BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 6f3bd80837d1dd3a71087bbc228c914b.SwissProt.fasta
14 sequences; 3,399 total letters
Query= ACIAD2376
Length=159
Score E
Sequences producing significant alignments: (Bits) Value
P0AF69 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE ... 135 6e-45
P0AF68 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE ... 135 6e-45
P0AF67 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE ... 135 6e-45
P44492 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE ... 127 2e-41
Q92JQ4 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE ... 77.0 7e-22
O05515 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE ... 76.3 1e-21
Q4UNJ0 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE ... 75.5 3e-21
Q1RGZ7 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE ... 72.8 2e-20
Q68XZ1 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE ... 71.6 4e-20
Q9ZED0 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE ... 70.5 1e-19
O67011 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE ... 64.3 2e-17
P74415 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE ... 64.7 2e-17
O52749 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE ... 64.3 4e-17
Q08409 ATP-dependent permease AUS1 [Saccharomyces cerevisiae (str... 33.5 1e-05
>P0AF69 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
[Shigella flexneri]
Length=153
Score = 135 bits (340), Expect = 6e-45
Identities = 71/123 (58%), Positives = 87/123 (71%), Gaps = 2/123 (2%)
Query 10 LTLNDEQDTQNLAKTLSK-CFTEGVIYLIGDLGAGKTTFTRYLLQALGHQGAVKSPTYTL 68
+ L DEQ T +L + ++K C VIYL GDLGAGKTTF+R LQALGHQG VKSPTYTL
Sbjct 6 IPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTFSRGFLQALGHQGNVKSPTYTL 65
Query 69 VEPYKIKQKDIFHFDLYRLNDPYELELMGIRDYLDVPNALFLFEWPSKGGDDIPDANIVI 128
VEPY + ++HFDLYRL DP ELE MGIRDY +A+ L EWP +G +PD ++ I
Sbjct 66 VEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYF-ANDAICLVEWPQQGTGVLPDPDVEI 124
Query 129 QIE 131
I+
Sbjct 125 HID 127
>P0AF68 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
[Escherichia coli O157:H7]
Length=153
Score = 135 bits (340), Expect = 6e-45
Identities = 71/123 (58%), Positives = 87/123 (71%), Gaps = 2/123 (2%)
Query 10 LTLNDEQDTQNLAKTLSK-CFTEGVIYLIGDLGAGKTTFTRYLLQALGHQGAVKSPTYTL 68
+ L DEQ T +L + ++K C VIYL GDLGAGKTTF+R LQALGHQG VKSPTYTL
Sbjct 6 IPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTFSRGFLQALGHQGNVKSPTYTL 65
Query 69 VEPYKIKQKDIFHFDLYRLNDPYELELMGIRDYLDVPNALFLFEWPSKGGDDIPDANIVI 128
VEPY + ++HFDLYRL DP ELE MGIRDY +A+ L EWP +G +PD ++ I
Sbjct 66 VEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYF-ANDAICLVEWPQQGTGVLPDPDVEI 124
Query 129 QIE 131
I+
Sbjct 125 HID 127
