BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 76c61b692aee54fa6d4e0157ef6a879e.SwissProt.fasta
10 sequences; 2,252 total letters
Query= ACIAD2739
Length=227
Score E
Sequences producing significant alignments: (Bits) Value
Q5AF03 Glyoxalase 3 [Candida albicans (strain SC5314 / ATCC MYA-2... 104 3e-31
Q04432 Glutathione-independent glyoxalase HSP31 [Saccharomyces ce... 92.4 1e-26
O74914 Glutathione-independent glyoxalase hsp3101 [Schizosaccharo... 84.3 2e-23
Q10092 Probable glutathione-independent glyoxalase hsp3104 [Schiz... 75.1 3e-20
O43084 Probable glutathione-independent glyoxalase hsp3103 [Schiz... 73.6 2e-19
Q08914 Probable glutathione-independent glyoxalase HSP33 [Sacchar... 71.2 1e-18
Q08992 Probable glutathione-independent glyoxalase HSP32 [Sacchar... 70.9 1e-18
Q04902 Probable glutathione-independent glyoxalase SNO4 [Saccharo... 67.8 2e-17
P80876 General stress protein 18 [Bacillus subtilis (strain 168)] 53.5 1e-12
O06006 Putative cysteine protease YraA [Bacillus subtilis (strain... 48.5 6e-11
>Q5AF03 Glyoxalase 3 [Candida albicans (strain SC5314 / ATCC MYA-2876)]
Length=236
Score = 104 bits (260), Expect = 3e-31
Identities = 70/231 (30%), Positives = 117/231 (51%), Gaps = 10/231 (4%)
Query 1 MKILMVLTSHDQ-LGNTGHKTGFWLEEFTSPYYVFKDAGADITLASPKGGQPPLDPKSND 59
+K+L+ LTS+++ + G KTG ++ E P+ VF+ G +I LAS + G D S
Sbjct 2 VKVLLALTSYNETFYSDGKKTGVFVVEALHPFEVFRKKGYEIQLAS-ETGTFGWDDHSVV 60
Query 60 PDAQTDSTRRFEQDKQAQ--QALANTVLLDQVQAEDFDAVFYPGGHGPLWDLTNDKKSIE 117
PD + + ++ AL N + D+D F GHG L+D N K +
Sbjct 61 PDFLNGEDKEIFDNVNSEFNVALKNLKKASDLDPNDYDIFFGSAGHGTLFDYPNAKDLQK 120
Query 118 LIEQITAMDKPLGLVCHAPAVLKNVK-TAAGDSFVKGKHVTGFSNSEEAAVQLTDVVP-- 174
+ + + VCH PA+ +N+ G+ +KGK +TGF++ E + +TD++
Sbjct 121 IATTVYDKGGVVSAVCHGPAIFENLNDPKTGEPLIKGKKITGFTDIGEDILGVTDIMKKG 180
Query 175 --FLIEDEFKAQGAHY-EKGADWQSFTVREGQLITGQNPASSEAVAEHLLA 222
I+ + +GA Y E W +FTV +G+++TG NP S+ AE ++A
Sbjct 181 NLLTIKQVAEKEGATYIEPEGPWDNFTVTDGRIVTGVNPQSAVKTAEDVIA 231
>Q04432 Glutathione-independent glyoxalase HSP31 [Saccharomyces
cerevisiae (strain ATCC 204508 / S288c)]
Length=237
Score = 92.4 bits (228), Expect = 1e-26
