BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 3e9a53b82b8467eb2245b7db47094666.SwissProt.fasta
19 sequences; 8,780 total letters
Query= ACIAD3026
Length=307
Score E
Sequences producing significant alignments: (Bits) Value
O28726 Uncharacterized MscS family protein AF_1546 [Archaeoglobus... 131 2e-39
Q55717 Uncharacterized MscS family protein slr0639 [Synechocystis... 117 3e-34
P0C0S3 Small-conductance mechanosensitive channel [Shigella flexn... 98.2 6e-27
P0C0S2 Small-conductance mechanosensitive channel [Escherichia co... 98.2 6e-27
P0C0S1 Small-conductance mechanosensitive channel [Escherichia co... 98.2 6e-27
O52401 Small-conductance mechanosensitive channel [Edwardsiella i... 97.8 8e-27
Q89AB5 Uncharacterized MscS family protein bbp_402 [Buchnera aphi... 87.8 3e-23
Q57362 Uncharacterized MscS family protein HI_0195.1 [Haemophilus... 87.4 4e-22
P77338 Mechanosensitive channel MscK [Escherichia coli (strain K12)] 84.3 4e-21
Q8K9B1 Uncharacterized MscS family protein BUsg_437 [Buchnera aph... 81.3 7e-21
P57527 Uncharacterized MscS family protein BU452 [Buchnera aphidi... 80.9 1e-20
O66994 Uncharacterized MscS family protein aq_812 [Aquifex aeolic... 68.9 3e-16
Q8R6L9 Small-conductance mechanosensitive channel [Caldanaerobact... 68.2 3e-16
P39285 Miniconductance mechanosensitive channel MscM [Escherichia... 65.1 9e-15
O34897 Uncharacterized MscS family protein YkuT [Bacillus subtili... 62.4 2e-14
Q8VZL4 Mechanosensitive ion channel protein 1, mitochondrial [Ara... 57.4 2e-12
O35043 Uncharacterized MscS family protein YfkC [Bacillus subtili... 52.0 8e-11
P75783 Moderate conductance mechanosensitive channel YbiO [Escher... 48.5 2e-09
P0AAT4 Miniconductance mechanosensitive channel YbdG [Escherichia... 25.4 0.038
>O28726 Uncharacterized MscS family protein AF_1546 [Archaeoglobus
fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
100126 / VC-16)]
Length=283
Score = 131 bits (329), Expect = 2e-39
Identities = 72/247 (29%), Positives = 132/247 (53%), Gaps = 5/247 (2%)
Query 28 ELLVALVLCLIGFIFARLVSNTFIRTIGQRFNAHQRMVWRRGIFYFIFLIFVMASLKEAG 87
+++V +V+ + I A+L++ R + + Q + + I++ I ++ +A L G
Sbjct 20 DIIVVIVVMALATIIAKLITTNLRRALIDKMKRDQLELMLKVIYFGIIIVAFIAVLPALG 79
Query 88 FKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVGDTIQITLIRGHTIEGQV 147
LS L A GI + LGFASQ+ +NL+SG+FLI E ++GD + I + G V
Sbjct 80 LDLSGLLVAGGITGIVLGFASQSVVANLVSGIFLISEKPIKIGDQVNIDGVAGF-----V 134
Query 148 ISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMSKYPIRRIPITLAINFQEDIMKVRKVL 207
+++LS + T D +YVR+PNE++ + + N + RR + I + +D K +++
Sbjct 135 EDVNILSTIIRTYDGLYVRIPNEKVFTSNITNYVAHIARRFEYVVGIRYSDDAEKAIEII 194
Query 208 LDVASKYPMALDDPKPEVTVTAFRESSIELLFTVWCRQEFFLKSRDELQERIRNGFLENQ 267
+ ++P AL +P+P V V +SS+ ++ +W + + EL +I+ +N
Sbjct 195 KRIIEEHPFALKNPEPVVFVDNLGDSSVNIVVRIWAPSTEWYNVKMELLWKIKTELEKNG 254
Query 268 IEIPVPK 274
IEIP P+
Sbjct 255 IEIPFPQ 261