>P0AF67 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
[Escherichia coli (strain K12)]
Length=153
Score = 135 bits (340), Expect = 6e-45
Identities = 71/123 (58%), Positives = 87/123 (71%), Gaps = 2/123 (2%)
Query 10 LTLNDEQDTQNLAKTLSK-CFTEGVIYLIGDLGAGKTTFTRYLLQALGHQGAVKSPTYTL 68
+ L DEQ T +L + ++K C VIYL GDLGAGKTTF+R LQALGHQG VKSPTYTL
Sbjct 6 IPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTFSRGFLQALGHQGNVKSPTYTL 65
Query 69 VEPYKIKQKDIFHFDLYRLNDPYELELMGIRDYLDVPNALFLFEWPSKGGDDIPDANIVI 128
VEPY + ++HFDLYRL DP ELE MGIRDY +A+ L EWP +G +PD ++ I
Sbjct 66 VEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYF-ANDAICLVEWPQQGTGVLPDPDVEI 124
Query 129 QIE 131
I+
Sbjct 125 HID 127
>P44492 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
[Haemophilus influenzae (strain ATCC 51907 / DSM 11121 /
KW20 / Rd)]
Length=158
Score = 127 bits (318), Expect = 2e-41
Identities = 67/119 (56%), Positives = 83/119 (70%), Gaps = 3/119 (3%)
Query 19 QNLAKTLSKCFTEGVI--YLIGDLGAGKTTFTRYLLQALGHQGAVKSPTYTLVEPYKIKQ 76
+ A+ L K TE I YL GDLGAGKTT TR +LQ +GHQG VKSPTYTLVE Y I
Sbjct 19 KKFAEILLKLHTEKAIMVYLNGDLGAGKTTLTRGMLQGIGHQGNVKSPTYTLVEEYNIAG 78
Query 77 KDIFHFDLYRLNDPYELELMGIRDYLDVPNALFLFEWPSKGGDDIPDANIVIQIEKSED 135
K I+HFDLYRL DP ELE MGIRDY + +++ L EW KG +P+A+I++ I+ +D
Sbjct 79 KMIYHFDLYRLADPEELEFMGIRDYFNT-DSICLIEWSEKGQGILPEADILVNIDYYDD 136
>Q92JQ4 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
[Rickettsia conorii (strain ATCC VR-613 / Malish 7)]
Length=175
Score = 77.0 bits (188), Expect = 7e-22
Identities = 43/105 (41%), Positives = 63/105 (60%), Gaps = 4/105 (4%)
Query 11 TLNDEQDTQNLAKTLSKCFTEGVIYLI-GDLGAGKTTFTRYLLQAL-GHQGAVKSPTYTL 68
TLN E++T+ LAK L++ I L+ GDLGAGKT F R +++ G ++ SPT+ L
Sbjct 3 TLNSEEETKKLAKLLAQSLKPNDIVLLNGDLGAGKTFFCREIIKYFCGENTSIISPTFNL 62
Query 69 VEPYKIKQKDIFHFDLYRLNDPYELELMGIRDYLDVPNALFLFEW 113
++ YK I+H+DLYRL P E+ +G + L+ L L EW
Sbjct 63 LQTYKASNFTIYHYDLYRLKSPEEIYELGFEEALN--GNLILIEW 105
>O05515 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
[Bacillus subtilis (strain 168)]
Length=158
Score = 76.3 bits (186), Expect = 1e-21
Identities = 54/149 (36%), Positives = 83/149 (56%), Gaps = 7/149 (5%)
Query 11 TLNDEQDTQNLAKTLSKCFTEG-VIYLIGDLGAGKTTFTRYLLQALGHQGAVKSPTYTLV 69
T+N E+ T+ +AK + G V+ L GDLGAGKTTFT+ + LG V SPT+T++
Sbjct 8 TVNPEE-TKAIAKLTAAFAKPGDVLTLEGDLGAGKTTFTKGFAEGLGITRIVNSPTFTII 66
Query 70 EPYKIKQKDIFHFDLYRLNDPYELELMGIRDYLDVPNALFLFEWPSKGGDDIPDANIVIQ 129
+ Y ++H D+YR+ D E E +G+ +Y + L EW + +P + I
Sbjct 67 KEYNDGVLPLYHMDVYRMED--ESEDLGLDEYFH-GQGVCLVEWAHLIEEQLPQERLQIV 123
Query 130 IEKSEDDVQRFITFSLENAALYECLKEKL 158
I+++ DD +R ITF+ YE L E+L
Sbjct 124 IKRAGDD-EREITFTAVGNR-YEMLCEEL 150
