BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 066968cbdd6967b165b84a1e82ce9649.SwissProt.fasta
6 sequences; 2,521 total letters
Query= ACIAD2736
Length=264
Score E
Sequences producing significant alignments: (Bits) Value
P0AFM1 Intermembrane transport protein PqiA [Shigella flexneri] 162 6e-51
P0AFM0 Intermembrane transport protein PqiA [Escherichia coli O6:... 162 6e-51
P0AFL9 Intermembrane transport protein PqiA [Escherichia coli (st... 162 6e-51
P44287 Uncharacterized protein HI_1671 [Haemophilus influenzae (s... 137 1e-41
P0AD04 Intermembrane transport protein YebS [Escherichia coli O15... 129 1e-38
P0AD03 Intermembrane transport protein YebS [Escherichia coli (st... 129 1e-38
>P0AFM1 Intermembrane transport protein PqiA [Shigella flexneri]
Length=417
Score = 162 bits (409), Expect = 6e-51
Identities = 88/233 (38%), Positives = 138/233 (59%), Gaps = 20/233 (9%)
Query 19 DMAPVNPPKNQRYPRAVDL--SLIECHCCGLLNQVDSDHSEQAHSRHCIRCHSPLHVRKP 76
D+AP+ + P + L C CC + D C RC + +VR+
Sbjct 199 DIAPMPELRQPLKPGVTGIRQGLRSCSCCTAILPADEPV--------CPRCSTKGYVRRR 250
Query 77 RSLDRTLALVIAASILYIPANVLPMTMTDSLLGRQQDTIMSGVIYFWQNSDYLVAVVIFM 136
SL TLAL++ + +LY+PAN+LP+ +TD L + TI++GVI W Y VA VIF+
Sbjct 251 NSLQWTLALLVTSIMLYLPANILPIMVTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFL 310
Query 137 ASIFIPMLKLIILCFLLIAVR---MQESSKWHFLPEHCALLYRIVEFVGRWSMIDVFVVA 193
ASI +P LK+I + +L + ++S + H L+Y +VEFVGRWSMIDVFV+A
Sbjct 311 ASIMVPTLKMIAIAWLCWDAKGHGKRDSERMH-------LIYEVVEFVGRWSMIDVFVIA 363
Query 194 LLTALIQIQSLATILAGPGAVAFGAVVVLTMFASLSFDPRIIWDNYYKAQAKQ 246
+L+AL+++ L +I GA+ F VV++TMF++++FDPR+ WD +++ ++
Sbjct 364 VLSALVRMGGLMSIYPAMGALMFALVVIMTMFSAMTFDPRLSWDRQPESEHEE 416
Score = 45.8 bits (107), Expect = 2e-09
Identities = 45/202 (22%), Positives = 84/202 (42%), Gaps = 16/202 (8%)
Query 40 IECHCCGLLNQVDSDHSEQAHSRHCIRCHSPLHVRKPRSLDRTLALVIAASILYIPANVL 99
I C C +L V E C RC + L V R A +AA + + +N+
Sbjct 11 ILCSQCDML--VALPRLEHGQKAACPRCGTTLTVAWDAPRQRPTAYALAALFMLLLSNLF 68
Query 100 PMTMTDSLLGRQQDTIMS--GVIYFWQNSDYLVAVVIFMASIFIPMLKLIILCFLLIAVR 157
P + + T++ GV++ + DY F+ +F+ ++ L +L+ V
Sbjct 69 PFVNMNVAGVTSEITLLEIPGVLF---SEDYASLGTFFL--LFVQLVPAFCLITILLLVN 123
Query 158 MQESSKWHFLPEHCA-LLYRIVEFVGRWSMIDVFVVALLTALIQIQSLATILAGPGAVAF 216
E LP L R++ + W M ++F+ +L + +++ + +I G + +
