BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: b83636c35f139c6d7221db81341b55ab.SwissProt.fasta
11 sequences; 2,183 total letters
Query= ACIAD2355
Length=209
Score E
Sequences producing significant alignments: (Bits) Value
A0A0H3H7Y9 Type II secretion system protein J [Klebsiella michiga... 70.1 1e-18
Q00517 Type II secretion system protein J [Pseudomonas aeruginosa... 69.7 3e-18
P15749 Type II secretion system protein J [Klebsiella pneumoniae] 66.2 3e-17
P31739 Type II secretion system protein J [Aeromonas hydrophila] 58.2 3e-14
P24689 Type II secretion system protein J [Dickeya chrysanthemi] 57.0 7e-14
P31589 Type II secretion system protein J [Pectobacterium carotov... 53.9 1e-12
A0A4C3GMC1 Type II secretion system protein J [Escherichia coli] 52.8 2e-12
P45761 Type II secretion system protein J [Escherichia coli (stra... 47.8 1e-10
P45776 Type II secretion system protein J [Vibrio cholerae seroty... 40.8 3e-08
P36647 Prepilin peptidase-dependent protein D [Escherichia coli (... 34.7 3e-06
P35646 Fimbrial protein EcpB [Eikenella corrodens] 34.3 4e-06
>A0A0H3H7Y9 Type II secretion system protein J [Klebsiella michiganensis
(strain ATCC 8724 / DSM 4798 / JCM 20051 / NBRC 3318
/ NRRL B-199 / KCTC 1686 / BUCSAV 143 / CCM 1901)]
Length=199
Score = 70.1 bits (170), Expect = 1e-18
Identities = 58/191 (30%), Positives = 98/191 (51%), Gaps = 20/191 (10%)
Query 3 RHLGFTLIELLVSIAIFAILSALGWKIFDYLIKIKDRNQVYEQQLFELQDAYQLLLRDSL 62
R GFTL+E+L+++AIFA LS ++I +++ + Q Q+L ELQ A+ L D
Sbjct 5 RERGFTLLEMLLALAIFAALSLSAFQILQGVMRNDEMAQRQVQRLTELQRAFVYLEGDFG 64
Query 63 QIIPNTANINGQL----RPALSLSENILQFSRGGVSDPLKEGLAP---YERIEYRYVASE 115
QIIP + +L R ++ + F R G +P+ G+ P +R+ YR
Sbjct 65 QIIPRPPRGDERLFYAARYQRQSADWSISFMRNGWQNPM--GILPRSELQRVGYR--LRH 120
Query 116 KVVYRLKYKNLNVVSSEQPVSSVVLKNVDQFQIAVLNPQLSTQWPDPQIDLTQSQNLRIL 175
+ + RL Y + + + E+P+ V+LK+V F++ + W D D T+ L
Sbjct 121 QQLERLSYVHTDPQAGEEPIVKVLLKDVSAFRLRFF---ANGMWRDSWNDTTR------L 171
Query 176 PRGLKVNLNIA 186
P G++V+L +A
Sbjct 172 PEGIEVSLVVA 182
>Q00517 Type II secretion system protein J [Pseudomonas aeruginosa
(strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847
/ LMG 12228 / 1C / PRS 101 / PAO1)]
Length=237
Score = 69.7 bits (169), Expect = 3e-18
Identities = 54/187 (29%), Positives = 95/187 (51%), Gaps = 6/187 (3%)