>Q4UNJ0 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
[Rickettsia felis (strain ATCC VR-1525 / URRWXCal2)]
Length=171
Score = 75.5 bits (184), Expect = 3e-21
Identities = 43/106 (41%), Positives = 63/106 (59%), Gaps = 4/106 (4%)
Query 10 LTLNDEQDTQNLAKTLSKCFTEGVIYLI-GDLGAGKTTFTRYLLQAL-GHQGAVKSPTYT 67
L LN+E++T+ LAK L++ I L+ GDLGAGKT F R +++ G + SPT+
Sbjct 2 LILNNEEETKKLAKLLAQSLKPNDIVLLNGDLGAGKTFFCREIIKHFCGENTNIISPTFN 61
Query 68 LVEPYKIKQKDIFHFDLYRLNDPYELELMGIRDYLDVPNALFLFEW 113
L++ YK I+H+DLYRL P E+ +G + L+ L L EW
Sbjct 62 LLQTYKTSNFTIYHYDLYRLKSPEEIYELGFEEALN--GNLILIEW 105
>Q1RGZ7 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
[Rickettsia bellii (strain RML369-C)]
Length=144
Score = 72.8 bits (177), Expect = 2e-20
Identities = 43/127 (34%), Positives = 67/127 (53%), Gaps = 4/127 (3%)
Query 12 LNDEQDTQNLAKTLSKCFT-EGVIYLIGDLGAGKTTFTRYLLQAL-GHQGAVKSPTYTLV 69
LN E++T+N AK + ++ L GDLG GKT F R +++ G ++ SPT+ L+
Sbjct 10 LNSEEETKNFAKAFAATLKPNNIVLLNGDLGVGKTFFCREIIKYFCGENTSIISPTFNLL 69
Query 70 EPYKIKQKDIFHFDLYRLNDPYELELMGIRDYLDVPNALFLFEWPSKGGDDIPDANIVIQ 129
+ YK I+H DLYRL P E+ +G+ + L L L EW +P I +
Sbjct 70 QTYKTPHFTIYHCDLYRLKSPEEIYELGLEEALS--GNLTLIEWSEIIKHLLPTPLIEVN 127
Query 130 IEKSEDD 136
++ +DD
Sbjct 128 LKLLDDD 134
>Q68XZ1 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
[Rickettsia typhi (strain ATCC VR-144 / Wilmington)]
Length=144
Score = 71.6 bits (174), Expect = 4e-20
Identities = 40/105 (38%), Positives = 62/105 (59%), Gaps = 4/105 (4%)
Query 11 TLNDEQDTQNLAKTLSKCFTEGVIYLI-GDLGAGKTTFTRYLLQAL-GHQGAVKSPTYTL 68
TLN E++T+N AK ++ I L+ GDLGAGKT F R +++ G + SPT+ L
Sbjct 3 TLNSEKETKNFAKLFAQNLKPNDIVLLNGDLGAGKTFFCREIIKHFCGKNTNIISPTFNL 62
Query 69 VEPYKIKQKDIFHFDLYRLNDPYELELMGIRDYLDVPNALFLFEW 113
++ YK +I+H+D+YR+ P E+ +G + L+ L L EW
Sbjct 63 LQIYKTPNFNIYHYDMYRIKSPEEIYELGFEEALN--GNLILIEW 105
>Q9ZED0 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
[Rickettsia prowazekii (strain Madrid E)]
Length=144
Score = 70.5 bits (171), Expect = 1e-19
Identities = 39/105 (37%), Positives = 63/105 (60%), Gaps = 4/105 (4%)
Query 11 TLNDEQDTQNLAKTLSKCFTEGVIYLI-GDLGAGKTTFTRYLLQAL-GHQGAVKSPTYTL 68
TLN +++T+N AK ++ I L+ GDLGAGKT F R +++ G + SPT+ L
Sbjct 3 TLNSKKETKNFAKLFAQNLKPNDIVLLNGDLGAGKTFFCREIIKHFCGKNTNIISPTFNL 62
Query 69 VEPYKIKQKDIFHFDLYRLNDPYELELMGIRDYLDVPNALFLFEW 113
++ YK + +I+H+D+YR+ P E+ +G + L+ L L EW
Sbjct 63 LQIYKTPKFNIYHYDMYRIKSPEEIYELGFEEALN--GNLILIEW 105
>O67011 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
[Aquifex aeolicus (strain VF5)]
Length=133
Score = 64.3 bits (155), Expect = 2e-17
Identities = 46/139 (33%), Positives = 74/139 (53%), Gaps = 15/139 (11%)
Query 4 MQYSFRLTLNDEQDTQNLAKTLSKCFTEG-VIYLIGDLGAGKTTFTRYLLQALG--HQGA 60