Sbjct 124 RAE------LPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFVKLMAYGSIGVGSSFLPW 177
Query 217 GAVVVLTMFASLSFDPRIIWDN 238
VL + A D R +WD+
Sbjct 178 CLFCVLQLRAFQCVDRRWLWDD 199
>P0AFM0 Intermembrane transport protein PqiA [Escherichia coli
O6:H1 (strain CFT073 / ATCC 700928 / UPEC)]
Length=417
Score = 162 bits (409), Expect = 6e-51
Identities = 88/233 (38%), Positives = 138/233 (59%), Gaps = 20/233 (9%)
Query 19 DMAPVNPPKNQRYPRAVDL--SLIECHCCGLLNQVDSDHSEQAHSRHCIRCHSPLHVRKP 76
D+AP+ + P + L C CC + D C RC + +VR+
Sbjct 199 DIAPMPELRQPLKPGVTGIRQGLRSCSCCTAILPADEPV--------CPRCSTKGYVRRR 250
Query 77 RSLDRTLALVIAASILYIPANVLPMTMTDSLLGRQQDTIMSGVIYFWQNSDYLVAVVIFM 136
SL TLAL++ + +LY+PAN+LP+ +TD L + TI++GVI W Y VA VIF+
Sbjct 251 NSLQWTLALLVTSIMLYLPANILPIMVTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFL 310
Query 137 ASIFIPMLKLIILCFLLIAVR---MQESSKWHFLPEHCALLYRIVEFVGRWSMIDVFVVA 193
ASI +P LK+I + +L + ++S + H L+Y +VEFVGRWSMIDVFV+A
Sbjct 311 ASIMVPTLKMIAIAWLCWDAKGHGKRDSERMH-------LIYEVVEFVGRWSMIDVFVIA 363
Query 194 LLTALIQIQSLATILAGPGAVAFGAVVVLTMFASLSFDPRIIWDNYYKAQAKQ 246
+L+AL+++ L +I GA+ F VV++TMF++++FDPR+ WD +++ ++
Sbjct 364 VLSALVRMGGLMSIYPAMGALMFALVVIMTMFSAMTFDPRLSWDRQPESEHEE 416
Score = 45.8 bits (107), Expect = 2e-09
Identities = 45/202 (22%), Positives = 84/202 (42%), Gaps = 16/202 (8%)
Query 40 IECHCCGLLNQVDSDHSEQAHSRHCIRCHSPLHVRKPRSLDRTLALVIAASILYIPANVL 99
I C C +L V E C RC + L V R A +AA + + +N+
Sbjct 11 ILCSQCDML--VALPRLEHGQKAACPRCGTTLTVAWDAPRQRPTAYALAALFMLLLSNLF 68
Query 100 PMTMTDSLLGRQQDTIMS--GVIYFWQNSDYLVAVVIFMASIFIPMLKLIILCFLLIAVR 157
P + + T++ GV++ + DY F+ +F+ ++ L +L+ V
Sbjct 69 PFVNMNVAGVTSEITLLEIPGVLF---SEDYASLGTFFL--LFVQLVPAFCLITILLLVN 123
Query 158 MQESSKWHFLPEHCA-LLYRIVEFVGRWSMIDVFVVALLTALIQIQSLATILAGPGAVAF 216
E LP L R++ + W M ++F+ +L + +++ + +I G + +
Sbjct 124 RAE------LPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFVKLMAYGSIGVGSSFLPW 177
Query 217 GAVVVLTMFASLSFDPRIIWDN 238
VL + A D R +WD+
Sbjct 178 CLFCVLQLRAFQCVDRRWLWDD 199
>P0AFL9 Intermembrane transport protein PqiA [Escherichia coli
(strain K12)]
Length=417
Score = 162 bits (409), Expect = 6e-51
Identities = 88/233 (38%), Positives = 138/233 (59%), Gaps = 20/233 (9%)
Query 19 DMAPVNPPKNQRYPRAVDL--SLIECHCCGLLNQVDSDHSEQAHSRHCIRCHSPLHVRKP 76
