Mycoplasma agalactiae PG2 and 5632

The genomes of the PG2 and 5632 strains of Mycoplasma agalactiae each have a size of about 950 kb with a G+C% of about 30%. Genoscope will perform about 14,000 reads for each genome, in order to obtain a depth of 10X, with an average read length of 650 bps. These reads are carried out using two types of libraries:

1. Some prepared from products of mechanical shearing of the genome (HydroSHear apparatus): one library from fragments of about 3 kb cloned in the pcdna2.1 vector (5,000 clones sequenced on both ends) and another from fragments of about 10 kb cloned in the pcns vector (1,000 clones sequenced on both ends).
2. Another library whose inserts were obtained by partial digestion of each genome by Sau3A. The fragments, of about 20 kb, were cloned in the pBBc miniBAC vector; 1,000 clones from this library will be sequenced on both extremities. The clone links produced by these sequences will be useful in the assembly phase. The finishing phases will produce a quality sequence which conforms to the Bermuda rules.

Contact: Valérie Barbe (Genoscope) - Marc Marenda (ENVT)