>Q55717 Uncharacterized MscS family protein slr0639 [Synechocystis
sp. (strain PCC 6803 / Kazusa)]
Length=296
Score = 117 bits (294), Expect = 3e-34
Identities = 77/273 (28%), Positives = 134/273 (49%), Gaps = 11/273 (4%)
Query 19 TNINLDRLSELLVALVLCLIGFIFARLVSNTFIRTIGQRFNAHQRMVWRRG--IFYFIFL 76
T++ + ++L AL + LIGF ++ + IR + ++ N + G +Y + +
Sbjct 27 TDLLITASLKILFALAILLIGFWLSKRLQKLIIRAL-RKSNLEPTFISFAGNISYYLLLV 85
Query 77 IFVMASLKEAGFKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVGDTIQIT 136
+F + L + G + S + G T+A+G A Q S +N+ G+ L+ F VG+ I++
Sbjct 86 VFFVLCLAQLGIQTSSLVALLGASTLAIGLALQGSLANVAGGILLVLFNYFRVGERIEVA 145
Query 137 LIRGHTIEGQVISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMSKYPIRRIPITLAINF 196
IEG V SI++LS + T DN V +PN+Q+I ++N P RRI + + + +
Sbjct 146 -----GIEGIVESIEILSTTICTYDNRLVTIPNKQIIENNIINHVGKPERRIDLVIGVGY 200
Query 197 QEDIMKVRKVLLDVASKYPMALDDPKPEVTVTAFRESSIELLFTVWCRQEFFLKSRDELQ 256
+EDI VR L V + +P P + + +SS+ W + E + + + +L
Sbjct 201 EEDIDHVRSSLQWVIDQNSEVCTEPAPTIALGELGDSSVNFYVRPWVKSEDYFRLKLQLT 260
Query 257 ERIRNGFLENQIEIPVPKMGFVDRPAVSPLTNE 289
E I+ E I IP P+ D + P T E
Sbjct 261 EAIKRKLDEENISIPFPQR---DVHLIQPETKE 290
>P0C0S3 Small-conductance mechanosensitive channel [Shigella flexneri]
Length=286
Score = 98.2 bits (243), Expect = 6e-27
Identities = 63/248 (25%), Positives = 117/248 (47%), Gaps = 6/248 (2%)
Query 29 LLVALVLCLIGFIFARLVSNTFIRT-IGQRFNAHQRMVWRRGIFYFIFLIFVMASLKEAG 87
++ AL + ++G I AR++SN R I ++ +A + Y I ++A+L G
Sbjct 31 IVAALAIIIVGLIIARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVG 90
Query 88 FKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVGDTIQITLIRGHTIEGQV 147
+ + + G +A+G A Q S SNL +G+ L+ F G+ + + + G V
Sbjct 91 VQTASVIAVLGAAGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLG-----GVAGTV 145
Query 148 ISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMSKYPIRRIPITLAINFQEDIMKVRKVL 207
+S+ + S + T D + +PN ++I ++N S+ P+RR + + + DI +V+++L
Sbjct 146 LSVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQIL 205
Query 208 LDVASKYPMALDDPKPEVTVTAFRESSIELLFTVWCRQEFFLKSRDELQERIRNGFLENQ 267
++ L D + V + SSI + VW ++ ERI+ F
Sbjct 206 TNIIQSEDRILKDREMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAG 265
Query 268 IEIPVPKM 275
I P P+M
Sbjct 266 ISFPYPQM 273
>P0C0S2 Small-conductance mechanosensitive channel [Escherichia
coli O157:H7]
Length=286
Score = 98.2 bits (243), Expect = 6e-27
Identities = 63/248 (25%), Positives = 117/248 (47%), Gaps = 6/248 (2%)
Query 29 LLVALVLCLIGFIFARLVSNTFIRT-IGQRFNAHQRMVWRRGIFYFIFLIFVMASLKEAG 87
++ AL + ++G I AR++SN R I ++ +A + Y I ++A+L G
Sbjct 31 IVAALAIIIVGLIIARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVG 90
Query 88 FKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVGDTIQITLIRGHTIEGQV 147
+ + + G +A+G A Q S SNL +G+ L+ F G+ + + + G V
Sbjct 91 VQTASVIAVLGAAGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLG-----GVAGTV 145