Identities = 66/233 (28%), Positives = 109/233 (47%), Gaps = 10/233 (4%)
Query 2 KILMVLTSH-DQLGNTGHKTGFWLEEFTSPYYVFKDAGADITLASPKGGQPPLDPKSNDP 60
K+L+ LTS+ D + G KTG ++ E P+ F+ G ++ S + G+ D S
Sbjct 5 KVLLALTSYNDVFYSDGAKTGVFVVEALHPFNTFRKEGFEVDFVS-ETGKFGWDEHSLAK 63
Query 61 DAQT--DSTRRFEQDKQAQQALANTVLLDQVQAEDFDAVFYPGGHGPLWDLTNDKKSIEL 118
D D T +D + LA +V A+D+ F GHG L+D K ++
Sbjct 64 DFLNGQDETDFKNKDSDFNKTLAKIKTPKEVNADDYQIFFASAGHGTLFDYPKAKDLQDI 123
Query 119 IEQITAMDKPLGLVCHAPAVLKNV-KTAAGDSFVKGKHVTGFSNSEEAAVQLTDVVP--- 174
+I A + VCH PA+ + G ++GK +TGF++ E + + ++
Sbjct 124 ASEIYANGGVVAAVCHGPAIFDGLTDKKTGRPLIEGKSITGFTDVGETILGVDSILKAKN 183
Query 175 -FLIEDEFKAQGAHY-EKGADWQSFTVREGQLITGQNPASSEAVAEHLLALLK 225
+ED K GA Y W +++ +G+L+TG NPAS+ + A + LK
Sbjct 184 LATVEDVAKKYGAKYLAPVGPWDDYSITDGRLVTGVNPASAHSTAVRSIDALK 236
>O74914 Glutathione-independent glyoxalase hsp3101 [Schizosaccharomyces
pombe (strain 972 / ATCC 24843)]
Length=244
Score = 84.3 bits (207), Expect = 2e-23
Identities = 65/228 (29%), Positives = 110/228 (48%), Gaps = 12/228 (5%)
Query 2 KILMVLTSH-DQLGNTGHKTGFWLEEFTSPYYVFKDAGADITLASPKGGQPPLDPKSNDP 60
K+L+V +S+ G TG E PY VF++AG ++ L S + G+ D S
Sbjct 6 KVLLVASSYYGPFYPDGMNTGVHFAELLIPYQVFREAGYEVQLTS-ETGKCKFDDHSIKK 64
Query 61 DAQTDSTRRF--EQDKQAQQALANTVLLDQVQAEDFDAVFYPGGHGPLWDLTNDKKSIEL 118
A + R +D + AL + D++ ++F +F GGH ++DL + L
Sbjct 65 SALGEVERDAFDNKDNEFWYALKDIKPADKINYKEFCIMFIAGGHAAMFDLPHATNLQTL 124
Query 119 IEQITAMDKPLGLVCHAPAVL---KNVKTAAGDSFVKGKHVTGFSNSEEAAVQLTDVVPF 175
+QI A + L VCH P +L + K+ G S V GK VT F+++ E + ++ +
Sbjct 125 AQQIYASNGVLAAVCHGPVMLPFVDDTKSPEGRSVVYGKKVTAFNSTGELVMGVSSALRE 184
Query 176 L----IEDEFKAQGAHY-EKGADWQSFTVREGQLITGQNPASSEAVAE 218
+ F+ GA + + FT +G+++TG NP S+++ AE
Sbjct 185 RNMQDLNSLFREAGAEFVDPPTPMSDFTQVDGRIVTGVNPMSAKSTAE 232
>Q10092 Probable glutathione-independent glyoxalase hsp3104 [Schizosaccharomyces
pombe (strain 972 / ATCC 24843)]
Length=222
Score = 75.1 bits (183), Expect = 3e-20
Identities = 52/183 (28%), Positives = 84/183 (46%), Gaps = 13/183 (7%)
Query 54 DPKSNDPDAQTDSTRRFEQDKQAQ--QALANTVLLDQVQAEDFDAVFYPGGHGPLWDLTN 111
