BLASTP 2.12.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.


Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.



Database: f13c25a23d9243c122c7a9528808d490.SwissProt.fasta
           3 sequences; 1,297 total letters



Query= ACIAD2818

Length=424
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

P42308 Citrate transporter [Bacillus subtilis (strain 168)]           448     2e-160
P55069 Mg(2+)/citrate complex secondary transporter [Bacillus sub...  409     4e-145
O05407 Uncharacterized transporter YraO [Bacillus subtilis (strai...  369     6e-129


>P42308 Citrate transporter [Bacillus subtilis (strain 168)]
Length=426

 Score = 448 bits (1153),  Expect = 2e-160
 Identities = 218/427 (51%), Positives = 304/427 (71%), Gaps = 7/427 (2%)

Query  1    MLVFLSYSMIAIFMYAVMSKRLSALTALVLIPVIFAIFTGHSAELSDMMVDGVKKLAPTG  60
            ML  L + M+ +FMY +MS RLSAL AL+++P++FA+ +G   +L +MM+ GV  LAPTG
Sbjct  1    MLAILGFVMMIVFMYLIMSNRLSALIALIVVPIVFALISGFGKDLGEMMIQGVTDLAPTG  60

Query  61   IMITFAILYFCMMTDAGLFNPLIKLIIKIVKGDPLKIVVGTGILGICVGLDGDGATTYII  120
            IM+ FAILYF +M D+GLF+PLI  I+  VKGDPLKI VGT +L + + LDGDG TTY+I
Sbjct  61   IMLLFAILYFGIMIDSGLFDPLIAKILSFVKGDPLKIAVGTAVLTMTISLDGDGTTTYMI  120

Query  121  VATALLPLYTKIGIRPQVMATVLLLSIGVMNILPWAGPFSRAASALKIDPTELFLQMIPV  180
               A+LPLY ++G+   V+A + +L  GVMNI+PW GP +R  ++LK+D +E+F  +IP 
Sbjct  121  TIAAMLPLYKRLGMNRLVLAGIAMLGSGVMNIIPWGGPTARVLASLKLDTSEVFTPLIPA  180

Query  181  MAAGLLWVLFVAFYLGLKERKRLGIHQIEHNEELIHEEPNIPTWK----SWFNLALTALL  236
            M AG+LWV+ VA+ LG KERKRLG+  I+H       +P     K     WFNL LT  L
Sbjct  181  MIAGILWVIAVAYILGKKERKRLGVISIDHAPS---SDPEAAPLKRPALQWFNLLLTVAL  237

Query  237  IFVMIKSYISLAPLFIIAFCIGLVVNFPQLSEQKKLIGKYADSVLAVSTLIFAAGIFVGI  296
            +  +I S + L  LF+ AF + L+VN+P + EQ+K I  +A + L V +++FAAGIF GI
Sbjct  238  MAALITSLLPLPVLFMTAFAVALMVNYPNVKEQQKRISAHAGNALNVVSMVFAAGIFTGI  297

Query  297  MSGTGMIDAIAQHLISIIPESSGNFLASITAFISMPFTYILTNDAFYFGILPILAETASH  356
            +SGT M+DA+A  L+S+IP++ G  L  ITA +SMPFT+ ++NDAFYFG+LPI+AE AS 
Sbjct  298  LSGTKMVDAMAHSLVSLIPDAMGPHLPLITAIVSMPFTFFMSNDAFYFGVLPIIAEAASA  357

Query  357  YGVGANEMAIASLIGQPIHLLSPMVASTYLLCSLTNVDYAENQKNSILWCTGTCMIMYVA  416
            YG+ A E+  ASL+GQP+HLLSP+V STYLL  +  V + ++QK +I W  GT ++M +A
Sbjct  358  YGIDAAEIGRASLLGQPVHLLSPLVPSTYLLVGMAGVSFGDHQKFTIKWAVGTTIVMTIA  417

Query  417  AILIGLI  423
            A+LIG+I
Sbjct  418  ALLIGII  424


>P55069 Mg(2+)/citrate complex secondary transporter [Bacillus 
subtilis (strain 168)]
Length=433