Query 148 ISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMSKYPIRRIPITLAINFQEDIMKVRKVL 207
+S+ + S + T D + +PN ++I ++N S+ P+RR + + + DI +V+++L
Sbjct 146 LSVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQIL 205
Query 208 LDVASKYPMALDDPKPEVTVTAFRESSIELLFTVWCRQEFFLKSRDELQERIRNGFLENQ 267
++ L D + V + SSI + VW ++ ERI+ F
Sbjct 206 TNIIQSEDRILKDREMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAG 265
Query 268 IEIPVPKM 275
I P P+M
Sbjct 266 ISFPYPQM 273
>P0C0S1 Small-conductance mechanosensitive channel [Escherichia
coli (strain K12)]
Length=286
Score = 98.2 bits (243), Expect = 6e-27
Identities = 63/248 (25%), Positives = 117/248 (47%), Gaps = 6/248 (2%)
Query 29 LLVALVLCLIGFIFARLVSNTFIRT-IGQRFNAHQRMVWRRGIFYFIFLIFVMASLKEAG 87
++ AL + ++G I AR++SN R I ++ +A + Y I ++A+L G
Sbjct 31 IVAALAIIIVGLIIARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVG 90
Query 88 FKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVGDTIQITLIRGHTIEGQV 147
+ + + G +A+G A Q S SNL +G+ L+ F G+ + + + G V
Sbjct 91 VQTASVIAVLGAAGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLG-----GVAGTV 145
Query 148 ISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMSKYPIRRIPITLAINFQEDIMKVRKVL 207
+S+ + S + T D + +PN ++I ++N S+ P+RR + + + DI +V+++L
Sbjct 146 LSVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQIL 205
Query 208 LDVASKYPMALDDPKPEVTVTAFRESSIELLFTVWCRQEFFLKSRDELQERIRNGFLENQ 267
++ L D + V + SSI + VW ++ ERI+ F
Sbjct 206 TNIIQSEDRILKDREMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAG 265
Query 268 IEIPVPKM 275
I P P+M
Sbjct 266 ISFPYPQM 273
>O52401 Small-conductance mechanosensitive channel [Edwardsiella
ictaluri (strain 93-146)]
Length=286
Score = 97.8 bits (242), Expect = 8e-27
Identities = 67/248 (27%), Positives = 116/248 (47%), Gaps = 6/248 (2%)
Query 29 LLVALVLCLIGFIFARLVSNTFIRTIGQRFNAHQRMVWRRGIFYFIFLIF-VMASLKEAG 87
L+ A+V+ +IG + AR +S T IR + R + + + + L F ++A+L G
Sbjct 31 LVAAVVILIIGSLIARGISTTLIRLLKARGLDVTVVHFLAAMVRYAILAFTIIAALGRLG 90
Query 88 FKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVGDTIQITLIRGHTIEGQV 147
+ + + G +A+G A Q S SN +G+ L+ F G+ + + + G V
Sbjct 91 VQTTSVIAVLGAAGLAVGLALQGSLSNFAAGVLLVLFRPFRAGEVVDLG-----GVTGTV 145
Query 148 ISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMSKYPIRRIPITLAINFQEDIMKVRKVL 207
+ + S L T DN + +PN ++I ++N S+ P RRI I + + + DI V++VL
Sbjct 146 REVQIFSTTLATADNKVIVVPNGKIIAGNIINFSREPKRRIDIIVGVAYDADIDVVKRVL 205
Query 208 LDVASKYPMALDDPKPEVTVTAFRESSIELLFTVWCRQEFFLKSRDELQERIRNGFLENQ 267
DV + L D + + SS+ + VW + +L E + N
Sbjct 206 GDVVAADTRILHDDGVTIRLNEMAASSLNFVVRVWGNNADYWAIYFDLMENFKRALDANN 265
Query 268 IEIPVPKM 275
I IP P+M
Sbjct 266 IGIPFPQM 273
>Q89AB5 Uncharacterized MscS family protein bbp_402 [Buchnera
aphidicola subsp. Baizongia pistaciae (strain Bp)]
Length=281
Score = 87.8 bits (216), Expect = 3e-23
Identities = 66/277 (24%), Positives = 122/277 (44%), Gaps = 8/277 (3%)
