BLASTP 2.12.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.


Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.



Database: 8fb55286e49b166e6c54d2d03e30ea70.SwissProt.fasta
           17 sequences; 6,668 total letters



Query= Z3267

Length=267
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

P76422 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [Es...  528     0.0   
P55882 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [Sa...  485     3e-179
P56904 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [Rh...  254     5e-88 
P61422 Thiamine-phosphate synthase / Hydroxymethylpyrimidine/phos...  223     2e-73 
Q6GEY2 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [St...  206     5e-69 
Q8NVH3 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [St...  205     7e-69 
Q6G7L7 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [St...  205     7e-69 
P99124 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [St...  204     2e-68 
P66915 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [St...  204     2e-68 
Q5M731 Thiamine-phosphate synthase / Hydroxymethylpyrimidine kina...  205     4e-66 
O48881 Thiamine-phosphate synthase / Hydroxymethylpyrimidine kina...  199     1e-63 
Q8FTH8 Thiamine-phosphate synthase / Hydroxymethylpyrimidine/phos...  202     2e-63 
O31620 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [Ba...  187     5e-62 
P39610 Pyridoxine kinase [Bacillus subtilis (strain 168)]             163     1e-52 
Q08975 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI...  126     2e-36 
Q08224 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI...  122     5e-35 
Q06490 Thiamine biosynthesis protein THI22 [Saccharomyces cerevis...  112     3e-31 


>P76422 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 
[Escherichia coli (strain K12)]
Length=266

 Score = 528 bits (1359),  Expect = 0.0
 Identities = 265/266 (99%), Positives = 266/266 (100%), Gaps = 0/266 (0%)

Query  1    MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFV  60
            MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFV
Sbjct  1    MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFV  60

Query  61   AAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPS  120
            AAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPS
Sbjct  61   AAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPS  120

Query  121  AVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRALLAMGCGAVLMKGGHL  180
            AVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGR+LLAMGCGAVLMKGGHL
Sbjct  121  AVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRSLLAMGCGAVLMKGGHL  180

Query  181  DDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWL  240
            DDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWL
Sbjct  181  DDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWL  240

Query  241  SSALAQADTLEVGHGIGPVHHFHAWW  266
            SSALAQADTLEVGHGIGPVHHFHAWW
Sbjct  241  SSALAQADTLEVGHGIGPVHHFHAWW  266


>P55882 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 
[Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)]
Length=266

 Score = 485 bits (1248),  Expect = 3e-179
 Identities = 242/266 (91%), Positives = 254/266 (95%), Gaps = 0/266 (0%)

Query  1    MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFV  60
            M+RINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNT GVQSVYRIEPDFV
Sbjct  1    MQRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTCGVQSVYRIEPDFV  60

Query  61   AAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPS  120
            AAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQR+ ++NVVLDTVMLAKSGDPLLSPS
Sbjct  61   AAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRHHVRNVVLDTVMLAKSGDPLLSPS  120

Query  121  AVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRALLAMGCGAVLMKGGHL  180
            A+ TLR RLLPQVSLITPNLPEAAALLDAPHARTEQEML QGRALLAMGC AVLMKGGHL
Sbjct  121  AIETLRVRLLPQVSLITPNLPEAAALLDAPHARTEQEMLAQGRALLAMGCEAVLMKGGHL  180

Query  181  DDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWL  240
            +D QSPDWLFTREGEQRF+APR+ TKNTHGTGCTLSAALAALRPRH +W +TV EAK+WL
Sbjct  181  EDAQSPDWLFTREGEQRFSAPRVNTKNTHGTGCTLSAALAALRPRHRSWGETVNEAKAWL  240

Query  241  SSALAQADTLEVGHGIGPVHHFHAWW  266
            S+ALAQADTLEVG GIGPVHHFHAWW
Sbjct  241  SAALAQADTLEVGKGIGPVHHFHAWW  266


>P56904 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 
[Rhizobium meliloti (strain 1021)]
Length=266

 Score = 254 bits (648),  Expect = 5e-88
 Identities = 133/261 (51%), Positives = 175/261 (67%), Gaps = 1/261 (0%)

Query  6    ALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQLD  65
            AL+IAG+D  GGAGIQADLKTFSALG YG SVITA+ AQNTRGV +V  +  + V+AQ+D
Sbjct  5    ALSIAGSDSGGGAGIQADLKTFSALGVYGASVITAITAQNTRGVTAVEDVSAEIVSAQMD  64

