BLASTP 2.12.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.


Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.



Database: 28f7aa52d2b3ce899a78da5dc575519e.SwissProt.fasta
           2 sequences; 475 total letters



Query= ACIAD2576

Length=230
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

Q88KV1 tRNA 2-(methylsulfanyl)-N(6)-isopentenyladenosine(37) hydr...  205     2e-71
Q08015 tRNA 2-(methylsulfanyl)-N(6)-isopentenyladenosine(37) hydr...  167     6e-56


>Q88KV1 tRNA 2-(methylsulfanyl)-N(6)-isopentenyladenosine(37) 
hydroxylase [Pseudomonas putida (strain ATCC 47054 / DSM 6125 
/ CFBP 8728 / NCIMB 11950 / KT2440)]
Length=205

 Score = 205 bits (521),  Expect = 2e-71
 Identities = 105/210 (50%), Positives = 141/210 (67%), Gaps = 7/210 (3%)

Query  20   LMQPVIAFLGCETPQAWLDEAVNNLELLMQDHANCEKKAAGTAMNLMFRYSFFTDLQVKL  79
            L+  + AFLGC TP AW++ A+ + E L+ DH NCE KAA TA++L+ +Y+   DL   +
Sbjct  3    LIPEIDAFLGCPTPDAWIEAALADQETLLIDHKNCEFKAASTALSLIAKYNTHLDLINMM  62

Query  80   AQLVREEMLHYEQVLEFMNKRGQAWKAVSAGRYAGELRKEIRTYEPEALIDVLVVGAFVE  139
            ++L REE++H+EQVL  M +RG   + VSAGRYA  LR+ +R +EP  L+D LVVGAF+E
Sbjct  63   SRLAREELVHHEQVLRLMKRRGVPLRPVSAGRYASGLRRLVRAHEPVKLVDTLVVGAFIE  122

Query  140  ARSCERFHALAPLVDEELGRYYRYLLKSESRHFEDYLALALEVAKTAKMKNPEEDIEQRI  199
            ARSCERF AL P +DEELGR+Y  LLKSE+RH++ YL       K A     E DI + +
Sbjct  123  ARSCERFAALVPHLDEELGRFYHGLLKSEARHYQGYL-------KLAHNYGDEADIARCV  175

Query  200  QRIREVENNLISTPDDVFRFHSGVPPFKAA  229
            + +R  E  LI +PD   RFHSG+P   AA
Sbjct  176  ELVRAAEMELIQSPDQELRFHSGIPQALAA  205


>Q08015 tRNA 2-(methylsulfanyl)-N(6)-isopentenyladenosine(37) 
hydroxylase [Salmonella typhimurium (strain LT2 / SGSC1412 / 
ATCC 700720)]
Length=270

 Score = 167 bits (424),  Expect = 6e-56
 Identities = 104/265 (39%), Positives = 147/265 (55%), Gaps = 60/265 (23%)

Query  9    RLKMSNPNYDELMQPVIAFLGCETPQAWLDEAVN--NLELLMQDHANCEKKAAGTAMNLM  66
            RL+M   NY +++ PV+ FL C TPQAW+ +A +  NL LL+ DH  CE KAA TA+ L+
Sbjct  14   RLRM---NYPQILSPVLNFLHCPTPQAWIVQARDPQNLPLLLTDHLICELKAAQTALLLV  70

Query  67   FRYSF---------------------------FTDLQVKLAQ------------------  81
             +Y                             F  L  ++++                  
Sbjct  71   RKYVADKSGADALLSWLQPYEAFAFRQGPEPDFVALHKQISKSAMPQTDDPWGRQLIDRM  130

Query  82   --LVREEMLHYEQVLEFMNKRGQAWKAVSAGRYAGELRKEIRTYEPEALIDVLVVGAFVE  139
              L++EE+ H+ QV E M  R   +  ++A RYA  + K +RT+EP  LID L+ GA++E
Sbjct  131  VLLIKEELHHFWQVREVMQARNIPYVKITASRYAKGMLKAVRTHEPLTLIDKLICGAYIE  190

Query  140  ARSCERFHALAPLVDEELGRYYRYLLKSESRHFEDYLALALEVAKTAKMKNPEEDIEQRI  199
            ARSCERF ALAP +DE+L  +Y  LL+SE+RH++DYLALA +++         EDI  R+
Sbjct  191  ARSCERFAALAPWLDEDLQTFYLSLLRSEARHYQDYLALAQQIS--------AEDISARV  242

Query  200  QRIREVENNLISTPDDVFRFHSGVP  224
            +   EVE +LI +PD  FRFHSGVP
Sbjct  243  RYFGEVEADLILSPDREFRFHSGVP  267



Lambda      K        H        a         alpha
   0.324    0.137    0.403    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 84638


  Database: 28f7aa52d2b3ce899a78da5dc575519e.SwissProt.fasta
    Posted date:  May 9, 2024  1:19 PM
  Number of letters in database: 475
  Number of sequences in database:  2



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40