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State of the project:
Laboratoire de Microbiologie et EA 3647
Faculté de Médecine Paris Ile-de-France Ouest
Laboratoire Génome et Informatique,
UMR CNRS 8116
CNRS/ Université d’Evry
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M. abscessus is a recently recognized pulmonary pathogen in cystic fibrosis that is susceptible to only a few drugs and is responsible for a severe pseudo-tuberculous disease. By substractive hybridization between M. abscessus (CIP 104536T) and the closely-related species, M. chelonae (CIP 104535T), we have recently identified M. abscessus-specific ORFs homologous to genes involved in the pathogenicity of M. tuberculosis, including members of the pks family. This suggests the presence of pathogenicity regions and genes in the M. abscessus genome.
The goal of this project is:
With this objective, Genoscope is undertaking the sequencing of the
M. abscessus CIP strain 104536T (= ATCC 19977T, knee abcess,
1953) and the M. chelonae CIP strain 104535 (= ATCC 35752T,
tortoise, 1921), which were utilized in the previously mentioned
subtractive hybridization study. Each genome will be sequenced at a
depth of 12X, using three libraries: a library of 3 kb inserts cloned
in the multicopy plasmid vector pNAV, a 10 kb library in the pCNS
plasmid vector (small copy number) and a miniBAC library (inserts of
about 30 kb) in the pBBC vector (derived from pBeloBAC11).
The shotgun stage should produce about 150,000 reads. A finishing step will increase the quality of the sequences obtained.