Query  66   SVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSAVATL  125
            +VFSD+ +   KIGM++  + + A+A+ L+R+  +  V+D VM+A SGD LL P AVA L
Sbjct  65   AVFSDLDVKAVKIGMVSRRETIAAIADGLRRFG-KRAVVDPVMVATSGDALLRPDAVAAL  123

Query  126  RSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRALLAMGCGAVLMKGGHLDDEQS  185
               LLP   ++TPNL EAA +     A  E EM  Q  A++  G  AVL+KGGHL  +++
Sbjct  124  IEELLPLALVVTPNLAEAALMTGRAIAGDEAEMARQAEAIMRTGAHAVLVKGGHLKGQEA  183

Query  186  PDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWLSSALA  245
             D  F  +   R  A RI T+N HGTGCTLSAA+AA   +     + V  AK++L +A++
Sbjct  184  TDLFFDGDTLVRLPAGRIETRNDHGTGCTLSAAIAAGLAKGVPLIEAVSAAKAYLHAAIS  243

Query  246  QADTLEVGHGIGPVHHFHAWW  266
             AD LE+G G GPVHHFH WW
Sbjct  244  AADRLEIGQGRGPVHHFHRWW  264


>P61422 Thiamine-phosphate synthase / Hydroxymethylpyrimidine/phosphomethylpyrimidine 
kinase [Geobacter sulfurreducens (strain 
ATCC 51573 / DSM 12127 / PCA)]
Length=490

 Score = 223 bits (569),  Expect = 2e-73
 Identities = 122/262 (47%), Positives = 166/262 (63%), Gaps = 3/262 (1%)

Query  3    RINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAA  62
            R + LT+AG+D  GGAGIQADLKT + LG+YG SV+TAL AQNTRGV  ++ + P FVA 
Sbjct  225  RGSVLTVAGSDSGGGAGIQADLKTVTLLGSYGSSVLTALTAQNTRGVSGIHGVPPAFVAD  284

Query  63   QLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSAV  122
            QLD+VFSD+ +D  K GML   + + A+A +L  Y+ + VV+D VM+AK G  L+   AV
Sbjct  285  QLDAVFSDIPVDVVKTGMLFSAETIVAIAAKLTEYRRRMVVVDPVMVAKGGANLIDRGAV  344

Query  123  ATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRALLAMGCGAVLMKGGHLDD  182
            + L+ RL P   L+TPN+PEA  L  A +   E+ M E  R L  +G   VL+KGGHL  
Sbjct  345  SVLKERLFPLAYLVTPNIPEAERLTGA-NISDEESMREAARRLHRLGARNVLLKGGHLLA  403

Query  183  EQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWLSS  242
              S D LF      RF +PRI++KNTHGTGCT ++A+A    +     + +  AK ++++
Sbjct  404  GDSVDILFDGAAFHRFVSPRILSKNTHGTGCTFASAIATYLAQGDPLREAIARAKRYITA  463

Query  243  ALAQADTLEVGHGIGPVHHFHA  264
            A+  A  L  G G GPV+H  A
Sbjct  464  AIRLAQPL--GRGHGPVNHILA  483


>Q6GEY2 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 
[Staphylococcus aureus (strain MRSA252)]
Length=276

 Score = 206 bits (523),  Expect = 5e-69
 Identities = 112/259 (43%), Positives = 163/259 (63%), Gaps = 5/259 (2%)

Query  6    ALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQLD  65
            ALTIAGTDP+GGAG+ ADLK+F + G YG  V+T++VAQNT GVQ ++ +   +V  QLD
Sbjct  7    ALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQWVDEQLD  66

Query  66   SVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSAVATL  125
            SVF+D      K GM+A  D +E +   L +++    V+D VMLAKSGD L+  +    L
Sbjct  67   SVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDNNTKQNL  126

Query  126  RSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRALL-AMGCGAVLMKGGHLDD-E  183
            +  LLP   ++TPNLPEA  +       +E+++++ GR  +  +G   V++KGGH +D +
Sbjct  127  QHTLLPLADVVTPNLPEAEEITGLT-IDSEEKIMQAGRIFINEIGSKGVIIKGGHSNDAD  185

Query  184  QSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWLSSA  243
             + D+LFT+EG Q F   R  TK+THGTGCT SA + A   +     + V +AK ++S +
Sbjct  186  IAKDYLFTKEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRPLFEAVHKAKKFISMS  245

Query  244  LAQADTLEVGHGIGPVHHF  262
            +    T E+G G GPV+HF
Sbjct  246  IQY--TPEIGRGRGPVNHF  262


>Q8NVH3 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 
[Staphylococcus aureus (strain MW2)]
Length=276

 Score = 205 bits (522),  Expect = 7e-69
 Identities = 112/259 (43%), Positives = 161/259 (62%), Gaps = 5/259 (2%)

Query  6    ALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQLD  65
            ALTIAGTDP+GGAG+ ADLK+F + G YG  V+T++VAQNT GVQ ++ +   +V  QLD
Sbjct  7    ALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQWVDEQLD  66

Query  66   SVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSAVATL  125
            SVF+D      K GM+A  D +E +   L +++    V+D VMLAKSGD L+       L
Sbjct  67   SVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDNDTKQNL  126

Query  126  RSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRALL-AMGCGAVLMKGGHLDD-E  183
            +  LLP   ++TPNLPEA  +       +E+++++ GR  +  +G   V++KGGH +D +
Sbjct  127  QHTLLPLADVVTPNLPEAEEITGLT-IDSEEKIMQAGRIFINEIGSKGVIIKGGHSNDTD  185

Query  184  QSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWLSSA  243
             + D+LFT EG Q F   R  TK+THGTGCT SA + A   +     + V +AK ++S +
Sbjct  186  IAKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRRLFEAVHKAKKFISMS  245

Query  244  LAQADTLEVGHGIGPVHHF  262
            +    T E+G G GPV+HF
Sbjct  246  IQY--TPEIGRGRGPVNHF  262


>Q6G7L7 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 
[Staphylococcus aureus (strain MSSA476)]
Length=276

 Score = 205 bits (522),  Expect = 7e-69
 Identities = 112/259 (43%), Positives = 161/259 (62%), Gaps = 5/259 (2%)

Query  6    ALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQLD  65
            ALTIAGTDP+GGAG+ ADLK+F + G YG  V+T++VAQNT GVQ ++ +   +V  QLD
Sbjct  7    ALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQWVDEQLD  66

Query  66   SVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSAVATL  125
            SVF+D      K GM+A  D +E +   L +++    V+D VMLAKSGD L+       L
Sbjct  67   SVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDNDTKQNL  126

Query  126  RSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRALL-AMGCGAVLMKGGHLDD-E  183
            +  LLP   ++TPNLPEA  +       +E+++++ GR  +  +G   V++KGGH +D +
Sbjct  127  QHTLLPLADVVTPNLPEAEEITGLT-IDSEEKIMQAGRIFINEIGSKGVIIKGGHSNDTD  185

Query  184  QSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWLSSA  243
             + D+LFT EG Q F   R  TK+THGTGCT SA + A   +     + V +AK ++S +
Sbjct  186  IAKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRRLFEAVHKAKKFISMS  245

Query  244  LAQADTLEVGHGIGPVHHF  262
            +    T E+G G GPV+HF
Sbjct  246  IQY--TPEIGRGRGPVNHF  262


>P99124 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 
[Staphylococcus aureus (strain N315)]
Length=276

 Score = 204 bits (519),  Expect = 2e-68
 Identities = 111/259 (43%), Positives = 161/259 (62%), Gaps = 5/259 (2%)

Query  6    ALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQLD  65
            ALTIAGTDP+GGAG+ ADLK+F + G YG  V+T++VAQNT GVQ ++ +   +V  QLD
Sbjct  7    ALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQWVDEQLD  66

Query  66   SVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSAVATL  125
            SVF+D      K GM+A  D +E +   L +++    V+D VMLAKSGD L+       L
Sbjct  67   SVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDNDTKQNL  126

Query  126  RSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRALL-AMGCGAVLMKGGHLDD-E  183
            +  LLP   ++TPNLPEA  +       +E+++++ GR  +  +G   +++KGGH +D +
Sbjct  127  QHTLLPLADVVTPNLPEAEEITGLT-IDSEEKIMQAGRIFINEIGSKGIIIKGGHSNDTD  185

Query  184  QSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWLSSA  243
             + D+LFT EG Q F   R  TK+THGTGCT SA + A   +     + V +AK ++S +
Sbjct  186  IAKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRPLFEAVHKAKKFISMS  245

Query  244  LAQADTLEVGHGIGPVHHF  262
            +    T E+G G GPV+HF
Sbjct  246  IQY--TPEIGRGRGPVNHF  262


>P66915 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 
[Staphylococcus aureus (strain Mu50 / ATCC 700699)]
Length=276

 Score = 204 bits (519),  Expect = 2e-68
 Identities = 111/259 (43%), Positives = 161/259 (62%), Gaps = 5/259 (2%)

Query  6    ALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQLD  65
            ALTIAGTDP+GGAG+ ADLK+F + G YG  V+T++VAQNT GVQ ++ +   +V  QLD
Sbjct  7    ALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQWVDEQLD  66

Query  66   SVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSAVATL  125
            SVF+D      K GM+A  D +E +   L +++    V+D VMLAKSGD L+       L
Sbjct  67   SVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDNDTKQNL  126

Query  126  RSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRALL-AMGCGAVLMKGGHLDD-E  183
            +  LLP   ++TPNLPEA  +       +E+++++ GR  +  +G   +++KGGH +D +
Sbjct  127  QHTLLPLADVVTPNLPEAEEITGLT-IDSEEKIMQAGRIFINEIGSKGIIIKGGHSNDTD  185

Query  184  QSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWLSSA  243
             + D+LFT EG Q F   R  TK+THGTGCT SA + A   +     + V +AK ++S +
Sbjct  186  IAKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRPLFEAVHKAKKFISMS  245

Query  244  LAQADTLEVGHGIGPVHHF  262
            +    T E+G G GPV+HF
Sbjct  246  IQY--TPEIGRGRGPVNHF  262


>Q5M731 Thiamine-phosphate synthase / Hydroxymethylpyrimidine 
kinase [Arabidopsis thaliana]
Length=522

 Score = 205 bits (522),  Expect = 4e-66
 Identities = 115/258 (45%), Positives = 159/258 (62%), Gaps = 2/258 (1%)

Query  7    LTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQLDS  66
            LT+AG+D   GAGIQADLK  +A G Y  SVITA+ AQNTRGVQSV+ + P+F++ QL S
Sbjct  34   LTVAGSDSGAGAGIQADLKVCAARGVYCASVITAVTAQNTRGVQSVHLLPPEFISEQLKS  93

Query  67   VFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSAVATLR  126
            V SD   D  K GML  T+IVE + + L  + ++ +V+D VM++ SG  L   S ++  R
Sbjct  94   VLSDFEFDVVKTGMLPSTEIVEVLLQNLSDFPVRALVVDPVMVSTSGHVLAGSSILSIFR  153

Query  127  SRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRALLAMGCGAVLMKGGHL-DDEQS  185
             RLLP   +ITPN+ EA+ALLD     T  EM    ++L  MG   VL+KGG L D   S
Sbjct  154  ERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLPDSSDS  213

Query  186  PDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWLSSALA  245
             D  F  +      +PRI T+NTHGTGCTL++ +AA   + ++    V+ AK ++ +AL 
Sbjct  214  VDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSMLSAVKVAKRFVDNALD  273

Query  246  QADTLEVGHGI-GPVHHF  262
             +  + +G G+ GP  HF
Sbjct  274  YSKDIVIGSGMQGPFDHF  291


>O48881 Thiamine-phosphate synthase / Hydroxymethylpyrimidine 
kinase [Brassica napus]
Length=523

 Score = 199 bits (505),  Expect = 1e-63
 Identities = 114/263 (43%), Positives = 156/263 (59%), Gaps = 2/263 (1%)

Query  2    KRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVA  61
            K    LT+AG+D   GAGIQAD+K  +A G Y  SV TA+ A+NTR VQSV+ + PD V+
Sbjct  31   KVAQVLTVAGSDSGAGAGIQADIKVCAARGVYCASVKTAVKAKNTRAVQSVHLLPPDSVS  90

Query  62   AQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSA  121
             QL SV SD  +D  K GML   +IVE + + L  Y ++ +V+D VM++ SG  L   S 
Sbjct  91   EQLKSVLSDFEVDVVKTGMLPSPEIVEVLLQNLSEYPVRALVVDPVMVSTSGHVLAGSSI  150