D+AP+ + P + L C CC + D C RC + +VR+
Sbjct 199 DIAPMPELRQPLKPGVTGIRQGLRSCSCCTAILPADEPV--------CPRCSTKGYVRRR 250
Query 77 RSLDRTLALVIAASILYIPANVLPMTMTDSLLGRQQDTIMSGVIYFWQNSDYLVAVVIFM 136
SL TLAL++ + +LY+PAN+LP+ +TD L + TI++GVI W Y VA VIF+
Sbjct 251 NSLQWTLALLVTSIMLYLPANILPIMVTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFL 310
Query 137 ASIFIPMLKLIILCFLLIAVR---MQESSKWHFLPEHCALLYRIVEFVGRWSMIDVFVVA 193
ASI +P LK+I + +L + ++S + H L+Y +VEFVGRWSMIDVFV+A
Sbjct 311 ASIMVPTLKMIAIAWLCWDAKGHGKRDSERMH-------LIYEVVEFVGRWSMIDVFVIA 363
Query 194 LLTALIQIQSLATILAGPGAVAFGAVVVLTMFASLSFDPRIIWDNYYKAQAKQ 246
+L+AL+++ L +I GA+ F VV++TMF++++FDPR+ WD +++ ++
Sbjct 364 VLSALVRMGGLMSIYPAMGALMFALVVIMTMFSAMTFDPRLSWDRQPESEHEE 416
Score = 45.8 bits (107), Expect = 2e-09
Identities = 45/202 (22%), Positives = 84/202 (42%), Gaps = 16/202 (8%)
Query 40 IECHCCGLLNQVDSDHSEQAHSRHCIRCHSPLHVRKPRSLDRTLALVIAASILYIPANVL 99
I C C +L V E C RC + L V R A +AA + + +N+
Sbjct 11 ILCSQCDML--VALPRLEHGQKAACPRCGTTLTVAWDAPRQRPTAYALAALFMLLLSNLF 68
Query 100 PMTMTDSLLGRQQDTIMS--GVIYFWQNSDYLVAVVIFMASIFIPMLKLIILCFLLIAVR 157
P + + T++ GV++ + DY F+ +F+ ++ L +L+ V
Sbjct 69 PFVNMNVAGVTSEITLLEIPGVLF---SEDYASLGTFFL--LFVQLVPAFCLITILLLVN 123
Query 158 MQESSKWHFLPEHCA-LLYRIVEFVGRWSMIDVFVVALLTALIQIQSLATILAGPGAVAF 216
E LP L R++ + W M ++F+ +L + +++ + +I G + +
Sbjct 124 RAE------LPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFVKLMAYGSIGVGSSFLPW 177
Query 217 GAVVVLTMFASLSFDPRIIWDN 238
VL + A D R +WD+
Sbjct 178 CLFCVLQLRAFQCVDRRWLWDD 199
>P44287 Uncharacterized protein HI_1671 [Haemophilus influenzae
(strain ATCC 51907 / DSM 11121 / KW20 / Rd)]
Length=416
Score = 137 bits (345), Expect = 1e-41
Identities = 77/200 (39%), Positives = 110/200 (55%), Gaps = 11/200 (6%)
Query 42 CHCCGLLNQVDSDHSEQAHSRHCIRCHSPLHVRKPRSLDRTLALVIAASILYIPANVLPM 101
CH + DH ++ C RC SPL+ L T A +IA I+ PAN+LP+
Sbjct 222 CHYTFTQKSIHYDHQQKIC---CPRCQSPLNTSDKLKLQATWATLIAGIIMLFPANLLPI 278
Query 102 TMTDSLLGRQQDTIMSGVIYFWQNSDYLVAVVIFMASIFIPMLKLIILCFLLIAV--RMQ 159
+ +DT++SGVI F ++ Y VA V+F ASIF+P+ K+ I+ +LL V + Q
Sbjct 279 SGIYLTGALSEDTLISGVISFVKSGSYFVAFVVFFASIFVPISKIFIMLYLLACVHFKWQ 338
Query 160 ESSKWHFLPEHCALLYRIVEFVGRWSMIDVFVVALLTALIQIQSLATILAGPGAVAFGAV 219
S KW L +V FVGRWSM+D+FV+AL+ +L+ + GPGA FGA
