.0 2e-05
>Q05470 Polyketide synthase PksL [Bacillus subtilis (strain 168)]
Length=4538
Score = 35.0 bits (79), Expect = 2e-05
Identities = 29/92 (32%), Positives = 41/92 (45%), Gaps = 9/92 (10%)
Query 29 MNAIAKFTSTNPNGLALLKQNQAWLEACLENENVCHYFAIQIKGKESYPF--GAEDRPFF 86
+ +I + S P GL +K N C E A+ +K ++ PF G E+ P+F
Sbjct 4386 IQSIYRSESKAPLGLGSVKPNIGH-PLCAEGIASLIKVALMLKHRQLVPFLSGNENMPYF 4444
Query 87 DLEKAQIYLEHLQA----TNP--NINYFISSG 112
D+EK +Y QA T P IN F G
Sbjct 4445 DIEKTDLYFSRSQAEWKETTPAAAINCFADGG 4476
Score = 21.9 bits (45), Expect = 0.23
Identities = 10/33 (30%), Positives = 18/33 (55%), Gaps = 2/33 (6%)
Query 67 AIQIKGKESYP--FGAEDRPFFDLEKAQIYLEH 97
A+Q+ ++ P E P+ D EK+ Y++H
Sbjct 798 ALQLHHQKLVPSLHSEELNPYVDFEKSPFYVQH 830
Score = 19.2 bits (38), Expect = 1.6
Identities = 19/98 (19%), Positives = 35/98 (36%), Gaps = 6/98 (6%)
Query 161 YNEIKIWQEEHNTKEICHCYTAQSFDDSNGDISISSQFTTNLMTALSAKIYFEKTMSNRN 220
+N +KI K + Q +D + + S T+ + + +TM
Sbjct 3729 WNSVKISSLAEKIKAV------QELEDMGAQVQVLSLDLTDRVAVEQSLKTIHETMGAIG 3782
Query 221 FRVICGLMTTEQVMGMDGKVNEELQDFIDQHKARLQSL 258
+ C M +Q K EE+ ++ LQ+L
Sbjct 3783 GVIHCAGMVNKQNPAFIRKSLEEIGQVLEPKVEGLQTL 3820
Score = 18.5 bits (36), Expect = 2.8
Identities = 5/15 (33%), Positives = 12/15 (80%), Gaps = 0/15 (0%)
Query 162 NEIKIWQEEHNTKEI 176
+EIK+++ +H+ K +
Sbjct 3396 SEIKLFETDHDAKAL 3410
Score = 18.5 bits (36), Expect = 2.8
Identities = 9/37 (24%), Positives = 16/37 (43%), Gaps = 0/37 (0%)
Query 65 YFAIQIKGKESYPFGAEDRPFFDLEKAQIYLEHLQAT 101
Y+A + + Y G R + + A+ L H Q +
Sbjct 3646 YYAKSTESEVCYRNGQRYRAYLTEQPAEAALSHKQVS 3682
Score = 17.3 bits (33), Expect = 6.3
Identities = 6/14 (43%), Positives = 8/14 (57%), Gaps = 0/14 (0%)
Query 97 HLQATNPNINYFIS 110
H NPNI++ S
Sbjct 2305 HSNVLNPNIDFLNS 2318
Score = 17.3 bits (33), Expect = 6.3
Identities = 6/17 (35%), Positives = 9/17 (53%), Gaps = 0/17 (0%)
Query 123 DENLPMWHRVWLNKHQY 139
DE++ WL K +Y
Sbjct 2478 DEDMSKTIEAWLQKGKY 2494
Score = 16.9 bits (32), Expect = 8.3
Identities = 7/19 (37%), Positives = 12/19 (63%), Gaps = 0/19 (0%)
Query 191 DISISSQFTTNLMTALSAK 209
D+S+SS + + LSA+
Sbjct 880 DVSLSSDSDISAVIPLSAR 898
Score = 16.9 bits (32), Expect = 8.3
Identities = 9/27 (33%), Positives = 14/27 (52%), Gaps = 1/27 (4%)
Query 70 IKGKESYPFGAEDRPFFDLEKAQIYLE 96
+ G ++Y A FFDL+ I L+
Sbjct 4209 VAGGQNY-LAANISQFFDLKGPSIVLD 4234
Lambda K H a alpha
0.321 0.133 0.396 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 988460
Database: a31930cc2edf771a42891792289375fc.SwissProt.fasta
Posted date: May 8, 2024 2:26 PM
Number of letters in database: 4,538
Number of sequences in database: 1
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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