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Prior-Knowledge Description Expectation Prediction Conclusion Leaf Statistics
UER00069 2-oxobutanoate from pyruvate: step 3/3~1 D-erythro-3-methylmalic acid + 1 NAD(+) => 1 2-oxobutanoate + 1 CO(2) + 1 H(+) + 1 NADH. None - {{∅}} None - {{∅}} Unexplained
UCR02199 2-oxoglutarate + L-isoleucine = (S)-3-methyl-2-oxopentanoate + L-glutamate True - {{t}} True - {{t}} Confirmed presence
UCR07399 H(2)O + acetyl-CoA + pyruvate = (R)-citramalate + CoA None - {{∅}} None - {{∅}} Unexplained
ULS00019 2-oxobutanoate from pyruvate None - {{∅}} None - {{∅}} Unexplained
UCR00994 2-oxobutanoate + CO(2) + H(+) + NADH = D-erythro-3-methylmalic acid + NAD(+) None - {{∅}} None - {{∅}} Unexplained
MUER17 Meta-UER 17~1 L-threonine => 1 2-oxobutanoate + 1 NH(3). True - {{t}} True - {{t}} Confirmed presence
UCR03898 2-methylmaleate + H(2)O = D-erythro-3-methylmalic acid None - {{∅}} None - {{∅}} Unexplained
UCR04673 2-hydroxyethyl-ThPP + 2-oxobutanoate = (S)-2-acetyl-2-hydroxybutanoic acid + thiamine diphosphate True - {{t}} None - {{∅}} Missing
ULS00020 L-isoleucine from 2-oxobutanoate True - {{∅},{t}} True - {{t}} Confirmed presence
UER00058 L-isoleucine from 2-oxobutanoate: step 4/4~1 (S)-3-methyl-2-oxopentanoate + 1 L-glutamate => 1 2-oxoglutarate + 1 L-isoleucine. True - {{t}} True - {{t}} Confirmed presence
ULS00018 2-oxobutanoate from L-threonine True - {{t}} True - {{t}} Confirmed presence
UER00067 2-oxobutanoate from pyruvate: step 2/3~1 (R)-citramalate => 1 D-erythro-3-methylmalic acid. None - {{∅}} None - {{∅}} Unexplained
UER00057 L-isoleucine from 2-oxobutanoate: step 3/4~1 (2R,3R)-2,3-dihydroxy-3-methylpentanoic acid => 1 (S)-3-methyl-2-oxopentanoate + 1 H(2)O. True - {{t}} True - {{t}} Confirmed presence
UCR00996 L-threonine = 2-oxobutanoate + NH(3) True - {{t}} True - {{t}} Confirmed presence
UER00056 L-isoleucine from 2-oxobutanoate: step 2/4~1 (S)-2-acetyl-2-hydroxybutanoic acid + 1 H(+) + 1 NADPH => 1 (2R,3R)-2,3-dihydroxy-3-methylpentanoic acid + 1 NADP(+). True - {{t}} True - {{t}} Confirmed presence
UCR05070 (2R,3R)-2,3-dihydroxy-3-methylpentanoic acid = (S)-3-methyl-2-oxopentanoate + H(2)O True - {{t}} True - {{t}} Confirmed presence
UCR05069 (S)-2-acetyl-2-hydroxybutanoic acid = (R)-3-hydroxy-3-methyl-2-oxopentanoate True - {{t}} None - {{∅}} Missing
UER00055 L-isoleucine from 2-oxobutanoate: step 1/4~1 2-hydroxyethyl-ThPP + 1 2-oxobutanoate => 1 (S)-2-acetyl-2-hydroxybutanoic acid + 1 thiamine diphosphate. True - {{t}} True - {{t}} Confirmed presence
VariantPath-2-UPA00047 True - {{t}} True - {{t}} Confirmed presence
VariantPath-1-UPA00047 None - {{∅}} None - {{∅},{t}} Unexplained
UPA00047 L-isoleucine biosynthesis~Biosynthesis of L-isoleucine, (2R*,3R*)-2-amino-3-methylpentanoic acid, an hydrophobic branched- chain amino-acid. In most microorganisms, isoleucine is synthesized from aspartate via threonine [Umbarger, H. E. 1978. Amino-acid biosynthesis and its regulation. Annu. Rev. Biochem. 47:533-606]. However, alternative routes to isoleucine from precursors other than threonine have been reported. Some anaerobes can assimilate 2-methylbutyrate into isoleucine. The most commonly observed alternative route was a route from pyruvate and acetyl coenzyme A (acetyl-CoA) via citramalate. This 'pyruvate pathway' was initially proposed for the genus Leptospira because isotope-labeling experiments indicated that in some leptospiral strains, {alpha}-ketobutyrate was derived from pyruvate rather than threonine. Only a limited number of leptospires possess catabolic threonine dehydratase. Later, a similar observation was made with a thermophilic archaeon, Methanobacterium thermoautotrophicum, suggesting that isoleucine biosynthesis involves pyruvate as a precursor. Recently, (R)-citramalate synthase (EC 4.1.3.-) activity was demonstrated in the thermophilic archaeon Methanococcus jannaschii. True - {{t}} True - {{t}} Confirmed presence
UCR03896 (R)-citramalate = 2-methylmaleate + H(2)O None - {{∅}} None - {{∅}} Unexplained
UER00066 2-oxobutanoate from pyruvate: step 1/3~1 H(2)O + 1 acetyl-CoA + 1 pyruvate => 1 (R)-citramalate + 1 CoA. None - {{∅}} None - {{∅}} Unexplained
UCR05068 (2R,3R)-2,3-dihydroxy-3-methylpentanoic acid + NADP(+) = (R)-3-hydroxy-3-methyl-2-oxopentanoate + H(+) + NADPH True - {{t}} None - {{∅}} Missing