Sbjct 339 HSIKWQMR------LLHLVHFVGRWSMLDLFVLALMMSLVTRGEIINFTVGPGAFYFGAA 392
Query 220 VVLTMFASLSFDPRIIWDNY 239
V TM ++ FD ++IW Y
Sbjct 393 VFCTMLSTSQFDSKLIWKIY 412
Score = 47.8 bits (112), Expect = 6e-10
Identities = 46/205 (22%), Positives = 91/205 (44%), Gaps = 16/205 (8%)
Query 39 LIECHCCGLLNQVDS--DHSEQAHSRHCIRCHSPLHVRKPRSLDRTLALVIAASILYIPA 96
+++C C V S +EQA C RCH L SL R A++ + ++ +P
Sbjct 13 ILKCTECDACINVPSILQSNEQAE---CPRCHHLLASGTRWSLHRC-AMIALSILILMPF 68
Query 97 NVLPMTMTDSLLGRQQD-TIMSGVIYFWQNSDYLVAVVIFMASIFIPMLKLIILCFLLIA 155
++ ++ LLG + D +I G+ A +IF+ ++ +P+ +++ L +A
Sbjct 69 SLNYPLLSLHLLGIKIDASIWDGIWKMAVGGYEYTAFMIFICAVVMPITFALLVIMLWLA 128
Query 156 VRMQESSKWHFLPEHCALLYRIVEFVGRWSMIDVFVVALLTALIQIQSLATILAGPGAVA 215
Q + LL+ + ++ W M DV++VAL + +++ AT+ +
Sbjct 129 KIFQ-------IKPRSVLLF--LGYIKAWVMFDVYLVALGVTIFKVREYATLEINIYLIP 179
Query 216 FGAVVVLTMFASLSFDPRIIWDNYY 240
F +LT + + +W +Y
Sbjct 180 FIFTALLTTLLFIKLNLSALWQEFY 204
>P0AD04 Intermembrane transport protein YebS [Escherichia coli
O157:H7]
Length=427
Score = 129 bits (325), Expect = 1e-38
Identities = 69/187 (37%), Positives = 115/187 (61%), Gaps = 9/187 (5%)
Query 64 CIRCHSPLHVRKPRSLDRTLALVIAASILYIPANVLPMTMTDSLLGRQQDTIMSGVIYFW 123
C RCH PL +R+ SL + A ++A+ +L +PAN+LP+++ GRQ+DTI+SG++
Sbjct 248 CPRCHIPLRLRRRHSLQKCWAALLASIVLLLPANLLPISIIYLNGGRQEDTILSGIMSL- 306
Query 124 QNSDYLVAVVIFMASIFIPMLKLIILCFLLIAV--RMQESSKWHFLPEHCALLYRIVEFV 181
+S+ VA ++F+ASI +P K+I++ LL+++ + Q+ + L L R+V ++
Sbjct 307 ASSNIAVAGIVFIASILVPFTKVIVMFTLLLSIHFKCQQGLRTRIL------LLRMVTWI 360
Query 182 GRWSMIDVFVVALLTALIQIQSLATILAGPGAVAFGAVVVLTMFASLSFDPRIIWDNYYK 241
GRWSM+D+FV++L +LI + GP A FGA V+LT+ A D R++WD +
Sbjct 361 GRWSMLDLFVISLTMSLINRDQILAFTMGPAAFYFGAAVILTILAVEWLDSRLLWDAHES 420
Query 242 AQAKQHD 248
A+ D
Sbjct 421 GNARFDD 427
Score = 61.6 bits (148), Expect = 1e-14
Identities = 47/199 (24%), Positives = 88/199 (44%), Gaps = 11/199 (6%)
Query 42 CHCCGLLNQVDSDHSEQAHSRHCIRCHSPLHVRKPRSLDRTLALVIAASILYIPANVLPM 101
C C +L + +S Q S +C RC + + + SL R A+ +L A P+
Sbjct 31 CPQCDMLFSLPEINSHQ--SAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPL 88
Query 102 TMTDSLLGRQQDTIMSGVIYFWQNSDYLVAVVIFMASIFIPMLKLIILCFLLIAVRMQES 161
L R +M G+ + D + ++F I P++ + + +L R+ +
Sbjct 89 LHIWLLGIRIDANVMQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMN 148