Query 1 MADSNIPKDVAVSLKGIFTN--INLDRLSELLVALVLCLIGFIFARLVSNTFIRT-IGQR 57
M N+ D+ + + N + L + L+ ++++ +IGF A+++SN + I Q+
Sbjct 1 MTQLNVVNDINHAGNWLIRNQELLLSYIINLISSIIILIIGFFAAKIISNLINKVLITQK 60
Query 58 FNAHQRMVWRRGIFYFIFLIFVMASLKEAGFKLSVFLGAAGILTVALGFASQTSASNLIS 117
+ + Y I ++ASL G + + + G +A+G A Q S SN +
Sbjct 61 IDTTIANFLAALVRYIIITFALIASLGCIGVQTTSVIAILGAAGMAIGLALQGSLSNFAA 120
Query 118 GLFLIGEGSFEVGDTIQITLIRGHTIEGQVISIDLLSVKLLTLDNVYVRLPNEQLIRAPV 177
G+ L+ F G+ + + I G V++I + TLD V +PN ++I +
Sbjct 121 GVLLVILRPFRTGEYVNL-----EKISGTVLNIHVFYTTFRTLDGKIVVIPNGKIISGNI 175
Query 178 MNMSKYPIRRIPITLAINFQEDIMKVRKVLLDVASKYPMALDDPKPEVTVTAFRESSIEL 237
+N S+ RR + +++ DI KV K+L +V L D V ++ SS+
Sbjct 176 INYSREKARRNEFIIGVSYDSDIDKVIKILKNVVKNEKRVLKDRDIIVGLSELAPSSLNF 235
Query 238 LFTVWCRQEFFLKSRDELQERIRNGFLENQIEIPVPK 274
+ W + +L + + N I IP P+
Sbjct 236 IVRCWSHTDDINIVYWDLMVKFKKALDSNNINIPYPQ 272
>Q57362 Uncharacterized MscS family protein HI_0195.1 [Haemophilus
influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)]
Length=1111
Score = 87.4 bits (215), Expect = 4e-22
Identities = 73/252 (29%), Positives = 116/252 (46%), Gaps = 15/252 (6%)
Query 26 LSELLVALVLCLIGFIFARLVSNTFIRTIGQRFNAHQRMVWR-RGIFYFIFLI----FVM 80
L LLVALV+ I ++ R +S I R N Q + + +IF+ +
Sbjct 843 LFNLLVALVIVGITYVLVRNISGILEVLIFSRVNLSQGTPYTITTLLTYIFIAIGGAWAF 902
Query 81 ASLKEAGFKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVGDTIQITLIRG 140
A+L + KL A L+V LGF Q +N +SG+ L+ E VGD + I
Sbjct 903 ATLGMSWSKLQWLFAA---LSVGLGFGMQEIFANFVSGIILLFERPIRVGDVVTI----- 954
Query 141 HTIEGQVISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMS-KYPIRRIPITLAINFQED 199
+ + G V I + ++ L+ D V +PN+ + V N + + R+ I++ + + D
Sbjct 955 NEVSGTVAKIRIRAITLIDFDRKEVIVPNKSFVTGQVTNWALSNTMTRLVISVGVAYGSD 1014
Query 200 IMKVRKVLLDVASKYPMALDDPKPEVTVTAFRESSIELLFTVWCRQEFFLKS-RDELQER 258
+ VR++LL A + P L DPKP F S+++ V+ Q S D L R
Sbjct 1015 LTLVRQLLLQAADEQPTILRDPKPSAYFLTFGASTLDHELRVYVEQVGDRTSTTDALNRR 1074
Query 259 IRNGFLENQIEI 270
I F E+ I+I
Sbjct 1075 INELFAEHNIDI 1086
Score = 18.9 bits (37), Expect = 4.7
Identities = 8/19 (42%), Positives = 15/19 (79%), Gaps = 2/19 (11%)
Query 69 GIFYFIF--LIFVMASLKE 85
GIFY+++ L+ V++ L+E
Sbjct 804 GIFYYVWSDLVTVVSYLRE 822
>P77338 Mechanosensitive channel MscK [Escherichia coli (strain
K12)]
Length=1120
Score = 84.3 bits (207), Expect = 4e-21
Identities = 68/252 (27%), Positives = 109/252 (43%), Gaps = 15/252 (6%)
Query 26 LSELLVALVLCLIGFIFARLVSNTFIRTIGQRFNAHQRMVWRRGIF--YFIFLIFVMASL 83
+ LL A++ ++ + R + + R N Q + Y I + M
Sbjct 843 MGSLLFAIIASMVAWALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVF 902
Query 84 KEAGFKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVGDTIQITLIRGHTI 143
G A L+V LGF Q N +SGL ++ E +GDT+ I +
Sbjct 903 GSLGVSWDKLQWLAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIG-----SF 957
Query 144 EGQVISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMSKYPIR-RIPITLAINFQEDIMK 202