D S A +DS + +DK +A+ N + D+ F GGHG L+D +
Sbjct 43 DDHSISDSALSDSDKNAFKDKNNDFWKAIKNAKNASDINFSDYSIFFAAGGHGTLFDFPS 102
Query 112 DKKSIELIEQITAMDKPLGLVCHAPAVLKNVKTAAGDSFVKGKHVTGFSNSEEAAVQLTD 171
+ +I +M + VCH P +L +K + G S VKGK VT F+ E A Q +
Sbjct 103 ATNLHKGAAKIYSMGGVIAAVCHGPVILPCIKDSTGFSIVKGKTVTAFN---EIAEQQMN 159
Query 172 VVPFLIEDEFKAQGAHYEKGAD--------WQSFTVREGQLITGQNPASSEAVAEHLLAL 223
++P + FK +++ + F +G+L+TG NPAS+ + A+ L
Sbjct 160 LMPTFEKYHFKTLNKLFQEAGSNFVDPQEPFDDFVKTDGKLVTGANPASAASTAKAALNS 219
Query 224 LKA 226
L +
Sbjct 220 LNS 222
>O43084 Probable glutathione-independent glyoxalase hsp3103 [Schizosaccharomyces
pombe (strain 972 / ATCC 24843)]
Length=261
Score = 73.6 bits (179), Expect = 2e-19
Identities = 62/239 (26%), Positives = 101/239 (42%), Gaps = 31/239 (13%)
Query 17 GHKTGFWLEEFTSPYYVFKDAGADITLASPKG---------GQPPLDPKS--------ND 59
G TG + E PY VF+DA ++ + + G P +D S +D
Sbjct 23 GENTGAFFLELLHPYLVFRDACFNVDIVTESGKIQFDDHSVAGPAIDKGSKGEEFLSYDD 82
Query 60 PDAQTDSTRRFEQ------DKQAQQALANTVLLDQVQAEDFDAVFYPGGHGPLWDLTNDK 113
A + E+ D + + N+ D+V + +D F GGH L+D
Sbjct 83 HIASGPELSKAEKYVLENKDDMFWRIVQNSKTADEVNPDKYDIFFVAGGHATLFDFPKAT 142
Query 114 KSIELIEQITAMDKPLGLVCHAPAVLKNVKTAAGD---SFVKGKHVTGFSNSEEAAVQLT 170
+L I + VCH P +L +K D S V GK VT F E +L
Sbjct 143 NLQKLGTSIYENGGVVAAVCHGPTLLPFMKRQTSDGSVSIVCGKDVTAFDRVAEDKSKLM 202
Query 171 DVVP----FLIEDEFKAQGAHYEKGAD-WQSFTVREGQLITGQNPASSEAVAEHLLALL 224
+ + +++D GA++ K + + F + +G+L+TG NPAS+ + A+ L +L
Sbjct 203 EALKKYNLEVLDDMLNDAGANFIKSPNPFGDFVIADGRLVTGSNPASATSTAKTALRVL 261
>Q08914 Probable glutathione-independent glyoxalase HSP33 [Saccharomyces
cerevisiae (strain ATCC 204508 / S288c)]
Length=237
Score = 71.2 bits (173), Expect = 1e-18
Identities = 62/225 (28%), Positives = 98/225 (44%), Gaps = 18/225 (8%)
Query 2 KILMVLTS-HDQLGNTGHKTGFWLEEFTSPYYVFKDAGADITLASPKGG---QPPLDPKS 57
+ L+ LTS H G KTG ++ E + F+ G ++ S GG PKS
Sbjct 5 RALISLTSYHGPFYKDGAKTGVFVVEILRSFDTFEKHGFEVDFVSETGGFGWDEHYLPKS 64
Query 58 NDPDAQTDSTRRFEQDKQA-QQALANTVLLDQVQAEDFDAVFYPGGHGPLWDLTNDKKSI 116
+ FE A +ALA ++V A D+ F GHG L+D K
Sbjct 65 F---IGGEDKMNFETKNSAFNKALARIKTANEVNASDYKVFFASAGHGALFDYPKAKNLQ 121
