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Sus scrofa

Sequencing BAC 493A6

Project: Collaborative
State of the project: Completed



Collaborations :
Laboratoire de Radiobiologie et Etude du Génome
INRA CEA
Jouy en Josas - France
Access to :
Access to the sequence of 493A6 BAC: Accession Number AJ251914

A segment of 158,063 nucleotides of the pig major histocompatibility complex (SLA) and corresponding to the junction of the class I and class III regions was sequenced entirely using Sanger method.

1,763 reads of subclones ends were run on a LICOR 4200 sequencer and the sequences were assembled into contigs using Phred and Phrap software. Gaps between contigs were filled and sequenced by walking, and poor quality sequences were determined using 116 primers and dye terminators on an automated ABI 377 sequencer.

Contacts : Laurence Cattolico (Genoscope) - Patrick Chardon (INRA CEA)

Last update on 11 June 2009

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