G V I + + + D V +PN+ + ++N S R+ I L + + D+ K
Sbjct 958 SGTVSKIRIRATTITDFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEK 1017
Query 203 VRKVLLDVASKYPMALDDPKPEVTVTAFRESSIELLFTVWCRQEFFLKSR----DELQER 258
VRKVLL A+++P + +P PEV TAF S+++ ++ R+ L+ R DEL
Sbjct 1018 VRKVLLKAATEHPRVMHEPMPEVFFTAFGASTLDHELRLYVRE---LRDRSRTVDELNRT 1074
Query 259 IRNGFLENQIEI 270
I EN I I
Sbjct 1075 IDQLCRENDINI 1086
>Q8K9B1 Uncharacterized MscS family protein BUsg_437 [Buchnera
aphidicola subsp. Schizaphis graminum (strain Sg)]
Length=283
Score = 81.3 bits (199), Expect = 7e-21
Identities = 57/248 (23%), Positives = 112/248 (45%), Gaps = 6/248 (2%)
Query 29 LLVALVLCLIGFIFARLVSNTFIRTIGQRFNAHQRMVWRRGIFYFIFLIFVM-ASLKEAG 87
L A+++ + G A+++SN + + R + + +I + F + ASL G
Sbjct 31 LTSAIIILISGMFIAKIISNGVNQILITRHIDATIAGFLSALMRYIIITFTLIASLGRIG 90
Query 88 FKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVGDTIQITLIRGHTIEGQV 147
+ + + G +A+G A Q S SN +G+ L+ + G+ + + + G V
Sbjct 91 VQTTSVIAILGAAGMAIGLALQGSLSNFAAGVLLVTLRPLKTGEYVNLG-----NVAGTV 145
Query 148 ISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMSKYPIRRIPITLAINFQEDIMKVRKVL 207
++I + L TLD V +PN ++I ++N S+ P RR ++++++ DI V KVL
Sbjct 146 LNIHIFYTTLRTLDGKIVVVPNNKIISGNIINYSREPARRNEFSISVSYNTDIDLVIKVL 205
Query 208 LDVASKYPMALDDPKPEVTVTAFRESSIELLFTVWCRQEFFLKSRDELQERIRNGFLENQ 267
V + D + ++ SS+ + W + +L + + +N
Sbjct 206 KRVIENEDRVMKDRDIVIGLSELAPSSLNFIIRCWSSTDELNAVYWDLMVKFKKELDKNN 265
Query 268 IEIPVPKM 275
I IP P++
Sbjct 266 INIPYPQI 273
>P57527 Uncharacterized MscS family protein BU452 [Buchnera aphidicola
subsp. Acyrthosiphon pisum (strain APS)]
Length=305
Score = 80.9 bits (198), Expect = 1e-20
Identities = 58/252 (23%), Positives = 111/252 (44%), Gaps = 14/252 (6%)
Query 29 LLVALVLCLIGFIFARLVSN-----TFIRTIGQRFNAHQRMVWRRGIFYFIFLIFVMASL 83
L A+++ ++G ++++SN R I + R I F F+ A+L
Sbjct 55 LTSAIIILVVGMFISKIISNGANQVLITRNIDATIAGFLSALMRYIIITFTFI----AAL 110
Query 84 KEAGFKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVGDTIQITLIRGHTI 143
G + + + G +A+G A Q S SN +G+ L+ + + + + ++
Sbjct 111 GRIGVQTTSVIAILGAAGMAIGLALQGSLSNFAAGVLLVTLRPLKTEEYVDLG-----SV 165
Query 144 EGQVISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMSKYPIRRIPITLAINFQEDIMKV 203
G V++I + L TLD V +PN ++I ++N S+ P RR +++++ DI V
Sbjct 166 SGTVLNIHIFYTTLRTLDGKIVVVPNNKIISGNIINYSREPARRNEFIISVSYNSDIDLV 225
Query 204 RKVLLDVASKYPMALDDPKPEVTVTAFRESSIELLFTVWCRQEFFLKSRDELQERIRNGF 263
K+L V K + D V ++ SS+ + W + +L + +
Sbjct 226 IKILRSVIEKEERVIKDKDIIVGLSELAPSSLNFIVRCWSKNHDLNTVYWDLMAKFKKEL 285
Query 264 LENQIEIPVPKM 275
+N I IP P++
Sbjct 286 DKNNINIPFPQL 297
>O66994 Uncharacterized MscS family protein aq_812 [Aquifex aeolicus
(strain VF5)]
Length=368
Score = 68.9 bits (167), Expect = 3e-16
Identities = 52/249 (21%), Positives = 115/249 (46%), Gaps = 11/249 (4%)
Query 17 IFTNINLDRLSELLVALVLCLIGFIFARLVSNTFIRTIGQRFNAHQRMVWRRGIFYFIFL 76
++ + L S L++ + +F + F++ G+ F + + F+ +