Query 1 MKRHLGFTLIELLVSIAIFAILSALGWKIFDYLIKIKDRNQVYEQQLFELQDAYQLLLRD 60
M+ GFTL+ELL++IAIFA+L+ +++FD +++ +V EQ++ EL A L RD
Sbjct 1 MRLQRGFTLLELLIAIAIFALLALATYRMFDSVMQTDQATRVQEQRMRELVRAMGALERD 60
Query 61 SLQII--PNTANINGQLRPALSLSEN--ILQFSRGGVSDPLKEGLAPYERIEYRYVASEK 116
Q + P + LS EN I++F+RGG +PL + + +R+ R+ S +
Sbjct 61 LTQAVERPVRDELGDNRGAFLSEGENDQIVEFTRGGWRNPLGQARSRLQRV--RWSLSGE 118
Query 117 VVYRLKYKNLNVVSSEQPVSSVVLKNVDQFQIAVLNPQLSTQWPDPQIDLTQSQNLRILP 176
+ R + L+ +P VL V L+ + + Q P + ++ + L LP
Sbjct 119 TLERRYWLVLDRAQDSKPRVQQVLDGVTALSWRFLDKEHNWQGHWPTDEGSEEERLESLP 178
Query 177 RGLKVNL 183
+++ L
Sbjct 179 LAVEMTL 185
>P15749 Type II secretion system protein J [Klebsiella pneumoniae]
Length=198
Score = 66.2 bits (160), Expect = 3e-17
Identities = 54/192 (28%), Positives = 91/192 (47%), Gaps = 20/192 (10%)
Query 1 MKRHLGFTLIELLVSIAIFAILSALGWKIFDYLIKIKDRNQVYEQQLFELQDAYQLLLRD 60
++R GFTL+E+L+++AI A LS + +++ + ++ LQ + + D
Sbjct 2 IRRSSGFTLVEMLLALAILAALSVAAVTVLQNVMRADTLTRDKSGRMQALQQTFSQMAAD 61
Query 61 SLQIIPNTANINGQL----RPALSLSENILQFSRGGVSDPLKEGLAP---YERIEYRYVA 113
QIIP + + L R L + + FSR G +PL GL P + + YR
Sbjct 62 FSQIIPRRSRDSASLFFAGRFQLGSDDGAIAFSRNGWPNPL--GLLPRSEIQNVSYRLRG 119
Query 114 SEKVVYRLKYKNLNVVSSEQPVSSVVLKNVDQFQIAVLNPQLSTQWPDPQIDLTQSQNLR 173
S+ + RL Y + + QP ++VL++V F L S +W D + Q +
Sbjct 120 SQ--LERLNYDQQDPLPGSQPTVTIVLRDVRAFG---LRFYASGRWQD------EWQQAQ 168
Query 174 ILPRGLKVNLNI 185
LP+GL+V L +
Sbjct 169 TLPQGLEVTLTL 180
>P31739 Type II secretion system protein J [Aeromonas hydrophila]
Length=207
Score = 58.2 bits (139), Expect = 3e-14
Identities = 54/194 (28%), Positives = 93/194 (48%), Gaps = 24/194 (12%)
Query 3 RHLGFTLIELLVSIAIFAILSALGWKIFDYLIKIKDRNQVYEQQLFELQDAYQLLLRDSL 62
R GFTL+E+LV+IAIFA LS +++ ++ + + E +L ELQ A+ L+ RD
Sbjct 4 RQGGFTLLEMLVAIAIFASLSMGAYQVLQGVLTSDEVAKKKEARLSELQLAFTLMERDIT 63
Query 63 QIIPNTANINGQLRPALSLSENI--------LQFSRGGVSDPLKEGLAP---YERIEYRY 111
Q++P + I+G+ L + + F RGG +P +G+ P +R+ +R
Sbjct 64 QMVPRSGRIDGENNKVLLAASRFGQQSDDWGMAFIRGGWLNP--DGMLPRSGLQRVGWR- 120
Query 112 VASEKVVYRLKYKNLNVVSSEQPVSSVVLKNVDQFQIAVLNPQLSTQWPDPQIDLTQSQN 171
+ + RL Y + V +P +L V ++ + W + + TQ
Sbjct 121 -LKDHKLERLSYLYPDPVIGTEPRIQPMLDRVTALRLYFYD---KGAWKE---EWTQR-- 171
Query 172 LRILPRGLKVNLNI 185
+LP GL V L++
Sbjct 172 -NVLPYGLAVELDL 184
>P24689 Type II secretion system protein J [Dickeya chrysanthemi]