Query 162 SKWHFLPEHCALLYRIVEFVGRWSMIDVFVVALLTALIQIQSLATILAGPGAVAFGAVVV 221
+ L ++E + W M+D+++V + A I++Q A I AG G +F A+V+
Sbjct 149 LRPVLL---------MLERLKEWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVI 199
Query 222 LTMFASLSFDPRIIWDNYY 240
LT + +W+ +Y
Sbjct 200 LTTVTLSHLNVEELWERFY 218
>P0AD03 Intermembrane transport protein YebS [Escherichia coli
(strain K12)]
Length=427
Score = 129 bits (325), Expect = 1e-38
Identities = 69/187 (37%), Positives = 115/187 (61%), Gaps = 9/187 (5%)
Query 64 CIRCHSPLHVRKPRSLDRTLALVIAASILYIPANVLPMTMTDSLLGRQQDTIMSGVIYFW 123
C RCH PL +R+ SL + A ++A+ +L +PAN+LP+++ GRQ+DTI+SG++
Sbjct 248 CPRCHIPLRLRRRHSLQKCWAALLASIVLLLPANLLPISIIYLNGGRQEDTILSGIMSL- 306
Query 124 QNSDYLVAVVIFMASIFIPMLKLIILCFLLIAV--RMQESSKWHFLPEHCALLYRIVEFV 181
+S+ VA ++F+ASI +P K+I++ LL+++ + Q+ + L L R+V ++
Sbjct 307 ASSNIAVAGIVFIASILVPFTKVIVMFTLLLSIHFKCQQGLRTRIL------LLRMVTWI 360
Query 182 GRWSMIDVFVVALLTALIQIQSLATILAGPGAVAFGAVVVLTMFASLSFDPRIIWDNYYK 241
GRWSM+D+FV++L +LI + GP A FGA V+LT+ A D R++WD +
Sbjct 361 GRWSMLDLFVISLTMSLINRDQILAFTMGPAAFYFGAAVILTILAVEWLDSRLLWDAHES 420
Query 242 AQAKQHD 248
A+ D
Sbjct 421 GNARFDD 427
Score = 61.6 bits (148), Expect = 1e-14
Identities = 47/199 (24%), Positives = 88/199 (44%), Gaps = 11/199 (6%)
Query 42 CHCCGLLNQVDSDHSEQAHSRHCIRCHSPLHVRKPRSLDRTLALVIAASILYIPANVLPM 101
C C +L + +S Q S +C RC + + + SL R A+ +L A P+
Sbjct 31 CPQCDMLFSLPEINSHQ--SAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPL 88
Query 102 TMTDSLLGRQQDTIMSGVIYFWQNSDYLVAVVIFMASIFIPMLKLIILCFLLIAVRMQES 161
L R +M G+ + D + ++F I P++ + + +L R+ +
Sbjct 89 LHIWLLGIRIDANVMQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMN 148
Query 162 SKWHFLPEHCALLYRIVEFVGRWSMIDVFVVALLTALIQIQSLATILAGPGAVAFGAVVV 221
+ L ++E + W M+D+++V + A I++Q A I AG G +F A+V+
Sbjct 149 LRPVLL---------MLERLKEWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVI 199
Query 222 LTMFASLSFDPRIIWDNYY 240
LT + +W+ +Y
Sbjct 200 LTTVTLSHLNVEELWERFY 218
Lambda K H a alpha
0.329 0.138 0.432 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 510944
Database: 066968cbdd6967b165b84a1e82ce9649.SwissProt.fasta
Posted date: May 9, 2024 4:56 PM
Number of letters in database: 2,521
Number of sequences in database: 6
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