Query  122  VATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRALLAMGCGAVLMKGGHL-  180
            ++  R RLLP   +ITPN+ EA+ALL     +T  EM    ++L  MG   VL+KGG L 
Sbjct  151  LSIFRERLLPLADIITPNVKEASALLGGVRIQTVAEMRSAAKSLHQMGPRFVLVKGGDLP  210

Query  181  DDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWL  240
            D   S D  F         +PRI T+NTHGTGCTL++ +AA   + +N    V+ AK ++
Sbjct  211  DSSDSVDVYFDGNEFHELHSPRIATRNTHGTGCTLASCIAAELAKGSNMLSAVKVAKRFV  270

Query  241  SSALAQADTLEVGHGI-GPVHHF  262
             SAL  +  + +G G+ GP  HF
Sbjct  271  DSALNYSKDIVIGSGMQGPFDHF  293


>Q8FTH8 Thiamine-phosphate synthase / Hydroxymethylpyrimidine/phosphomethylpyrimidine 
kinase [Corynebacterium efficiens (strain 
DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)]
Length=739

 Score = 202 bits (514),  Expect = 2e-63
 Identities = 120/257 (47%), Positives = 150/257 (58%), Gaps = 2/257 (1%)

Query  7    LTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQLDS  66
            LTIAGTDP+GGAG+QADLK+ +A G +G SVITALVAQNT GV  V+    DF+  QL+S
Sbjct  226  LTIAGTDPTGGAGVQADLKSIAAAGGFGMSVITALVAQNTHGVTGVHTPPADFLDEQLES  285

Query  67   VFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSAVATLR  126
            VFSDV +D  K+GML   D V  V   L+      V+LD VM+A SGD LL P A   L 
Sbjct  286  VFSDVTVDAVKLGMLGRADTVRQVTGWLRTRPHGPVILDPVMVATSGDSLLDPDATEAL-  344

Query  127  SRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRALLAMGCGAVLMKGGHLDDEQSP  186
              L   V +ITPN+PE A L     A +    +EQ R         V++KGGHL   ++ 
Sbjct  345  LELATVVDVITPNIPELAVLCGEQPAPSFDAAIEQARRFATDVGTTVIVKGGHLTGPRAD  404

Query  187  DWLFTREGEQRFTA-PRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWLSSALA  245
            + +   +G     A PR+ T N+HGTGC+LSAALA            +  A  WL+ AL 
Sbjct  405  NAVVYPDGSVHMVANPRVDTTNSHGTGCSLSAALATRMGAGHPVDKALDWATRWLNEALR  464

Query  246  QADTLEVGHGIGPVHHF  262
             AD L+VG G GPV HF
Sbjct  465  GADALQVGSGSGPVDHF  481


 Score = 19.6 bits (39),  Expect = 1.7
 Identities = 10/25 (40%), Positives = 13/25 (52%), Gaps = 1/25 (4%)

Query  171  GAVLMKGGHLDDEQSPDWLFTREGE  195
            G +L K  H +   + DWL T  GE
Sbjct  664  GLILAKQNHPEHPYT-DWLDTYSGE  687


>O31620 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 
[Bacillus subtilis (strain 168)]
Length=271

 Score = 187 bits (476),  Expect = 5e-62
 Identities = 110/265 (42%), Positives = 154/265 (58%), Gaps = 8/265 (3%)

Query  1    MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFV  60
            M    ALTIAG+D  GGAGIQAD+KTF  L  +G S ITA+ AQNT GV  V+ +  + +
Sbjct  1    MSIYKALTIAGSDSGGGAGIQADIKTFQELDVFGMSAITAVTAQNTLGVHGVHPLTVETL  60

Query  61   AAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPS  120
              Q+D+V  D+R D  K GML   D++E VA ++  Y    V++D VM+AK G  LL   
Sbjct  61   RQQIDAVAEDLRPDAVKTGMLWNADMIEEVARKIDEYGFNRVIVDPVMIAKGGASLLRDE  120

Query  121  AVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRALLAMGCGAVLMKGGHL  180
            +VATL+  L+P+   ITPN+PEA  L     +  + +  +    L+ MG   V++KGGH 
Sbjct  121  SVATLKELLIPRSYAITPNVPEAETLTGMTISSLD-DRKKAAEQLVKMGAQHVIIKGGHQ  179

Query  181  -DDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSW  239
             +D    D LF      + T P I TK+THGTGCT +AAL A     T   D++ +A   
Sbjct  180  PEDNHITDLLFDGSMFMQITHPYINTKHTHGTGCTFAAALTA----QTAKGDSIHQAFEV  235

