Mycoplasma mycoides ssp. mycoides LC

Genoscope is undertaking the random shotgun sequencing of the genome of Mycoplasma mycoides ssp mycoides LC, for which the genome is estimated at 1.2 Mb.
The genetic material to be used is from a clinical case of mammitis of the goat, from which the MmmLC strain was isolated in pure culture.

Sequencing will be carried out using 3 plasmid libraries :

• 7,000 inserts of 3 kb cloned in the multicopy plasmid pcdna2.1,
• 2,500 inserts of 10 kb cloned in the small copy number plasmid Pcns and about 800 inserts of 20-25 kb cloned in the pBBC vector.

A total of 24,000 reads are planned to obtain a coverage of about 12X.
A finishing step will be performed at Genoscope.

Contacts:
Valérie Barbe (Genoscope) - François Thiaucourt (CIRAD)