Length=206
Score = 57.0 bits (136), Expect = 7e-14
Identities = 48/166 (29%), Positives = 83/166 (50%), Gaps = 14/166 (8%)
Query 6 GFTLIELLVSIAIFAILSALGWKIFDYLIKIKDRNQVYEQQLFELQDAYQLLLRDSLQII 65
GFTL+E+++++AIFA LS ++ + +++ + + E +L ELQ + L+ RD QI+
Sbjct 7 GFTLLEVMLALAIFAALSLAASQVMNGVMRNDEASARKEARLAELQRGFSLMERDFSQIV 66
Query 66 PNTANINGQL-----RPALSLSENILQFSRGGVSDPLKEGLAP---YERIEYRYVASEKV 117
P + +L R LS ++ + F R G +PL G+ P +R+ YR
Sbjct 67 PRRSQ-GYELGFYAERYQLSSADWAVSFIRNGWLNPL--GIMPRSELQRVGYRLRGD--T 121
Query 118 VYRLKYKNLNVVSSEQPVSSVVLKNVDQFQIAVLNPQ-LSTQWPDP 162
+ RL Y + +S+++P VL V F + +W DP
Sbjct 122 LERLFYDSSEPLSTQEPAVRPVLTGVTGFVLRFFGKDGWQERWDDP 167
>P31589 Type II secretion system protein J [Pectobacterium carotovorum
subsp. carotovorum]
Length=216
Score = 53.9 bits (128), Expect = 1e-12
Identities = 47/189 (25%), Positives = 91/189 (48%), Gaps = 16/189 (8%)
Query 2 KRHLGFTLIELLVSIAIFAILSALGWKIFDYLIKIKDRNQVYEQQLFELQDAYQLLLRDS 61
+R GFTL+E++++IAIFA LS +++ + +++ + +Q ++L E+Q A+ + D
Sbjct 21 ERQQGFTLLEMILAIAIFAALSLSAFQVLNGVMRNDEISQRKAERLAEIQRAFSQMDNDF 80
Query 62 LQIIPNTANINGQL----RPALSLSENILQFSRGGVSDPLKEGLAPYERIE-YRYVASEK 116
Q+I + + + R L + + F R G +P G+ P ++ Y E
Sbjct 81 SQMIARGSRGSTSMFYAGRDQLKSDDWGVSFMRNGWQNPF--GILPRSELQPVAYRLREH 138
Query 117 VVYRLKYKNLNVVSSEQPVSSVVLKNVDQFQIAVLNPQLSTQWPDPQIDLTQSQNLRILP 176
+ RL + + + P +L VD F++ + + W D + TQ LP
Sbjct 139 TLERLTHVTPDPIEGLDPGVKPLLTQVDGFRLRFFS---NKTWRDRWDNSTQ------LP 189
Query 177 RGLKVNLNI 185
+G++V L +
Sbjct 190 QGIEVVLTL 198
>A0A4C3GMC1 Type II secretion system protein J [Escherichia coli]
Length=199
Score = 52.8 bits (125), Expect = 2e-12
Identities = 36/106 (34%), Positives = 56/106 (53%), Gaps = 9/106 (8%)
Query 1 MKR-HLGFTLIELLVSIAIFAILSALGWKIFDYLIKIKDRNQVYEQQLFELQDAYQLLLR 59
MKR GFTL+E+LV+IAIFA L+ + ++ + + ++ ++Q+L +Q L
Sbjct 1 MKRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADHDQKLNLMQQTMSFLTH 60
Query 60 DSLQIIPN-TANINGQLRPALSLSENIL-------QFSRGGVSDPL 97
D Q++P GQ PAL +L +F RGGV +PL
Sbjct 61 DLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGVVNPL 106
>P45761 Type II secretion system protein J [Escherichia coli (strain
K12)]
Length=195
Score = 47.8 bits (112), Expect = 1e-10
Identities = 24/65 (37%), Positives = 40/65 (62%), Gaps = 0/65 (0%)