Query  240  LSSALAQA--DTLEVGHGIGPVHHF  262
             ++ + +A  +TL +G G GP +HF
Sbjct  236  AANFVREAVENTLGIGSGHGPTNHF  260


>P39610 Pyridoxine kinase [Bacillus subtilis (strain 168)]
Length=271

 Score = 163 bits (413),  Expect = 1e-52
 Identities = 95/264 (36%), Positives = 145/264 (55%), Gaps = 5/264 (2%)

Query  1    MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ--NTRGVQSVYRIEPD  58
            M    ALTIAG+D SGGAGIQADLKTF     YG + +T +VA   N      V+ I+ D
Sbjct  1    MSMHKALTIAGSDSSGGAGIQADLKTFQEKNVYGMTALTVIVAMDPNNSWNHQVFPIDTD  60

Query  59   FVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLS  118
             + AQL ++   + +D  K GML   DI+E  A+ ++  Q++NVV+D VM+ K  + +L 
Sbjct  61   TIRAQLATITDGIGVDAMKTGMLPTVDIIELAAKTIKEKQLKNVVIDPVMVCKGANEVLY  120

Query  119  PSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRALLAMGCG-AVLMKG  177
            P     LR +L P  ++ITPNL EA+ L      +T  +M+E  + + A+G    V+  G
Sbjct  121  PEHAQALREQLAPLATVITPNLFEASQLSGMDELKTVDDMIEAAKKIHALGAQYVVITGG  180

Query  178  GHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAK  237
            G L  E++ D L+  E  +   +  I T  THG GCT SAA+ A   +     + +  AK
Sbjct  181  GKLKHEKAVDVLYDGETAEVLESEMIDTPYTHGAGCTFSAAVTAELAKGAEVKEAIYAAK  240

Query  238  SWLSSALAQADTLEVGHGIGPVHH  261
             ++++A+   ++  +   +GP  H
Sbjct  241  EFITAAI--KESFPLNQYVGPTKH  262


>Q08975 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 
THI21 [Saccharomyces cerevisiae (strain ATCC 204508 / S288c)]
Length=551

 Score = 126 bits (317),  Expect = 2e-36
 Identities = 91/272 (33%), Positives = 147/272 (54%), Gaps = 16/272 (6%)

Query  7    LTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQLDS  66
            L+IAGTDPSGGAG++AD+KT +A   Y  + ITAL AQ    V S+     + V+  LD+
Sbjct  27   LSIAGTDPSGGAGVEADVKTITAHRCYAMTCITALNAQTPVKVYSINNTPKEVVSQILDA  86

Query  67   VFSDVRIDTTKIGMLAETDIVEAVAERLQRY--QIQNVVLDTVMLAKSGDPLLSPSAVAT  124
               D++ D  K GML  T  +E + E+L +       +V+D V++A SG  L      + 
Sbjct  87   NLQDMKCDVIKTGMLT-TAAIEVLHEKLLQLGENRPKLVVDPVLVATSGSSLAGKDIASL  145

Query  125  LRSRLLPQVSLITPNLPEAAALLDAPHARTE-QEMLEQGRALLAM-GCGAVLMKGGHL--  180
            +  ++ P   ++TPN+PE   LL      ++ +++ E  + L  +  C  +L+KGGH+  
Sbjct  146  ITEKIAPFADILTPNIPECFKLLGEDREISKLRDIFEVAKDLAKITKCSNILVKGGHIPW  205

Query  181  DDEQS---PDWLFTREGEQRFTAPR---IMTKNTHGTGCTLSAALAALRPRHTNWADTVQ  234
            +DE+     D L+    EQRF   +   + T +THGTGCTL++A+A+   R  +   +V 
Sbjct  206  NDEEGKYITDVLYL-GAEQRFITFKGNFVNTTHTHGTGCTLASAIASNLARGYSLPQSVY  264

Query  235  EAKSWLSSALAQA--DTLEVGHGIGPVHHFHA  264
                ++ +A+A     T E     GP++H +A
Sbjct  265  GGIEYVQNAVAIGCDVTKETVKDNGPINHVYA  296


>Q08224 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 
THI20 [Saccharomyces cerevisiae (strain ATCC 204508 / S288c)]
Length=551

 Score = 122 bits (306),  Expect = 5e-35
 Identities = 91/272 (33%), Positives = 145/272 (53%), Gaps = 16/272 (6%)