Sbjct 99 VYADKFLKTFSLLILGWAIIRFLNLFHNKIVEFFVKVGGKDFAEEVGDFILKILKAFVVV 158
Query 77 IFVMASLKEAGFKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVGDTIQIT 136
I + L+E G + L + G+L +A+ A++ + N++SGL ++ + +VG+T+++
Sbjct 159 IVGASLLQEWGVNIGAILASVGLLGLAVSLAAKDTFENILSGLIILLDKPVKVGETVKV- 217
Query 137 LIRGHTIEGQVISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMSKYPIRRIPITLAINF 196
G V I L S K+ T D V +PN ++ V N ++ RR+ + + +
Sbjct 218 ----KDFMGSVEDIGLRSTKIRTFDKSLVTIPNRDIVNNHVENFTRRNKRRVRFYIGVVY 273
Query 197 ---QEDIMKVRKVLLDVASKYPMALDDPKPEVTVTAFRESSIELLFTVWCR---QEFFLK 250
+E + + K + ++ ++P D K V + +SS+ +L + E +LK
Sbjct 274 STKREQLENILKEIRELLKEHPGVAKDEKFYVYFENYGDSSLNILIQYYANTNDYEEYLK 333
Query 251 SRDELQERI 259
+++ +I
Sbjct 334 IIEDINLKI 342
>Q8R6L9 Small-conductance mechanosensitive channel [Caldanaerobacter
subterraneus subsp. tengcongensis (strain DSM 15242 /
JCM 11007 / NBRC 100824 / MB4)]
Length=282
Score = 68.2 bits (165), Expect = 3e-16
Identities = 61/259 (24%), Positives = 117/259 (45%), Gaps = 18/259 (7%)
Query 26 LSELLVALVLCLIGFIFARLVSNTFIRTIGQ-RFNAHQRMV------WRRGIFYFIFLIF 78
L ++L L++ IG FA + F + + + QR + + + Y I+ +
Sbjct 21 LLDVLKILIIAFIGIKFADFLIYRFYKLYSKSKIQLPQRKIDTLTSLTKNAVRYIIYFLA 80
Query 79 VMASLKEAGFKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVGDTIQITLI 138
+ LK ++ L AGI ++A+GF +Q ++ISG F+I E F VGD + I
Sbjct 81 GASILKLFNIDMTSLLAVAGIGSLAIGFGAQNLVKDMISGFFIIFEDQFSVGDYVTI--- 137
Query 139 RGHTIEGQVISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMSKYPIRRIPITLAINFQE 198
+ I G V I L K+ + +PN ++ V N++K + + + +A E
Sbjct 138 --NGISGTVEEIGLRVTKIRGFSDGLHIIPNGEI--KMVTNLTKDSMMAV-VNIAFPIDE 192
Query 199 DIMKVRKVLLDVASKYPMALDDPKPEVTVTAFRE-SSIELLFTVWCRQEFFLK--SRDEL 255
D+ K+ + L ++ + + DD TV + +L+ V+ + + K ++
Sbjct 193 DVDKIIEGLQEICEEVKKSRDDLIEGPTVLGITDMQDSKLVIMVYAKTQPMQKWAVERDI 252
Query 256 QERIRNGFLENQIEIPVPK 274
+ R++ F + I P P+
Sbjct 253 RYRVKKMFDQKNISFPYPR 271
>P39285 Miniconductance mechanosensitive channel MscM [Escherichia
coli (strain K12)]
Length=1107
Score = 65.1 bits (157), Expect = 9e-15
Identities = 51/204 (25%), Positives = 88/204 (43%), Gaps = 7/204 (3%)
Query 72 YFIFLIFVMASLKEAGFKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVGD 131
Y + LI + G + S L V LGF Q +N ISGL ++ E +GD
Sbjct 879 YLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEIFANFISGLIILFEKPIRIGD 938
Query 132 TIQITLIRGHTIEGQVISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMS-KYPIRRIPI 190
T+ I + G V I+ + + D + +PN+ I +N S + R+ +
Sbjct 939 TVTI-----RDLTGSVTKINTRATTISDWDRKEIIVPNKAFITEQFINWSLSDSVTRVVL 993
Query 191 TLAINFQEDIMKVRKVLLDVASKYPMALDDPKPEVTVTAFRES-SIELLFTVWCRQEFFL 249
T+ + +V ++LL A + + +D+P PEV + ++ I L +
Sbjct 994 TIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQQGIQIFELRIYAAEMGHRM 1053
Query 250 KSRDELQERIRNGFLENQIEIPVP 273
R E+ + I GF + I++P P
Sbjct 1054 PLRHEIHQLILAGFHAHGIDMPFP 1077
Score = 18.9 bits (37), Expect = 4.7