Query 117 ELIEQITAMDKPLGLVCHAPAV---LKNVKTAAGDSFVKGKHVTGFSNSEEAAVQLTDVV 173
++ +I A + +CH P + L ++KT ++GK +TGF E A+ + D++
Sbjct 122 DIASKIYANGGVIAAICHGPLLFDGLIDIKTTR--PLIEGKAITGFPLEGEIALGVDDIL 179
Query 174 P----FLIEDEFKAQGAHYEKGA-DWQSFTVREGQLITGQNPASS 213
+E GA Y W +++ +G+L+TG N SS
Sbjct 180 RSRKLTTVERVANKNGAKYLAPIHPWDDYSITDGKLVTGVNANSS 224
>Q08992 Probable glutathione-independent glyoxalase HSP32 [Saccharomyces
cerevisiae (strain ATCC 204508 / S288c)]
Length=237
Score = 70.9 bits (172), Expect = 1e-18
Identities = 62/225 (28%), Positives = 98/225 (44%), Gaps = 18/225 (8%)
Query 2 KILMVLTS-HDQLGNTGHKTGFWLEEFTSPYYVFKDAGADITLASPKGG---QPPLDPKS 57
+ L+ LTS H G KTG ++ E + F+ G ++ S GG PKS
Sbjct 5 RALISLTSYHGPFYKDGAKTGVFVVEILRSFDTFEKHGFEVDFVSETGGFGWDEHYLPKS 64
Query 58 NDPDAQTDSTRRFEQDKQA-QQALANTVLLDQVQAEDFDAVFYPGGHGPLWDLTNDKKSI 116
+ FE A +ALA ++V A D+ F GHG L+D K
Sbjct 65 F---IGGEDKMNFETKNSAFNKALARIKTANEVNASDYKIFFASAGHGALFDYPKAKNLQ 121
Query 117 ELIEQITAMDKPLGLVCHAPAV---LKNVKTAAGDSFVKGKHVTGFSNSEEAAVQLTDVV 173
++ +I A + +CH P + L ++KT ++GK +TGF E A+ + D++
Sbjct 122 DIASKIYANGGVIAAICHGPLLFDGLIDIKTTR--PLIEGKAITGFPLEGEIALGVDDIL 179
Query 174 P----FLIEDEFKAQGAHYEKGA-DWQSFTVREGQLITGQNPASS 213
+E GA Y W +++ +G+L+TG N SS
Sbjct 180 RSRKLTTVERVANKNGAKYLAPIHPWDDYSITDGKLVTGVNANSS 224
>Q04902 Probable glutathione-independent glyoxalase SNO4 [Saccharomyces
cerevisiae (strain ATCC 204508 / S288c)]
Length=237
Score = 67.8 bits (164), Expect = 2e-17
Identities = 61/225 (27%), Positives = 97/225 (43%), Gaps = 18/225 (8%)
Query 2 KILMVLTS-HDQLGNTGHKTGFWLEEFTSPYYVFKDAGADITLASPKGG---QPPLDPKS 57
+ L+ LTS H G KTG ++ E + F+ G ++ S GG PKS
Sbjct 5 RALISLTSYHGPFYKDGAKTGVFVVEILRSFDTFEKHGFEVDFVSETGGFGWDEHYLPKS 64
Query 58 NDPDAQTDSTRRFEQDKQA-QQALANTVLLDQVQAEDFDAVFYPGGHGPLWDLTNDKKSI 116
+ FE A +ALA ++V A D+ F GHG L+D K
Sbjct 65 F---IGGEDKMNFETKNSAFNKALARIKTANEVNASDYKIFFASAGHGALFDYPKAKNLQ 121
Query 117 ELIEQITAMDKPLGLVCHAPAV---LKNVKTAAGDSFVKGKHVTGFSNSEEAAVQLTDVV 173
++ +I A + +CH P + L ++KT ++GK +TGF E A+ + D++
Sbjct 122 DIASKIYANGGVIAAICHGPLLFDGLIDIKTTR--PLIEGKAITGFPLEGEIALGVDDIL 179