Query  7    LTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQLDS  66
            L+IAGTDPSGGAGI+AD+KT +A   Y  + ITAL AQ    V S+     + V   L+S
Sbjct  27   LSIAGTDPSGGAGIEADVKTITAHRCYAMTCITALNAQTPVKVYSINNTPKEVVFQTLES  86

Query  67   VFSDVRIDTTKIGMLAETDIVEAVAERLQRY--QIQNVVLDTVMLAKSGDPLLSPSAVAT  124
               D++ +  K GML     +E + E+L +       +V+D V++A SG  L     V+ 
Sbjct  87   NLKDMKCNVIKTGMLTAA-AIEVLHEKLLQLGENRPKLVVDPVLVATSGSSLAGKDIVSL  145

Query  125  LRSRLLPQVSLITPNLPEAAALL-DAPHARTEQEMLEQGRALLAM-GCGAVLMKGGHL--  180
            +  ++ P   ++TPN+PE   LL +       Q++ +  + L  +  C  +L+KGGH+  
Sbjct  146  ITEKVAPFADILTPNIPECYKLLGEERKVNGLQDIFQIAKDLAKITKCSNILVKGGHIPW  205

Query  181  DDEQS---PDWLFTREGEQRFTAPR---IMTKNTHGTGCTLSAALAALRPRHTNWADTVQ  234
            +DE+     D LF    EQ+F   +   + T +THGTGCTL++A+A+   R  +   +V 
Sbjct  206  NDEKEKYITDVLFL-GAEQKFIIFKGNFVNTTHTHGTGCTLASAIASNLARGYSLPQSVY  264

Query  235  EAKSWLSSALAQA--DTLEVGHGIGPVHHFHA  264
                ++ +A+A     T E     GP++H +A
Sbjct  265  GGIEYVQNAVAIGCDVTKETVKDNGPINHVYA  296


>Q06490 Thiamine biosynthesis protein THI22 [Saccharomyces cerevisiae 
(strain ATCC 204508 / S288c)]
Length=572

 Score = 112 bits (279),  Expect = 3e-31
 Identities = 78/270 (29%), Positives = 137/270 (51%), Gaps = 13/270 (5%)

Query  7    LTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQLDS  66
            ++IAG+D SGGAG++AD+KT +A   Y  + +T L AQ    V     I    V+  LD+
Sbjct  49   MSIAGSDSSGGAGVEADIKTITAHRCYAMTCVTTLTAQTPVKVYGAQNIPKKMVSQILDA  108

Query  67   VFSDVRIDTTKIGMLAETDIVEAVAERLQRY--QIQNVVLDTVMLAKSGDPLLSPSAVAT  124
               D++ +  K GML   D +E + E+L +       +V+D V+ A S         V+ 
Sbjct  109  NLQDMKCNVIKTGMLT-VDAIEVLHEKLLQLGENRPKLVIDPVLCAASDSSPTGKDVVSL  167

Query  125  LRSRLLPQVSLITPNLPEAAALLDAPHARTE-QEMLEQGRALLAM-GCGAVLMKGGHLDD  182
            +  ++ P   ++TPN+ +   LL      ++ Q++LE  + L  +  C  +L+KGGH+  
Sbjct  168  IIEKISPFADILTPNISDCFMLLGENREVSKLQDVLEIAKDLSRITNCSNILVKGGHIPC  227

Query  183  EQSPDWLFT----REGEQRFTAPR---IMTKNTHGTGCTLSAALAALRPRHTNWADTVQE  235
            +   +   T       EQ+F   +   + T  THG GCTL++A+A+   R  + + +V  
Sbjct  228  DDGKEKHITDVLYLGAEQKFITFKGQFVNTTRTHGAGCTLASAIASNLARGYSLSQSVYG  287

Query  236  AKSWLSSALA-QADTLEVGHGIGPVHHFHA  264
               ++ +A+A   D  +    +GP++H +A
Sbjct  288  GIEYVQNAIAIGCDVTKKAVKVGPINHVYA  317



Lambda      K        H        a         alpha
   0.320    0.133    0.396    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 1291180


  Database: 8fb55286e49b166e6c54d2d03e30ea70.SwissProt.fasta
    Posted date:  Jun 2, 2024  3:53 AM
  Number of letters in database: 6,668
  Number of sequences in database:  17



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40