 Score = 409 bits (1052),  Expect = 4e-145
 Identities = 210/431 (49%), Positives = 297/431 (69%), Gaps = 10/431 (2%)

Query  1    MLVFLSYSMIAIFMYAVMSKRLSALTALVLIPVIFAIFTGHS-AELSDMMVDGVKKLAPT  59
            ML  L + M+ +FM  +M+KRLS LTALVL P++FA+  G    E+ DMM+ G++++APT
Sbjct  1    MLAILGFLMMLVFMALIMTKRLSVLTALVLTPIVFALIAGFGFTEVGDMMISGIQQVAPT  60

Query  60   GIMITFAILYFCMMTDAGLFNPLIKLIIKIVKGDPLKIVVGTGILGICVGLDGDGATTYI  119
             +MI FAILYF +M D GLF+P++  I+ +VKGDPLKIVVGT +L + V LDGDG+TTY+
Sbjct  61   AVMIMFAILYFGIMIDTGLFDPMVGKILSMVKGDPLKIVVGTAVLTMLVALDGDGSTTYM  120

Query  120  IVATALLPLYTKIGIRPQVMATVLLLSIGVMNILPWAGPFSRAASALKIDPTELFLQMIP  179
            I  +A+LPLY  +GIRP ++A +  + +G+MN +PW G   RAASAL +DP EL   MIP
Sbjct  121  ITTSAMLPLYLLLGIRPIILAGIAGVGMGIMNTIPWGGATPRAASALGVDPAELTGPMIP  180

Query  180  VMAAGLLWVLFVAFYLGLKERKRLGIHQIEHNEELIHEEPNIPT---WKS----WFNLAL  232
            V+A+G+L ++ VA+ LG  ERKRLG+  IE  +     EP       WK     WFNL L
Sbjct  181  VIASGMLCMVAVAYVLGKAERKRLGV--IELKQPANANEPAAAVEDEWKRPKLWWFNLLL  238

Query  233  TALLIFVMIKSYISLAPLFIIAFCIGLVVNFPQLSEQKKLIGKYADSVLAVSTLIFAAGI  292
            T  LI  ++   +SL  LF+IAFCI L+VN+P L  Q++ I  ++ +VLA+ ++IFAAG+
Sbjct  239  TLSLIGCLVSGKVSLTVLFVIAFCIALIVNYPNLEHQRQRIAAHSSNVLAIGSMIFAAGV  298

Query  293  FVGIMSGTGMIDAIAQHLISIIPESSGNFLASITAFISMPFTYILTNDAFYFGILPILAE  352
            F GI++GT M+D +A  L+S+IPE  G  + +I A  S  FT+++ NDA+++G+LPIL+E
Sbjct  299  FTGILTGTKMVDEMAISLVSMIPEQMGGLIPAIVALTSGIFTFLMPNDAYFYGVLPILSE  358

Query  353  TASHYGVGANEMAIASLIGQPIHLLSPMVASTYLLCSLTNVDYAENQKNSILWCTGTCMI  412
            TA  YGV   E+A AS+IGQPIH+LSP+V ST+LL  L  V   ++QK ++ W     ++
Sbjct  359  TAVAYGVDKVEIARASIIGQPIHMLSPLVPSTHLLVGLVGVSIDDHQKFALKWAVLAVIV  418

Query  413  MYVAAILIGLI  423
            M   A+LIG I
Sbjct  419  MTAIALLIGAI  429


 Score = 19.2 bits (38),  Expect = 0.71
 Identities = 34/152 (22%), Positives = 57/152 (38%), Gaps = 33/152 (22%)

Query  16   AVMSKRLSALTALVLIPVIFA-IFTGH------SAELSDMMVDGVKKLAPTGIMITFAIL  68
            A  S  + A+ +++    +F  I TG       +  L  M+ + +  L P  + +T  I 
Sbjct  280  AAHSSNVLAIGSMIFAAGVFTGILTGTKMVDEMAISLVSMIPEQMGGLIPAIVALTSGIF  339

Query  69   YFCMMTDAGLFNPL--------------IKLIIKIVKGDPLK----IVVGTGILGICVGL  110
             F M  DA  +  L              +++    + G P+     +V  T +L   VG+
Sbjct  340  TFLMPNDAYFYGVLPILSETAVAYGVDKVEIARASIIGQPIHMLSPLVPSTHLLVGLVGV  399

