BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 95c7874b97dd009de9fff7853b06ff68.SwissProt.fasta
3 sequences; 729 total letters
Query= ACIAD2699
Length=288
Score E
Sequences producing significant alignments: (Bits) Value
O34360 Peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK [Bac... 59.7 4e-15
Q37979 L-alanyl-D-glutamate peptidase [Listeria phage A500] 55.8 3e-13
O34866 Putative carboxypeptidase YodJ [Bacillus subtilis (strain ... 42.0 1e-08
>O34360 Peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK
[Bacillus subtilis (strain 168)]
Length=167
Score = 59.7 bits (143), Expect = 4e-15
Identities = 37/102 (36%), Positives = 53/102 (52%), Gaps = 10/102 (10%)
Query 168 GYELVLLEGYRSPERQNLLSGNPNT------TRARGYQSYHQFGLAADVAF-KRNGKVVI 220
G ++V+ EG+RS + Q+ L T T ARG +SYH +GLA D A K++G ++
Sbjct 62 GIDVVITEGFRSFKEQDELYKQGRTKKGNIVTYARGGESYHNYGLAIDFALQKKDGSIIW 121
Query 221 SERDPWAMRGYQLYGEVAE---SVGLTWGGRWKSIQDYGHTE 259
G + EV E ++G WGG WK +DY H E
Sbjct 122 DMEYDGNQNGKSDWLEVVEIAKTLGFEWGGDWKRFKDYPHLE 163
>Q37979 L-alanyl-D-glutamate peptidase [Listeria phage A500]
Length=289
Score = 55.8 bits (133), Expect = 3e-13
Identities = 39/109 (36%), Positives = 53/109 (49%), Gaps = 12/109 (11%)
Query 158 MVFKIMKERYGYELVLLEGYRSPERQNLLSGNPNT------TRARGYQSYHQFGLAADVA 211
++ K+ KE G L + +GYRS QN L T T A+G QS H +G+A D+
Sbjct 32 VIKKMAKE--GIYLCVAQGYRSTAEQNALYAQGRTKPGAIVTNAKGGQSNHNYGVAVDLC 89
Query 212 FKRN-GKVVISERDPWAMRGYQLYGEVAESVGLTWGGRWKSIQDYGHTE 259
N GK VI E ++ ++ G WGG WKS +DY H E
Sbjct 90 LYTNDGKDVIWES---TTSRWKKVVAAMKAEGFKWGGDWKSFKDYPHFE 135
Score = 15.0 bits (27), Expect = 5.4
Identities = 15/69 (22%), Positives = 27/69 (39%), Gaps = 3/69 (4%)
Query 145 WDKMNPRYKQRLLMVFKIMKERYGYELVLLEGYRSPERQNLLSGN--PNTTRARGYQSYH 202
W+ R+K +++ K ++G + + Y E + +SG P T+ S
Sbjct 100 WESTTSRWK-KVVAAMKAEGFKWGGDWKSFKDYPHFELCDAVSGEKIPAATQNTNTNSNR 158
Query 203 QFGLAADVA 211
G D A
Sbjct 159 YEGKVIDSA 167
>O34866 Putative carboxypeptidase YodJ [Bacillus subtilis (strain
168)]
Length=273
Score = 42.0 bits (97), Expect = 1e-08
Identities = 45/166 (27%), Positives = 67/166 (40%), Gaps = 48/166 (29%)
Query 146 DKMNPRYKQR-----LLMVFKIMKERYGYELVLLEGYRSPERQNL-------LSGNPNTT 193
+K+ RY ++ L +F K+ GYEL + GYRS +RQ + L G
Sbjct 116 EKIQKRYIRKEAADALKTMFDAAKKE-GYELAAVSGYRSYDRQKVIFDNEVSLKGERKAK 174
Query 194 RARGY--QSYHQFGLAADVAFKRNGKVVISERDPWAMRGYQLYGEVAESVGLTWGGRW-- 249
A Y +S HQ GLA D++ + NG E+ E+ G T G+W
Sbjct 175 EAVAYPGESEHQTGLAMDISSRSNG------------------FELNEAFGSTADGKWVQ 216
Query 250 -------------KSIQDYGHTEYRMPGLRKTKEMAEQLIAEGQLT 282
K+ +D EY LR + A ++I + LT
Sbjct 217 DNAYKYGFIIRYPKNKEDITKYEYEPWHLRYVGKKAAKVIQDNDLT 262
Lambda K H a alpha
0.320 0.133 0.400 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 162048
Database: 95c7874b97dd009de9fff7853b06ff68.SwissProt.fasta
Posted date: May 31, 2024 4:21 AM
Number of letters in database: 729
Number of sequences in database: 3
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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