Identities = 21/82 (26%), Positives = 37/82 (45%), Gaps = 6/82 (7%)
Query 128 EVGDTIQITLIRGHTIEGQVISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMSKYPIRR 187
EV + +Q L + +E + S++ + +DN Y +L +RA + NM P
Sbjct 44 EVVEALQSAL---NALEERKGSLERIKQYQQVIDN-YPKL--SATLRAQLNNMRDEPRSV 97
Query 188 IPITLAINFQEDIMKVRKVLLD 209
P ++I++V LLD
Sbjct 98 SPGMSTDALNQEILQVSSQLLD 119
>O34897 Uncharacterized MscS family protein YkuT [Bacillus subtilis
(strain 168)]
Length=267
Score = 62.4 bits (150), Expect = 2e-14
Identities = 63/262 (24%), Positives = 120/262 (46%), Gaps = 28/262 (11%)
Query 16 GIFTNINLDRLSELLVALVLCLIGFIFARLVSNTFIRTIGQRFN---------AHQRMVW 66
G+ TN + LL+ L + ++ + R + I+ + +F AH
Sbjct 11 GLITNAGV-----LLIKLAIMILLYFIVRSLGMKIIKHLFAKFEEQNSLSIGRAHTLRSL 65
Query 67 RRGIFYF--IFLIFVMASLKEAGFKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGE 124
IF + IF+ FVM L + S L AGI+ +A+GF +Q S++++G F++ E
Sbjct 66 TLNIFAYTLIFIFFVMV-LDLFHYDPSALLAGAGIVGLAVGFGAQGLVSDIVTGFFILLE 124
Query 125 GSFEVGDTIQITLIRGHTIEGQVISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMSKYP 184
+VGD I ++ T +G V + L + ++ + D +PN + V N S+
Sbjct 125 KQLDVGDYITVS-----TFDGIVEQVGLRTTQIRSFDGTLHYIPNRNITN--VSNHSRGT 177
Query 185 IRRIPITLAINFQEDIMKVRKVLLDVASKYPMALDDPKPEVTVTAFRE-SSIELLFTVWC 243
++ + + + + + +I ++ +L V + AL K V +E + E++ V
Sbjct 178 MQAL-VDIKVPAERNIDEMIHILQQVCDETAAALPQIKEGPNVIGIQELGTSEIVIRVIA 236
Query 244 RQEFFLKSRDE--LQERIRNGF 263
+ E + R E L++ I+N F
Sbjct 237 KTENMEQWRVERVLRKEIKNAF 258
>Q8VZL4 Mechanosensitive ion channel protein 1, mitochondrial
[Arabidopsis thaliana]
Length=497
Score = 57.4 bits (137), Expect = 2e-12
Identities = 58/242 (24%), Positives = 110/242 (45%), Gaps = 21/242 (9%)
Query 32 ALVLCLIGFIFARLVSNTFIRTIGQR----FNAHQRMVWRRGIFYFIFLIFVMASLKEAG 87
A++L L+ F++ R +N R + + + + + + +F I +MAS + G
Sbjct 246 AVILSLVWFLY-RWKTNVITRMLSAKSFGGLDREKVLTLDKVSSVGLFAIGLMASAEACG 304
Query 88 FKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVGDTIQITLIRGHTIEGQV 147
+ L G+ VA FA++ N++SGL + F +GDT I+ ++EGQV
Sbjct 305 VAVQSILTVGGVGGVATAFAARDILGNVLSGLSMQFSRPFSMGDT-----IKAGSVEGQV 359
Query 148 ISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMSKYPIRRI--PITLAINFQEDIMKVRK 205
I + L + LL + V +PN ++N S+ R I I L I+ + I ++
Sbjct 360 IEMGLTTTSLLNAEKFPVLVPNSLFSSQVIVNKSRAQWRAIASKIPLQIDDLDMIPQISN 419
Query 206 VLLD-VASKYPMALDDPKPEVTVTAFRESSIELLFTVWC------RQEFFLKSRDELQER 258
+ + + S + L P ++ +S EL T+ C ++E + ++ L E
Sbjct 420 EIKEMLRSNTKVFLGKEAPHCYLSRVEKSFAEL--TIGCNLIRMGKEELYNTQQEVLLEA 477
Query 259 IR 260
++
Sbjct 478 VK 479
>O35043 Uncharacterized MscS family protein YfkC [Bacillus subtilis
(strain 168)]
Length=280
Score = 52.0 bits (123), Expect = 8e-11
Identities = 54/202 (27%), Positives = 90/202 (45%), Gaps = 20/202 (10%)
Query 14 LKGIFTNINLDRLSELLVALVLCLIG-FIFARLVSNTFIRT--IGQRFNAHQRMVWRRGI 70
+K T I +++ ++L+ V+ IG FI RLV F RT I ++ + R
Sbjct 3 MKETLTEIFQNKIVDILLVAVILWIGVFIINRLVQLFFKRTDFIEEKKEKTIESLVRSVT 62