Query 174 P----FLIEDEFKAQGAHYEKGA-DWQSFTVREGQLITGQNPASS 213
+E A Y W +++ +G+L+TG N SS
Sbjct 180 RSRKLTTVERVANKNRAKYLAPIHPWDDYSITDGKLVTGVNANSS 224
>P80876 General stress protein 18 [Bacillus subtilis (strain 168)]
Length=172
Score = 53.5 bits (127), Expect = 1e-12
Identities = 47/204 (23%), Positives = 77/204 (38%), Gaps = 53/204 (26%)
Query 26 EFTSPYYVFKDAGADITLASPKGGQPPLDPKSNDPDAQTDSTRRFEQDKQAQQALANTVL 85
E+T P FK+AG ++T+ + G+ + KQ +
Sbjct 17 EYTEPAKAFKEAGHELTVIEKEKGKTV-------------------KGKQGTAEVTVDAS 57
Query 86 LDQVQAEDFDAVFYPGGHGPLWDLTNDKKSIELIEQITAMDKPLGLVCHAPAVLKNVKTA 145
+D V + DFDA+ PGG P L D + ++ + KP+ +CH P +L N K
Sbjct 58 IDDVNSSDFDALLIPGGFSP-DQLRADDRFVQFTKAFMTDKKPVFAICHGPQLLINAKA- 115
Query 146 AGDSFVKGKHVTGFS----NSEEAAVQLTDVVPFLIEDEFKAQGAHYEKGADWQSFTVRE 201
+ G+ TG++ + E A + D + V +
Sbjct 116 -----LDGRKATGYTSIRVDMENAGADVVD-----------------------KEVVVCQ 147
Query 202 GQLITGQNPASSEAVAEHLLALLK 225
QL+T + P A LALL+
Sbjct 148 DQLVTSRTPDDIPAFNRESLALLE 171
>O06006 Putative cysteine protease YraA [Bacillus subtilis (strain
168)]
Length=169
Score = 48.5 bits (114), Expect = 6e-11
Identities = 49/201 (24%), Positives = 80/201 (40%), Gaps = 49/201 (24%)
Query 26 EFTSPYYVFKDAGADITLASPKGGQPPLDPKSNDPDAQTDSTRRFEQDKQAQQALANTVL 85
E+TSP +++AG + D +A + T + + + +A+++
Sbjct 17 EYTSPVKAYEEAGYSVVAI--------------DLEAGKEVTGKHGEKVKIDKAISD--- 59
Query 86 LDQVQAEDFDAVFYPGGHGPLWDLTN-DKKSIELIEQITAMDKPLGLVCHAPAVLKNVKT 144
V A DFDA+ PGG P DL D + E + KP+ +CH P VL +
Sbjct 60 ---VDASDFDALLIPGGFSP--DLLRADDRPGEFAKAFVENKKPVFAICHGPQVLIDT-- 112
Query 145 AAGDSFVKGKHVTGFSNSEEAAVQLTDVVPFLIEDEFKAQGAHYEKGADWQSFTVREGQL 204
+KGK +TG+ + I + GA+Y+ + V +
Sbjct 113 ----DLLKGKDITGYRS---------------IRKDLINAGANYK-----DAEVVVSHNI 148
Query 205 ITGQNPASSEAVAEHLLALLK 225
+T + P EA L LLK
Sbjct 149 VTSRTPDDLEAFNRESLNLLK 169
Lambda K H a alpha
0.317 0.133 0.394 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 353808
Database: 76c61b692aee54fa6d4e0157ef6a879e.SwissProt.fasta
Posted date: May 9, 2024 5:34 PM
Number of letters in database: 2,252
Number of sequences in database: 10
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