Query  111  DGDG--------ATTYIIVATALLPLYTKIGI  134
              D         A   +IV TA+  L   I I
Sbjct  400  SIDDHQKFALKWAVLAVIVMTAIALLIGAISI  431


>O05407 Uncharacterized transporter YraO [Bacillus subtilis (strain 
168)]
Length=438

 Score = 369 bits (946),  Expect = 6e-129
 Identities = 181/435 (42%), Positives = 289/435 (66%), Gaps = 12/435 (3%)

Query  1    MLVFLSYSMIAIFMYAVMSKRLSALTALVLIPVIFAIFTGHSAELSDMMVDGVKKLAPTG  60
            ML  L +SM+ +F   +M+K++S + AL + P++FA+  G    + DM+++G++ +A + 
Sbjct  1    MLTILGFSMVTVFTILIMTKKVSPIVALTITPIVFALIGGFGKGIGDMILEGIQTVASSA  60

Query  61   IMITFAILYFCMMTDAGLFNPLIKLIIKIVKGDPLKIVVGTGILGICVGLDGDGATTYII  120
             ++ FAIL+F ++ DAGLF+PLI+ I+ IVKGDP+KI +G+ +L + + LDGDG TTY+I
Sbjct  61   ALLLFAILFFGILIDAGLFDPLIEKILSIVKGDPVKIAIGSAVLAMLIALDGDGTTTYMI  120

Query  121  VATALLPLYTKIGIRPQVMATVLLLSIGVMN-ILPWAGPFSRAASALKIDPTELFLQMIP  179
              +A+LPLY +IG+ P VMAT+ +LS+ +++ + PW GP +RA S L +DP++ F+ ++P
Sbjct  121  TVSAMLPLYKRIGMNPMVMATLAMLSLSIVSGMTPWGGPATRAISVLGLDPSDFFVPLLP  180

Query  180  VMAAGLLWVLFVAFYLGLKERKRLGIHQIE--HNEELIHEEPNIPTWKS---------WF  228
             M  G+  V+F+AF +G KER R+GI Q+E  H  +   +     T +S         + 
Sbjct  181  TMLGGIACVIFLAFLMGRKERNRIGIVQLEPRHITKDSSQSYMAATLESEQLKRPRLIYL  240

Query  229  NLALTALLIFVMIKSYISLAPLFIIAFCIGLVVNFPQLSEQKKLIGKYADSVLAVSTLIF  288
            NL L   ++  ++      + LF+I F + L +N+P +  QK+ I +++ + + V  L+F
Sbjct  241  NLFLVISIMVFIVLGTKHPSVLFLIGFVLALTINYPNVKMQKERIAEHSGNAITVVLLVF  300

Query  289  AAGIFVGIMSGTGMIDAIAQHLISIIPESSGNFLASITAFISMPFTYILTNDAFYFGILP  348
            +AG+F GI+SGT M+DAIA  LISIIP S G F   I A  S+PFT++L+NDA+YFG++P
Sbjct  301  SAGVFAGILSGTKMVDAIAGSLISIIPSSMGGFFPVIVALTSIPFTFVLSNDAYYFGMVP  360

Query  349  ILAETASHYGVGANEMAIASLIGQPIHLLSPMVASTYLLCSLTNVDYAENQKNSILWCTG  408
            I AE AS YG+   E+A AS++GQP+HL+SP+VAST LL S+  +D    Q+ ++ W   
Sbjct  361  IFAEAASAYGIEPVEIARASIMGQPVHLMSPLVASTVLLVSMLKMDLGSFQRFAVKWAVI  420

Query  409  TCMIMYVAAILIGLI  423
            T +++ + AI+ G I
Sbjct  421  TSLVITLLAIITGAI  435



Lambda      K        H        a         alpha
   0.330    0.143    0.427    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 454300


  Database: f13c25a23d9243c122c7a9528808d490.SwissProt.fasta
    Posted date:  May 9, 2024  4:37 AM
  Number of letters in database: 1,297
  Number of sequences in database:  3



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40