| UER00069 |
2-oxobutanoate from pyruvate: step 3/3~1 D-erythro-3-methylmalic acid + 1 NAD(+) => 1 2-oxobutanoate + 1 CO(2) + 1 H(+) + 1 NADH. |
None - {{∅}} |
None - {{∅}} |
Unexplained |
|
| UCR02199 |
2-oxoglutarate + L-isoleucine = (S)-3-methyl-2-oxopentanoate + L-glutamate |
True - {{∅},{t}} |
True - {{t}} |
Confirmed presence |
|
| UCR07399 |
H(2)O + acetyl-CoA + pyruvate = (R)-citramalate + CoA |
None - {{∅}} |
None - {{∅}} |
Unexplained |
|
| ULS00019 |
2-oxobutanoate from pyruvate |
None - {{∅}} |
None - {{∅}} |
Unexplained |
|
| UCR00994 |
2-oxobutanoate + CO(2) + H(+) + NADH = D-erythro-3-methylmalic acid + NAD(+) |
None - {{∅}} |
None - {{∅}} |
Unexplained |
|
| MUER17 |
Meta-UER 17~1 L-threonine => 1 2-oxobutanoate + 1 NH(3). |
True - {{∅},{t}} |
True - {{t}} |
Confirmed presence |
|
| UCR03898 |
2-methylmaleate + H(2)O = D-erythro-3-methylmalic acid |
None - {{∅}} |
None - {{∅}} |
Unexplained |
|
| UCR04673 |
2-hydroxyethyl-ThPP + 2-oxobutanoate = (S)-2-acetyl-2-hydroxybutanoic acid + thiamine diphosphate |
True - {{∅},{t}} |
None - {{∅}} |
Missing |
|
| ULS00020 |
L-isoleucine from 2-oxobutanoate |
True - {{∅},{t}} |
True - {{t}} |
Confirmed presence |
|
| UER00058 |
L-isoleucine from 2-oxobutanoate: step 4/4~1 (S)-3-methyl-2-oxopentanoate + 1 L-glutamate => 1 2-oxoglutarate + 1 L-isoleucine. |
True - {{∅},{t}} |
True - {{t}} |
Confirmed presence |
|
| ULS00018 |
2-oxobutanoate from L-threonine |
True - {{t}} |
True - {{t}} |
Confirmed presence |
|
| UER00067 |
2-oxobutanoate from pyruvate: step 2/3~1 (R)-citramalate => 1 D-erythro-3-methylmalic acid. |
None - {{∅}} |
None - {{∅}} |
Unexplained |
|
| UER00057 |
L-isoleucine from 2-oxobutanoate: step 3/4~1 (2R,3R)-2,3-dihydroxy-3-methylpentanoic acid => 1 (S)-3-methyl-2-oxopentanoate + 1 H(2)O. |
True - {{∅},{t}} |
True - {{t}} |
Confirmed presence |
|
| UCR00996 |
L-threonine = 2-oxobutanoate + NH(3) |
True - {{∅},{t}} |
True - {{t}} |
Confirmed presence |
|
| UER00056 |
L-isoleucine from 2-oxobutanoate: step 2/4~1 (S)-2-acetyl-2-hydroxybutanoic acid + 1 H(+) + 1 NADPH => 1 (2R,3R)-2,3-dihydroxy-3-methylpentanoic acid + 1 NADP(+). |
True - {{∅},{t}} |
True - {{t}} |
Confirmed presence |
|
| UCR05070 |
(2R,3R)-2,3-dihydroxy-3-methylpentanoic acid = (S)-3-methyl-2-oxopentanoate + H(2)O |
True - {{∅},{t}} |
True - {{t}} |
Confirmed presence |
|
| UCR05069 |
(S)-2-acetyl-2-hydroxybutanoic acid = (R)-3-hydroxy-3-methyl-2-oxopentanoate |
True - {{∅},{t}} |
None - {{∅}} |
Missing |
|
| UER00055 |
L-isoleucine from 2-oxobutanoate: step 1/4~1 2-hydroxyethyl-ThPP + 1 2-oxobutanoate => 1 (S)-2-acetyl-2-hydroxybutanoic acid + 1 thiamine diphosphate. |
True - {{∅},{t}} |
None - {{∅}} |
Missing |
|
| VariantPath-2-UPA00047 |
|
True - {{t}} |
True - {{t}} |
Confirmed presence |
|
| VariantPath-1-UPA00047 |
|
None - {{∅}} |
None - {{∅},{t}} |
Unexplained |
|
| UPA00047 |
L-isoleucine biosynthesis~Biosynthesis of L-isoleucine, (2R*,3R*)-2-amino-3-methylpentanoic acid, an hydrophobic branched- chain amino-acid. In most microorganisms, isoleucine is synthesized from aspartate via threonine [Umbarger, H. E. 1978. Amino-acid biosynthesis and its regulation. Annu. Rev. Biochem. 47:533-606]. However, alternative routes to isoleucine from precursors other than threonine have been reported. Some anaerobes can assimilate 2-methylbutyrate into isoleucine. The most commonly observed alternative route was a route from pyruvate and acetyl coenzyme A (acetyl-CoA) via citramalate. This 'pyruvate pathway' was initially proposed for the genus Leptospira because isotope-labeling experiments indicated that in some leptospiral strains, {alpha}-ketobutyrate was derived from pyruvate rather than threonine. Only a limited number of leptospires possess catabolic threonine dehydratase. Later, a similar observation was made with a thermophilic archaeon, Methanobacterium thermoautotrophicum, suggesting that isoleucine biosynthesis involves pyruvate as a precursor. Recently, (R)-citramalate synthase (EC 4.1.3.-) activity was demonstrated in the thermophilic archaeon Methanococcus jannaschii. |
True - {{t}} |
True - {{t}} |
Confirmed presence |
|
| UCR03896 |
(R)-citramalate = 2-methylmaleate + H(2)O |
None - {{∅}} |
None - {{∅}} |
Unexplained |
|
| UER00066 |
2-oxobutanoate from pyruvate: step 1/3~1 H(2)O + 1 acetyl-CoA + 1 pyruvate => 1 (R)-citramalate + 1 CoA. |
None - {{∅}} |
None - {{∅}} |
Unexplained |
|
| UCR05068 |
(2R,3R)-2,3-dihydroxy-3-methylpentanoic acid + NADP(+) = (R)-3-hydroxy-3-methyl-2-oxopentanoate + H(+) + NADPH |
True - {{∅},{t}} |
None - {{∅}} |
Missing |
|