M+ + L E+DT LA+ +++ VI L G LGAGKTTF + L +AL + A
Sbjct 1 MELLKEVILESEEDTYKLAEEIAQLLKGSEVICLRGTLGAGKTTFVKALAKALKVKNPSA 60
Query 61 VKSPTYTLVEPYKIKQKDIFHFDLYRLNDPYELELMGIRDYLDVPNALFLFEWPSKGGDD 120
V+SPT+TLV Y+ + + H DLYR+ D E +G + EW + D
Sbjct 61 VRSPTFTLVNEYETDKGKLIHIDLYRVPDFDYSEFIG--------EGILAVEWEER---D 109
Query 121 IPDANIVIQIEKSEDDVQR 139
P +I+++IE +++ ++
Sbjct 110 KP-CDIILEIEILDENKRK 127
>P74415 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
[Synechocystis sp. (strain PCC 6803 / Kazusa)]
Length=157
Score = 64.7 bits (156), Expect = 2e-17
Identities = 41/114 (36%), Positives = 59/114 (52%), Gaps = 5/114 (4%)
Query 7 SFRLTLNDEQDTQNLAKTLSKCFTEGVIYLI-GDLGAGKTTFTRYLLQALGHQGAVKSPT 65
S L D T + L++ G I L+ GDLGAGKT+ + L + LG G + SPT
Sbjct 5 SMEFFLPDLNATDQWGQQLAQQLPLGTIILLQGDLGAGKTSLVQGLGRGLGITGEIVSPT 64
Query 66 YTLVEPYKIKQKDIFHFDLYRLNDPYELELMGIRDYL---DVPNALFLFEWPSK 116
+T+V Y+ + ++H DLYRLN E+E + Y D P + EWP +
Sbjct 65 FTIVNEYREGKMPLYHLDLYRLN-TLEVEYLYPEQYWQGEDFPLGITAVEWPER 117
Score = 16.2 bits (30), Expect = 4.9
Identities = 8/20 (40%), Positives = 11/20 (55%), Gaps = 0/20 (0%)
Query 48 TRYLLQALGHQGAVKSPTYT 67
++YL L HQG +S T
Sbjct 123 SQYLQIQLCHQGEGRSIALT 142
>O52749 tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
[Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)]
Length=162
Score = 64.3 bits (155), Expect = 4e-17
Identities = 40/118 (34%), Positives = 63/118 (53%), Gaps = 5/118 (4%)
Query 3 TMQYSFRLTLNDEQDTQNLAKTLSKCFTEG-VIYLIGDLGAGKTTFTRYLLQALGHQGAV 61
T+ ++ L D++ T NL L + T G VI L GDLGAGKTT + L + L +
Sbjct 7 TIDQMTKIFLADKESTLNLGILLGETLTAGSVILLEGDLGAGKTTLVQGLGKGLSITEPI 66
Query 62 KSPTYTLVEPYKIKQKDIFHFDLYRLNDPYELELMGIRDY---LDVPNALFLFEWPSK 116
SPT+TL+ Y + ++H DLYRL +P E+ + + Y +++ + EW +
Sbjct 67 VSPTFTLINEYTEGRIPLYHLDLYRL-EPQEVLSLNLEIYWEGIEIIPGIVAIEWSER 123
>Q08409 ATP-dependent permease AUS1 [Saccharomyces cerevisiae
(strain ATCC 204508 / S288c)]
Length=1394
Score = 33.5 bits (75), Expect = 1e-05
Identities = 30/113 (27%), Positives = 47/113 (42%), Gaps = 19/113 (17%)
Query 12 LNDEQDTQNLAKTLSKCFTEGVIYLIGDLGAGKTTFTRYLLQALGHQGAVKSPTYTLVEP 71
+N T+ L S + G+ L+G+ GAGKTT L Q + G V L++
Sbjct 756 INYTVGTKKLINNASGFISSGLTALMGESGAGKTTLLNVLSQRV-ETGVVSGE--ILIDG 812
Query 72 YKIKQKDIF------------HFDLYRLNDPYELELM----GIRDYLDVPNAL 108
+ + +D F H DL + + E+ + G R YLD + L
Sbjct 813 HPLTDEDAFKRSIGFVQQQDLHLDLLSVKESLEISCLLRGDGDRAYLDTVSNL 865
Lambda K H a alpha
0.324 0.142 0.420 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 346907
Database: 6f3bd80837d1dd3a71087bbc228c914b.SwissProt.fasta
Posted date: May 20, 2024 8:47 AM
Number of letters in database: 3,399
Number of sequences in database: 14
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