Query 1 MKRHLGFTLIELLVSIAIFAILSALGWKIFDYLIKIKDRNQVYEQQLFELQDAYQLLLRD 60
+ R GFTL+E++ ++AIF++LS L + IF ++ R+Q QQ +LQ +L D
Sbjct 2 INRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKEIQQFNQLQRTITILDND 61
Query 61 SLQII 65
LQ++
Sbjct 62 LLQLV 66
Score = 18.1 bits (35), Expect = 1.1
Identities = 12/37 (32%), Positives = 19/37 (51%), Gaps = 2/37 (5%)
Query 138 VVLKNVDQFQIAVLNPQ--LSTQWPDPQIDLTQSQNL 172
V +N +I VL + +TQ DP L+++Q L
Sbjct 66 VARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL 102
>P45776 Type II secretion system protein J [Vibrio cholerae serotype
O1 (strain ATCC 39315 / El Tor Inaba N16961)]
Length=221
Score = 40.8 bits (94), Expect = 3e-08
Identities = 53/197 (27%), Positives = 87/197 (44%), Gaps = 35/197 (18%)
Query 6 GFTLIELLVSIAIFAILSALGWKIFDYLIKIKDRNQVYEQQLFELQDAYQLLLRDSLQII 65
GFTLIE+LV+IAIFA LS +++ + + + + + +L ELQ A ++ D Q+
Sbjct 15 GFTLIEVLVAIAIFASLSVGAYQVLNQVQRSNEISAERTARLAELQRAMVIMDADFRQMA 74
Query 66 PNTANINGQLRPALSLSENILQFSRGGVSDPLKEGLA--------PYERIEYRYVASEKV 117
+G+ + SE ILQ+ + + D + GL P ++ VA KV
Sbjct 75 LRQFRTDGE-----APSEQILQW-KESLLDSDQHGLLFVRLGWHNPQQQFPRGEVA--KV 126
Query 118 VYRLKYKNLNVV--------SSEQPVSSVVLKNVDQFQIA-VLNPQLSTQWPDPQIDLTQ 168
YRL L V + +Q + S +L V+ + + L + S +W
Sbjct 127 GYRLFENRLERVWWRYPDTPAGQQGLISPLLTGVEDWAVQFYLQGEWSKEWVP------- 179
Query 169 SQNLRILPRGLKVNLNI 185
LP +KV L +
Sbjct 180 ---TNALPEAVKVTLRL 193
>P36647 Prepilin peptidase-dependent protein D [Escherichia coli
(strain K12)]
Length=146
Score = 34.7 bits (78), Expect = 3e-06
Identities = 17/26 (65%), Positives = 20/26 (77%), Gaps = 0/26 (0%)
Query 1 MKRHLGFTLIELLVSIAIFAILSALG 26
M + GFTLIEL+V I I AILSA+G
Sbjct 1 MDKQRGFTLIELMVVIGIIAILSAIG 26
>P35646 Fimbrial protein EcpB [Eikenella corrodens]
Length=159
Score = 34.3 bits (77), Expect = 4e-06
Identities = 18/45 (40%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
Query 1 MKRHLGFTLIELLVSIAIFAILSALGWKIF-DYLIKIKDRNQVYE 44
M + GFTLIEL++ IAI IL+A+ ++ D++ K + YE
Sbjct 1 MYKQKGFTLIELMIVIAIIGILAAIALPLYQDHMAKAQINRVFYE 45
Lambda K H a alpha
0.323 0.139 0.402 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 305472
Database: b83636c35f139c6d7221db81341b55ab.SwissProt.fasta
Posted date: May 9, 2024 4:24 AM
Number of letters in database: 2,183
Number of sequences in database: 11
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