Query 71 FYFIFLIFVMASLKEAGFKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVG 130
Y + F+ + L AG+ + +GF +Q+ ++++G+FLI E G
Sbjct 63 QYTATIGFIFYVISLFVHDFGKILAGAGVAGIVIGFGAQSLIKDVLAGVFLIYERQLHKG 122
Query 131 DTIQITLIRGHTIEG---QVISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMNMSKYPI-- 185
D + + + T+E + + I S KLLT+ N VR + Y I
Sbjct 123 DYVTVNNLFNGTVEEIGLRSLQIREWSGKLLTISNGEVR------------QIENYNIDF 170
Query 186 RRIPITLAINFQEDIMKVRKVL 207
RI + I+F+ED +V VL
Sbjct 171 MRITESFLISFKEDPDRVYSVL 192
>P75783 Moderate conductance mechanosensitive channel YbiO [Escherichia
coli (strain K12)]
Length=741
Score = 48.5 bits (114), Expect = 2e-09
Identities = 36/128 (28%), Positives = 59/128 (46%), Gaps = 7/128 (5%)
Query 23 LDRLSELLVALVLCLIGF-IFARLVSNTFIRTIGQRFNAHQRM-----VWRRGIFYFIFL 76
+D L + + L +G+ + A L+ N I R R ++R + I
Sbjct 464 VDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRTLLTLFRNALAVIIST 523
Query 77 IFVMASLKEAGFKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGSFEVGDTIQIT 136
I +M L E G ++ L AG L +A+ F SQT ++I+G+F+ E GD + I
Sbjct 524 ITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGVFIQFENGMNTGDLVTIG 583
Query 137 LIRGHTIE 144
+ G T+E
Sbjct 584 PLTG-TVE 590
Score = 21.2 bits (43), Expect = 0.87
Identities = 16/49 (33%), Positives = 22/49 (45%), Gaps = 8/49 (16%)
Query 72 YFIFLIFVMASLKEAGFKLSVFLGAAGILTVALGFASQTSASNLISGLF 120
YFI L F SL + G L +GA +G A+ +SG+F
Sbjct 354 YFIVLFFF--SLFDPGNSLKFMMGATVRSLAIIGIAA------FVSGMF 394
>P0AAT4 Miniconductance mechanosensitive channel YbdG [Escherichia
coli (strain K12)]
Length=415
Score = 25.4 bits (54), Expect = 0.038
Identities = 41/192 (21%), Positives = 65/192 (34%), Gaps = 54/192 (28%)
Query 7 PKDVAVSLKGIFTNINLDRLSELLVALVLCLIGFIFARLVSNTFIRTIGQRFNAHQRMVW 66
P + LKGIF I LIG I
Sbjct 130 PAASQLPLKGIFQGIK--------------LIGAILV----------------------- 152
Query 67 RRGIFYFIFLIFVMASLKEAGFKLSVFLGAAGILTVALGFASQTSASNLISGLFLIGEGS 126
GI LI G ++ + G + L + L++G+ L
Sbjct 153 --GILMISLLI---------GQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDM 201
Query 127 FEVGDTIQITLIRGHTIEGQVISIDLLSVKLLTLDNVYVRLPNEQLIRAPVMN---MSKY 183
++GD +++ + +G VI I L +VK+ DN +P L+ N MS
Sbjct 202 LKLGDWLEMP---KYGADGAVIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSAS 258
Query 184 PIRRIPITLAIN 195
RRI +++I+
Sbjct 259 GGRRIKRSISID 270
Score = 19.6 bits (39), Expect = 2.5
Identities = 15/36 (42%), Positives = 18/36 (50%), Gaps = 2/36 (6%)
Query 90 LSVFLGAAGILTVALGF--ASQTSASNLISGLFLIG 123
LSVF IL +A F ASQ + G+ LIG
Sbjct 113 LSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIG 148
Lambda K H a alpha
0.327 0.142 0.407 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 2012310
Database: 3e9a53b82b8467eb2245b7db47094666.SwissProt.fasta
Posted date: May 19, 2024 11:06 PM
Number of letters in database: 8,780
Number of sequences in database: 19
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
